MD04G1011900.v1.1

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0000682
Physical Location & Seq
Reverse (-)
1342274 .. 1345372
3099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1011900.v1.1.491

Sequence Viewer

Length: 603 bp
ATGGGTAAAGAGAAGTTTCACATCAACATCGTGGTCATTGGCCATGTCGACTCCGGCAAGTCGACCACCACTGGTCATCTTATCTACAAGCTTGGCGGTATTGACAAGCGTGTGATTGAGAGGTTCGAGAAGGAAGCTGCTGAGATGAACAAAAGGTCGTTCAAGTATGCCTGGGTGCTTGACAAGCTTAAGGCTGAGCGTGAGCGTGGTATCACCATTGATATCGCCTTGTGGAAGTTTGAGACCACAAAGTACTACTGCACGGTCATTGATGCTCCAGGGCATCGTGATTTCATTAAGAACATGATTACTGGAACTTCACAGGCTGACTGTGCTGTCCTCATCATTGACTCCACCACTGGTGGTTTCGAAGCTGGTATTTCCAAGGACGGACAGACCCGTGAGCACGCTCTTCTTGCTTTCACCCTCGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGATGCCACCACTCCTAAGTACTCCAAGGCAAGGTACGAGGAAATCGTGAAGGAAGTTTCATCCTACCTGAAGAAGGAGGATGTTTTTGAGTTGAGACTATTTACTTATCAAGTTTTGTTGCGTCTGTTGAATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

201

Amino Acids

22.72

Weight (kDa)

8.85

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 182 8e-53 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AccI GTMKAC 2 cut(s) 48, 62
AciI CCGC 1 cut(s) 96
AcoI YGGCCR 1 cut(s) 40
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 3 cut(s) 254, 485, 500
AfiI CCNNNNNNNGG 2 cut(s) 495, 538
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 2 cut(s) 163, 595
AjnI CCWGG 2 cut(s) 170, 277
AluBI AGCT 4 cut(s) 91, 137, 187, 374
AluI AGCT 4 cut(s) 91, 137, 187, 374
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 2 cut(s) 236, 553
AoxI GGCC 1 cut(s) 40
ApeKI GCWGC 2 cut(s) 137, 450
AsuHPI GGTGA 2 cut(s) 205, 415
AsuII TTCGAA 1 cut(s) 369
BalI TGGCCA 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 2 cut(s) 124, 437
BccI CCATC 1 cut(s) 457
BciT130I CCWGG 2 cut(s) 172, 279
BcoDI GTCTC 2 cut(s) 236, 553
BfrI CTTAAG 1 cut(s) 188
BisI GCNGC 2 cut(s) 138, 451
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 2 cut(s) 139, 452
BmcAI AGTACT 2 cut(s) 254, 485
Bme1390I CCNGG 2 cut(s) 172, 279
BmrFI CCNGG 2 cut(s) 172, 279
BmsI GCATC 3 cut(s) 262, 292, 457
BpmI CTGGAG 1 cut(s) 261
Bpu1102I GCTNAGC 1 cut(s) 195
Bpu14I TTCGAA 1 cut(s) 369
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 5 cut(s) 171, 278, 384, 427, 489
Bsc4I CCNNNNNNNGG 2 cut(s) 495, 538
Bse1I ACTGG 3 cut(s) 76, 316, 364
BseBI CCWGG 2 cut(s) 172, 279
BseDI CCNNGG 5 cut(s) 171, 278, 384, 427, 489
BseGI GGATG 3 cut(s) 472, 524, 550
BseLI CCNNNNNNNGG 2 cut(s) 495, 538
BseMII CTCAG 2 cut(s) 132, 186
BseNI ACTGG 3 cut(s) 76, 316, 364
BseXI GCAGC 2 cut(s) 124, 437
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 1 cut(s) 54
BslI CCNNNNNNNGG 2 cut(s) 495, 538
BsmAI GTCTC 2 cut(s) 236, 553
BsnI GGCC 1 cut(s) 42
Bso31I GGTCTC 1 cut(s) 236
Bsp119I TTCGAA 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 408
Bsp1720I GCTNAGC 1 cut(s) 195
BspACI CCGC 1 cut(s) 96
BspANI GGCC 1 cut(s) 42
BspCNI CTCAG 2 cut(s) 133, 187
BspQI GCTCTTC 1 cut(s) 417
BspT104I TTCGAA 1 cut(s) 369
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 236
BsrI ACTGG 3 cut(s) 76, 316, 364
BssECI CCNNGG 5 cut(s) 171, 278, 384, 427, 489
BssT1I CCWWGG 2 cut(s) 384, 489
Bst2UI CCWGG 2 cut(s) 172, 279
Bst4CI ACNGT 2 cut(s) 265, 332
Bst6I CTCTTC 1 cut(s) 417
BstAFI CTTAAG 1 cut(s) 188
BstBI TTCGAA 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 3 cut(s) 141, 195, 480
BstENI CCTNNNNNAGG 1 cut(s) 536
BstF5I GGATG 3 cut(s) 472, 524, 550
BstMAI GTCTC 2 cut(s) 236, 553
BstMWI GCNNNNNNNGC 3 cut(s) 184, 332, 416
BstNI CCWGG 2 cut(s) 172, 279
BstSCI CCNGG 2 cut(s) 170, 277
BstV1I GCAGC 2 cut(s) 124, 437
BsuRI GGCC 1 cut(s) 42
BtsCI GGATG 3 cut(s) 472, 524, 550
BtsIMutI CAGTG 2 cut(s) 69, 357
Cac8I GCNNGC 1 cut(s) 408
CseI GACGC 1 cut(s) 575
Csp6I GTAC 3 cut(s) 253, 484, 499
CviAII CATG 2 cut(s) 44, 304
CviJI RGCY 7 cut(s) 42, 91, 137, 187, 194, 326, 374
CviKI_1 RGCY 7 cut(s) 42, 91, 137, 187, 194, 326, 374
CviQI GTAC 3 cut(s) 253, 484, 499
DdeI CTNAG 3 cut(s) 141, 195, 480
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 1 cut(s) 417
EarI CTCTTC 1 cut(s) 417
Eco130I CCWWGG 2 cut(s) 384, 489
Eco31I GGTCTC 1 cut(s) 236
Eco32I GATATC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 554
EcoNI CCTNNNNNAGG 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 170, 277
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 384, 489
ErhI CCWWGG 2 cut(s) 384, 489
FaeI CATG 2 cut(s) 47, 307
FaiI YATR 3 cut(s) 45, 168, 305
FatI CATG 2 cut(s) 43, 303
FblI GTMKAC 2 cut(s) 48, 62
Fnu4HI GCNGC 2 cut(s) 138, 451
FokI GGATG 3 cut(s) 479, 511, 557
Fsp4HI GCNGC 2 cut(s) 138, 451
GluI GCNGC 2 cut(s) 138, 451
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 1 cut(s) 42
HapII CCGG 1 cut(s) 54
HgaI GACGC 1 cut(s) 575
Hin1II CATG 2 cut(s) 47, 307
HincII GTYRAC 2 cut(s) 49, 63
HindII GTYRAC 2 cut(s) 49, 63
HindIII AAGCTT 2 cut(s) 89, 185
HinfI GANTC 2 cut(s) 50, 350
HpaII CCGG 1 cut(s) 54
HphI GGTGA 2 cut(s) 205, 415
Hpy166II GTNNAC 2 cut(s) 49, 63
Hpy188III TCNNGA 3 cut(s) 127, 287, 511
Hpy8I GTNNAC 2 cut(s) 49, 63
HpyAV CCTTC 3 cut(s) 124, 508, 532
HpyCH4III ACNGT 2 cut(s) 265, 332
HpyCH4V TGCA 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 3 cut(s) 184, 332, 416
HpyF3I CTNAG 3 cut(s) 141, 195, 480
Hsp92II CATG 2 cut(s) 47, 307
LguI GCTCTTC 1 cut(s) 417
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 2 cut(s) 124, 437
LweI GCATC 3 cut(s) 262, 292, 457
MaeIII GTNAC 1 cut(s) 455
MboII GAAGA 2 cut(s) 404, 547
MhlI GDGCHC 1 cut(s) 408
MlsI TGGCCA 1 cut(s) 42
MluNI TGGCCA 1 cut(s) 42
MlyI GAGTC 2 cut(s) 44, 344
MnlI CCTC 5 cut(s) 114, 350, 437, 496, 535
Mox20I TGGCCA 1 cut(s) 42
MscI TGGCCA 1 cut(s) 42
MseI TTAA 2 cut(s) 189, 297
Msp20I TGGCCA 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 1 cut(s) 54
MspR9I CCNGG 2 cut(s) 172, 279
MvaI CCWGG 2 cut(s) 172, 279
MwoI GCNNNNNNNGC 3 cut(s) 184, 332, 416
NlaIII CATG 2 cut(s) 47, 307
NspV TTCGAA 1 cut(s) 369
PciSI GCTCTTC 1 cut(s) 417
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PkrI GCNGC 2 cut(s) 139, 452
PleI GAGTC 2 cut(s) 44, 344
PpsI GAGTC 2 cut(s) 44, 344
Psp6I CCWGG 2 cut(s) 170, 277
PspGI CCWGG 2 cut(s) 170, 277
RsaI GTAC 3 cut(s) 254, 485, 500
RsaNI GTAC 3 cut(s) 253, 484, 499
SalI GTCGAC 2 cut(s) 47, 61
SapI GCTCTTC 1 cut(s) 417
SaqAI TTAA 2 cut(s) 189, 297
SatI GCNGC 2 cut(s) 138, 451
ScaI AGTACT 2 cut(s) 254, 485
SchI GAGTC 2 cut(s) 44, 344
ScrFI CCNGG 2 cut(s) 172, 279
SduI GDGCHC 1 cut(s) 408
SetI ASST 8 cut(s) 93, 125, 139, 158, 189, 376, 500, 534
SfaNI GCATC 3 cut(s) 262, 292, 457
SfuI TTCGAA 1 cut(s) 369
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
SsiI CCGC 1 cut(s) 96
StyD4I CCNGG 2 cut(s) 170, 277
StyI CCWWGG 2 cut(s) 384, 489
TaaI ACNGT 2 cut(s) 265, 332
TaqI TCGA 4 cut(s) 48, 62, 126, 369
TatI WGTACW 2 cut(s) 252, 483
Tru1I TTAA 2 cut(s) 189, 297
Tru9I TTAA 2 cut(s) 189, 297
TscAI CASTG 2 cut(s) 76, 364
TseI GCWGC 2 cut(s) 137, 450
TspDTI ATGAA 3 cut(s) 161, 283, 513
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 2 cut(s) 76, 364
Vha464I CTTAAG 1 cut(s) 188
XagI CCTNNNNNAGG 1 cut(s) 536
XmiI GTMKAC 2 cut(s) 48, 62
ZrmI AGTACT 2 cut(s) 254, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.