Rw1G028370

Elongation factor Tu C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000682
Physical Location & Seq
Reverse (-)
56045439 .. 56046925
1487 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G028370.1

Sequence Viewer

Length: 1209 bp
ATGGGCAAGGAAAAGTTTCATATCAACATTGTGGTTATTGGCCATGTTGACTCTGGGAAGTCGACCACACTGTATGCTTGGGTGCTTGACAAGCTTAAGGCTGAGCGTGAAAGGGGTATTACCATTGACATTGCTCTCTGGAAGTTTGAGACCACCAATACTACTAACATGATTACTGGAACTTCCCAGGCTGATTGTGCTGTCCTCATTATTGACTCTACCACTGGAGGTTTTGAGGCTGGTATCTCTAAGGATGGTCAGACCCGTGAGCATGCTCTCCTTGCTTTCACCCTTGGTGTTAGGCAAATGATTTGTTGCTGCAACAAGATGGATGCTACCAGTCCTAAGTACTCGAAGGCAAGGTATGATGAAATCGTGAAGGAAGTGTCATCCTATCTGAAGAAGATTGGATACAACCCAGACAAAATTGCTTTTGTTCCCATCTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTCGACTGGTACAAGGGTCCAACCCTTCTTGAGGCCCTTGACATGATCAACGAGCCCAAGAGGCCCTCCGACAAGCCCCTCCGACTTCCACTTCAGGATGTCTACAAGATTGGAGGTATTGGAACTGTTCCTGTTGGACGTGTGGAGACTGGTATTATCAAGCCTGGTATGGTTGTGACTTTTGCTCCCACTGGGTTGACCACTGAAGTTAAATCTGTGGAGATGCACCACGAGGCTCTCCAGGAGGCTCTTCCCGGTGATAATGTTGGCTTCAACGTCAAGAATGTTGCAGTCAAGGATCTCAAGCGTGGGTATGTTGCTTCAAACTCCAAGGATGATCCAGCCAAGGAAGCAGCTAACTTCACTGCCCAGGTCATCATTATGAATCACCCTGGCCAGATTGGAAACGGTTATGCTCCTGTGCTTGATTGCCACACCTCTCACATTGCTGTCAAGTTCAATGAGATTTTGACCAAGATTGACAGACGTTCTGGCAAGGAGCTAGAGAAGGAGCCCAAGTTTTTGAAGAATGGTGATGCAGGGTTTGTTAAGATGATTCCCACCAAGCCCATGGTCGTTGAAACTTTCTCTGAGTATCCCCCACTTGGTCGTTTTGCTGTGAGGGACATGCGCCAGACAGTTGCTGTGGGAGTCATCAAGGCTGTGGAGAAGAAGGATCCCAGCGGAGCCAAGGTTACCAAGTCTGCAGCCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

402

Amino Acids

44.1

Weight (kDa)

8.99

Isoelectric Point (pI)

29.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 176 2.9e-28 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 203 - 268 2.2e-17 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 277 - 384 1.6e-27 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 282 - 382 4.1e-38 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 62, 588
AciI CCGC 1 cut(s) 1170
AclWI GGATC 4 cut(s) 792, 818, 1157, 1170
AcoI YGGCCR 2 cut(s) 40, 880
AcuI CTGAAG 3 cut(s) 419, 563, 711
AfaI GTAC 2 cut(s) 350, 497
AfiI CCNNNNNNNGG 3 cut(s) 446, 517, 1091
AflII CTTAAG 1 cut(s) 95
AflIII ACRYGT 1 cut(s) 625
AgsI TTSAA 5 cut(s) 760, 810, 946, 1012, 1067
AjiI CACGTC 1 cut(s) 626
AjnI CCWGG 5 cut(s) 186, 649, 726, 855, 877
AluBI AGCT 3 cut(s) 94, 842, 988
AluI AGCT 3 cut(s) 94, 842, 988
Alw26I GTCTC 2 cut(s) 143, 626
AlwI GGATC 4 cut(s) 792, 818, 1157, 1170
AlwNI CAGNNNCTG 1 cut(s) 1130
AoxI GGCC 4 cut(s) 40, 519, 548, 880
ApeKI GCWGC 3 cut(s) 318, 839, 1193
Asp700I GAANNNNTTC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 1119
AspS9I GGNCC 4 cut(s) 477, 503, 520, 549
AsuC2I CCSGG 1 cut(s) 741
AsuHPI GGTGA 5 cut(s) 280, 470, 755, 866, 1031
AvaII GGWCC 2 cut(s) 477, 503
BaeI ACNNNNGTAYC 2 cut(s) 487, 520
BalI TGGCCA 2 cut(s) 42, 882
BamHI GGATCC 1 cut(s) 1162
BanII GRGCYC 2 cut(s) 543, 1002
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BauI CACGAG 1 cut(s) 716
BbvI GCAGC 3 cut(s) 305, 851, 1205
BccI CCATC 3 cut(s) 248, 322, 449
BciT130I CCWGG 5 cut(s) 188, 651, 728, 857, 879
BciVI GTATCC 2 cut(s) 404, 1092
BclI TGATCA 1 cut(s) 531
BcnI CCSGG 1 cut(s) 741
BcoDI GTCTC 2 cut(s) 143, 626
BfaI CTAG 1 cut(s) 989
BfmI CTRYAG 1 cut(s) 1191
BfrI CTTAAG 1 cut(s) 95
BfuI GTATCC 2 cut(s) 404, 1092
BglI GCCNNNNNGGC 1 cut(s) 547
BisI GCNGC 3 cut(s) 319, 840, 1194
BlpI GCTNAGC 1 cut(s) 102
BlsI GCNGC 3 cut(s) 320, 841, 1195
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 6 cut(s) 188, 651, 728, 741, 857, 879
Bme18I GGWCC 2 cut(s) 477, 503
BmgBI CACGTC 1 cut(s) 626
BmgT120I GGNCC 4 cut(s) 477, 503, 520, 549
BmiI GGNNCC 4 cut(s) 504, 999, 1164, 1174
BmrFI CCNGG 6 cut(s) 188, 651, 728, 741, 857, 879
BmrI ACTGGG 1 cut(s) 687
BmsI GCATC 3 cut(s) 322, 699, 1012
BmuI ACTGGG 1 cut(s) 687
BpmI CTGGAG 2 cut(s) 246, 710
Bpu1102I GCTNAGC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 536, 773
BpuMI CCSGG 1 cut(s) 741
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 8 cut(s) 186, 292, 816, 831, 855, 877, 1056, 1176
BsaXI ACNNNNNCTCC 6 cut(s) 655, 685, 989, 1019, 1164, 1194
Bsc4I CCNNNNNNNGG 3 cut(s) 446, 517, 1091
Bse1I ACTGG 6 cut(s) 181, 229, 339, 497, 640, 682
Bse3DI GCAATG 2 cut(s) 129, 930
BseBI CCWGG 5 cut(s) 188, 651, 728, 857, 879
BseDI CCNNGG 8 cut(s) 186, 292, 816, 831, 855, 877, 1056, 1176
BseGI GGATG 5 cut(s) 259, 337, 389, 589, 826
BseLI CCNNNNNNNGG 3 cut(s) 446, 517, 1091
BseMI GCAATG 2 cut(s) 129, 930
BseMII CTCAG 2 cut(s) 93, 1068
BseNI ACTGG 6 cut(s) 181, 229, 339, 497, 640, 682
BseXI GCAGC 3 cut(s) 305, 851, 1205
BseYI CCCAGC 1 cut(s) 1166
BshFI GGCC 4 cut(s) 42, 521, 550, 882
BsiSI CCGG 1 cut(s) 741
BslFI GGGAC 1 cut(s) 1124
BslI CCNNNNNNNGG 3 cut(s) 446, 517, 1091
BsmAI GTCTC 2 cut(s) 143, 626
BsmFI GGGAC 1 cut(s) 1124
BsnI GGCC 4 cut(s) 42, 521, 550, 882
Bso31I GGTCTC 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 543, 1002
Bsp143I GATC 4 cut(s) 531, 784, 823, 1162
Bsp1720I GCTNAGC 1 cut(s) 102
Bsp19I CCATGG 1 cut(s) 1056
BspACI CCGC 1 cut(s) 1170
BspANI GGCC 4 cut(s) 42, 521, 550, 882
BspCNI CTCAG 2 cut(s) 94, 1069
BspLI GGNNCC 4 cut(s) 504, 999, 1164, 1174
BspMAI CTGCAG 1 cut(s) 1195
BspPI GGATC 4 cut(s) 792, 818, 1157, 1170
BspQI GCTCTTC 1 cut(s) 741
BspTI CTTAAG 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 129, 930
BsrI ACTGG 6 cut(s) 181, 229, 339, 497, 640, 682
BssECI CCNNGG 8 cut(s) 186, 292, 816, 831, 855, 877, 1056, 1176
BssMI GATC 4 cut(s) 531, 784, 823, 1162
BssSI CACGAG 1 cut(s) 716
BssT1I CCWWGG 5 cut(s) 292, 816, 831, 1056, 1176
Bst2BI CACGAG 1 cut(s) 716
Bst2UI CCWGG 5 cut(s) 188, 651, 728, 857, 879
Bst4CI ACNGT 4 cut(s) 72, 613, 896, 1126
Bst6I CTCTTC 1 cut(s) 741
BstAFI CTTAAG 1 cut(s) 95
BstC8I GCNNGC 1 cut(s) 273
BstDEI CTNAG 4 cut(s) 102, 249, 345, 1077
BstDSI CCRYGG 1 cut(s) 1056
BstEII GGTNACC 1 cut(s) 1180
BstENI CCTNNNNNAGG 1 cut(s) 515
BstF5I GGATG 5 cut(s) 259, 337, 389, 589, 826
BstHHI GCGC 1 cut(s) 1119
BstKTI GATC 4 cut(s) 534, 787, 826, 1165
BstMAI GTCTC 2 cut(s) 143, 626
BstMBI GATC 4 cut(s) 531, 784, 823, 1162
BstMWI GCNNNNNNNGC 5 cut(s) 91, 197, 281, 547, 836
BstNI CCWGG 5 cut(s) 188, 651, 728, 857, 879
BstNSI RCATGY 2 cut(s) 275, 1117
BstPI GGTNACC 1 cut(s) 1180
BstSCI CCNGG 6 cut(s) 186, 649, 726, 739, 855, 877
BstSFI CTRYAG 1 cut(s) 1191
BstV1I GCAGC 3 cut(s) 305, 851, 1205
BstX2I RGATCY 2 cut(s) 784, 1162
BstXI CCANNNNNNTGG 1 cut(s) 1057
BstYI RGATCY 2 cut(s) 784, 1162
BsuI GTATCC 2 cut(s) 404, 1092
BsuRI GGCC 4 cut(s) 42, 521, 550, 882
BtgI CCRYGG 1 cut(s) 1056
BtrI CACGTC 1 cut(s) 626
BtsCI GGATG 5 cut(s) 259, 337, 389, 589, 826
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 5 cut(s) 68, 222, 675, 687, 849
Cac8I GCNNGC 1 cut(s) 273
CaiI CAGNNNCTG 1 cut(s) 1130
CfoI GCGC 1 cut(s) 1119
Cfr13I GGNCC 4 cut(s) 477, 503, 520, 549
Csp6I GTAC 2 cut(s) 349, 496
CviAII CATG 7 cut(s) 44, 169, 272, 466, 529, 1057, 1114
CviQI GTAC 2 cut(s) 349, 496
DdeI CTNAG 4 cut(s) 102, 249, 345, 1077
DpnI GATC 4 cut(s) 533, 786, 825, 1164
DpnII GATC 4 cut(s) 531, 784, 823, 1162
EaeI YGGCCR 2 cut(s) 40, 880
Eam1104I CTCTTC 1 cut(s) 741
EarI CTCTTC 1 cut(s) 741
Eco130I CCWWGG 5 cut(s) 292, 816, 831, 1056, 1176
Eco24I GRGCYC 2 cut(s) 543, 1002
Eco31I GGTCTC 1 cut(s) 143
Eco47I GGWCC 2 cut(s) 477, 503
Eco57I CTGAAG 3 cut(s) 419, 563, 711
Eco91I GGTNACC 1 cut(s) 1180
EcoNI CCTNNNNNAGG 1 cut(s) 515
EcoO109I RGGNCCY 2 cut(s) 520, 549
EcoO65I GGTNACC 1 cut(s) 1180
EcoRII CCWGG 5 cut(s) 186, 649, 726, 855, 877
EcoT14I CCWWGG 5 cut(s) 292, 816, 831, 1056, 1176
EcoT38I GRGCYC 2 cut(s) 543, 1002
ErhI CCWWGG 5 cut(s) 292, 816, 831, 1056, 1176
FaeI CATG 7 cut(s) 47, 172, 275, 469, 532, 1060, 1117
FaqI GGGAC 1 cut(s) 1124
FatI CATG 7 cut(s) 43, 168, 271, 465, 528, 1056, 1113
FbaI TGATCA 1 cut(s) 531
FblI GTMKAC 2 cut(s) 62, 588
Fnu4HI GCNGC 3 cut(s) 319, 840, 1194
FokI GGATG 5 cut(s) 266, 344, 376, 596, 833
FriOI GRGCYC 2 cut(s) 543, 1002
Fsp4HI GCNGC 3 cut(s) 319, 840, 1194
FspBI CTAG 1 cut(s) 989
GlaI GCGC 1 cut(s) 1118
GluI GCNGC 3 cut(s) 319, 840, 1194
GsaI CCCAGC 1 cut(s) 1170
GsuI CTGGAG 2 cut(s) 246, 710
HaeIII GGCC 4 cut(s) 42, 521, 550, 882
HapII CCGG 1 cut(s) 741
HhaI GCGC 1 cut(s) 1119
Hin1II CATG 7 cut(s) 47, 172, 275, 469, 532, 1060, 1117
Hin6I GCGC 1 cut(s) 1117
HinP1I GCGC 1 cut(s) 1117
HincII GTYRAC 3 cut(s) 49, 63, 684
HindII GTYRAC 3 cut(s) 49, 63, 684
HindIII AAGCTT 1 cut(s) 92
HinfI GANTC 5 cut(s) 50, 215, 871, 1042, 1137
HpaII CCGG 1 cut(s) 741
HphI GGTGA 5 cut(s) 280, 470, 755, 866, 1031
Hpy166II GTNNAC 5 cut(s) 49, 63, 480, 589, 684
Hpy188I TCNGA 5 cut(s) 261, 399, 556, 569, 1078
Hpy188III TCNNGA 6 cut(s) 139, 376, 447, 515, 581, 766
Hpy8I GTNNAC 5 cut(s) 49, 63, 480, 589, 684
HpyAV CCTTC 5 cut(s) 349, 373, 521, 988, 1153
HpyCH4III ACNGT 4 cut(s) 72, 613, 896, 1126
HpyCH4IV ACGT 3 cut(s) 625, 762, 973
HpyCH4V TGCA 5 cut(s) 321, 712, 776, 1025, 1193
HpyF10VI GCNNNNNNNGC 5 cut(s) 91, 197, 281, 547, 836
HpyF3I CTNAG 4 cut(s) 102, 249, 345, 1077
HpySE526I ACGT 3 cut(s) 625, 762, 973
Hsp92II CATG 7 cut(s) 47, 172, 275, 469, 532, 1060, 1117
HspAI GCGC 1 cut(s) 1117
Ksp22I TGATCA 1 cut(s) 531
Kzo9I GATC 4 cut(s) 531, 784, 823, 1162
LguI GCTCTTC 1 cut(s) 741
LmnI GCTCC 5 cut(s) 676, 907, 985, 997, 1172
Lsp1109I GCAGC 3 cut(s) 305, 851, 1205
LweI GCATC 3 cut(s) 322, 699, 1012
MaeI CTAG 1 cut(s) 989
MaeII ACGT 3 cut(s) 625, 762, 973
MaeIII GTNAC 3 cut(s) 458, 661, 1180
MalI GATC 4 cut(s) 533, 786, 825, 1164
MboI GATC 4 cut(s) 531, 784, 823, 1162
MboII GAAGA 5 cut(s) 412, 415, 728, 1024, 1168
MflI RGATCY 2 cut(s) 784, 1162
MhlI GDGCHC 2 cut(s) 543, 1002
MlsI TGGCCA 2 cut(s) 42, 882
MluCI AATT 1 cut(s) 426
MluNI TGGCCA 2 cut(s) 42, 882
MlyI GAGTC 3 cut(s) 44, 209, 1146
MmeI TCCRAC 4 cut(s) 530, 579, 592, 601
Mox20I TGGCCA 2 cut(s) 42, 882
MroXI GAANNNNTTC 1 cut(s) 15
MscI TGGCCA 2 cut(s) 42, 882
MseI TTAA 3 cut(s) 96, 696, 1035
MslI CAYNNNNRTG 1 cut(s) 866
Msp20I TGGCCA 2 cut(s) 42, 882
MspA1I CMGCKG 1 cut(s) 1170
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 1 cut(s) 741
MspR9I CCNGG 6 cut(s) 188, 651, 728, 741, 857, 879
MvaI CCWGG 5 cut(s) 188, 651, 728, 857, 879
MwoI GCNNNNNNNGC 5 cut(s) 91, 197, 281, 547, 836
NciI CCSGG 1 cut(s) 741
NcoI CCATGG 1 cut(s) 1056
NdeII GATC 4 cut(s) 531, 784, 823, 1162
NlaIII CATG 7 cut(s) 47, 172, 275, 469, 532, 1060, 1117
NlaIV GGNNCC 4 cut(s) 504, 999, 1164, 1174
NmuCI GTSAC 2 cut(s) 458, 661
NspI RCATGY 2 cut(s) 275, 1117
PaeI GCATGC 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 871, 1042
PfoI TCCNGGA 1 cut(s) 726
PkrI GCNGC 3 cut(s) 320, 841, 1195
PleI GAGTC 3 cut(s) 44, 209, 1145
PpsI GAGTC 3 cut(s) 44, 209, 1145
Psp6I CCWGG 5 cut(s) 186, 649, 726, 855, 877
PspEI GGTNACC 1 cut(s) 1180
PspFI CCCAGC 1 cut(s) 1166
PspGI CCWGG 5 cut(s) 186, 649, 726, 855, 877
PspN4I GGNNCC 4 cut(s) 504, 999, 1164, 1174
PspPI GGNCC 4 cut(s) 477, 503, 520, 549
PstI CTGCAG 1 cut(s) 1195
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 2 cut(s) 784, 1162
RsaI GTAC 2 cut(s) 350, 497
RsaNI GTAC 2 cut(s) 349, 496
RseI CAYNNNNRTG 1 cut(s) 866
SalI GTCGAC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 741
SaqAI TTAA 3 cut(s) 96, 696, 1035
SatI GCNGC 3 cut(s) 319, 840, 1194
Sau3AI GATC 4 cut(s) 531, 784, 823, 1162
Sau96I GGNCC 4 cut(s) 477, 503, 520, 549
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 3 cut(s) 44, 209, 1146
ScrFI CCNGG 6 cut(s) 188, 651, 728, 741, 857, 879
SduI GDGCHC 2 cut(s) 543, 1002
SfaNI GCATC 3 cut(s) 322, 699, 1012
SfcI CTRYAG 1 cut(s) 1191
SinI GGWCC 2 cut(s) 477, 503
SmiMI CAYNNNNRTG 1 cut(s) 866
SmlI CTYRAG 3 cut(s) 95, 515, 788
SmoI CTYRAG 3 cut(s) 95, 515, 788
SphI GCATGC 1 cut(s) 275
Sse9I AATT 1 cut(s) 426
SsiI CCGC 1 cut(s) 1170
SspMI CTAG 1 cut(s) 989
StyD4I CCNGG 6 cut(s) 186, 649, 726, 739, 855, 877
StyI CCWWGG 5 cut(s) 292, 816, 831, 1056, 1176
TaaI ACNGT 4 cut(s) 72, 613, 896, 1126
TaiI ACGT 3 cut(s) 628, 765, 976
TaqI TCGA 3 cut(s) 62, 353, 489
TasI AATT 1 cut(s) 426
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 2 cut(s) 871, 1042
Tru1I TTAA 3 cut(s) 96, 696, 1035
Tru9I TTAA 3 cut(s) 96, 696, 1035
TscAI CASTG 5 cut(s) 75, 229, 682, 694, 856
TseFI GTSAC 2 cut(s) 458, 661
TseI GCWGC 3 cut(s) 318, 839, 1193
Tsp45I GTSAC 2 cut(s) 458, 661
TspDTI ATGAA 3 cut(s) 8, 384, 884
TspRI CASTG 5 cut(s) 75, 229, 682, 694, 856
Vha464I CTTAAG 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 477, 503
XagI CCTNNNNNAGG 1 cut(s) 515
XceI RCATGY 2 cut(s) 275, 1117
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 1054
XmiI GTMKAC 2 cut(s) 62, 588
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 1 cut(s) 989
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.