RchiOBHm_Chr1g0364011

Elongation factor Tu C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000682
Physical Location & Seq
Reverse (-)
55267023 .. 55269683
2661 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58871

Sequence Viewer

Length: 1344 bp
ATGGGCAAGGAAAAGTTTCATATCAACATTGTGGTTATTGGCCATGTTGACTCTGGGAAGTCGACCACCACCGGTCATTTGATCTACAAGCTTGGGGGTATTGACAAGCGTGTGATTGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGCGTTCATTCAAGTATGCTTGGGTGCTTGACAAGCTGAAGGCTGAGCGTGAAAGGGGTATTACCATTGACATTGCTCTCTGGAAGTTTGAGACCACCAAGTACTACTGCACTGTCATTGATGCTCCTGGACATCGTGACTTCATCAAGAACATGATTACTGGAACTTCCCAGGCTGATTGTGCTGTCCTCATTATTGACTCTACCACTGGAGGTTTTGAGGCTGGTATCTCTAAGGATGGTCAGACCCGTGAGCATGCTCTCCTTGCTTTCACCCTTGGTGTTAGGCAAATGATTTGTTGCTGCAACAAGATGGATGCTACCAGTCCTAAGTACTCGAAGGCAAGGTATGATGAAATCGTGAAGGAAGTGTCATCCTATCTGAAGAAGATTGGATACAACCCAGACAAAATTGCTTTTGTTCCCATCTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTCGACTGGTACAAGGGTCCAACCCTTCTTGAGGCCCTTGACATGATCAACGAGCCCAAGAGGCCCTCCGACAAACCCCTCCGACTTCCACTTCAGGATGTCTACAAGATTGGAGGTATTGGAACTGTTCCTGTTGGACGTGTTGAGACTGGTATTATCAAGCCTGGTATGGTTGTGACTTTTGCTCCCACTGGATTGACCACTGAAGTTAAGTCTGTGGAGATGCACCACGAGGCTCTCCAGGAGGCTCTTCCCGGTGACAATGTTGGCTTCAACGTCAAGAACGTTGCAGTCAAGGATCTCAAGCGTGGGTATGTTGCTTCAAACTCCAAGGATGATCCAGCCAAGGAAGCAGCTAACTTCACTGCCCAGGTCATCATTATGAATCACCCTGGCCAGATTGGAAACGGTTATGCTCCTGTGCTTGATTGCCACACCTCTCACATTGCTGTCAAGTTCAATGAGATTTTGACCAAGATTGACAGACGTTCTGGCAAGGAGCTAGAGAAGGAGCCCAAGTTTTTGAAGAATGGTGACGCAGGGTTTGTTAAGATGATTCCCACCAAGCCCATGGTCGTTGAAACTTTCTCTGAGTATCCCCCACTTGGTCGTTTTGCTGTGAGGGACATGCGCCAGACGGTTGCTGTGGGAGTCATCAAGGCTGTGGAGAAGAAGGATCCCAGCGGAGCCAAGGTTACCAAGTCTGCAGCCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

447

Amino Acids

49.43

Weight (kDa)

9.15

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 7 - 221 3.6e-57 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 248 - 313 2.6e-17 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 322 - 429 2e-27 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 327 - 427 5e-38 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 62, 723
AciI CCGC 1 cut(s) 1305
AclI AACGTT 1 cut(s) 906
AclWI GGATC 4 cut(s) 927, 953, 1292, 1305
AcoI YGGCCR 2 cut(s) 40, 1015
AcuI CTGAAG 4 cut(s) 209, 554, 698, 846
AfaI GTAC 3 cut(s) 254, 485, 632
AfiI CCNNNNNNNGG 3 cut(s) 581, 652, 1226
AflIII ACRYGT 1 cut(s) 760
AgeI ACCGGT 1 cut(s) 71
AgsI TTSAA 6 cut(s) 163, 895, 945, 1081, 1147, 1202
AjiI CACGTC 1 cut(s) 761
AjnI CCWGG 6 cut(s) 277, 321, 784, 861, 990, 1012
AluBI AGCT 4 cut(s) 91, 187, 977, 1123
AluI AGCT 4 cut(s) 91, 187, 977, 1123
Alw26I GTCTC 2 cut(s) 236, 761
AlwI GGATC 4 cut(s) 927, 953, 1292, 1305
AoxI GGCC 4 cut(s) 40, 654, 683, 1015
ApeKI GCWGC 4 cut(s) 137, 453, 974, 1328
ArsI GACNNNNNNTTYG 2 cut(s) 1148, 1180
AsiGI ACCGGT 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 15
AspLEI GCGC 1 cut(s) 1254
AspS9I GGNCC 4 cut(s) 612, 638, 655, 684
AsuC2I CCSGG 1 cut(s) 876
AsuHPI GGTGA 5 cut(s) 415, 605, 890, 1001, 1166
AvaII GGWCC 2 cut(s) 612, 638
BaeI ACNNNNGTAYC 2 cut(s) 622, 655
BalI TGGCCA 2 cut(s) 42, 1017
BamHI GGATCC 1 cut(s) 1297
BanII GRGCYC 2 cut(s) 678, 1137
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BauI CACGAG 1 cut(s) 851
BbvI GCAGC 4 cut(s) 124, 440, 986, 1340
BccI CCATC 3 cut(s) 383, 457, 584
BciT130I CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
BciVI GTATCC 2 cut(s) 539, 1227
BclI TGATCA 1 cut(s) 666
BcnI CCSGG 1 cut(s) 876
BcoDI GTCTC 2 cut(s) 236, 761
BfaI CTAG 1 cut(s) 1124
BfmI CTRYAG 1 cut(s) 1326
BfuI GTATCC 2 cut(s) 539, 1227
BglI GCCNNNNNGGC 1 cut(s) 682
BisI GCNGC 4 cut(s) 138, 454, 975, 1329
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 4 cut(s) 139, 455, 976, 1330
BmcAI AGTACT 2 cut(s) 254, 485
Bme1390I CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
Bme18I GGWCC 2 cut(s) 612, 638
BmgBI CACGTC 1 cut(s) 761
BmgT120I GGNCC 4 cut(s) 612, 638, 655, 684
BmiI GGNNCC 4 cut(s) 639, 1134, 1299, 1309
BmrFI CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
BmsI GCATC 3 cut(s) 262, 457, 834
BpmI CTGGAG 2 cut(s) 381, 845
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 2 cut(s) 671, 908
BpuMI CCSGG 1 cut(s) 876
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 8 cut(s) 321, 427, 951, 966, 990, 1012, 1191, 1311
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 6 cut(s) 790, 820, 1124, 1154, 1299, 1329
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 652, 1226
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 6 cut(s) 316, 364, 474, 632, 775, 817
Bse3DI GCAATG 2 cut(s) 222, 1065
BseBI CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
BseDI CCNNGG 8 cut(s) 321, 427, 951, 966, 990, 1012, 1191, 1311
BseGI GGATG 5 cut(s) 394, 472, 524, 724, 961
BseLI CCNNNNNNNGG 3 cut(s) 581, 652, 1226
BseMI GCAATG 2 cut(s) 222, 1065
BseMII CTCAG 3 cut(s) 132, 186, 1203
BseNI ACTGG 6 cut(s) 316, 364, 474, 632, 775, 817
BseXI GCAGC 4 cut(s) 124, 440, 986, 1340
BseYI CCCAGC 1 cut(s) 1301
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 4 cut(s) 42, 656, 685, 1017
BshTI ACCGGT 1 cut(s) 71
BsiSI CCGG 2 cut(s) 72, 876
BslFI GGGAC 1 cut(s) 1259
BslI CCNNNNNNNGG 3 cut(s) 581, 652, 1226
BsmAI GTCTC 2 cut(s) 236, 761
BsmFI GGGAC 1 cut(s) 1259
BsnI GGCC 4 cut(s) 42, 656, 685, 1017
Bso31I GGTCTC 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 678, 1137
Bsp143I GATC 5 cut(s) 81, 666, 919, 958, 1297
Bsp1720I GCTNAGC 1 cut(s) 195
Bsp19I CCATGG 1 cut(s) 1191
BspACI CCGC 1 cut(s) 1305
BspANI GGCC 4 cut(s) 42, 656, 685, 1017
BspCNI CTCAG 3 cut(s) 133, 187, 1204
BspLI GGNNCC 4 cut(s) 639, 1134, 1299, 1309
BspMAI CTGCAG 1 cut(s) 1330
BspPI GGATC 4 cut(s) 927, 953, 1292, 1305
BspQI GCTCTTC 1 cut(s) 876
BspTNI GGTCTC 1 cut(s) 236
BsrDI GCAATG 2 cut(s) 222, 1065
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 6 cut(s) 316, 364, 474, 632, 775, 817
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 8 cut(s) 321, 427, 951, 966, 990, 1012, 1191, 1311
BssMI GATC 5 cut(s) 81, 666, 919, 958, 1297
BssSI CACGAG 1 cut(s) 851
BssT1I CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
Bst2BI CACGAG 1 cut(s) 851
Bst2UI CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
Bst4CI ACNGT 4 cut(s) 265, 748, 1031, 1261
Bst6I CTCTTC 1 cut(s) 876
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 5 cut(s) 141, 195, 384, 480, 1212
BstDSI CCRYGG 1 cut(s) 1191
BstEII GGTNACC 1 cut(s) 1315
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 5 cut(s) 394, 472, 524, 724, 961
BstHHI GCGC 1 cut(s) 1254
BstKTI GATC 5 cut(s) 84, 669, 922, 961, 1300
BstMAI GTCTC 2 cut(s) 236, 761
BstMBI GATC 5 cut(s) 81, 666, 919, 958, 1297
BstMWI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
BstNI CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
BstNSI RCATGY 2 cut(s) 410, 1252
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
BstSFI CTRYAG 1 cut(s) 1326
BstV1I GCAGC 4 cut(s) 124, 440, 986, 1340
BstX2I RGATCY 2 cut(s) 919, 1297
BstXI CCANNNNNNTGG 1 cut(s) 1192
BstYI RGATCY 2 cut(s) 919, 1297
BsuI GTATCC 2 cut(s) 539, 1227
BsuRI GGCC 4 cut(s) 42, 656, 685, 1017
BtgI CCRYGG 1 cut(s) 1191
BtrI CACGTC 1 cut(s) 761
BtsCI GGATG 5 cut(s) 394, 472, 524, 724, 961
BtsI GCAGTG 1 cut(s) 984
BtsIMutI CAGTG 5 cut(s) 261, 357, 810, 822, 984
Cac8I GCNNGC 1 cut(s) 408
CfoI GCGC 1 cut(s) 1254
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 4 cut(s) 612, 638, 655, 684
CseI GACGC 1 cut(s) 1166
Csp6I GTAC 3 cut(s) 253, 484, 631
CspAI ACCGGT 1 cut(s) 71
CviAII CATG 7 cut(s) 44, 304, 407, 601, 664, 1192, 1249
CviQI GTAC 3 cut(s) 253, 484, 631
DdeI CTNAG 5 cut(s) 141, 195, 384, 480, 1212
DpnI GATC 5 cut(s) 83, 668, 921, 960, 1299
DpnII GATC 5 cut(s) 81, 666, 919, 958, 1297
EaeI YGGCCR 2 cut(s) 40, 1015
Eam1104I CTCTTC 1 cut(s) 876
EarI CTCTTC 1 cut(s) 876
Eco130I CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
Eco24I GRGCYC 2 cut(s) 678, 1137
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 2 cut(s) 612, 638
Eco57I CTGAAG 4 cut(s) 209, 554, 698, 846
Eco91I GGTNACC 1 cut(s) 1315
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO109I RGGNCCY 2 cut(s) 655, 684
EcoO65I GGTNACC 1 cut(s) 1315
EcoRII CCWGG 6 cut(s) 277, 321, 784, 861, 990, 1012
EcoT14I CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
EcoT38I GRGCYC 2 cut(s) 678, 1137
ErhI CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
FaeI CATG 7 cut(s) 47, 307, 410, 604, 667, 1195, 1252
FaqI GGGAC 1 cut(s) 1259
FatI CATG 7 cut(s) 43, 303, 406, 600, 663, 1191, 1248
FbaI TGATCA 1 cut(s) 666
FblI GTMKAC 2 cut(s) 62, 723
Fnu4HI GCNGC 4 cut(s) 138, 454, 975, 1329
FokI GGATG 5 cut(s) 401, 479, 511, 731, 968
FriOI GRGCYC 2 cut(s) 678, 1137
Fsp4HI GCNGC 4 cut(s) 138, 454, 975, 1329
FspBI CTAG 1 cut(s) 1124
GlaI GCGC 1 cut(s) 1253
GluI GCNGC 4 cut(s) 138, 454, 975, 1329
GsaI CCCAGC 1 cut(s) 1305
GsuI CTGGAG 2 cut(s) 381, 845
HaeIII GGCC 4 cut(s) 42, 656, 685, 1017
HapII CCGG 2 cut(s) 72, 876
HgaI GACGC 1 cut(s) 1166
HhaI GCGC 1 cut(s) 1254
Hin1II CATG 7 cut(s) 47, 307, 410, 604, 667, 1195, 1252
Hin6I GCGC 1 cut(s) 1252
HinP1I GCGC 1 cut(s) 1252
HincII GTYRAC 2 cut(s) 49, 63
HindII GTYRAC 2 cut(s) 49, 63
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 5 cut(s) 50, 350, 1006, 1177, 1272
HpaII CCGG 2 cut(s) 72, 876
HphI GGTGA 5 cut(s) 415, 605, 890, 1001, 1166
Hpy166II GTNNAC 4 cut(s) 49, 63, 615, 724
Hpy188I TCNGA 5 cut(s) 396, 534, 691, 704, 1213
Hpy188III TCNNGA 9 cut(s) 127, 232, 287, 298, 511, 582, 650, 716, 901
Hpy8I GTNNAC 4 cut(s) 49, 63, 615, 724
HpyAV CCTTC 7 cut(s) 124, 184, 484, 508, 656, 1123, 1288
HpyCH4III ACNGT 4 cut(s) 265, 748, 1031, 1261
HpyCH4IV ACGT 4 cut(s) 760, 897, 906, 1108
HpyCH4V TGCA 5 cut(s) 261, 456, 847, 911, 1328
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
HpyF3I CTNAG 5 cut(s) 141, 195, 384, 480, 1212
HpySE526I ACGT 4 cut(s) 760, 897, 906, 1108
Hsp92II CATG 7 cut(s) 47, 307, 410, 604, 667, 1195, 1252
HspAI GCGC 1 cut(s) 1252
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 5 cut(s) 81, 666, 919, 958, 1297
LguI GCTCTTC 1 cut(s) 876
LmnI GCTCC 6 cut(s) 280, 811, 1042, 1120, 1132, 1307
Lsp1109I GCAGC 4 cut(s) 124, 440, 986, 1340
LweI GCATC 3 cut(s) 262, 457, 834
MaeI CTAG 1 cut(s) 1124
MaeII ACGT 4 cut(s) 760, 897, 906, 1108
MaeIII GTNAC 6 cut(s) 287, 593, 796, 878, 1154, 1315
MalI GATC 5 cut(s) 83, 668, 921, 960, 1299
MboI GATC 5 cut(s) 81, 666, 919, 958, 1297
MboII GAAGA 5 cut(s) 547, 550, 863, 1159, 1303
MflI RGATCY 2 cut(s) 919, 1297
MhlI GDGCHC 2 cut(s) 678, 1137
MlsI TGGCCA 2 cut(s) 42, 1017
MluCI AATT 1 cut(s) 561
MluNI TGGCCA 2 cut(s) 42, 1017
MlyI GAGTC 3 cut(s) 44, 344, 1281
MmeI TCCRAC 4 cut(s) 665, 714, 727, 736
Mox20I TGGCCA 2 cut(s) 42, 1017
MroXI GAANNNNTTC 1 cut(s) 15
MscI TGGCCA 2 cut(s) 42, 1017
MseI TTAA 2 cut(s) 831, 1170
MslI CAYNNNNRTG 1 cut(s) 1001
Msp20I TGGCCA 2 cut(s) 42, 1017
MspA1I CMGCKG 1 cut(s) 1305
MspI CCGG 2 cut(s) 72, 876
MspR9I CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
MvaI CCWGG 6 cut(s) 279, 323, 786, 863, 992, 1014
MwoI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
NciI CCSGG 1 cut(s) 876
NcoI CCATGG 1 cut(s) 1191
NdeII GATC 5 cut(s) 81, 666, 919, 958, 1297
NlaIII CATG 7 cut(s) 47, 307, 410, 604, 667, 1195, 1252
NlaIV GGNNCC 4 cut(s) 639, 1134, 1299, 1309
NmuCI GTSAC 5 cut(s) 287, 593, 796, 878, 1154
NspI RCATGY 2 cut(s) 410, 1252
PaeI GCATGC 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 876
PcsI WCGNNNNNNNCGW 1 cut(s) 903
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 2 cut(s) 1006, 1177
PfoI TCCNGGA 2 cut(s) 277, 861
PinAI ACCGGT 1 cut(s) 71
PkrI GCNGC 4 cut(s) 139, 455, 976, 1330
PleI GAGTC 3 cut(s) 44, 344, 1280
PpsI GAGTC 3 cut(s) 44, 344, 1280
Psp1406I AACGTT 1 cut(s) 906
Psp6I CCWGG 6 cut(s) 277, 321, 784, 861, 990, 1012
PspEI GGTNACC 1 cut(s) 1315
PspFI CCCAGC 1 cut(s) 1301
PspGI CCWGG 6 cut(s) 277, 321, 784, 861, 990, 1012
PspN4I GGNNCC 4 cut(s) 639, 1134, 1299, 1309
PspPI GGNCC 4 cut(s) 612, 638, 655, 684
PstI CTGCAG 1 cut(s) 1330
PsuI RGATCY 2 cut(s) 919, 1297
RsaI GTAC 3 cut(s) 254, 485, 632
RsaNI GTAC 3 cut(s) 253, 484, 631
RseI CAYNNNNRTG 1 cut(s) 1001
SalI GTCGAC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 876
SaqAI TTAA 2 cut(s) 831, 1170
SatI GCNGC 4 cut(s) 138, 454, 975, 1329
Sau3AI GATC 5 cut(s) 81, 666, 919, 958, 1297
Sau96I GGNCC 4 cut(s) 612, 638, 655, 684
ScaI AGTACT 2 cut(s) 254, 485
SchI GAGTC 3 cut(s) 44, 344, 1281
ScrFI CCNGG 7 cut(s) 279, 323, 786, 863, 876, 992, 1014
SduI GDGCHC 2 cut(s) 678, 1137
SfaNI GCATC 3 cut(s) 262, 457, 834
SfcI CTRYAG 1 cut(s) 1326
SinI GGWCC 2 cut(s) 612, 638
SmiMI CAYNNNNRTG 1 cut(s) 1001
SmlI CTYRAG 2 cut(s) 650, 923
SmoI CTYRAG 2 cut(s) 650, 923
SphI GCATGC 1 cut(s) 410
Sse9I AATT 1 cut(s) 561
SsiI CCGC 1 cut(s) 1305
SspMI CTAG 1 cut(s) 1124
StyD4I CCNGG 7 cut(s) 277, 321, 784, 861, 874, 990, 1012
StyI CCWWGG 5 cut(s) 427, 951, 966, 1191, 1311
TaaI ACNGT 4 cut(s) 265, 748, 1031, 1261
TaiI ACGT 4 cut(s) 763, 900, 909, 1111
TaqI TCGA 4 cut(s) 62, 126, 488, 624
TasI AATT 1 cut(s) 561
TatI WGTACW 2 cut(s) 252, 483
TfiI GAWTC 2 cut(s) 1006, 1177
Tru1I TTAA 2 cut(s) 831, 1170
Tru9I TTAA 2 cut(s) 831, 1170
TscAI CASTG 5 cut(s) 268, 364, 817, 829, 991
TseFI GTSAC 5 cut(s) 287, 593, 796, 878, 1154
TseI GCWGC 4 cut(s) 137, 453, 974, 1328
Tsp45I GTSAC 5 cut(s) 287, 593, 796, 878, 1154
TspDTI ATGAA 6 cut(s) 8, 147, 161, 283, 519, 1019
TspRI CASTG 5 cut(s) 268, 364, 817, 829, 991
VpaK11BI GGWCC 2 cut(s) 612, 638
XagI CCTNNNNNAGG 1 cut(s) 650
XceI RCATGY 2 cut(s) 410, 1252
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 1189
XmiI GTMKAC 2 cut(s) 62, 723
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 1 cut(s) 1124
ZrmI AGTACT 2 cut(s) 254, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.