RLG00000035365

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000682
Physical Location & Seq
Forward (+)
65962553 .. 65963845
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035365

Sequence Viewer

Length: 1200 bp
ATGAACAAAAGGTCATTCAAGTATGCCTGGGTGTTGGACAAGCTCAAGGCTGAGCGTGAGCGTGGTATCACCATTGATATTGCCTTGTGGAAGTTTGAGACCACCAAGTACTACTGCACTGTCATCGATGCTCCCGGACATCGTGATTTCATCAAGAACATGATTACTGGTACCTCCCAGGCTGATTGTGCCGTCCTCATCATCGACTCCACCACTGGTGGTTTTGAAGCTGGTATCTCCAAGGATGGTCAGACCCGTGAGCACGCTCTGCTTGCTTTCACTCTTGGTGTCAAGCAGATGATTTGCTGCTGTAACAAGATGGATGCCACCACTCCCAAGTACTCAAAGGCAAGGTATGATGAAATTGTGAAGGAAGTGTCTTCCTACCTGAAGAAGGTTGGGTACAACCCAGACAAAATTGCCTTTGTTCCCATCTCTGGATTCGAGGGTGACAACATGATTGAGAGGTCCACCAACCTCGACTGGTACAAGGGCCCTACCCTCCTCGAGGCACTTGACCAAATCAATGAGCCCAAGAGGCCCTCAGACAAGCCCCTCCGTCTCCCACTTCAGGATGTGTACAAGATTGGTGGCATTGGAACTGTGCCTGTGGGACGTGTTGAGACCGGTATCATCAAGCCTGGTATGGTTGTGACTTTTGGCCCCACTGGACTGACTACTGAAGTGAAGTCTGTGGAGATGCACCACGAAGCTCTACAGGAGGCTCTTCCTGGTGACAATGTTGGGTTCAATGTGAAGAATGTTGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCATCCAACTCCAAGGATGACCCTGCCAAGGAAGCTGCTAACTTCACCTCACAGGTCATCATCATGAACCACCCTGGCCAGATTGGAAATGGATATGCTCCGGTGCTCGACTGCCACACCTCCCACATTGCTGTTAAGTTTGGTGAGATTTTGACCAAGATTGACAGGCGATCTGGTAAGGAGATTGAGAAGGAGCCCAAGTTCTTGAAGAATGGTGATGCTGGTATGGTTAAGATGCTTCCCACCAAGCCCATGGTTGTTGAGACCTTCTCTGAGTACCCTCCTCTTGGTCGTTTTGCTGTGAGGGACATGCGTCAGACAGTTGCTGTTGGAGTCATCAAGAATGTTGAGAAGAAGGATCCATCTGGTGCCAAGGTGACCAAGTCTGCTGCTAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

400

Amino Acids

44.02

Weight (kDa)

9.14

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 8 - 175 4.4e-43 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 200 - 265 2.2e-17 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 274 - 381 7.4e-27 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 279 - 379 6.1e-37 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 506
Acc65I GGTACC 1 cut(s) 170
AccB1I GGYRCC 2 cut(s) 170, 1163
AclWI GGATC 3 cut(s) 783, 1148, 1161
AcoI YGGCCR 1 cut(s) 871
AcuI CTGAAG 3 cut(s) 410, 554, 702
AfaI GTAC 7 cut(s) 110, 172, 341, 404, 488, 581, 1073
AfiI CCNNNNNNNGG 6 cut(s) 394, 437, 508, 571, 823, 1082
AflIII ACRYGT 1 cut(s) 616
AgeI ACCGGT 1 cut(s) 626
AgsI TTSAA 4 cut(s) 19, 227, 751, 1003
AjiI CACGTC 1 cut(s) 617
AjnI CCWGG 5 cut(s) 26, 177, 640, 730, 868
AloI GAACNNNNNNTCC 2 cut(s) 980, 1012
AluBI AGCT 4 cut(s) 43, 230, 713, 830
AluI AGCT 4 cut(s) 43, 230, 713, 830
Alw21I GWGCWC 2 cut(s) 264, 903
Alw26I GTCTC 4 cut(s) 92, 566, 617, 1052
AlwI GGATC 3 cut(s) 783, 1148, 1161
AlwNI CAGNNNCTG 1 cut(s) 1121
Ama87I CYCGRG 1 cut(s) 506
AoxI GGCC 4 cut(s) 493, 539, 661, 871
ApaI GGGCCC 1 cut(s) 497
ApeKI GCWGC 3 cut(s) 306, 830, 1184
AsiGI ACCGGT 1 cut(s) 626
Asp718I GGTACC 1 cut(s) 170
AspS9I GGNCC 5 cut(s) 468, 493, 494, 540, 662
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 7 cut(s) 61, 461, 746, 832, 950, 1022, 1183
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 1 cut(s) 468
BaeGI GKGCMC 1 cut(s) 497
BalI TGGCCA 1 cut(s) 873
BamHI GGATCC 1 cut(s) 1153
BanI GGYRCC 2 cut(s) 170, 1163
BanII GRGCYC 3 cut(s) 497, 534, 993
BarI GAAGNNNNNNTAC 2 cut(s) 386, 418
BbsI GAAGAC 1 cut(s) 372
Bbv12I GWGCWC 2 cut(s) 264, 903
BbvI GCAGC 3 cut(s) 293, 817, 1171
BccI CCATC 4 cut(s) 239, 313, 440, 1165
BceAI ACGGC 1 cut(s) 176
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 5 cut(s) 28, 179, 642, 732, 870
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 4 cut(s) 92, 566, 617, 1052
BfmI CTRYAG 1 cut(s) 716
BglI GCCNNNNNGGC 1 cut(s) 538
BisI GCNGC 3 cut(s) 307, 831, 1185
BlpI GCTNAGC 1 cut(s) 51
BlsI GCNGC 3 cut(s) 308, 832, 1186
BmcAI AGTACT 2 cut(s) 110, 341
Bme1390I CCNGG 6 cut(s) 28, 135, 179, 642, 732, 870
Bme18I GGWCC 1 cut(s) 468
BmeT110I CYCGRG 1 cut(s) 506
BmgBI CACGTC 1 cut(s) 617
BmgT120I GGNCC 5 cut(s) 468, 493, 494, 540, 662
BmiI GGNNCC 6 cut(s) 172, 495, 664, 990, 1155, 1165
BmrFI CCNGG 6 cut(s) 28, 135, 179, 642, 732, 870
BmsI GCATC 6 cut(s) 118, 313, 690, 806, 1003, 1020
BoxI GACNNNNGTC 1 cut(s) 1107
BpiI GAAGAC 1 cut(s) 372
BplI GAGNNNNNCTC 2 cut(s) 1049, 1081
Bpu1102I GCTNAGC 1 cut(s) 51
BpuEI CTTGAG 2 cut(s) 29, 764
BpuMI CCSGG 1 cut(s) 135
Bsa29I ATCGAT 1 cut(s) 126
BsaI GGTCTC 3 cut(s) 92, 617, 1052
BsaJI CCNNGG 8 cut(s) 27, 177, 240, 807, 822, 868, 1047, 1167
BsaWI WCCGGW 2 cut(s) 626, 895
BsaXI ACNNNNNCTCC 2 cut(s) 980, 1010
Bsc4I CCNNNNNNNGG 6 cut(s) 394, 437, 508, 571, 823, 1082
Bse118I RCCGGY 1 cut(s) 626
Bse1I ACTGG 4 cut(s) 172, 220, 488, 673
Bse3DI GCAATG 1 cut(s) 921
BseBI CCWGG 5 cut(s) 28, 179, 642, 732, 870
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 8 cut(s) 27, 177, 240, 807, 822, 868, 1047, 1167
BseGI GGATG 5 cut(s) 250, 328, 580, 797, 817
BseLI CCNNNNNNNGG 6 cut(s) 394, 437, 508, 571, 823, 1082
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 3 cut(s) 42, 558, 1059
BseNI ACTGG 4 cut(s) 172, 220, 488, 673
BseRI GAGGAG 2 cut(s) 494, 1068
BseSI GKGCMC 1 cut(s) 497
BseXI GCAGC 3 cut(s) 293, 817, 1171
BsgI GTGCAG 1 cut(s) 100
BshFI GGCC 4 cut(s) 495, 541, 663, 873
BshNI GGYRCC 2 cut(s) 170, 1163
BshTI ACCGGT 1 cut(s) 626
BshVI ATCGAT 1 cut(s) 126
BsiHKAI GWGCWC 2 cut(s) 264, 903
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 3 cut(s) 135, 627, 896
BslFI GGGAC 2 cut(s) 627, 1115
BslI CCNNNNNNNGG 6 cut(s) 394, 437, 508, 571, 823, 1082
BsmAI GTCTC 4 cut(s) 92, 566, 617, 1052
BsmBI CGTCTC 1 cut(s) 566
BsmFI GGGAC 2 cut(s) 627, 1115
BsnI GGCC 4 cut(s) 495, 541, 663, 873
Bso31I GGTCTC 3 cut(s) 92, 617, 1052
BsoBI CYCGRG 1 cut(s) 506
Bsp120I GGGCCC 1 cut(s) 493
Bsp1286I GDGCHC 5 cut(s) 264, 497, 534, 903, 993
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 3 cut(s) 775, 965, 1153
Bsp1720I GCTNAGC 1 cut(s) 51
Bsp19I CCATGG 1 cut(s) 1047
BspANI GGCC 4 cut(s) 495, 541, 663, 873
BspCNI CTCAG 3 cut(s) 43, 557, 1060
BspDI ATCGAT 1 cut(s) 126
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 6 cut(s) 172, 495, 664, 990, 1155, 1165
BspPI GGATC 3 cut(s) 783, 1148, 1161
BspQI GCTCTTC 1 cut(s) 732
BspT107I GGYRCC 2 cut(s) 170, 1163
BspTNI GGTCTC 3 cut(s) 92, 617, 1052
BsrDI GCAATG 1 cut(s) 921
BsrFI RCCGGY 1 cut(s) 626
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 4 cut(s) 172, 220, 488, 673
BssAI RCCGGY 1 cut(s) 626
BssECI CCNNGG 8 cut(s) 27, 177, 240, 807, 822, 868, 1047, 1167
BssMI GATC 3 cut(s) 775, 965, 1153
BssT1I CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
Bst2UI CCWGG 5 cut(s) 28, 179, 642, 732, 870
Bst4CI ACNGT 3 cut(s) 121, 604, 1117
Bst6I CTCTTC 1 cut(s) 732
BstAPI GCANNNNNTGC 1 cut(s) 268
BstAUI TGTACA 1 cut(s) 579
BstC8I GCNNGC 2 cut(s) 264, 273
BstDEI CTNAG 4 cut(s) 51, 544, 1068, 1188
BstDSI CCRYGG 1 cut(s) 1047
BstEII GGTNACC 1 cut(s) 1171
BstENI CCTNNNNNAGG 2 cut(s) 392, 506
BstF5I GGATG 5 cut(s) 250, 328, 580, 797, 817
BstKTI GATC 3 cut(s) 778, 968, 1156
BstMAI GTCTC 4 cut(s) 92, 566, 617, 1052
BstMBI GATC 3 cut(s) 775, 965, 1153
BstMWI GCNNNNNNNGC 5 cut(s) 188, 268, 272, 538, 827
BstNI CCWGG 5 cut(s) 28, 179, 642, 732, 870
BstNSI RCATGY 1 cut(s) 1108
BstPAI GACNNNNGTC 1 cut(s) 1107
BstPI GGTNACC 1 cut(s) 1171
BstSCI CCNGG 6 cut(s) 26, 133, 177, 640, 730, 868
BstSFI CTRYAG 1 cut(s) 716
BstSLI GKGCMC 1 cut(s) 497
BstV1I GCAGC 3 cut(s) 293, 817, 1171
BstV2I GAAGAC 1 cut(s) 372
BstX2I RGATCY 2 cut(s) 775, 1153
BstXI CCANNNNNNTGG 1 cut(s) 1048
BstYI RGATCY 2 cut(s) 775, 1153
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 4 cut(s) 495, 541, 663, 873
BsuTUI ATCGAT 1 cut(s) 126
BtgI CCRYGG 1 cut(s) 1047
BtrI CACGTC 1 cut(s) 617
BtsCI GGATG 5 cut(s) 250, 328, 580, 797, 817
BtsIMutI CAGTG 3 cut(s) 117, 213, 666
Cac8I GCNNGC 2 cut(s) 264, 273
CaiI CAGNNNCTG 1 cut(s) 1121
CciI TCATGA 1 cut(s) 858
Cfr10I RCCGGY 1 cut(s) 626
Cfr13I GGNCC 5 cut(s) 468, 493, 494, 540, 662
ClaI ATCGAT 1 cut(s) 126
CseI GACGC 1 cut(s) 1097
Csp6I GTAC 7 cut(s) 109, 171, 340, 403, 487, 580, 1072
CspAI ACCGGT 1 cut(s) 626
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 5 cut(s) 160, 457, 859, 1048, 1105
CviQI GTAC 7 cut(s) 109, 171, 340, 403, 487, 580, 1072
DdeI CTNAG 4 cut(s) 51, 544, 1068, 1188
DpnI GATC 3 cut(s) 777, 967, 1155
DpnII GATC 3 cut(s) 775, 965, 1153
EaeI YGGCCR 1 cut(s) 871
Eam1104I CTCTTC 1 cut(s) 732
EarI CTCTTC 1 cut(s) 732
Eco130I CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
Eco24I GRGCYC 3 cut(s) 497, 534, 993
Eco31I GGTCTC 3 cut(s) 92, 617, 1052
Eco47I GGWCC 1 cut(s) 468
Eco57I CTGAAG 3 cut(s) 410, 554, 702
Eco88I CYCGRG 1 cut(s) 506
Eco91I GGTNACC 1 cut(s) 1171
EcoNI CCTNNNNNAGG 2 cut(s) 392, 506
EcoO109I RGGNCCY 3 cut(s) 493, 494, 540
EcoO65I GGTNACC 1 cut(s) 1171
EcoRII CCWGG 5 cut(s) 26, 177, 640, 730, 868
EcoT14I CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
EcoT38I GRGCYC 3 cut(s) 497, 534, 993
ErhI CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 5 cut(s) 163, 460, 862, 1051, 1108
FaqI GGGAC 2 cut(s) 627, 1115
FatI CATG 5 cut(s) 159, 456, 858, 1047, 1104
Fnu4HI GCNGC 3 cut(s) 307, 831, 1185
FokI GGATG 5 cut(s) 257, 335, 587, 784, 824
FriOI GRGCYC 3 cut(s) 497, 534, 993
Fsp4HI GCNGC 3 cut(s) 307, 831, 1185
GluI GCNGC 3 cut(s) 307, 831, 1185
HaeIII GGCC 4 cut(s) 495, 541, 663, 873
HapII CCGG 3 cut(s) 135, 627, 896
HgaI GACGC 1 cut(s) 1097
Hin1II CATG 5 cut(s) 163, 460, 862, 1051, 1108
HinfI GANTC 3 cut(s) 206, 441, 1128
HpaII CCGG 3 cut(s) 135, 627, 896
HphI GGTGA 7 cut(s) 61, 461, 746, 832, 950, 1022, 1183
Hpy166II GTNNAC 2 cut(s) 471, 580
Hpy188I TCNGA 4 cut(s) 252, 547, 1069, 1113
Hpy188III TCNNGA 7 cut(s) 143, 154, 438, 572, 859, 1000, 1135
Hpy8I GTNNAC 2 cut(s) 471, 580
HpyAV CCTTC 5 cut(s) 364, 388, 979, 1072, 1144
HpyCH4III ACNGT 3 cut(s) 121, 604, 1117
HpyCH4IV ACGT 1 cut(s) 616
HpyCH4V TGCA 3 cut(s) 117, 703, 797
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 268, 272, 538, 827
HpyF3I CTNAG 4 cut(s) 51, 544, 1068, 1188
HpySE526I ACGT 1 cut(s) 616
Hsp92II CATG 5 cut(s) 163, 460, 862, 1051, 1108
KpnI GGTACC 1 cut(s) 174
Kzo9I GATC 3 cut(s) 775, 965, 1153
LguI GCTCTTC 1 cut(s) 732
LmnI GCTCC 3 cut(s) 136, 898, 988
Lsp1109I GCAGC 3 cut(s) 293, 817, 1171
LweI GCATC 6 cut(s) 118, 313, 690, 806, 1003, 1020
MaeII ACGT 1 cut(s) 616
MaeIII GTNAC 5 cut(s) 311, 449, 652, 734, 1171
MalI GATC 3 cut(s) 777, 967, 1155
MboI GATC 3 cut(s) 775, 965, 1153
MboII GAAGA 6 cut(s) 372, 403, 719, 769, 1015, 1159
MflI RGATCY 2 cut(s) 775, 1153
MhlI GDGCHC 5 cut(s) 264, 497, 534, 903, 993
MlsI TGGCCA 1 cut(s) 873
MluCI AATT 2 cut(s) 363, 417
MluNI TGGCCA 1 cut(s) 873
MlyI GAGTC 2 cut(s) 200, 1137
MmeI TCCRAC 3 cut(s) 15, 825, 1105
Mox20I TGGCCA 1 cut(s) 873
MscI TGGCCA 1 cut(s) 873
MseI TTAA 2 cut(s) 930, 1026
MslI CAYNNNNRTG 1 cut(s) 857
Msp20I TGGCCA 1 cut(s) 873
MspI CCGG 3 cut(s) 135, 627, 896
MspR9I CCNGG 6 cut(s) 28, 135, 179, 642, 732, 870
MvaI CCWGG 5 cut(s) 28, 179, 642, 732, 870
MwoI GCNNNNNNNGC 5 cut(s) 188, 268, 272, 538, 827
NciI CCSGG 1 cut(s) 135
NcoI CCATGG 1 cut(s) 1047
NdeII GATC 3 cut(s) 775, 965, 1153
NlaIII CATG 5 cut(s) 163, 460, 862, 1051, 1108
NlaIV GGNNCC 6 cut(s) 172, 495, 664, 990, 1155, 1165
NmuCI GTSAC 4 cut(s) 449, 652, 734, 1171
NspI RCATGY 1 cut(s) 1108
PaeR7I CTCGAG 1 cut(s) 506
PagI TCATGA 1 cut(s) 858
PciSI GCTCTTC 1 cut(s) 732
PfeI GAWTC 1 cut(s) 441
PflFI GACNNNGTC 1 cut(s) 1177
PfoI TCCNGGA 1 cut(s) 133
PinAI ACCGGT 1 cut(s) 626
PkrI GCNGC 3 cut(s) 308, 832, 1186
PleI GAGTC 2 cut(s) 200, 1136
PpsI GAGTC 2 cut(s) 200, 1136
PshAI GACNNNNGTC 1 cut(s) 1107
Psp6I CCWGG 5 cut(s) 26, 177, 640, 730, 868
PspEI GGTNACC 1 cut(s) 1171
PspGI CCWGG 5 cut(s) 26, 177, 640, 730, 868
PspN4I GGNNCC 6 cut(s) 172, 495, 664, 990, 1155, 1165
PspOMI GGGCCC 1 cut(s) 493
PspPI GGNCC 5 cut(s) 468, 493, 494, 540, 662
PspXI VCTCGAGB 1 cut(s) 506
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 2 cut(s) 775, 1153
PsyI GACNNNGTC 1 cut(s) 1177
RsaI GTAC 7 cut(s) 110, 172, 341, 404, 488, 581, 1073
RsaNI GTAC 7 cut(s) 109, 171, 340, 403, 487, 580, 1072
RseI CAYNNNNRTG 1 cut(s) 857
SapI GCTCTTC 1 cut(s) 732
SaqAI TTAA 2 cut(s) 930, 1026
SatI GCNGC 3 cut(s) 307, 831, 1185
Sau3AI GATC 3 cut(s) 775, 965, 1153
Sau96I GGNCC 5 cut(s) 468, 493, 494, 540, 662
ScaI AGTACT 2 cut(s) 110, 341
SchI GAGTC 2 cut(s) 200, 1137
ScrFI CCNGG 6 cut(s) 28, 135, 179, 642, 732, 870
SduI GDGCHC 5 cut(s) 264, 497, 534, 903, 993
SfaNI GCATC 6 cut(s) 118, 313, 690, 806, 1003, 1020
SfcI CTRYAG 1 cut(s) 716
Sfr274I CTCGAG 1 cut(s) 506
SinI GGWCC 1 cut(s) 468
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 1 cut(s) 857
SmlI CTYRAG 3 cut(s) 44, 506, 779
SmoI CTYRAG 3 cut(s) 44, 506, 779
Sse9I AATT 2 cut(s) 363, 417
StyD4I CCNGG 6 cut(s) 26, 133, 177, 640, 730, 868
StyI CCWWGG 5 cut(s) 240, 807, 822, 1047, 1167
TaaI ACNGT 3 cut(s) 121, 604, 1117
TaiI ACGT 1 cut(s) 619
TaqI TCGA 6 cut(s) 126, 204, 444, 480, 507, 903
TasI AATT 2 cut(s) 363, 417
TatI WGTACW 3 cut(s) 108, 339, 579
TfiI GAWTC 1 cut(s) 441
Tru1I TTAA 2 cut(s) 930, 1026
Tru9I TTAA 2 cut(s) 930, 1026
TscAI CASTG 3 cut(s) 124, 220, 673
TseFI GTSAC 4 cut(s) 449, 652, 734, 1171
TseI GCWGC 3 cut(s) 306, 830, 1184
Tsp45I GTSAC 4 cut(s) 449, 652, 734, 1171
TspDTI ATGAA 4 cut(s) 17, 139, 375, 875
TspGWI ACGGA 1 cut(s) 548
TspRI CASTG 3 cut(s) 124, 220, 673
Tth111I GACNNNGTC 1 cut(s) 1177
VpaK11BI GGWCC 1 cut(s) 468
XagI CCTNNNNNAGG 2 cut(s) 392, 506
XceI RCATGY 1 cut(s) 1108
XcmI CCANNNNNNNNNTGG 2 cut(s) 881, 1045
XhoI CTCGAG 1 cut(s) 506
ZrmI AGTACT 2 cut(s) 110, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.