Rw5G037140

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000682
Physical Location & Seq
Forward (+)
65459123 .. 65462402
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037140.1

Sequence Viewer

Length: 1344 bp
ATGGGTAAGGAAAAGTTCCATATCAACATCGTGGTCATTGGCCACGTCGACTCTGGCAAGTCGACCACCACAGGTCACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATTGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAAAGGTCATTCAAGTATGCCTGGGTGTTGGACAAGCTCAAGGCTGAGCGTGAGCGTGGTATCACCATTGATATTGCCTTGTGGAAGTTTGAGACCACCAAGTACTACTGCACTGTCATTGATGCTCCCGGACATCGTGATTTCATCAAGAACATGATTACTGGTACCTCCCAGGCTGATTGTGCCGTCCTCATCATCGACTCCACCACTGGTGGTTTTGAAGCTGGTATCTCCAAGGATGGTCAGACCCGTGAGCACGCTCTGCTTGCTTTCACACTTGGTGTCAAGCAGATGATTTGCTGCTGTAACAAGATGGATGCCACCACTCCCAAGTACTCAAAGGCAAGGTATGATGAAATCGTGAAGGAAGTCTCTTCCTACCTGAAGAAGGTTGGGTACAACCCAGACAAAATTGCCTTTGTTCCCATCTCTGGATTCGAGGGTGACAACATGATTGAGAGGTCCACCAACCTCGACTGGTACAAGGGCCCTACCCTCCTTGAGGCACTTGACCAAATCAATGAGCCCAAGAGGCCCTCAGACAAGCCCCTCCGTCTCCCACTTCAGGATGTGTACAAGATTGGTGGCATTGGAACTGTGCCTGTGGGACGTGTTGAGACTGGTGTCATCAAGCCTGGTATGGTTGTGACTTTTGGCCCCACTGGACTGACTACTGAAGTTAAGTCTGTGGAGATGCACCACGAAGCTCTCCTGGAGGCTCTTCCTGGTGACAATGTTGGGTTCAATGTGAAGAATGTTGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCATCCAACTCCAAGGATGACCCTGCCAAGGAAGCTGCCAACTTCACCTCACAGGTCATCATCATGAACCACCCTGGCCAGATTGGAAATGGATATGCTCCAGTGCTCGACTGCCACACCTCCCACATTGCTGTTAAGTTTGGTGAGATTTTGACCAAGATTGACAGGCGATCTGGTAAGGAGATTGAGAAGGAGCCCAAGTTCTTGAAGAATGGTGATGCTGGTATGGTTAAGATGCTTCCCACCAAGCCCATGGTTGTTGAGACCTTCTCTGAGTACCCACCTCTTGGTCGTTTTGCTGTGAGGGATATGCGTCAGACAGTTGCTGTTGGAGTCATCAAGAGTGTTGAGAAGAAGGATCCATCAGGTGCCAAGGTGACCAAGTCTGCTGCTAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

447

Amino Acids

49.3

Weight (kDa)

9.15

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 223 9.9e-58 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 248 - 313 1.3e-16 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 322 - 429 1e-26 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 327 - 427 7.5e-37 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 314
AccB1I GGYRCC 2 cut(s) 314, 1307
AccB7I CCANNNNNTGG 1 cut(s) 1226
AccI GTMKAC 2 cut(s) 48, 62
AclWI GGATC 3 cut(s) 927, 1292, 1305
AcoI YGGCCR 2 cut(s) 40, 1015
AcuI CTGAAG 3 cut(s) 554, 698, 846
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 7 cut(s) 254, 316, 485, 548, 632, 725, 1217
AfiI CCNNNNNNNGG 6 cut(s) 538, 581, 652, 715, 967, 1226
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 4 cut(s) 163, 371, 895, 1147
AjiI CACGTC 2 cut(s) 46, 761
AjnI CCWGG 6 cut(s) 170, 321, 784, 861, 874, 1012
AloI GAACNNNNNNTCC 2 cut(s) 1124, 1156
AluBI AGCT 5 cut(s) 91, 187, 374, 857, 974
AluI AGCT 5 cut(s) 91, 187, 374, 857, 974
Alw21I GWGCWC 2 cut(s) 408, 1047
Alw26I GTCTC 5 cut(s) 236, 526, 710, 761, 1196
AlwI GGATC 3 cut(s) 927, 1292, 1305
AlwNI CAGNNNCTG 1 cut(s) 1265
AoxI GGCC 5 cut(s) 40, 637, 683, 805, 1015
ApaI GGGCCC 1 cut(s) 641
ApeKI GCWGC 4 cut(s) 137, 450, 974, 1328
Asp718I GGTACC 1 cut(s) 314
AspS9I GGNCC 5 cut(s) 612, 637, 638, 684, 806
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 7 cut(s) 205, 605, 890, 976, 1094, 1166, 1327
AvaII GGWCC 1 cut(s) 612
BaeGI GKGCMC 1 cut(s) 641
BalI TGGCCA 2 cut(s) 42, 1017
BamHI GGATCC 1 cut(s) 1297
BanI GGYRCC 2 cut(s) 314, 1307
BanII GRGCYC 3 cut(s) 641, 678, 1137
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
Bbv12I GWGCWC 2 cut(s) 408, 1047
BbvI GCAGC 4 cut(s) 124, 437, 961, 1315
BccI CCATC 4 cut(s) 383, 457, 584, 1309
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 6 cut(s) 172, 323, 786, 863, 876, 1014
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 5 cut(s) 236, 526, 710, 761, 1196
BglI GCCNNNNNGGC 1 cut(s) 682
BisI GCNGC 4 cut(s) 138, 451, 975, 1329
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 4 cut(s) 139, 452, 976, 1330
BmcAI AGTACT 2 cut(s) 254, 485
Bme1390I CCNGG 7 cut(s) 172, 279, 323, 786, 863, 876, 1014
Bme18I GGWCC 1 cut(s) 612
BmgBI CACGTC 2 cut(s) 46, 761
BmgT120I GGNCC 5 cut(s) 612, 637, 638, 684, 806
BmiI GGNNCC 6 cut(s) 316, 639, 808, 1134, 1299, 1309
BmrFI CCNGG 7 cut(s) 172, 279, 323, 786, 863, 876, 1014
BmsI GCATC 6 cut(s) 262, 457, 834, 950, 1147, 1164
BoxI GACNNNNGTC 1 cut(s) 773
BplI GAGNNNNNCTC 2 cut(s) 1193, 1225
BpmI CTGGAG 2 cut(s) 884, 1023
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 3 cut(s) 173, 671, 908
BpuMI CCSGG 1 cut(s) 279
BsaI GGTCTC 2 cut(s) 236, 1196
BsaJI CCNNGG 8 cut(s) 171, 321, 384, 951, 966, 1012, 1191, 1311
BsaXI ACNNNNNCTCC 2 cut(s) 1124, 1154
Bsc4I CCNNNNNNNGG 6 cut(s) 538, 581, 652, 715, 967, 1226
Bse1I ACTGG 6 cut(s) 316, 364, 632, 775, 817, 1040
Bse3DI GCAATG 1 cut(s) 1065
BseBI CCWGG 6 cut(s) 172, 323, 786, 863, 876, 1014
BseDI CCNNGG 8 cut(s) 171, 321, 384, 951, 966, 1012, 1191, 1311
BseGI GGATG 5 cut(s) 394, 472, 724, 941, 961
BseLI CCNNNNNNNGG 6 cut(s) 538, 581, 652, 715, 967, 1226
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 4 cut(s) 132, 186, 702, 1203
BseNI ACTGG 6 cut(s) 316, 364, 632, 775, 817, 1040
BseSI GKGCMC 1 cut(s) 641
BseXI GCAGC 4 cut(s) 124, 437, 961, 1315
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 5 cut(s) 42, 639, 685, 807, 1017
BshNI GGYRCC 2 cut(s) 314, 1307
BsiHKAI GWGCWC 2 cut(s) 408, 1047
BsiSI CCGG 1 cut(s) 279
BslFI GGGAC 1 cut(s) 771
BslI CCNNNNNNNGG 6 cut(s) 538, 581, 652, 715, 967, 1226
BsmAI GTCTC 5 cut(s) 236, 526, 710, 761, 1196
BsmBI CGTCTC 1 cut(s) 710
BsmFI GGGAC 1 cut(s) 771
BsnI GGCC 5 cut(s) 42, 639, 685, 807, 1017
Bso31I GGTCTC 2 cut(s) 236, 1196
Bsp120I GGGCCC 1 cut(s) 637
Bsp1286I GDGCHC 5 cut(s) 408, 641, 678, 1047, 1137
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 4 cut(s) 81, 919, 1109, 1297
Bsp1720I GCTNAGC 1 cut(s) 195
Bsp19I CCATGG 1 cut(s) 1191
BspANI GGCC 5 cut(s) 42, 639, 685, 807, 1017
BspCNI CTCAG 4 cut(s) 133, 187, 701, 1204
BspHI TCATGA 1 cut(s) 1002
BspLI GGNNCC 6 cut(s) 316, 639, 808, 1134, 1299, 1309
BspPI GGATC 3 cut(s) 927, 1292, 1305
BspQI GCTCTTC 1 cut(s) 876
BspT107I GGYRCC 2 cut(s) 314, 1307
BspTNI GGTCTC 2 cut(s) 236, 1196
BsrDI GCAATG 1 cut(s) 1065
BsrGI TGTACA 1 cut(s) 723
BsrI ACTGG 6 cut(s) 316, 364, 632, 775, 817, 1040
BssECI CCNNGG 8 cut(s) 171, 321, 384, 951, 966, 1012, 1191, 1311
BssMI GATC 4 cut(s) 81, 919, 1109, 1297
BssT1I CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
Bst2UI CCWGG 6 cut(s) 172, 323, 786, 863, 876, 1014
Bst4CI ACNGT 3 cut(s) 265, 748, 1261
Bst6I CTCTTC 2 cut(s) 529, 876
BstAPI GCANNNNNTGC 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 723
BstC8I GCNNGC 2 cut(s) 408, 417
BstDEI CTNAG 5 cut(s) 141, 195, 688, 1212, 1332
BstDSI CCRYGG 1 cut(s) 1191
BstEII GGTNACC 1 cut(s) 1315
BstENI CCTNNNNNAGG 2 cut(s) 536, 650
BstF5I GGATG 5 cut(s) 394, 472, 724, 941, 961
BstKTI GATC 4 cut(s) 84, 922, 1112, 1300
BstMAI GTCTC 5 cut(s) 236, 526, 710, 761, 1196
BstMBI GATC 4 cut(s) 81, 919, 1109, 1297
BstMWI GCNNNNNNNGC 5 cut(s) 332, 412, 416, 682, 971
BstNI CCWGG 6 cut(s) 172, 323, 786, 863, 876, 1014
BstPAI GACNNNNGTC 1 cut(s) 773
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 7 cut(s) 170, 277, 321, 784, 861, 874, 1012
BstSLI GKGCMC 1 cut(s) 641
BstV1I GCAGC 4 cut(s) 124, 437, 961, 1315
BstX2I RGATCY 2 cut(s) 919, 1297
BstXI CCANNNNNNTGG 1 cut(s) 1192
BstYI RGATCY 2 cut(s) 919, 1297
BsuRI GGCC 5 cut(s) 42, 639, 685, 807, 1017
BtgI CCRYGG 1 cut(s) 1191
BtrI CACGTC 2 cut(s) 46, 761
BtsCI GGATG 5 cut(s) 394, 472, 724, 941, 961
BtsIMutI CAGTG 4 cut(s) 261, 357, 810, 1047
Cac8I GCNNGC 2 cut(s) 408, 417
CaiI CAGNNNCTG 1 cut(s) 1265
CciI TCATGA 1 cut(s) 1002
Cfr13I GGNCC 5 cut(s) 612, 637, 638, 684, 806
CseI GACGC 1 cut(s) 1241
Csp6I GTAC 7 cut(s) 253, 315, 484, 547, 631, 724, 1216
CspCI CAANNNNNGTGG 4 cut(s) 76, 111, 715, 750
CviAII CATG 4 cut(s) 304, 601, 1003, 1192
CviQI GTAC 7 cut(s) 253, 315, 484, 547, 631, 724, 1216
DdeI CTNAG 5 cut(s) 141, 195, 688, 1212, 1332
DpnI GATC 4 cut(s) 83, 921, 1111, 1299
DpnII GATC 4 cut(s) 81, 919, 1109, 1297
DraIII CACNNNGTG 1 cut(s) 431
EaeI YGGCCR 2 cut(s) 40, 1015
Eam1104I CTCTTC 2 cut(s) 529, 876
EarI CTCTTC 2 cut(s) 529, 876
Eco130I CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
Eco24I GRGCYC 3 cut(s) 641, 678, 1137
Eco31I GGTCTC 2 cut(s) 236, 1196
Eco47I GGWCC 1 cut(s) 612
Eco57I CTGAAG 3 cut(s) 554, 698, 846
Eco91I GGTNACC 1 cut(s) 1315
EcoNI CCTNNNNNAGG 2 cut(s) 536, 650
EcoO109I RGGNCCY 3 cut(s) 637, 638, 684
EcoO65I GGTNACC 1 cut(s) 1315
EcoRII CCWGG 6 cut(s) 170, 321, 784, 861, 874, 1012
EcoT14I CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
EcoT38I GRGCYC 3 cut(s) 641, 678, 1137
ErhI CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 4 cut(s) 307, 604, 1006, 1195
FaqI GGGAC 1 cut(s) 771
FatI CATG 4 cut(s) 303, 600, 1002, 1191
FblI GTMKAC 2 cut(s) 48, 62
Fnu4HI GCNGC 4 cut(s) 138, 451, 975, 1329
FokI GGATG 5 cut(s) 401, 479, 731, 928, 968
FriOI GRGCYC 3 cut(s) 641, 678, 1137
Fsp4HI GCNGC 4 cut(s) 138, 451, 975, 1329
GluI GCNGC 4 cut(s) 138, 451, 975, 1329
GsuI CTGGAG 2 cut(s) 884, 1023
HaeIII GGCC 5 cut(s) 42, 639, 685, 807, 1017
HapII CCGG 1 cut(s) 279
HgaI GACGC 1 cut(s) 1241
Hin1II CATG 4 cut(s) 307, 604, 1006, 1195
HincII GTYRAC 2 cut(s) 49, 63
HindII GTYRAC 2 cut(s) 49, 63
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 4 cut(s) 50, 350, 585, 1272
HpaII CCGG 1 cut(s) 279
HphI GGTGA 7 cut(s) 205, 605, 890, 976, 1094, 1166, 1327
Hpy166II GTNNAC 4 cut(s) 49, 63, 615, 724
Hpy188I TCNGA 4 cut(s) 396, 691, 1213, 1257
Hpy188III TCNNGA 9 cut(s) 127, 287, 298, 511, 582, 716, 1003, 1144, 1279
Hpy8I GTNNAC 4 cut(s) 49, 63, 615, 724
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 6 cut(s) 124, 508, 532, 1123, 1216, 1288
HpyCH4III ACNGT 3 cut(s) 265, 748, 1261
HpyCH4IV ACGT 2 cut(s) 45, 760
HpyCH4V TGCA 3 cut(s) 261, 847, 941
HpyF10VI GCNNNNNNNGC 5 cut(s) 332, 412, 416, 682, 971
HpyF3I CTNAG 5 cut(s) 141, 195, 688, 1212, 1332
HpySE526I ACGT 2 cut(s) 45, 760
Hsp92II CATG 4 cut(s) 307, 604, 1006, 1195
KpnI GGTACC 1 cut(s) 318
Kzo9I GATC 4 cut(s) 81, 919, 1109, 1297
LguI GCTCTTC 1 cut(s) 876
LmnI GCTCC 3 cut(s) 280, 1042, 1132
Lsp1109I GCAGC 4 cut(s) 124, 437, 961, 1315
LweI GCATC 6 cut(s) 262, 457, 834, 950, 1147, 1164
MaeII ACGT 2 cut(s) 45, 760
MaeIII GTNAC 6 cut(s) 74, 455, 593, 796, 878, 1315
MalI GATC 4 cut(s) 83, 921, 1111, 1299
MboI GATC 4 cut(s) 81, 919, 1109, 1297
MboII GAAGA 6 cut(s) 516, 547, 863, 913, 1159, 1303
MflI RGATCY 2 cut(s) 919, 1297
MhlI GDGCHC 5 cut(s) 408, 641, 678, 1047, 1137
MlsI TGGCCA 2 cut(s) 42, 1017
MluCI AATT 1 cut(s) 561
MluNI TGGCCA 2 cut(s) 42, 1017
MlyI GAGTC 3 cut(s) 44, 344, 1281
MmeI TCCRAC 3 cut(s) 159, 969, 1249
Mox20I TGGCCA 2 cut(s) 42, 1017
MscI TGGCCA 2 cut(s) 42, 1017
MseI TTAA 3 cut(s) 831, 1074, 1170
MslI CAYNNNNRTG 1 cut(s) 1001
Msp20I TGGCCA 2 cut(s) 42, 1017
MspI CCGG 1 cut(s) 279
MspR9I CCNGG 7 cut(s) 172, 279, 323, 786, 863, 876, 1014
MvaI CCWGG 6 cut(s) 172, 323, 786, 863, 876, 1014
MwoI GCNNNNNNNGC 5 cut(s) 332, 412, 416, 682, 971
NciI CCSGG 1 cut(s) 279
NcoI CCATGG 1 cut(s) 1191
NdeII GATC 4 cut(s) 81, 919, 1109, 1297
NlaIII CATG 4 cut(s) 307, 604, 1006, 1195
NlaIV GGNNCC 6 cut(s) 316, 639, 808, 1134, 1299, 1309
NmuCI GTSAC 5 cut(s) 74, 593, 796, 878, 1315
PagI TCATGA 1 cut(s) 1002
PciSI GCTCTTC 1 cut(s) 876
PfeI GAWTC 1 cut(s) 585
PflFI GACNNNGTC 1 cut(s) 1321
PflMI CCANNNNNTGG 1 cut(s) 1226
PfoI TCCNGGA 2 cut(s) 277, 861
PkrI GCNGC 4 cut(s) 139, 452, 976, 1330
PleI GAGTC 3 cut(s) 44, 344, 1280
PpsI GAGTC 3 cut(s) 44, 344, 1280
PshAI GACNNNNGTC 1 cut(s) 773
Psp6I CCWGG 6 cut(s) 170, 321, 784, 861, 874, 1012
PspEI GGTNACC 1 cut(s) 1315
PspGI CCWGG 6 cut(s) 170, 321, 784, 861, 874, 1012
PspN4I GGNNCC 6 cut(s) 316, 639, 808, 1134, 1299, 1309
PspOMI GGGCCC 1 cut(s) 637
PspPI GGNCC 5 cut(s) 612, 637, 638, 684, 806
PstNI CAGNNNCTG 1 cut(s) 1265
PsuI RGATCY 2 cut(s) 919, 1297
PsyI GACNNNGTC 1 cut(s) 1321
RsaI GTAC 7 cut(s) 254, 316, 485, 548, 632, 725, 1217
RsaNI GTAC 7 cut(s) 253, 315, 484, 547, 631, 724, 1216
RseI CAYNNNNRTG 1 cut(s) 1001
SalI GTCGAC 2 cut(s) 47, 61
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 3 cut(s) 831, 1074, 1170
SatI GCNGC 4 cut(s) 138, 451, 975, 1329
Sau3AI GATC 4 cut(s) 81, 919, 1109, 1297
Sau96I GGNCC 5 cut(s) 612, 637, 638, 684, 806
ScaI AGTACT 2 cut(s) 254, 485
SchI GAGTC 3 cut(s) 44, 344, 1281
ScrFI CCNGG 7 cut(s) 172, 279, 323, 786, 863, 876, 1014
SduI GDGCHC 5 cut(s) 408, 641, 678, 1047, 1137
SfaNI GCATC 6 cut(s) 262, 457, 834, 950, 1147, 1164
SinI GGWCC 1 cut(s) 612
SmiMI CAYNNNNRTG 1 cut(s) 1001
SmlI CTYRAG 3 cut(s) 188, 650, 923
SmoI CTYRAG 3 cut(s) 188, 650, 923
Sse9I AATT 1 cut(s) 561
StyD4I CCNGG 7 cut(s) 170, 277, 321, 784, 861, 874, 1012
StyI CCWWGG 5 cut(s) 384, 951, 966, 1191, 1311
TaaI ACNGT 3 cut(s) 265, 748, 1261
TaiI ACGT 2 cut(s) 48, 763
TaqI TCGA 7 cut(s) 48, 62, 126, 348, 588, 624, 1047
TasI AATT 1 cut(s) 561
TatI WGTACW 3 cut(s) 252, 483, 723
TfiI GAWTC 1 cut(s) 585
Tru1I TTAA 3 cut(s) 831, 1074, 1170
Tru9I TTAA 3 cut(s) 831, 1074, 1170
TscAI CASTG 4 cut(s) 268, 364, 817, 1047
TseFI GTSAC 5 cut(s) 74, 593, 796, 878, 1315
TseI GCWGC 4 cut(s) 137, 450, 974, 1328
Tsp45I GTSAC 5 cut(s) 74, 593, 796, 878, 1315
TspDTI ATGAA 4 cut(s) 161, 283, 519, 1019
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 4 cut(s) 268, 364, 817, 1047
Tth111I GACNNNGTC 1 cut(s) 1321
Van91I CCANNNNNTGG 1 cut(s) 1226
VpaK11BI GGWCC 1 cut(s) 612
XagI CCTNNNNNAGG 2 cut(s) 536, 650
XcmI CCANNNNNNNNNTGG 3 cut(s) 50, 1025, 1189
XmiI GTMKAC 2 cut(s) 48, 62
ZrmI AGTACT 2 cut(s) 254, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.