This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| GTP_EFTU | PF00009 | 7 - 223 | 1e-57 | Elongation factor Tu GTP binding domain |
| GTP_EFTU_D2 | PF03144 | 248 - 313 | 2.9e-17 | Elongation factor Tu domain 2 |
| GTP_EFTU_D3 | PF03143 | 322 - 368 | 7.8e-10 | Elongation factor Tu C-terminal domain |
| GTP-eEF1A_C | PF22594 | 327 - 373 | 7e-11 | GTP-eEF1A C-terminal domain-like |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 743 |
| AccB1I | GGYRCC | 1 cut(s) | 743 |
| AccBSI | CCGCTC | 1 cut(s) | 989 |
| AccI | GTMKAC | 2 cut(s) | 48, 62 |
| AciI | CCGC | 2 cut(s) | 963, 987 |
| AclI | AACGTT | 1 cut(s) | 897 |
| AclWI | GGATC | 4 cut(s) | 661, 927, 953, 1115 |
| AcoI | YGGCCR | 2 cut(s) | 40, 1015 |
| AcuI | CTGAAG | 1 cut(s) | 698 |
| AfaI | GTAC | 5 cut(s) | 254, 485, 632, 725, 745 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 581, 582, 652 |
| AflII | CTTAAG | 1 cut(s) | 188 |
| AflIII | ACRYGT | 1 cut(s) | 760 |
| AgsI | TTSAA | 3 cut(s) | 104, 371, 895 |
| AjiI | CACGTC | 1 cut(s) | 761 |
| AjnI | CCWGG | 4 cut(s) | 170, 277, 784, 874 |
| AluBI | AGCT | 5 cut(s) | 91, 187, 857, 1087, 1125 |
| AluI | AGCT | 5 cut(s) | 91, 187, 857, 1087, 1125 |
| Alw21I | GWGCWC | 1 cut(s) | 1089 |
| Alw26I | GTCTC | 2 cut(s) | 236, 761 |
| AlwI | GGATC | 4 cut(s) | 661, 927, 953, 1115 |
| Ama87I | CYCGRG | 1 cut(s) | 650 |
| AoxI | GGCC | 5 cut(s) | 40, 683, 805, 867, 1015 |
| ApeKI | GCWGC | 3 cut(s) | 450, 453, 974 |
| Asp718I | GGTACC | 1 cut(s) | 743 |
| AspS9I | GGNCC | 5 cut(s) | 612, 638, 684, 806, 868 |
| AsuC2I | CCSGG | 1 cut(s) | 1014 |
| AsuHPI | GGTGA | 3 cut(s) | 605, 808, 890 |
| AvaI | CYCGRG | 1 cut(s) | 650 |
| AvaII | GGWCC | 2 cut(s) | 612, 638 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 622, 655 |
| BalI | TGGCCA | 1 cut(s) | 42 |
| BanI | GGYRCC | 1 cut(s) | 743 |
| BanII | GRGCYC | 3 cut(s) | 678, 1089, 1139 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 530, 562 |
| BauI | CACGAG | 1 cut(s) | 671 |
| Bbv12I | GWGCWC | 1 cut(s) | 1089 |
| BbvI | GCAGC | 3 cut(s) | 437, 440, 961 |
| BccI | CCATC | 2 cut(s) | 383, 457 |
| BciT130I | CCWGG | 4 cut(s) | 172, 279, 786, 876 |
| BcnI | CCSGG | 1 cut(s) | 1014 |
| BcoDI | GTCTC | 2 cut(s) | 236, 761 |
| BfrI | CTTAAG | 1 cut(s) | 188 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 682, 703 |
| BisI | GCNGC | 3 cut(s) | 451, 454, 975 |
| BlpI | GCTNAGC | 2 cut(s) | 195, 1083 |
| BlsI | GCNGC | 3 cut(s) | 452, 455, 976 |
| BmcAI | AGTACT | 2 cut(s) | 254, 485 |
| Bme1390I | CCNGG | 5 cut(s) | 172, 279, 786, 876, 1014 |
| Bme18I | GGWCC | 2 cut(s) | 612, 638 |
| BmeT110I | CYCGRG | 1 cut(s) | 650 |
| BmgBI | CACGTC | 1 cut(s) | 761 |
| BmgT120I | GGNCC | 5 cut(s) | 612, 638, 684, 806, 868 |
| BmiI | GGNNCC | 5 cut(s) | 316, 639, 640, 745, 1136 |
| BmrFI | CCNGG | 5 cut(s) | 172, 279, 786, 876, 1014 |
| BmsI | GCATC | 3 cut(s) | 262, 457, 834 |
| BoxI | GACNNNNGTC | 1 cut(s) | 773 |
| BpmI | CTGGAG | 2 cut(s) | 381, 1023 |
| Bpu10I | CCTNAGC | 1 cut(s) | 990 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 195, 1083 |
| BpuEI | CTTGAG | 4 cut(s) | 472, 518, 908, 1147 |
| BpuMI | CCSGG | 1 cut(s) | 1014 |
| Bsa29I | ATCGAT | 1 cut(s) | 1110 |
| BsaI | GGTCTC | 1 cut(s) | 236 |
| BsaJI | CCNNGG | 4 cut(s) | 171, 384, 951, 1012 |
| BsaXI | ACNNNNNCTCC | 1 cut(s) | 1126 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 581, 582, 652 |
| Bse118I | RCCGGY | 1 cut(s) | 374 |
| Bse1I | ACTGG | 7 cut(s) | 76, 316, 364, 632, 775, 817, 1040 |
| Bse3DI | GCAATG | 2 cut(s) | 222, 1065 |
| BseBI | CCWGG | 4 cut(s) | 172, 279, 786, 876 |
| BseCI | ATCGAT | 1 cut(s) | 1110 |
| BseDI | CCNNGG | 4 cut(s) | 171, 384, 951, 1012 |
| BseGI | GGATG | 4 cut(s) | 394, 472, 724, 961 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 581, 582, 652 |
| BseMI | GCAATG | 2 cut(s) | 222, 1065 |
| BseMII | CTCAG | 6 cut(s) | 132, 186, 702, 816, 1004, 1074 |
| BseNI | ACTGG | 7 cut(s) | 76, 316, 364, 632, 775, 817, 1040 |
| BseXI | GCAGC | 3 cut(s) | 437, 440, 961 |
| BsgI | GTGCAG | 1 cut(s) | 244 |
| BshFI | GGCC | 5 cut(s) | 42, 685, 807, 869, 1017 |
| BshNI | GGYRCC | 1 cut(s) | 743 |
| BshVI | ATCGAT | 1 cut(s) | 1110 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1089 |
| BsiHKCI | CYCGRG | 1 cut(s) | 650 |
| BsiSI | CCGG | 3 cut(s) | 375, 703, 1014 |
| BslFI | GGGAC | 3 cut(s) | 557, 624, 734 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 581, 582, 652 |
| BsmAI | GTCTC | 2 cut(s) | 236, 761 |
| BsmFI | GGGAC | 3 cut(s) | 557, 624, 734 |
| BsnI | GGCC | 5 cut(s) | 42, 685, 807, 869, 1017 |
| Bso31I | GGTCTC | 1 cut(s) | 236 |
| BsoBI | CYCGRG | 1 cut(s) | 650 |
| Bsp1286I | GDGCHC | 3 cut(s) | 678, 1089, 1139 |
| Bsp1407I | TGTACA | 1 cut(s) | 723 |
| Bsp143I | GATC | 7 cut(s) | 81, 666, 919, 958, 1098, 1107, 1111 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 195, 1083 |
| BspACI | CCGC | 2 cut(s) | 963, 987 |
| BspANI | GGCC | 5 cut(s) | 42, 685, 807, 869, 1017 |
| BspCNI | CTCAG | 6 cut(s) | 133, 187, 701, 817, 1003, 1075 |
| BspDI | ATCGAT | 1 cut(s) | 1110 |
| BspLI | GGNNCC | 5 cut(s) | 316, 639, 640, 745, 1136 |
| BspPI | GGATC | 4 cut(s) | 661, 927, 953, 1115 |
| BspT107I | GGYRCC | 1 cut(s) | 743 |
| BspTI | CTTAAG | 1 cut(s) | 188 |
| BspTNI | GGTCTC | 1 cut(s) | 236 |
| BsrBI | CCGCTC | 1 cut(s) | 989 |
| BsrDI | GCAATG | 2 cut(s) | 222, 1065 |
| BsrFI | RCCGGY | 1 cut(s) | 374 |
| BsrGI | TGTACA | 1 cut(s) | 723 |
| BsrI | ACTGG | 7 cut(s) | 76, 316, 364, 632, 775, 817, 1040 |
| BssAI | RCCGGY | 1 cut(s) | 374 |
| BssECI | CCNNGG | 4 cut(s) | 171, 384, 951, 1012 |
| BssMI | GATC | 7 cut(s) | 81, 666, 919, 958, 1098, 1107, 1111 |
| BssSI | CACGAG | 1 cut(s) | 671 |
| BssT1I | CCWWGG | 2 cut(s) | 384, 951 |
| Bst2BI | CACGAG | 1 cut(s) | 671 |
| Bst2UI | CCWGG | 4 cut(s) | 172, 279, 786, 876 |
| Bst4CI | ACNGT | 4 cut(s) | 265, 332, 748, 1052 |
| BstAFI | CTTAAG | 1 cut(s) | 188 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 412 |
| BstAUI | TGTACA | 1 cut(s) | 723 |
| BstC8I | GCNNGC | 2 cut(s) | 408, 417 |
| BstDEI | CTNAG | 6 cut(s) | 141, 195, 688, 825, 990, 1083 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 650 |
| BstF5I | GGATG | 4 cut(s) | 394, 472, 724, 961 |
| BstKTI | GATC | 7 cut(s) | 84, 669, 922, 961, 1101, 1110, 1114 |
| BstMAI | GTCTC | 2 cut(s) | 236, 761 |
| BstMBI | GATC | 7 cut(s) | 81, 666, 919, 958, 1098, 1107, 1111 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 332, 412, 416, 682, 703, 971 |
| BstNI | CCWGG | 4 cut(s) | 172, 279, 786, 876 |
| BstNSI | RCATGY | 1 cut(s) | 410 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 773 |
| BstSCI | CCNGG | 5 cut(s) | 170, 277, 784, 874, 1012 |
| BstV1I | GCAGC | 3 cut(s) | 437, 440, 961 |
| BstX2I | RGATCY | 2 cut(s) | 666, 919 |
| BstYI | RGATCY | 2 cut(s) | 666, 919 |
| Bsu15I | ATCGAT | 1 cut(s) | 1110 |
| BsuRI | GGCC | 5 cut(s) | 42, 685, 807, 869, 1017 |
| BsuTUI | ATCGAT | 1 cut(s) | 1110 |
| BtrI | CACGTC | 1 cut(s) | 761 |
| BtsCI | GGATG | 4 cut(s) | 394, 472, 724, 961 |
| BtsIMutI | CAGTG | 3 cut(s) | 69, 261, 357 |
| Cac8I | GCNNGC | 2 cut(s) | 408, 417 |
| Cfr10I | RCCGGY | 1 cut(s) | 374 |
| Cfr13I | GGNCC | 5 cut(s) | 612, 638, 684, 806, 868 |
| ClaI | ATCGAT | 1 cut(s) | 1110 |
| Csp6I | GTAC | 5 cut(s) | 253, 484, 631, 724, 744 |
| CspCI | CAANNNNNGTGG | 6 cut(s) | 76, 111, 604, 639, 715, 750 |
| CviAII | CATG | 5 cut(s) | 44, 304, 407, 601, 851 |
| CviQI | GTAC | 5 cut(s) | 253, 484, 631, 724, 744 |
| DdeI | CTNAG | 6 cut(s) | 141, 195, 688, 825, 990, 1083 |
| DpnI | GATC | 7 cut(s) | 83, 668, 921, 960, 1100, 1109, 1113 |
| DpnII | GATC | 7 cut(s) | 81, 666, 919, 958, 1098, 1107, 1111 |
| EaeI | YGGCCR | 2 cut(s) | 40, 1015 |
| Ecl136II | GAGCTC | 1 cut(s) | 1087 |
| Eco130I | CCWWGG | 2 cut(s) | 384, 951 |
| Eco24I | GRGCYC | 3 cut(s) | 678, 1089, 1139 |
| Eco31I | GGTCTC | 1 cut(s) | 236 |
| Eco47I | GGWCC | 2 cut(s) | 612, 638 |
| Eco53kI | GAGCTC | 1 cut(s) | 1087 |
| Eco57I | CTGAAG | 1 cut(s) | 698 |
| Eco88I | CYCGRG | 1 cut(s) | 650 |
| EcoICRI | GAGCTC | 1 cut(s) | 1087 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 650 |
| EcoO109I | RGGNCCY | 3 cut(s) | 638, 684, 868 |
| EcoRII | CCWGG | 4 cut(s) | 170, 277, 784, 874 |
| EcoT14I | CCWWGG | 2 cut(s) | 384, 951 |
| EcoT38I | GRGCYC | 3 cut(s) | 678, 1089, 1139 |
| ErhI | CCWWGG | 2 cut(s) | 384, 951 |
| FaeI | CATG | 5 cut(s) | 47, 307, 410, 604, 854 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 551, 583 |
| FaqI | GGGAC | 3 cut(s) | 557, 624, 734 |
| FatI | CATG | 5 cut(s) | 43, 303, 406, 600, 850 |
| FauI | CCCGC | 1 cut(s) | 970 |
| FblI | GTMKAC | 2 cut(s) | 48, 62 |
| Fnu4HI | GCNGC | 3 cut(s) | 451, 454, 975 |
| FokI | GGATG | 4 cut(s) | 401, 479, 731, 968 |
| FriOI | GRGCYC | 3 cut(s) | 678, 1089, 1139 |
| Fsp4HI | GCNGC | 3 cut(s) | 451, 454, 975 |
| GluI | GCNGC | 3 cut(s) | 451, 454, 975 |
| GsuI | CTGGAG | 2 cut(s) | 381, 1023 |
| HaeIII | GGCC | 5 cut(s) | 42, 685, 807, 869, 1017 |
| HapII | CCGG | 3 cut(s) | 375, 703, 1014 |
| Hin1II | CATG | 5 cut(s) | 47, 307, 410, 604, 854 |
| HincII | GTYRAC | 2 cut(s) | 49, 63 |
| HindII | GTYRAC | 2 cut(s) | 49, 63 |
| HindIII | AAGCTT | 2 cut(s) | 89, 185 |
| HinfI | GANTC | 4 cut(s) | 50, 350, 562, 891 |
| HpaII | CCGG | 3 cut(s) | 375, 703, 1014 |
| HphI | GGTGA | 3 cut(s) | 605, 808, 890 |
| Hpy166II | GTNNAC | 5 cut(s) | 49, 63, 203, 615, 724 |
| Hpy188I | TCNGA | 4 cut(s) | 142, 396, 691, 819 |
| Hpy188III | TCNNGA | 9 cut(s) | 127, 241, 287, 298, 535, 650, 659, 716, 1046 |
| Hpy8I | GTNNAC | 5 cut(s) | 49, 63, 203, 615, 724 |
| HpyAV | CCTTC | 6 cut(s) | 124, 508, 532, 656, 881, 1125 |
| HpyCH4III | ACNGT | 4 cut(s) | 265, 332, 748, 1052 |
| HpyCH4IV | ACGT | 3 cut(s) | 205, 760, 897 |
| HpyCH4V | TGCA | 4 cut(s) | 261, 456, 847, 862 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 332, 412, 416, 682, 703, 971 |
| HpyF3I | CTNAG | 6 cut(s) | 141, 195, 688, 825, 990, 1083 |
| HpySE526I | ACGT | 3 cut(s) | 205, 760, 897 |
| Hsp92II | CATG | 5 cut(s) | 47, 307, 410, 604, 854 |
| KflI | GGGWCCC | 1 cut(s) | 638 |
| KpnI | GGTACC | 1 cut(s) | 747 |
| Kzo9I | GATC | 7 cut(s) | 81, 666, 919, 958, 1098, 1107, 1111 |
| LmnI | GCTCC | 4 cut(s) | 280, 1042, 1122, 1134 |
| Lsp1109I | GCAGC | 3 cut(s) | 437, 440, 961 |
| LweI | GCATC | 3 cut(s) | 262, 457, 834 |
| MaeII | ACGT | 3 cut(s) | 205, 760, 897 |
| MaeIII | GTNAC | 6 cut(s) | 74, 287, 593, 796, 1052, 1090 |
| MalI | GATC | 7 cut(s) | 83, 668, 921, 960, 1100, 1109, 1113 |
| MbiI | CCGCTC | 1 cut(s) | 989 |
| MboI | GATC | 7 cut(s) | 81, 666, 919, 958, 1098, 1107, 1111 |
| MflI | RGATCY | 2 cut(s) | 666, 919 |
| MhlI | GDGCHC | 3 cut(s) | 678, 1089, 1139 |
| MlsI | TGGCCA | 1 cut(s) | 42 |
| MluCI | AATT | 1 cut(s) | 507 |
| MluNI | TGGCCA | 1 cut(s) | 42 |
| MlyI | GAGTC | 2 cut(s) | 44, 344 |
| MmeI | TCCRAC | 4 cut(s) | 159, 736, 868, 969 |
| Mox20I | TGGCCA | 1 cut(s) | 42 |
| MscI | TGGCCA | 1 cut(s) | 42 |
| MseI | TTAA | 3 cut(s) | 189, 780, 900 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1001 |
| Msp20I | TGGCCA | 1 cut(s) | 42 |
| MspCI | CTTAAG | 1 cut(s) | 188 |
| MspI | CCGG | 3 cut(s) | 375, 703, 1014 |
| MspR9I | CCNGG | 5 cut(s) | 172, 279, 786, 876, 1014 |
| MvaI | CCWGG | 4 cut(s) | 172, 279, 786, 876 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 332, 412, 416, 682, 703, 971 |
| NciI | CCSGG | 1 cut(s) | 1014 |
| NdeII | GATC | 7 cut(s) | 81, 666, 919, 958, 1098, 1107, 1111 |
| NlaIII | CATG | 5 cut(s) | 47, 307, 410, 604, 854 |
| NlaIV | GGNNCC | 5 cut(s) | 316, 639, 640, 745, 1136 |
| NmuCI | GTSAC | 5 cut(s) | 74, 287, 593, 796, 1052 |
| NspI | RCATGY | 1 cut(s) | 410 |
| PaeI | GCATGC | 1 cut(s) | 410 |
| PaeR7I | CTCGAG | 1 cut(s) | 650 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1107 |
| PfeI | GAWTC | 2 cut(s) | 562, 891 |
| PfoI | TCCNGGA | 1 cut(s) | 277 |
| PkrI | GCNGC | 3 cut(s) | 452, 455, 976 |
| PleI | GAGTC | 2 cut(s) | 44, 344 |
| PpsI | GAGTC | 2 cut(s) | 44, 344 |
| PpuMI | RGGWCCY | 1 cut(s) | 638 |
| PshAI | GACNNNNGTC | 1 cut(s) | 773 |
| Psp124BI | GAGCTC | 1 cut(s) | 1089 |
| Psp1406I | AACGTT | 1 cut(s) | 897 |
| Psp5II | RGGWCCY | 1 cut(s) | 638 |
| Psp6I | CCWGG | 4 cut(s) | 170, 277, 784, 874 |
| PspGI | CCWGG | 4 cut(s) | 170, 277, 784, 874 |
| PspN4I | GGNNCC | 5 cut(s) | 316, 639, 640, 745, 1136 |
| PspPI | GGNCC | 5 cut(s) | 612, 638, 684, 806, 868 |
| PspPPI | RGGWCCY | 1 cut(s) | 638 |
| PsuI | RGATCY | 2 cut(s) | 666, 919 |
| RsaI | GTAC | 5 cut(s) | 254, 485, 632, 725, 745 |
| RsaNI | GTAC | 5 cut(s) | 253, 484, 631, 724, 744 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1001 |
| SacI | GAGCTC | 1 cut(s) | 1089 |
| SalI | GTCGAC | 2 cut(s) | 47, 61 |
| SaqAI | TTAA | 3 cut(s) | 189, 780, 900 |
| SatI | GCNGC | 3 cut(s) | 451, 454, 975 |
| Sau3AI | GATC | 7 cut(s) | 81, 666, 919, 958, 1098, 1107, 1111 |
| Sau96I | GGNCC | 5 cut(s) | 612, 638, 684, 806, 868 |
| ScaI | AGTACT | 2 cut(s) | 254, 485 |
| SchI | GAGTC | 2 cut(s) | 44, 344 |
| ScrFI | CCNGG | 5 cut(s) | 172, 279, 786, 876, 1014 |
| SduI | GDGCHC | 3 cut(s) | 678, 1089, 1139 |
| SfaNI | GCATC | 3 cut(s) | 262, 457, 834 |
| Sfr274I | CTCGAG | 1 cut(s) | 650 |
| SinI | GGWCC | 2 cut(s) | 612, 638 |
| SlaI | CTCGAG | 1 cut(s) | 650 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1001 |
| SmlI | CTYRAG | 6 cut(s) | 188, 487, 533, 650, 923, 1126 |
| SmoI | CTYRAG | 6 cut(s) | 188, 487, 533, 650, 923, 1126 |
| SphI | GCATGC | 1 cut(s) | 410 |
| Sse9I | AATT | 1 cut(s) | 507 |
| SsiI | CCGC | 2 cut(s) | 963, 987 |
| SstI | GAGCTC | 1 cut(s) | 1089 |
| StyD4I | CCNGG | 5 cut(s) | 170, 277, 784, 874, 1012 |
| StyI | CCWWGG | 2 cut(s) | 384, 951 |
| TaaI | ACNGT | 4 cut(s) | 265, 332, 748, 1052 |
| TaiI | ACGT | 3 cut(s) | 208, 763, 900 |
| TasI | AATT | 1 cut(s) | 507 |
| TatI | WGTACW | 3 cut(s) | 252, 483, 723 |
| TfiI | GAWTC | 2 cut(s) | 562, 891 |
| Tru1I | TTAA | 3 cut(s) | 189, 780, 900 |
| Tru9I | TTAA | 3 cut(s) | 189, 780, 900 |
| TscAI | CASTG | 3 cut(s) | 76, 268, 364 |
| TseFI | GTSAC | 5 cut(s) | 74, 287, 593, 796, 1052 |
| TseI | GCWGC | 3 cut(s) | 450, 453, 974 |
| Tsp45I | GTSAC | 5 cut(s) | 74, 287, 593, 796, 1052 |
| TspDTI | ATGAA | 4 cut(s) | 161, 519, 867, 1019 |
| TspRI | CASTG | 3 cut(s) | 76, 268, 364 |
| Vha464I | CTTAAG | 1 cut(s) | 188 |
| VpaK11BI | GGWCC | 2 cut(s) | 612, 638 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 650 |
| XceI | RCATGY | 1 cut(s) | 410 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 50 |
| XhoI | CTCGAG | 1 cut(s) | 650 |
| XmiI | GTMKAC | 2 cut(s) | 48, 62 |
| ZrmI | AGTACT | 2 cut(s) | 254, 485 |