RLG00000027528

Elongation factor Tu C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000682
Physical Location & Seq
Forward (+)
12440129 .. 12441619
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027528

Sequence Viewer

Length: 1344 bp
ATGGGCAAGGAAAAGTTTCATATCAACATTGTGGTTATTGGCCATGTTGACTCTGGGAAGTCGACCACCACTGGTCATTTGATCTACAAGCTTGGGGGTATTGACAAGCGTGTGATTGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGCGTTCATTCAAGTATGCTTGGGTGCTTGACAAGCTTAAGGCTGAGCGTGAAAGGGGTATTACCATTGACATTGCTCTCTGGAAGTTTGAGACCACCAAGTACTACTGCACTGTCATTGATGCTCCTGGACATCGTGACTTCATCAAGAACATGATTACTGGAACTTCCCAGGCTGATTGTGCTGTCCTCATTATTGACTCTACCACTGGAGGTTTTGAGGCTGGTATCTCTAAGGATGGTCAGACCCGTGAGCATGCTCTCCTTGCTTTCACCCTTGGTGTTAGGCAAATGATTTGTTGCTGCAACAAGATGGATGCTACCAGTCCCAAGTACTCGAAGGCAAGGTATGATGAAATCGTGAAGGAAGTGTCATCCTATCTGAAGAAGATTGGATACAACCCAGACAAAATAGCTTTTGTTCCCATCTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTCGACTGGTACAAGGGTCCAACCCTTCTTGAGGCCCTTGACATGATCAATGAGCCCAAGAGGCCCTCCGACAAGCCCCTCCGACTTCCACTTCAGGACGTCTACAAGATTGGAGGTATTGGAACTGTTCCCGTTGGACGTGTTGAGACTGGTATTATCAAGCCTGGTATGGTAGTGACTTTTGCTCCCACTGGATTGACCACTGAAGTCAAGTCTGTGGAGATGCACCACGAGGCTCTCCAGGAGGCTCTTCCCGGTGACAATGTTGGCTTCAACGTCAAGAACGTTGCAGTCAAGGATCTCAAGCGTGGGTATGTTGCTTCAAACTCCAAGGAAGATCCAGCCAAGGAAGCAGCTAACTTTACTGCCCAGGTCATCATTATGAATCACCCTGGCCAGATTGGAAACGGTTATGCTCCTGTGCTTGACTGCCACACCTCTCACATTGCTGTCAAGTTCAACGAGATTTTGACCAAGATTGACAGACGTTCTGGCAAGGAGCTCGAGAAGGAGCCCAAGTTTTTGAAGAATGGTGACGCAGGGTTTGTTAAAATGATTCCCACCAAGCCCATGGTCGTTGAAACTTTCTCCGAGTATCCCCCACTTGGTCGTTTTGCTGTGAGGGACATGCGCCAGACAGTTGCTGTGGGAGTCATCAAGGCTGTGGAGAAGAAGGATCCAAGCGGAGCCAAGGTTACCAAGTCTGCGGCCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

448

Amino Acids

49.44

Weight (kDa)

9.15

Isoelectric Point (pI)

32.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 221 3.6e-57 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 248 - 313 2.6e-17 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 322 - 429 2e-27 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 327 - 427 5e-38 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 723
AccI GTMKAC 2 cut(s) 62, 723
AciI CCGC 2 cut(s) 1305, 1328
AclI AACGTT 1 cut(s) 906
AclWI GGATC 4 cut(s) 927, 953, 1292, 1305
AcoI YGGCCR 3 cut(s) 40, 1015, 1329
AcuI CTGAAG 3 cut(s) 554, 698, 846
AcyI GRCGYC 1 cut(s) 720
AfaI GTAC 3 cut(s) 254, 485, 632
AfiI CCNNNNNNNGG 3 cut(s) 581, 652, 1226
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 6 cut(s) 163, 895, 945, 1081, 1147, 1202
AjiI CACGTC 1 cut(s) 761
AjnI CCWGG 6 cut(s) 277, 321, 784, 861, 990, 1012
AluBI AGCT 5 cut(s) 91, 187, 566, 977, 1123
AluI AGCT 5 cut(s) 91, 187, 566, 977, 1123
Alw21I GWGCWC 1 cut(s) 1125
Alw26I GTCTC 2 cut(s) 236, 761
AlwI GGATC 4 cut(s) 927, 953, 1292, 1305
AlwNI CAGNNNCTG 1 cut(s) 1265
Ama87I CYCGRG 1 cut(s) 1124
AoxI GGCC 5 cut(s) 40, 654, 683, 1015, 1329
ApeKI GCWGC 3 cut(s) 137, 453, 974
ArsI GACNNNNNNTTYG 2 cut(s) 1148, 1180
Asp700I GAANNNNTTC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 1254
AspS9I GGNCC 4 cut(s) 612, 638, 655, 684
AsuC2I CCSGG 1 cut(s) 876
AsuHPI GGTGA 5 cut(s) 415, 605, 890, 1001, 1166
AvaI CYCGRG 1 cut(s) 1124
AvaII GGWCC 2 cut(s) 612, 638
BaeI ACNNNNGTAYC 2 cut(s) 622, 655
BalI TGGCCA 2 cut(s) 42, 1017
BamHI GGATCC 1 cut(s) 1297
BanII GRGCYC 3 cut(s) 678, 1125, 1137
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BauI CACGAG 1 cut(s) 851
Bbv12I GWGCWC 1 cut(s) 1125
BbvI GCAGC 3 cut(s) 124, 440, 986
BccI CCATC 3 cut(s) 383, 457, 584
BcgI CGANNNNNNTGC 2 cut(s) 1105, 1139
BciT130I CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
BciVI GTATCC 2 cut(s) 539, 1227
BclI TGATCA 1 cut(s) 666
BcnI CCSGG 1 cut(s) 876
BcoDI GTCTC 2 cut(s) 236, 761
BfrI CTTAAG 1 cut(s) 188
BfuI GTATCC 2 cut(s) 539, 1227
BglI GCCNNNNNGGC 1 cut(s) 682
BisI GCNGC 4 cut(s) 138, 454, 975, 1329
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 4 cut(s) 139, 455, 976, 1330
BmcAI AGTACT 2 cut(s) 254, 485
Bme1390I CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
Bme18I GGWCC 2 cut(s) 612, 638
BmeT110I CYCGRG 1 cut(s) 1124
BmgBI CACGTC 1 cut(s) 761
BmgT120I GGNCC 4 cut(s) 612, 638, 655, 684
BmiI GGNNCC 4 cut(s) 639, 1134, 1299, 1309
BmrFI CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
BmsI GCATC 3 cut(s) 262, 457, 834
BpmI CTGGAG 2 cut(s) 381, 845
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 2 cut(s) 671, 908
BpuMI CCSGG 1 cut(s) 876
BsaHI GRCGYC 1 cut(s) 720
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 8 cut(s) 321, 427, 951, 966, 990, 1012, 1191, 1311
BsaXI ACNNNNNCTCC 6 cut(s) 790, 820, 1124, 1154, 1299, 1329
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 652, 1226
Bse1I ACTGG 7 cut(s) 76, 316, 364, 474, 632, 775, 817
Bse3DI GCAATG 2 cut(s) 222, 1065
BseBI CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
BseDI CCNNGG 8 cut(s) 321, 427, 951, 966, 990, 1012, 1191, 1311
BseGI GGATG 3 cut(s) 394, 472, 524
BseLI CCNNNNNNNGG 3 cut(s) 581, 652, 1226
BseMI GCAATG 2 cut(s) 222, 1065
BseMII CTCAG 2 cut(s) 132, 186
BseNI ACTGG 7 cut(s) 76, 316, 364, 474, 632, 775, 817
BseXI GCAGC 3 cut(s) 124, 440, 986
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 5 cut(s) 42, 656, 685, 1017, 1331
BsiHKAI GWGCWC 1 cut(s) 1125
BsiHKCI CYCGRG 1 cut(s) 1124
BsiSI CCGG 1 cut(s) 876
BslFI GGGAC 2 cut(s) 462, 1259
BslI CCNNNNNNNGG 3 cut(s) 581, 652, 1226
BsmAI GTCTC 2 cut(s) 236, 761
BsmFI GGGAC 2 cut(s) 462, 1259
BsnI GGCC 5 cut(s) 42, 656, 685, 1017, 1331
Bso31I GGTCTC 1 cut(s) 236
BsoBI CYCGRG 1 cut(s) 1124
Bsp1286I GDGCHC 3 cut(s) 678, 1125, 1137
Bsp143I GATC 5 cut(s) 81, 666, 919, 958, 1297
Bsp1720I GCTNAGC 1 cut(s) 195
Bsp19I CCATGG 1 cut(s) 1191
BspACI CCGC 2 cut(s) 1305, 1328
BspANI GGCC 5 cut(s) 42, 656, 685, 1017, 1331
BspCNI CTCAG 2 cut(s) 133, 187
BspLI GGNNCC 4 cut(s) 639, 1134, 1299, 1309
BspPI GGATC 4 cut(s) 927, 953, 1292, 1305
BspQI GCTCTTC 1 cut(s) 876
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 2 cut(s) 222, 1065
BsrI ACTGG 7 cut(s) 76, 316, 364, 474, 632, 775, 817
BssECI CCNNGG 8 cut(s) 321, 427, 951, 966, 990, 1012, 1191, 1311
BssMI GATC 5 cut(s) 81, 666, 919, 958, 1297
BssNI GRCGYC 1 cut(s) 720
BssSI CACGAG 1 cut(s) 851
BssT1I CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
Bst2BI CACGAG 1 cut(s) 851
Bst2UI CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
Bst4CI ACNGT 4 cut(s) 265, 748, 1031, 1261
Bst6I CTCTTC 1 cut(s) 876
BstACI GRCGYC 1 cut(s) 720
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 3 cut(s) 141, 195, 384
BstDSI CCRYGG 1 cut(s) 1191
BstEII GGTNACC 1 cut(s) 1315
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 3 cut(s) 394, 472, 524
BstHHI GCGC 1 cut(s) 1254
BstKTI GATC 5 cut(s) 84, 669, 922, 961, 1300
BstMAI GTCTC 2 cut(s) 236, 761
BstMBI GATC 5 cut(s) 81, 666, 919, 958, 1297
BstMWI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
BstNI CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
BstNSI RCATGY 2 cut(s) 410, 1252
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
BstV1I GCAGC 3 cut(s) 124, 440, 986
BstX2I RGATCY 3 cut(s) 919, 958, 1297
BstXI CCANNNNNNTGG 1 cut(s) 1192
BstYI RGATCY 3 cut(s) 919, 958, 1297
BsuI GTATCC 2 cut(s) 539, 1227
BsuRI GGCC 5 cut(s) 42, 656, 685, 1017, 1331
BtgI CCRYGG 1 cut(s) 1191
BtrI CACGTC 1 cut(s) 761
BtsCI GGATG 3 cut(s) 394, 472, 524
BtsIMutI CAGTG 5 cut(s) 69, 261, 357, 810, 822
Cac8I GCNNGC 1 cut(s) 408
CaiI CAGNNNCTG 1 cut(s) 1265
CfoI GCGC 1 cut(s) 1254
Cfr13I GGNCC 4 cut(s) 612, 638, 655, 684
CseI GACGC 1 cut(s) 1166
Csp6I GTAC 3 cut(s) 253, 484, 631
CviAII CATG 7 cut(s) 44, 304, 407, 601, 664, 1192, 1249
CviQI GTAC 3 cut(s) 253, 484, 631
DdeI CTNAG 3 cut(s) 141, 195, 384
DpnI GATC 5 cut(s) 83, 668, 921, 960, 1299
DpnII GATC 5 cut(s) 81, 666, 919, 958, 1297
EaeI YGGCCR 3 cut(s) 40, 1015, 1329
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
Ecl136II GAGCTC 1 cut(s) 1123
Eco130I CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
Eco24I GRGCYC 3 cut(s) 678, 1125, 1137
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 2 cut(s) 612, 638
Eco53kI GAGCTC 1 cut(s) 1123
Eco57I CTGAAG 3 cut(s) 554, 698, 846
Eco88I CYCGRG 1 cut(s) 1124
Eco91I GGTNACC 1 cut(s) 1315
EcoICRI GAGCTC 1 cut(s) 1123
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO109I RGGNCCY 2 cut(s) 655, 684
EcoO65I GGTNACC 1 cut(s) 1315
EcoRII CCWGG 6 cut(s) 277, 321, 784, 861, 990, 1012
EcoT14I CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
EcoT38I GRGCYC 3 cut(s) 678, 1125, 1137
ErhI CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
FaeI CATG 7 cut(s) 47, 307, 410, 604, 667, 1195, 1252
FaqI GGGAC 2 cut(s) 462, 1259
FatI CATG 7 cut(s) 43, 303, 406, 600, 663, 1191, 1248
FbaI TGATCA 1 cut(s) 666
FblI GTMKAC 2 cut(s) 62, 723
Fnu4HI GCNGC 4 cut(s) 138, 454, 975, 1329
FokI GGATG 3 cut(s) 401, 479, 511
FriOI GRGCYC 3 cut(s) 678, 1125, 1137
Fsp4HI GCNGC 4 cut(s) 138, 454, 975, 1329
GlaI GCGC 1 cut(s) 1253
GluI GCNGC 4 cut(s) 138, 454, 975, 1329
GsuI CTGGAG 2 cut(s) 381, 845
HaeIII GGCC 5 cut(s) 42, 656, 685, 1017, 1331
HapII CCGG 1 cut(s) 876
HgaI GACGC 1 cut(s) 1166
HhaI GCGC 1 cut(s) 1254
Hin1I GRCGYC 1 cut(s) 720
Hin1II CATG 7 cut(s) 47, 307, 410, 604, 667, 1195, 1252
Hin6I GCGC 1 cut(s) 1252
HinP1I GCGC 1 cut(s) 1252
HincII GTYRAC 2 cut(s) 49, 63
HindII GTYRAC 2 cut(s) 49, 63
HindIII AAGCTT 2 cut(s) 89, 185
HinfI GANTC 5 cut(s) 50, 350, 1006, 1177, 1272
HpaII CCGG 1 cut(s) 876
HphI GGTGA 5 cut(s) 415, 605, 890, 1001, 1166
Hpy166II GTNNAC 4 cut(s) 49, 63, 615, 724
Hpy188I TCNGA 5 cut(s) 396, 534, 691, 704, 1213
Hpy8I GTNNAC 4 cut(s) 49, 63, 615, 724
HpyAV CCTTC 6 cut(s) 124, 484, 508, 656, 1123, 1288
HpyCH4III ACNGT 4 cut(s) 265, 748, 1031, 1261
HpyCH4IV ACGT 5 cut(s) 720, 760, 897, 906, 1108
HpyCH4V TGCA 4 cut(s) 261, 456, 847, 911
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
HpyF3I CTNAG 3 cut(s) 141, 195, 384
HpySE526I ACGT 5 cut(s) 720, 760, 897, 906, 1108
Hsp92I GRCGYC 1 cut(s) 720
Hsp92II CATG 7 cut(s) 47, 307, 410, 604, 667, 1195, 1252
HspAI GCGC 1 cut(s) 1252
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 5 cut(s) 81, 666, 919, 958, 1297
LguI GCTCTTC 1 cut(s) 876
LmnI GCTCC 6 cut(s) 280, 811, 1042, 1120, 1132, 1307
Lsp1109I GCAGC 3 cut(s) 124, 440, 986
LweI GCATC 3 cut(s) 262, 457, 834
MaeII ACGT 5 cut(s) 720, 760, 897, 906, 1108
MaeIII GTNAC 6 cut(s) 287, 593, 796, 878, 1154, 1315
MalI GATC 5 cut(s) 83, 668, 921, 960, 1299
MboI GATC 5 cut(s) 81, 666, 919, 958, 1297
MboII GAAGA 6 cut(s) 547, 550, 863, 968, 1159, 1303
MflI RGATCY 3 cut(s) 919, 958, 1297
MhlI GDGCHC 3 cut(s) 678, 1125, 1137
MlsI TGGCCA 2 cut(s) 42, 1017
MluNI TGGCCA 2 cut(s) 42, 1017
MlyI GAGTC 3 cut(s) 44, 344, 1281
MmeI TCCRAC 4 cut(s) 665, 714, 727, 736
Mox20I TGGCCA 2 cut(s) 42, 1017
MroXI GAANNNNTTC 1 cut(s) 15
MscI TGGCCA 2 cut(s) 42, 1017
MseI TTAA 2 cut(s) 189, 1170
MslI CAYNNNNRTG 1 cut(s) 1001
Msp20I TGGCCA 2 cut(s) 42, 1017
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 1 cut(s) 876
MspR9I CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
MvaI CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
MwoI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
NciI CCSGG 1 cut(s) 876
NcoI CCATGG 1 cut(s) 1191
NdeII GATC 5 cut(s) 81, 666, 919, 958, 1297
NlaIII CATG 7 cut(s) 47, 307, 410, 604, 667, 1195, 1252
NlaIV GGNNCC 4 cut(s) 639, 1134, 1299, 1309
NmuCI GTSAC 5 cut(s) 287, 593, 796, 878, 1154
NspI RCATGY 2 cut(s) 410, 1252
PaeI GCATGC 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 1124
PciSI GCTCTTC 1 cut(s) 876
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 1006, 1177
PfoI TCCNGGA 2 cut(s) 277, 861
PkrI GCNGC 4 cut(s) 139, 455, 976, 1330
PleI GAGTC 3 cut(s) 44, 344, 1280
PpsI GAGTC 3 cut(s) 44, 344, 1280
Psp124BI GAGCTC 1 cut(s) 1125
Psp1406I AACGTT 1 cut(s) 906
Psp6I CCWGG 6 cut(s) 277, 321, 784, 861, 990, 1012
PspEI GGTNACC 1 cut(s) 1315
PspGI CCWGG 6 cut(s) 277, 321, 784, 861, 990, 1012
PspN4I GGNNCC 4 cut(s) 639, 1134, 1299, 1309
PspPI GGNCC 4 cut(s) 612, 638, 655, 684
PstNI CAGNNNCTG 1 cut(s) 1265
PsuI RGATCY 3 cut(s) 919, 958, 1297
RsaI GTAC 3 cut(s) 254, 485, 632
RsaNI GTAC 3 cut(s) 253, 484, 631
RseI CAYNNNNRTG 1 cut(s) 1001
SacI GAGCTC 1 cut(s) 1125
SalI GTCGAC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 2 cut(s) 189, 1170
SatI GCNGC 4 cut(s) 138, 454, 975, 1329
Sau3AI GATC 5 cut(s) 81, 666, 919, 958, 1297
Sau96I GGNCC 4 cut(s) 612, 638, 655, 684
ScaI AGTACT 2 cut(s) 254, 485
SchI GAGTC 3 cut(s) 44, 344, 1281
ScrFI CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
SduI GDGCHC 3 cut(s) 678, 1125, 1137
SfaNI GCATC 3 cut(s) 262, 457, 834
Sfr274I CTCGAG 1 cut(s) 1124
SinI GGWCC 2 cut(s) 612, 638
SlaI CTCGAG 1 cut(s) 1124
SmiMI CAYNNNNRTG 1 cut(s) 1001
SmlI CTYRAG 4 cut(s) 188, 650, 923, 1124
SmoI CTYRAG 4 cut(s) 188, 650, 923, 1124
SphI GCATGC 1 cut(s) 410
SsiI CCGC 2 cut(s) 1305, 1328
SstI GAGCTC 1 cut(s) 1125
StyD4I CCNGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
StyI CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
TaaI ACNGT 4 cut(s) 265, 748, 1031, 1261
TaiI ACGT 5 cut(s) 723, 763, 900, 909, 1111
TaqI TCGA 5 cut(s) 62, 126, 488, 624, 1125
TatI WGTACW 2 cut(s) 252, 483
TauI GCSGC 1 cut(s) 1331
TfiI GAWTC 2 cut(s) 1006, 1177
Tru1I TTAA 2 cut(s) 189, 1170
Tru9I TTAA 2 cut(s) 189, 1170
TscAI CASTG 5 cut(s) 76, 268, 364, 817, 829
TseFI GTSAC 5 cut(s) 287, 593, 796, 878, 1154
TseI GCWGC 3 cut(s) 137, 453, 974
Tsp45I GTSAC 5 cut(s) 287, 593, 796, 878, 1154
TspDTI ATGAA 6 cut(s) 8, 147, 161, 283, 519, 1019
TspRI CASTG 5 cut(s) 76, 268, 364, 817, 829
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 612, 638
XagI CCTNNNNNAGG 1 cut(s) 650
XceI RCATGY 2 cut(s) 410, 1252
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 1189
XhoI CTCGAG 1 cut(s) 1124
XmiI GTMKAC 2 cut(s) 62, 723
XmnI GAANNNNTTC 1 cut(s) 15
ZraI GACGTC 1 cut(s) 721
ZrmI AGTACT 2 cut(s) 254, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.