MD03G1103900.v1.1

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000682
Physical Location & Seq
Reverse (-)
8944885 .. 8947532
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1103900.v1.1.491

Sequence Viewer

Length: 1344 bp
ATGGGTAAAGAGAAGTTCCATATCAACATTGTGGTCATTGGCCATGTCGACTCTGGCAAGTCAACCACCACTGGCCACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGAGGTCATTCAAGTATGCCTGGGTGTTGGACAAGCTCAAGGCTGAGCGTGAGCGTGGAATTACCATTGATATTGCCTTGTGGAAATTTGAGACCACCAAGTACTACTGCACTGTCATTGATGCTCCTGGACATCGTGACTTTATCAAGAACATGATTACGGGAACCTCCCAGGCTGACTGTGCCATCCTCATCATTGACTCCACCACTGGAGGTTTTGAAGCTGGTATTTCCAAGGATGGTCAGACCCGTGAGCATGCTTTGCTTGCTTTCACTCTTGGTGTCAAGCAAATGATTTGCTGCTGCAACAAGATGGATGCCACCACTCCCAAGTACTCAAAGGCAAGGTACGAAGAAATTGTGAAGGAAGTGTCGTCCTATCTCAAGAAGGTTGGCTACAACCCAGATAAGATTCCCTTTGTCCCCATTTCTGGGTTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGTCCCACCCTTCTTGAGGCTCTTGACCAGATCAACGAGCCCAAGAGGCCCTCAGACAAGCCCCTCCGGCTTCCACTTCAGGATGTGTACAAGATTGGTGGTATTGGTACCGTCCCTGTTGGACGAGTTGAGACTGGTGTCATTAAGCCTGGTATGGTGGTGACTTTTGGCCCAACTGGGCTAACTACTGAGGTCAAGTCTGTTGAGATGCACCACGAAGCTATGCTGGAGGCCCTTCCAGGTGATAATGTTGGATTCAACGTGAAGAATGTTGCTGTCAAGGATCTCAAGCGTGGTTATGTTGCTTCCAACTCCAAGGATGATCCTGCAAAGGAGGCTGCTAACTTTACCTCTCAGGTCATCATCATGAACCACCCCGGGCAGATTGGACAGGGATATGCACCAGTTCTCGACTGTCATACCTCCCACATTGCTGTCAAGTTTGCTGAGCTTGTGACAAAGATTGACAGGCGATCTGGCAAGGAGCTCGAGAAGGAGCCTAAGTTCTTGAAGAATGGTGATGCCGGATTTGTGAAGATGATTCCAACTAAGCCCATGGTTGTCGAGACCTTCTCTGAGTACCCACCACTCGGACGTTTTGCTGTGAGGGACATGCGCCAGACTGTTGCAGTTGGTGTCATCAAGAGCGTTGAGAAGAAGGATCCAACTGGAGCTAAGATCACCAAGGCCGCCGCTAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

448

Amino Acids

49.43

Weight (kDa)

9.14

Isoelectric Point (pI)

30.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 223 1.5e-58 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 248 - 313 3.1e-16 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 322 - 429 4.7e-27 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 327 - 427 6.4e-37 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 743
AccI GTMKAC 1 cut(s) 48
AciI CCGC 2 cut(s) 1326, 1329
AclWI GGATC 4 cut(s) 927, 953, 1292, 1305
AcoI YGGCCR 2 cut(s) 40, 73
AcsI RAATTY 1 cut(s) 236
AcuI CTGAAG 1 cut(s) 698
AfaI GTAC 7 cut(s) 254, 485, 500, 632, 725, 745, 1217
AfiI CCNNNNNNNGG 4 cut(s) 581, 582, 652, 1226
AgsI TTSAA 4 cut(s) 163, 371, 895, 1147
AjnI CCWGG 5 cut(s) 170, 277, 321, 784, 874
AloI GAACNNNNNNTCC 2 cut(s) 1124, 1156
AluBI AGCT 7 cut(s) 91, 187, 374, 857, 1087, 1123, 1310
AluI AGCT 7 cut(s) 91, 187, 374, 857, 1087, 1123, 1310
Alw21I GWGCWC 1 cut(s) 1125
Alw26I GTCTC 3 cut(s) 236, 761, 1196
AlwI GGATC 4 cut(s) 927, 953, 1292, 1305
Ama87I CYCGRG 2 cut(s) 1013, 1124
AoxI GGCC 6 cut(s) 40, 73, 683, 805, 867, 1323
ApeKI GCWGC 4 cut(s) 137, 450, 453, 974
ApoI RAATTY 1 cut(s) 236
Asp718I GGTACC 1 cut(s) 743
AspLEI GCGC 1 cut(s) 1254
AspS9I GGNCC 5 cut(s) 612, 638, 684, 806, 868
AsuC2I CCSGG 2 cut(s) 1014, 1015
AsuHPI GGTGA 5 cut(s) 605, 808, 890, 1166, 1309
AvaI CYCGRG 2 cut(s) 1013, 1124
AvaII GGWCC 2 cut(s) 612, 638
BaeI ACNNNNGTAYC 2 cut(s) 622, 655
BalI TGGCCA 2 cut(s) 42, 75
BamHI GGATCC 1 cut(s) 1297
BanI GGYRCC 1 cut(s) 743
BanII GRGCYC 2 cut(s) 678, 1125
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
Bbv12I GWGCWC 1 cut(s) 1125
BbvI GCAGC 4 cut(s) 124, 437, 440, 961
BccI CCATC 3 cut(s) 344, 383, 457
BcgI CGANNNNNNTGC 2 cut(s) 1105, 1139
BciT130I CCWGG 5 cut(s) 172, 279, 323, 786, 876
BcnI CCSGG 2 cut(s) 1014, 1015
BcoDI GTCTC 3 cut(s) 236, 761, 1196
BglI GCCNNNNNGGC 2 cut(s) 682, 703
BisI GCNGC 6 cut(s) 138, 451, 454, 975, 1326, 1329
BlpI GCTNAGC 2 cut(s) 195, 1083
BlsI GCNGC 6 cut(s) 139, 452, 455, 976, 1327, 1330
BmcAI AGTACT 2 cut(s) 254, 485
Bme1390I CCNGG 7 cut(s) 172, 279, 323, 786, 876, 1014, 1015
Bme18I GGWCC 2 cut(s) 612, 638
BmeT110I CYCGRG 2 cut(s) 1013, 1124
BmgT120I GGNCC 5 cut(s) 612, 638, 684, 806, 868
BmiI GGNNCC 6 cut(s) 316, 639, 640, 745, 1134, 1299
BmrFI CCNGG 7 cut(s) 172, 279, 323, 786, 876, 1014, 1015
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 4 cut(s) 262, 457, 834, 1147
BmuI ACTGGG 1 cut(s) 822
BoxI GACNNNNGTC 1 cut(s) 773
BplI GAGNNNNNCTC 2 cut(s) 1193, 1225
BpmI CTGGAG 3 cut(s) 381, 884, 1326
Bpu1102I GCTNAGC 2 cut(s) 195, 1083
BpuEI CTTGAG 4 cut(s) 173, 518, 671, 908
BpuMI CCSGG 2 cut(s) 1014, 1015
BsaI GGTCTC 2 cut(s) 236, 1196
BsaJI CCNNGG 8 cut(s) 171, 321, 384, 951, 1012, 1013, 1191, 1320
BsaXI ACNNNNNCTCC 2 cut(s) 1124, 1154
Bsc4I CCNNNNNNNGG 4 cut(s) 581, 582, 652, 1226
Bse1I ACTGG 7 cut(s) 76, 364, 632, 775, 817, 1040, 1309
Bse3DI GCAATG 1 cut(s) 1065
BseBI CCWGG 5 cut(s) 172, 279, 323, 786, 876
BseDI CCNNGG 8 cut(s) 171, 321, 384, 951, 1012, 1013, 1191, 1320
BseGI GGATG 5 cut(s) 336, 394, 472, 724, 961
BseLI CCNNNNNNNGG 4 cut(s) 581, 582, 652, 1226
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 7 cut(s) 132, 186, 702, 816, 1004, 1074, 1203
BseNI ACTGG 7 cut(s) 76, 364, 632, 775, 817, 1040, 1309
BseXI GCAGC 4 cut(s) 124, 437, 440, 961
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 6 cut(s) 42, 75, 685, 807, 869, 1325
BshNI GGYRCC 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 1125
BsiHKCI CYCGRG 2 cut(s) 1013, 1124
BsiSI CCGG 3 cut(s) 703, 1014, 1161
BslFI GGGAC 4 cut(s) 557, 624, 734, 1259
BslI CCNNNNNNNGG 4 cut(s) 581, 582, 652, 1226
BsmAI GTCTC 3 cut(s) 236, 761, 1196
BsmFI GGGAC 4 cut(s) 557, 624, 734, 1259
BsnI GGCC 6 cut(s) 42, 75, 685, 807, 869, 1325
Bso31I GGTCTC 2 cut(s) 236, 1196
BsoBI CYCGRG 2 cut(s) 1013, 1124
Bsp1286I GDGCHC 2 cut(s) 678, 1125
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 8 cut(s) 81, 114, 666, 919, 958, 1109, 1297, 1314
Bsp1720I GCTNAGC 2 cut(s) 195, 1083
Bsp19I CCATGG 1 cut(s) 1191
BspACI CCGC 2 cut(s) 1326, 1329
BspANI GGCC 6 cut(s) 42, 75, 685, 807, 869, 1325
BspCNI CTCAG 7 cut(s) 133, 187, 701, 817, 1003, 1075, 1204
BspHI TCATGA 1 cut(s) 1002
BspLI GGNNCC 6 cut(s) 316, 639, 640, 745, 1134, 1299
BspPI GGATC 4 cut(s) 927, 953, 1292, 1305
BspT107I GGYRCC 1 cut(s) 743
BspTNI GGTCTC 2 cut(s) 236, 1196
BsrDI GCAATG 1 cut(s) 1065
BsrGI TGTACA 1 cut(s) 723
BsrI ACTGG 7 cut(s) 76, 364, 632, 775, 817, 1040, 1309
BssECI CCNNGG 8 cut(s) 171, 321, 384, 951, 1012, 1013, 1191, 1320
BssMI GATC 8 cut(s) 81, 114, 666, 919, 958, 1109, 1297, 1314
BssT1I CCWWGG 4 cut(s) 384, 951, 1191, 1320
Bst2UI CCWGG 5 cut(s) 172, 279, 323, 786, 876
Bst4CI ACNGT 5 cut(s) 265, 332, 748, 1052, 1261
BstAPI GCANNNNNTGC 1 cut(s) 412
BstAUI TGTACA 1 cut(s) 723
BstC8I GCNNGC 2 cut(s) 408, 417
BstDSI CCRYGG 1 cut(s) 1191
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 5 cut(s) 336, 394, 472, 724, 961
BstHHI GCGC 1 cut(s) 1254
BstKTI GATC 8 cut(s) 84, 117, 669, 922, 961, 1112, 1300, 1317
BstMAI GTCTC 3 cut(s) 236, 761, 1196
BstMBI GATC 8 cut(s) 81, 114, 666, 919, 958, 1109, 1297, 1314
BstMWI GCNNNNNNNGC 6 cut(s) 332, 412, 416, 682, 703, 971
BstNI CCWGG 5 cut(s) 172, 279, 323, 786, 876
BstNSI RCATGY 2 cut(s) 410, 1252
BstPAI GACNNNNGTC 1 cut(s) 773
BstSCI CCNGG 7 cut(s) 170, 277, 321, 784, 874, 1012, 1013
BstV1I GCAGC 4 cut(s) 124, 437, 440, 961
BstX2I RGATCY 2 cut(s) 919, 1297
BstYI RGATCY 2 cut(s) 919, 1297
BsuRI GGCC 6 cut(s) 42, 75, 685, 807, 869, 1325
BtgI CCRYGG 1 cut(s) 1191
BtsCI GGATG 5 cut(s) 336, 394, 472, 724, 961
BtsIMutI CAGTG 3 cut(s) 69, 261, 357
Cac8I GCNNGC 2 cut(s) 408, 417
CciI TCATGA 1 cut(s) 1002
CfoI GCGC 1 cut(s) 1254
Cfr13I GGNCC 5 cut(s) 612, 638, 684, 806, 868
Cfr9I CCCGGG 1 cut(s) 1013
Csp6I GTAC 7 cut(s) 253, 484, 499, 631, 724, 744, 1216
CspCI CAANNNNNGTGG 8 cut(s) 76, 111, 604, 631, 639, 666, 715, 750
CviAII CATG 7 cut(s) 44, 304, 407, 601, 1003, 1192, 1249
CviQI GTAC 7 cut(s) 253, 484, 499, 631, 724, 744, 1216
DpnI GATC 8 cut(s) 83, 116, 668, 921, 960, 1111, 1299, 1316
DpnII GATC 8 cut(s) 81, 114, 666, 919, 958, 1109, 1297, 1314
EaeI YGGCCR 2 cut(s) 40, 73
Ecl136II GAGCTC 1 cut(s) 1123
Eco130I CCWWGG 4 cut(s) 384, 951, 1191, 1320
Eco24I GRGCYC 2 cut(s) 678, 1125
Eco31I GGTCTC 2 cut(s) 236, 1196
Eco47I GGWCC 2 cut(s) 612, 638
Eco53kI GAGCTC 1 cut(s) 1123
Eco57I CTGAAG 1 cut(s) 698
Eco88I CYCGRG 2 cut(s) 1013, 1124
EcoICRI GAGCTC 1 cut(s) 1123
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO109I RGGNCCY 3 cut(s) 638, 684, 868
EcoRII CCWGG 5 cut(s) 170, 277, 321, 784, 874
EcoT14I CCWWGG 4 cut(s) 384, 951, 1191, 1320
EcoT38I GRGCYC 2 cut(s) 678, 1125
ErhI CCWWGG 4 cut(s) 384, 951, 1191, 1320
FaeI CATG 7 cut(s) 47, 307, 410, 604, 1006, 1195, 1252
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 4 cut(s) 557, 624, 734, 1259
FatI CATG 7 cut(s) 43, 303, 406, 600, 1002, 1191, 1248
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 6 cut(s) 138, 451, 454, 975, 1326, 1329
FokI GGATG 5 cut(s) 323, 401, 479, 731, 968
FriOI GRGCYC 2 cut(s) 678, 1125
Fsp4HI GCNGC 6 cut(s) 138, 451, 454, 975, 1326, 1329
GlaI GCGC 1 cut(s) 1253
GluI GCNGC 6 cut(s) 138, 451, 454, 975, 1326, 1329
GsuI CTGGAG 3 cut(s) 381, 884, 1326
HaeIII GGCC 6 cut(s) 42, 75, 685, 807, 869, 1325
HapII CCGG 3 cut(s) 703, 1014, 1161
HhaI GCGC 1 cut(s) 1254
Hin1II CATG 7 cut(s) 47, 307, 410, 604, 1006, 1195, 1252
Hin6I GCGC 1 cut(s) 1252
HinP1I GCGC 1 cut(s) 1252
HincII GTYRAC 2 cut(s) 49, 63
HindII GTYRAC 2 cut(s) 49, 63
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 5 cut(s) 50, 350, 562, 891, 1177
HpaII CCGG 3 cut(s) 703, 1014, 1161
HphI GGTGA 5 cut(s) 605, 808, 890, 1166, 1309
Hpy166II GTNNAC 4 cut(s) 49, 63, 615, 724
Hpy188I TCNGA 4 cut(s) 396, 691, 1213, 1229
Hpy8I GTNNAC 4 cut(s) 49, 63, 615, 724
HpyAV CCTTC 8 cut(s) 124, 508, 532, 656, 881, 1123, 1216, 1288
HpyCH4III ACNGT 5 cut(s) 265, 332, 748, 1052, 1261
HpyCH4IV ACGT 2 cut(s) 897, 1231
HpyCH4V TGCA 6 cut(s) 261, 456, 847, 965, 1037, 1265
HpyF10VI GCNNNNNNNGC 6 cut(s) 332, 412, 416, 682, 703, 971
HpySE526I ACGT 2 cut(s) 897, 1231
Hsp92II CATG 7 cut(s) 47, 307, 410, 604, 1006, 1195, 1252
HspAI GCGC 1 cut(s) 1252
KflI GGGWCCC 1 cut(s) 638
KpnI GGTACC 1 cut(s) 747
Kzo9I GATC 8 cut(s) 81, 114, 666, 919, 958, 1109, 1297, 1314
LmnI GCTCC 4 cut(s) 280, 1120, 1132, 1307
Lsp1109I GCAGC 4 cut(s) 124, 437, 440, 961
LweI GCATC 4 cut(s) 262, 457, 834, 1147
MaeII ACGT 2 cut(s) 897, 1231
MaeIII GTNAC 4 cut(s) 287, 593, 796, 1090
MalI GATC 8 cut(s) 83, 116, 668, 921, 960, 1111, 1299, 1316
MboI GATC 8 cut(s) 81, 114, 666, 919, 958, 1109, 1297, 1314
MboII GAAGA 5 cut(s) 515, 913, 1159, 1183, 1303
MflI RGATCY 2 cut(s) 919, 1297
MhlI GDGCHC 2 cut(s) 678, 1125
MlsI TGGCCA 2 cut(s) 42, 75
MluCI AATT 3 cut(s) 210, 236, 507
MluNI TGGCCA 2 cut(s) 42, 75
MlyI GAGTC 2 cut(s) 44, 344
MmeI TCCRAC 6 cut(s) 159, 736, 868, 969, 1205, 1325
Mox20I TGGCCA 2 cut(s) 42, 75
MscI TGGCCA 2 cut(s) 42, 75
MseI TTAA 1 cut(s) 780
MslI CAYNNNNRTG 1 cut(s) 1001
Msp20I TGGCCA 2 cut(s) 42, 75
MspI CCGG 3 cut(s) 703, 1014, 1161
MspR9I CCNGG 7 cut(s) 172, 279, 323, 786, 876, 1014, 1015
MvaI CCWGG 5 cut(s) 172, 279, 323, 786, 876
MwoI GCNNNNNNNGC 6 cut(s) 332, 412, 416, 682, 703, 971
NciI CCSGG 2 cut(s) 1014, 1015
NcoI CCATGG 1 cut(s) 1191
NdeII GATC 8 cut(s) 81, 114, 666, 919, 958, 1109, 1297, 1314
NlaIII CATG 7 cut(s) 47, 307, 410, 604, 1006, 1195, 1252
NlaIV GGNNCC 6 cut(s) 316, 639, 640, 745, 1134, 1299
NmuCI GTSAC 4 cut(s) 287, 593, 796, 1090
NspI RCATGY 2 cut(s) 410, 1252
PaeI GCATGC 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 1124
PagI TCATGA 1 cut(s) 1002
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 3 cut(s) 562, 891, 1177
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 6 cut(s) 139, 452, 455, 976, 1327, 1330
PleI GAGTC 2 cut(s) 44, 344
PpsI GAGTC 2 cut(s) 44, 344
PpuMI RGGWCCY 1 cut(s) 638
PshAI GACNNNNGTC 1 cut(s) 773
Psp124BI GAGCTC 1 cut(s) 1125
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 5 cut(s) 170, 277, 321, 784, 874
PspGI CCWGG 5 cut(s) 170, 277, 321, 784, 874
PspN4I GGNNCC 6 cut(s) 316, 639, 640, 745, 1134, 1299
PspPI GGNCC 5 cut(s) 612, 638, 684, 806, 868
PspPPI RGGWCCY 1 cut(s) 638
PsuI RGATCY 2 cut(s) 919, 1297
RsaI GTAC 7 cut(s) 254, 485, 500, 632, 725, 745, 1217
RsaNI GTAC 7 cut(s) 253, 484, 499, 631, 724, 744, 1216
RseI CAYNNNNRTG 1 cut(s) 1001
SacI GAGCTC 1 cut(s) 1125
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 780
SatI GCNGC 6 cut(s) 138, 451, 454, 975, 1326, 1329
Sau3AI GATC 8 cut(s) 81, 114, 666, 919, 958, 1109, 1297, 1314
Sau96I GGNCC 5 cut(s) 612, 638, 684, 806, 868
ScaI AGTACT 2 cut(s) 254, 485
SchI GAGTC 2 cut(s) 44, 344
ScrFI CCNGG 7 cut(s) 172, 279, 323, 786, 876, 1014, 1015
SduI GDGCHC 2 cut(s) 678, 1125
SfaNI GCATC 4 cut(s) 262, 457, 834, 1147
Sfr274I CTCGAG 1 cut(s) 1124
SinI GGWCC 2 cut(s) 612, 638
SlaI CTCGAG 1 cut(s) 1124
SmaI CCCGGG 1 cut(s) 1015
SmiMI CAYNNNNRTG 1 cut(s) 1001
SmlI CTYRAG 5 cut(s) 188, 533, 650, 923, 1124
SmoI CTYRAG 5 cut(s) 188, 533, 650, 923, 1124
SphI GCATGC 1 cut(s) 410
Sse9I AATT 3 cut(s) 210, 236, 507
SsiI CCGC 2 cut(s) 1326, 1329
SstI GAGCTC 1 cut(s) 1125
StyD4I CCNGG 7 cut(s) 170, 277, 321, 784, 874, 1012, 1013
StyI CCWWGG 4 cut(s) 384, 951, 1191, 1320
TaaI ACNGT 5 cut(s) 265, 332, 748, 1052, 1261
TaiI ACGT 2 cut(s) 900, 1234
TaqI TCGA 6 cut(s) 48, 117, 126, 1047, 1125, 1200
TasI AATT 3 cut(s) 210, 236, 507
TatI WGTACW 3 cut(s) 252, 483, 723
TauI GCSGC 2 cut(s) 1328, 1331
TfiI GAWTC 3 cut(s) 562, 891, 1177
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TscAI CASTG 3 cut(s) 76, 268, 364
TseFI GTSAC 4 cut(s) 287, 593, 796, 1090
TseI GCWGC 4 cut(s) 137, 450, 453, 974
Tsp45I GTSAC 4 cut(s) 287, 593, 796, 1090
TspDTI ATGAA 2 cut(s) 161, 1019
TspMI CCCGGG 1 cut(s) 1013
TspRI CASTG 3 cut(s) 76, 268, 364
VpaK11BI GGWCC 2 cut(s) 612, 638
XagI CCTNNNNNAGG 1 cut(s) 650
XapI RAATTY 1 cut(s) 236
XceI RCATGY 2 cut(s) 410, 1252
XcmI CCANNNNNNNNNTGG 1 cut(s) 50
XhoI CTCGAG 1 cut(s) 1124
XmaI CCCGGG 1 cut(s) 1013
XmiI GTMKAC 1 cut(s) 48
ZrmI AGTACT 2 cut(s) 254, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.