Rorug05G0330500

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000682
Physical Location & Seq
Forward (+)
39885264 .. 39887898
2635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0330500.1

Sequence Viewer

Length: 855 bp
ATGGACAATGTTAACCTTTTGACAAGTACAGATGCAGATGGTGGACTGCTGGCTTCTCCTCCTATTAGATTTAACAAAGAAGAAATATTGTTAGCTGTCTCAACAAATGACAATGGTATTAAACTTCTAGCAAATTCAGATGGAATTAGGTTACTGAGAACAGTGGATGCTTCCAGAGCTGCTTCTGTGGCTGTTGTGAAGGTCTCAGCAGTAGGAACATTTGGATTGTCTAACATTACTGTTGGAACAGGCATTGGAGATCGAGCAGCTCCAATGCCAGGCATGGTTGGACTGTCTAAACTCTGCATGCGGTCCCAGCGCTGCCTCACCTTCCCTGTCATAGTGTCATATTCCAACGAGCCATTAAAAAAACCAGTATTTGCAGGAAATATAAGGAAAAAGAGCAACGACAGCAGCAGCAACACATGCATTATGGAAAAGGAAACCACTATATTGAAGGCTGAAGAGCTCAATAAGAAGCATCAACAAATCCCTGGCAGCAGACAAGTTCTAGAACTTTTGAAAGCTACTTCAGACCCATCTGCCAATGTTCTCGACATCGTGCTTTACTTTTCAAGGCGTAACGGAGAGATTAGGTGTATTTCAGCAGAGATAAAATTGCACCACCAGCGGCAAGAGGAGGACAAAGTACTAGATGAAGAAATTGAAGAGGATGAGATGTTCTGGAATCAAGAAGCTCTTAAAGAGGAGGAAGAAGATGATAACTATGAAGTGGAGCTGAAGTTGCTGATGTGTTCTCAAATTGTTTACTTTAATTTCAAGTTTTCTAGCATTTTAGAGGAAATGTATCTTTGTATTTCCTTTGATTATGTATTTTACAAACTTAAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

284

Amino Acids

31.83

Weight (kDa)

5.08

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 310, 631
AcsI RAATTY 1 cut(s) 133
AcuI CTGAAG 3 cut(s) 483, 516, 761
AfaI GTAC 2 cut(s) 28, 651
AfeI AGCGCT 1 cut(s) 320
AfiI CCNNNNNNNGG 1 cut(s) 278
AgsI TTSAA 5 cut(s) 457, 523, 576, 668, 781
AjnI CCWGG 2 cut(s) 277, 493
AloI GAACNNNNNNTCC 2 cut(s) 665, 697
AluBI AGCT 7 cut(s) 95, 179, 269, 469, 527, 698, 739
AluI AGCT 7 cut(s) 95, 179, 269, 469, 527, 698, 739
Alw21I GWGCWC 1 cut(s) 471
Alw26I GTCTC 2 cut(s) 103, 208
Aor51HI AGCGCT 1 cut(s) 320
ApeKI GCWGC 6 cut(s) 179, 266, 321, 414, 417, 498
ApoI RAATTY 1 cut(s) 133
AspLEI GCGC 1 cut(s) 321
AspS9I GGNCC 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 1 cut(s) 312
BanII GRGCYC 1 cut(s) 471
Bbv12I GWGCWC 1 cut(s) 471
BbvI GCAGC 6 cut(s) 166, 278, 308, 426, 429, 510
BccI CCATC 3 cut(s) 32, 134, 547
BciT130I CCWGG 2 cut(s) 279, 495
BcoDI GTCTC 2 cut(s) 103, 208
BfaI CTAG 4 cut(s) 128, 512, 653, 789
BfoI RGCGCY 1 cut(s) 322
BisI GCNGC 7 cut(s) 180, 267, 322, 415, 418, 499, 632
BlsI GCNGC 7 cut(s) 181, 268, 323, 416, 419, 500, 633
BmcAI AGTACT 1 cut(s) 651
Bme1390I CCNGG 2 cut(s) 279, 495
Bme18I GGWCC 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 312
BmiI GGNNCC 1 cut(s) 314
BmrFI CCNGG 2 cut(s) 279, 495
BmsI GCATC 3 cut(s) 22, 157, 490
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 1 cut(s) 493
BsaXI ACNNNNNCTCC 2 cut(s) 728, 758
Bsc4I CCNNNNNNNGG 1 cut(s) 278
Bse1I ACTGG 1 cut(s) 374
BseBI CCWGG 2 cut(s) 279, 495
BseDI CCNNGG 1 cut(s) 493
BseGI GGATG 2 cut(s) 172, 679
BseLI CCNNNNNNNGG 1 cut(s) 278
BseMII CTCAG 2 cut(s) 146, 219
BseNI ACTGG 1 cut(s) 374
BseRI GAGGAG 3 cut(s) 48, 653, 722
BseXI GCAGC 6 cut(s) 166, 278, 308, 426, 429, 510
BseYI CCCAGC 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 471
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 1 cut(s) 278
BsmAI GTCTC 2 cut(s) 103, 208
BsmFI GGGAC 1 cut(s) 298
Bso31I GGTCTC 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 471
Bsp143I GATC 1 cut(s) 259
BspACI CCGC 2 cut(s) 310, 631
BspCNI CTCAG 2 cut(s) 147, 218
BspLI GGNNCC 1 cut(s) 314
BspQI GCTCTTC 1 cut(s) 459
BspTNI GGTCTC 1 cut(s) 208
BsrI ACTGG 1 cut(s) 374
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 1 cut(s) 259
Bst2UI CCWGG 2 cut(s) 279, 495
Bst4CI ACNGT 3 cut(s) 163, 241, 294
Bst6I CTCTTC 2 cut(s) 459, 663
BstAPI GCANNNNNTGC 1 cut(s) 426
BstC8I GCNNGC 2 cut(s) 51, 308
BstDEI CTNAG 2 cut(s) 155, 205
BstF5I GGATG 2 cut(s) 172, 679
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 1 cut(s) 262
BstMAI GTCTC 2 cut(s) 103, 208
BstMBI GATC 1 cut(s) 259
BstMWI GCNNNNNNNGC 7 cut(s) 176, 188, 316, 411, 426, 628, 745
BstNI CCWGG 2 cut(s) 279, 495
BstNSI RCATGY 2 cut(s) 310, 429
BstSCI CCNGG 2 cut(s) 277, 493
BstV1I GCAGC 6 cut(s) 166, 278, 308, 426, 429, 510
BtsCI GGATG 2 cut(s) 172, 679
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 2 cut(s) 51, 308
CfoI GCGC 1 cut(s) 321
Cfr13I GGNCC 1 cut(s) 312
Csp6I GTAC 2 cut(s) 27, 650
CviAII CATG 3 cut(s) 283, 307, 426
CviQI GTAC 2 cut(s) 27, 650
DdeI CTNAG 2 cut(s) 155, 205
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
Eam1104I CTCTTC 2 cut(s) 459, 663
EarI CTCTTC 2 cut(s) 459, 663
Ecl136II GAGCTC 1 cut(s) 469
Eco24I GRGCYC 1 cut(s) 471
Eco31I GGTCTC 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 312
Eco47III AGCGCT 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 3 cut(s) 483, 516, 761
EcoICRI GAGCTC 1 cut(s) 469
EcoRII CCWGG 2 cut(s) 277, 493
EcoT22I ATGCAT 1 cut(s) 431
EcoT38I GRGCYC 1 cut(s) 471
FaeI CATG 3 cut(s) 286, 310, 429
FalI AAGNNNNNCTT 2 cut(s) 684, 716
FaqI GGGAC 1 cut(s) 298
FatI CATG 3 cut(s) 282, 306, 425
Fnu4HI GCNGC 7 cut(s) 180, 267, 322, 415, 418, 499, 632
FokI GGATG 2 cut(s) 179, 686
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 7 cut(s) 180, 267, 322, 415, 418, 499, 632
FspBI CTAG 4 cut(s) 128, 512, 653, 789
GlaI GCGC 1 cut(s) 320
GluI GCNGC 7 cut(s) 180, 267, 322, 415, 418, 499, 632
GsaI CCCAGC 1 cut(s) 319
HaeII RGCGCY 1 cut(s) 322
HhaI GCGC 1 cut(s) 321
Hin1II CATG 3 cut(s) 286, 310, 429
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 1 cut(s) 688
HpaI GTTAAC 1 cut(s) 13
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 3 cut(s) 13, 44, 769
Hpy188I TCNGA 2 cut(s) 139, 535
Hpy188III TCNNGA 5 cut(s) 174, 512, 554, 685, 692
Hpy8I GTNNAC 3 cut(s) 13, 44, 769
HpyAV CCTTC 3 cut(s) 193, 340, 451
HpyCH4III ACNGT 3 cut(s) 163, 241, 294
HpyCH4V TGCA 5 cut(s) 35, 306, 383, 429, 622
HpyF10VI GCNNNNNNNGC 7 cut(s) 176, 188, 316, 411, 426, 628, 745
HpyF3I CTNAG 2 cut(s) 155, 205
Hsp92II CATG 3 cut(s) 286, 310, 429
HspAI GCGC 1 cut(s) 319
KspAI GTTAAC 1 cut(s) 13
Kzo9I GATC 1 cut(s) 259
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 2 cut(s) 274, 736
Lsp1109I GCAGC 6 cut(s) 166, 278, 308, 426, 429, 510
LweI GCATC 3 cut(s) 22, 157, 490
MaeI CTAG 4 cut(s) 128, 512, 653, 789
MaeIII GTNAC 2 cut(s) 150, 581
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MboII GAAGA 6 cut(s) 92, 476, 671, 680, 725, 728
MhlI GDGCHC 1 cut(s) 471
MluCI AATT 7 cut(s) 133, 144, 617, 663, 762, 775, 850
MmeI TCCRAC 3 cut(s) 223, 268, 378
MnlI CCTC 8 cut(s) 69, 335, 631, 634, 664, 700, 703, 793
Mph1103I ATGCAT 1 cut(s) 431
MseI TTAA 8 cut(s) 12, 72, 120, 365, 702, 774, 846, 853
MspA1I CMGCKG 1 cut(s) 631
MspR9I CCNGG 2 cut(s) 279, 495
MvaI CCWGG 2 cut(s) 279, 495
MwoI GCNNNNNNNGC 7 cut(s) 176, 188, 316, 411, 426, 628, 745
NdeII GATC 1 cut(s) 259
NlaIII CATG 3 cut(s) 286, 310, 429
NlaIV GGNNCC 1 cut(s) 314
NsiI ATGCAT 1 cut(s) 431
NspI RCATGY 2 cut(s) 310, 429
PaeI GCATGC 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 459
PfeI GAWTC 1 cut(s) 688
PkrI GCNGC 7 cut(s) 181, 268, 323, 416, 419, 500, 633
Psp124BI GAGCTC 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 277, 493
PspFI CCCAGC 1 cut(s) 315
PspGI CCWGG 2 cut(s) 277, 493
PspN4I GGNNCC 1 cut(s) 314
PspPI GGNCC 1 cut(s) 312
RsaI GTAC 2 cut(s) 28, 651
RsaNI GTAC 2 cut(s) 27, 650
SacI GAGCTC 1 cut(s) 471
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 8 cut(s) 12, 72, 120, 365, 702, 774, 846, 853
SatI GCNGC 7 cut(s) 180, 267, 322, 415, 418, 499, 632
Sau3AI GATC 1 cut(s) 259
Sau96I GGNCC 1 cut(s) 312
ScaI AGTACT 1 cut(s) 651
ScrFI CCNGG 2 cut(s) 279, 495
SduI GDGCHC 1 cut(s) 471
SfaNI GCATC 3 cut(s) 22, 157, 490
SinI GGWCC 1 cut(s) 312
SphI GCATGC 1 cut(s) 310
Sse9I AATT 7 cut(s) 133, 144, 617, 663, 762, 775, 850
SsiI CCGC 2 cut(s) 310, 631
SspI AATATT 1 cut(s) 87
SspMI CTAG 4 cut(s) 128, 512, 653, 789
SstI GAGCTC 1 cut(s) 471
StyD4I CCNGG 2 cut(s) 277, 493
TaaI ACNGT 3 cut(s) 163, 241, 294
TaqI TCGA 2 cut(s) 262, 555
TasI AATT 7 cut(s) 133, 144, 617, 663, 762, 775, 850
TatI WGTACW 2 cut(s) 26, 649
TauI GCSGC 1 cut(s) 634
TfiI GAWTC 1 cut(s) 688
Tru1I TTAA 8 cut(s) 12, 72, 120, 365, 702, 774, 846, 853
Tru9I TTAA 8 cut(s) 12, 72, 120, 365, 702, 774, 846, 853
TscAI CASTG 1 cut(s) 168
TseI GCWGC 6 cut(s) 179, 266, 321, 414, 417, 498
TspDTI ATGAA 2 cut(s) 672, 744
TspGWI ACGGA 1 cut(s) 600
TspRI CASTG 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 312
XapI RAATTY 1 cut(s) 133
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 2 cut(s) 310, 429
XspI CTAG 4 cut(s) 128, 512, 653, 789
ZrmI AGTACT 1 cut(s) 651
Zsp2I ATGCAT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.