Rroxscaffold_1G00020410

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000682
Physical Location & Seq
Forward (+)
24696704 .. 24698162
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00020410.1

Sequence Viewer

Length: 1215 bp
ATGGGTAAGGAAAAGTCCCACATCAACATCGTGGTCATAGGCCACGTCGACTCTGGCAAGTCTACCACCACAGGTCACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAAAGGTCATTCAAGTATGCTTGGGTGTTGGACAAGCTTAAGGCTGAGCGTGAGCGTGGTATCACCATTGATATTGCCTTGTGGAAGTTTGAGACCACCAAGTACTACTGCACTGTCATCGATGCTCCCGGACATCGTGATTTCATCAAGAACATGATTACTGGTACCTCCCAGGCTGATTGCGCTGTACTCATAATCGACTCCACCACAGGTGGTTTTGAAGCTGGTATCTCCAAGGATGGTCAGACCCGTGAGCACGCTCTGCTTGCTTTCACTCTTGGTGTCAAGCAGATGATCTGTTGCTGTAACAAGATGGATGCCACCACTCCCAAGTATGCCAAGGCAAGGTACGATGAAATCGTGAAGGAAGTCTCTTCCTACCTGAAGAAGGTTGGATACAACCCAGAGAAAATTGCCTTTGTTCCCATCTCTGGATTCGAGGGCGACAACATGATTGAGAGGTCCACTAACCTTGACTGGTACAAGGGTCCTACCCTCCTTGAGGCGCTCGACCAGATCAACGAGCCAAAGAGGCCCTCAGACAAGCCTCTCCGTCTCCCACTCCAGGATGTGTACAAGATTGGTGGCATTGGAACTGTGCCTGTGGGACGTGTTGAGACTGGTCTCATCAAGCCTGAGGCTCTTCCTGGTGACAACGTTGGGTTCAATGTTAAGAATGTCTCTGTCAAGGATCTCAAGCGTGGTTATGTTGCTTCCAACTCAAAGGAAGATCCCGCAAAGGAGGCTGCCAACTTTACCTCTCAGTTCGCTGAGCTTTTGACAAAGATTGACAGGCGGTCTGGCAAGGAGCTAGAGAAGGAGCCCAAGTTCTTGAAGAATGGTGATGCTGGTATGGTTAAGATGCTTCCCACCAAGCCCATGGTTGTCGAGACCTTCTCTGAGTACCCACCTCTAGGTCGTTTTGCTGTGAGGGACATGCGCCAGACAGTTGCTGTTGGAGTCATCAAGAATGTTGAGAAGAAGGATCCAACTGGTGCTAAGGTGACCAAGTCTGCTGCTAAAAAAAGAAGTGAACCGTCATGGCCTACCCTAAGATTTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

404

Amino Acids

44.94

Weight (kDa)

9.3

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 5 - 223 3.5e-58 Elongation factor Tu GTP binding domain
GTP_EFTU_D3 PF03143 300 - 375 4.2e-14 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 301 - 373 5e-24 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 314
AccI GTMKAC 2 cut(s) 48, 62
AciI CCGC 2 cut(s) 885, 946
AclI AACGTT 1 cut(s) 807
AclWI GGATC 4 cut(s) 849, 875, 1130, 1143
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 7 cut(s) 254, 316, 339, 500, 632, 725, 1055
AfiI CCNNNNNNNGG 6 cut(s) 495, 538, 581, 652, 715, 1064
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 4 cut(s) 163, 371, 817, 985
AhdI GACNNNNNGTC 1 cut(s) 946
AjiI CACGTC 2 cut(s) 46, 761
AjnI CCWGG 3 cut(s) 321, 714, 796
AloI GAACNNNNNNTCC 2 cut(s) 962, 994
AluBI AGCT 5 cut(s) 91, 187, 374, 925, 961
AluI AGCT 5 cut(s) 91, 187, 374, 925, 961
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 7 cut(s) 236, 526, 710, 761, 779, 835, 1034
AlwI GGATC 4 cut(s) 849, 875, 1130, 1143
AlwNI CAGNNNCTG 1 cut(s) 1103
AoxI GGCC 3 cut(s) 40, 683, 1193
ApeKI GCWGC 3 cut(s) 137, 896, 1166
Asp718I GGTACC 1 cut(s) 314
AspLEI GCGC 3 cut(s) 335, 658, 1092
AspS9I GGNCC 3 cut(s) 612, 638, 684
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 4 cut(s) 205, 812, 1004, 1165
AvaII GGWCC 2 cut(s) 612, 638
AxyI CCTNAGG 1 cut(s) 786
BaeI ACNNNNGTAYC 2 cut(s) 622, 655
BamHI GGATCC 1 cut(s) 1135
BanI GGYRCC 1 cut(s) 314
BanII GRGCYC 1 cut(s) 975
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 3 cut(s) 124, 883, 1153
BccI CCATC 3 cut(s) 383, 457, 584
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 3 cut(s) 323, 716, 798
BciVI GTATCC 1 cut(s) 539
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 7 cut(s) 236, 526, 710, 761, 779, 835, 1034
BfaI CTAG 2 cut(s) 962, 1064
BfoI RGCGCY 1 cut(s) 659
BfrI CTTAAG 1 cut(s) 188
BfuI GTATCC 1 cut(s) 539
BglI GCCNNNNNGGC 1 cut(s) 682
BisI GCNGC 3 cut(s) 138, 897, 1167
BlpI GCTNAGC 2 cut(s) 195, 921
BlsI GCNGC 3 cut(s) 139, 898, 1168
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 4 cut(s) 279, 323, 716, 798
Bme18I GGWCC 2 cut(s) 612, 638
BmeRI GACNNNNNGTC 1 cut(s) 946
BmgBI CACGTC 2 cut(s) 46, 761
BmgT120I GGNCC 3 cut(s) 612, 638, 684
BmiI GGNNCC 4 cut(s) 316, 639, 972, 1137
BmrFI CCNGG 4 cut(s) 279, 323, 716, 798
BmsI GCATC 4 cut(s) 262, 457, 985, 1002
BplI GAGNNNNNCTC 2 cut(s) 1031, 1063
BpmI CTGGAG 1 cut(s) 698
Bpu10I CCTNAGC 1 cut(s) 1149
Bpu1102I GCTNAGC 2 cut(s) 195, 921
BpuEI CTTGAG 2 cut(s) 671, 830
BpuMI CCSGG 1 cut(s) 279
Bsa29I ATCGAT 1 cut(s) 270
BsaI GGTCTC 3 cut(s) 236, 779, 1034
BsaJI CCNNGG 4 cut(s) 321, 384, 489, 1029
BsaXI ACNNNNNCTCC 2 cut(s) 962, 992
Bsc4I CCNNNNNNNGG 6 cut(s) 495, 538, 581, 652, 715, 1064
Bse1I ACTGG 4 cut(s) 316, 632, 775, 1147
Bse21I CCTNAGG 1 cut(s) 786
BseBI CCWGG 3 cut(s) 323, 716, 798
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 4 cut(s) 321, 384, 489, 1029
BseGI GGATG 3 cut(s) 394, 472, 724
BseLI CCNNNNNNNGG 6 cut(s) 495, 538, 581, 652, 715, 1064
BseMII CTCAG 7 cut(s) 132, 186, 702, 777, 912, 926, 1041
BseNI ACTGG 4 cut(s) 316, 632, 775, 1147
BseXI GCAGC 3 cut(s) 124, 883, 1153
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 3 cut(s) 42, 685, 1195
BshNI GGYRCC 1 cut(s) 314
BshVI ATCGAT 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 1 cut(s) 279
BslFI GGGAC 2 cut(s) 771, 1097
BslI CCNNNNNNNGG 6 cut(s) 495, 538, 581, 652, 715, 1064
BsmAI GTCTC 7 cut(s) 236, 526, 710, 761, 779, 835, 1034
BsmBI CGTCTC 1 cut(s) 710
BsmFI GGGAC 2 cut(s) 771, 1097
BsnI GGCC 3 cut(s) 42, 685, 1195
Bso31I GGTCTC 3 cut(s) 236, 779, 1034
Bsp1286I GDGCHC 2 cut(s) 408, 975
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 7 cut(s) 81, 114, 444, 666, 841, 880, 1135
Bsp1720I GCTNAGC 2 cut(s) 195, 921
Bsp19I CCATGG 1 cut(s) 1029
BspACI CCGC 2 cut(s) 885, 946
BspANI GGCC 3 cut(s) 42, 685, 1195
BspCNI CTCAG 7 cut(s) 133, 187, 701, 778, 913, 925, 1042
BspDI ATCGAT 1 cut(s) 270
BspLI GGNNCC 4 cut(s) 316, 639, 972, 1137
BspPI GGATC 4 cut(s) 849, 875, 1130, 1143
BspQI GCTCTTC 1 cut(s) 798
BspT107I GGYRCC 1 cut(s) 314
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 3 cut(s) 236, 779, 1034
BsrGI TGTACA 1 cut(s) 723
BsrI ACTGG 4 cut(s) 316, 632, 775, 1147
BssECI CCNNGG 4 cut(s) 321, 384, 489, 1029
BssMI GATC 7 cut(s) 81, 114, 444, 666, 841, 880, 1135
BssT1I CCWWGG 3 cut(s) 384, 489, 1029
Bst2UI CCWGG 3 cut(s) 323, 716, 798
Bst4CI ACNGT 4 cut(s) 265, 748, 1099, 1188
Bst6I CTCTTC 2 cut(s) 529, 798
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 723
BstC8I GCNNGC 2 cut(s) 408, 417
BstDEI CTNAG 9 cut(s) 141, 195, 688, 786, 912, 921, 1050, 1149, 1202
BstDSI CCRYGG 1 cut(s) 1029
BstEII GGTNACC 1 cut(s) 1153
BstENI CCTNNNNNAGG 2 cut(s) 536, 650
BstF5I GGATG 3 cut(s) 394, 472, 724
BstH2I RGCGCY 1 cut(s) 659
BstHHI GCGC 3 cut(s) 335, 658, 1092
BstKTI GATC 7 cut(s) 84, 117, 447, 669, 844, 883, 1138
BstMAI GTCTC 7 cut(s) 236, 526, 710, 761, 779, 835, 1034
BstMBI GATC 7 cut(s) 81, 114, 444, 666, 841, 880, 1135
BstMWI GCNNNNNNNGC 5 cut(s) 332, 412, 416, 682, 893
BstNI CCWGG 3 cut(s) 323, 716, 798
BstNSI RCATGY 1 cut(s) 1090
BstPI GGTNACC 1 cut(s) 1153
BstSCI CCNGG 4 cut(s) 277, 321, 714, 796
BstV1I GCAGC 3 cut(s) 124, 883, 1153
BstX2I RGATCY 3 cut(s) 841, 880, 1135
BstXI CCANNNNNNTGG 1 cut(s) 1030
BstYI RGATCY 3 cut(s) 841, 880, 1135
Bsu15I ATCGAT 1 cut(s) 270
Bsu36I CCTNAGG 1 cut(s) 786
BsuI GTATCC 1 cut(s) 539
BsuRI GGCC 3 cut(s) 42, 685, 1195
BsuTUI ATCGAT 1 cut(s) 270
BtgI CCRYGG 1 cut(s) 1029
BtrI CACGTC 2 cut(s) 46, 761
BtsCI GGATG 3 cut(s) 394, 472, 724
BtsIMutI CAGTG 1 cut(s) 261
Cac8I GCNNGC 2 cut(s) 408, 417
CaiI CAGNNNCTG 1 cut(s) 1103
CfoI GCGC 3 cut(s) 335, 658, 1092
Cfr13I GGNCC 3 cut(s) 612, 638, 684
ClaI ATCGAT 1 cut(s) 270
Csp6I GTAC 7 cut(s) 253, 315, 338, 499, 631, 724, 1054
CspCI CAANNNNNGTGG 6 cut(s) 76, 111, 604, 639, 715, 750
CviAII CATG 5 cut(s) 304, 601, 1030, 1087, 1191
CviQI GTAC 7 cut(s) 253, 315, 338, 499, 631, 724, 1054
DdeI CTNAG 9 cut(s) 141, 195, 688, 786, 912, 921, 1050, 1149, 1202
DpnI GATC 7 cut(s) 83, 116, 446, 668, 843, 882, 1137
DpnII GATC 7 cut(s) 81, 114, 444, 666, 841, 880, 1135
DriI GACNNNNNGTC 1 cut(s) 946
Eam1104I CTCTTC 2 cut(s) 529, 798
Eam1105I GACNNNNNGTC 1 cut(s) 946
EarI CTCTTC 2 cut(s) 529, 798
Eco130I CCWWGG 3 cut(s) 384, 489, 1029
Eco24I GRGCYC 1 cut(s) 975
Eco31I GGTCTC 3 cut(s) 236, 779, 1034
Eco47I GGWCC 2 cut(s) 612, 638
Eco57I CTGAAG 1 cut(s) 554
Eco81I CCTNAGG 1 cut(s) 786
Eco91I GGTNACC 1 cut(s) 1153
EcoNI CCTNNNNNAGG 2 cut(s) 536, 650
EcoO109I RGGNCCY 2 cut(s) 638, 684
EcoO65I GGTNACC 1 cut(s) 1153
EcoRII CCWGG 3 cut(s) 321, 714, 796
EcoT14I CCWWGG 3 cut(s) 384, 489, 1029
EcoT38I GRGCYC 1 cut(s) 975
ErhI CCWWGG 3 cut(s) 384, 489, 1029
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 5 cut(s) 307, 604, 1033, 1090, 1194
FaqI GGGAC 2 cut(s) 771, 1097
FatI CATG 5 cut(s) 303, 600, 1029, 1086, 1190
FauI CCCGC 1 cut(s) 892
FblI GTMKAC 2 cut(s) 48, 62
Fnu4HI GCNGC 3 cut(s) 138, 897, 1167
FokI GGATG 3 cut(s) 401, 479, 731
FriOI GRGCYC 1 cut(s) 975
Fsp4HI GCNGC 3 cut(s) 138, 897, 1167
FspBI CTAG 2 cut(s) 962, 1064
GlaI GCGC 3 cut(s) 334, 657, 1091
GluI GCNGC 3 cut(s) 138, 897, 1167
GsuI CTGGAG 1 cut(s) 698
HaeII RGCGCY 1 cut(s) 659
HaeIII GGCC 3 cut(s) 42, 685, 1195
HapII CCGG 1 cut(s) 279
HhaI GCGC 3 cut(s) 335, 658, 1092
Hin1II CATG 5 cut(s) 307, 604, 1033, 1090, 1194
Hin6I GCGC 3 cut(s) 333, 656, 1090
HinP1I GCGC 3 cut(s) 333, 656, 1090
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 89, 185
HinfI GANTC 4 cut(s) 50, 350, 585, 1110
HpaII CCGG 1 cut(s) 279
HphI GGTGA 4 cut(s) 205, 812, 1004, 1165
Hpy166II GTNNAC 5 cut(s) 49, 63, 615, 724, 1184
Hpy188I TCNGA 3 cut(s) 396, 691, 1051
Hpy188III TCNNGA 9 cut(s) 118, 127, 287, 298, 511, 582, 982, 1039, 1117
Hpy8I GTNNAC 5 cut(s) 49, 63, 615, 724, 1184
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 6 cut(s) 124, 508, 532, 961, 1054, 1126
HpyCH4III ACNGT 4 cut(s) 265, 748, 1099, 1188
HpyCH4IV ACGT 3 cut(s) 45, 760, 807
HpyCH4V TGCA 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 5 cut(s) 332, 412, 416, 682, 893
HpyF3I CTNAG 9 cut(s) 141, 195, 688, 786, 912, 921, 1050, 1149, 1202
HpySE526I ACGT 3 cut(s) 45, 760, 807
Hsp92II CATG 5 cut(s) 307, 604, 1033, 1090, 1194
HspAI GCGC 3 cut(s) 333, 656, 1090
KpnI GGTACC 1 cut(s) 318
Kzo9I GATC 7 cut(s) 81, 114, 444, 666, 841, 880, 1135
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 3 cut(s) 280, 958, 970
Lsp1109I GCAGC 3 cut(s) 124, 883, 1153
LweI GCATC 4 cut(s) 262, 457, 985, 1002
MaeI CTAG 2 cut(s) 962, 1064
MaeII ACGT 3 cut(s) 45, 760, 807
MaeIII GTNAC 4 cut(s) 74, 455, 800, 1153
MalI GATC 7 cut(s) 83, 116, 446, 668, 843, 882, 1137
MboI GATC 7 cut(s) 81, 114, 444, 666, 841, 880, 1135
MboII GAAGA 6 cut(s) 516, 547, 785, 890, 997, 1141
MflI RGATCY 3 cut(s) 841, 880, 1135
MhlI GDGCHC 2 cut(s) 408, 975
MluCI AATT 1 cut(s) 561
MlyI GAGTC 3 cut(s) 44, 344, 1119
MmeI TCCRAC 5 cut(s) 159, 523, 891, 1087, 1163
MseI TTAA 3 cut(s) 189, 822, 1008
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 4 cut(s) 279, 323, 716, 798
MvaI CCWGG 3 cut(s) 323, 716, 798
MwoI GCNNNNNNNGC 5 cut(s) 332, 412, 416, 682, 893
NciI CCSGG 1 cut(s) 279
NcoI CCATGG 1 cut(s) 1029
NdeII GATC 7 cut(s) 81, 114, 444, 666, 841, 880, 1135
NlaIII CATG 5 cut(s) 307, 604, 1033, 1090, 1194
NlaIV GGNNCC 4 cut(s) 316, 639, 972, 1137
NmuCI GTSAC 3 cut(s) 74, 800, 1153
NspI RCATGY 1 cut(s) 1090
PciSI GCTCTTC 1 cut(s) 798
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 507
PfeI GAWTC 1 cut(s) 585
PflFI GACNNNGTC 1 cut(s) 1159
PfoI TCCNGGA 2 cut(s) 277, 714
PkrI GCNGC 3 cut(s) 139, 898, 1168
PleI GAGTC 3 cut(s) 44, 344, 1118
PpsI GAGTC 3 cut(s) 44, 344, 1118
PpuMI RGGWCCY 1 cut(s) 638
Psp1406I AACGTT 1 cut(s) 807
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 3 cut(s) 321, 714, 796
PspEI GGTNACC 1 cut(s) 1153
PspGI CCWGG 3 cut(s) 321, 714, 796
PspN4I GGNNCC 4 cut(s) 316, 639, 972, 1137
PspPI GGNCC 3 cut(s) 612, 638, 684
PspPPI RGGWCCY 1 cut(s) 638
PstNI CAGNNNCTG 1 cut(s) 1103
PsuI RGATCY 3 cut(s) 841, 880, 1135
PsyI GACNNNGTC 1 cut(s) 1159
RsaI GTAC 7 cut(s) 254, 316, 339, 500, 632, 725, 1055
RsaNI GTAC 7 cut(s) 253, 315, 338, 499, 631, 724, 1054
SalI GTCGAC 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 798
SaqAI TTAA 3 cut(s) 189, 822, 1008
SatI GCNGC 3 cut(s) 138, 897, 1167
Sau3AI GATC 7 cut(s) 81, 114, 444, 666, 841, 880, 1135
Sau96I GGNCC 3 cut(s) 612, 638, 684
ScaI AGTACT 1 cut(s) 254
SchI GAGTC 3 cut(s) 44, 344, 1119
ScrFI CCNGG 4 cut(s) 279, 323, 716, 798
SduI GDGCHC 2 cut(s) 408, 975
SfaNI GCATC 4 cut(s) 262, 457, 985, 1002
SinI GGWCC 2 cut(s) 612, 638
SmlI CTYRAG 3 cut(s) 188, 650, 845
SmoI CTYRAG 3 cut(s) 188, 650, 845
Sse9I AATT 1 cut(s) 561
SsiI CCGC 2 cut(s) 885, 946
SspMI CTAG 2 cut(s) 962, 1064
StyD4I CCNGG 4 cut(s) 277, 321, 714, 796
StyI CCWWGG 3 cut(s) 384, 489, 1029
TaaI ACNGT 4 cut(s) 265, 748, 1099, 1188
TaiI ACGT 3 cut(s) 48, 763, 810
TaqI TCGA 8 cut(s) 48, 117, 126, 270, 348, 588, 660, 1038
TasI AATT 1 cut(s) 561
TatI WGTACW 3 cut(s) 252, 337, 723
TfiI GAWTC 1 cut(s) 585
Tru1I TTAA 3 cut(s) 189, 822, 1008
Tru9I TTAA 3 cut(s) 189, 822, 1008
TscAI CASTG 1 cut(s) 268
TseFI GTSAC 3 cut(s) 74, 800, 1153
TseI GCWGC 3 cut(s) 137, 896, 1166
Tsp45I GTSAC 3 cut(s) 74, 800, 1153
TspDTI ATGAA 3 cut(s) 161, 283, 519
TspGWI ACGGA 2 cut(s) 692, 1200
TspRI CASTG 1 cut(s) 268
Tth111I GACNNNGTC 1 cut(s) 1159
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 612, 638
XagI CCTNNNNNAGG 2 cut(s) 536, 650
XceI RCATGY 1 cut(s) 1090
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 1027
XmiI GTMKAC 2 cut(s) 48, 62
XspI CTAG 2 cut(s) 962, 1064
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.