Rmu_sc0001607.1_g000017

Elongation factor Tu C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001607.1
OG0000682
Physical Location & Seq
Reverse (-)
85000 .. 85929
930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001607.1_g000017.1.cds

Sequence Viewer

Length: 930 bp
atggttgtctttgcttccactaatgttgatggtatttctgttatacagatggatgctaccagtcctaagtactcgaaggcaaggtatgatgaaatcgtgaaggaagtgtcttcctatctgaagaagattggatacaacccagacaaaattgcttttgttcccatctctggatttgagggtgacaacatgattgagaggtccaccaacctcgactggtacaagggtccaacccttcttgaggcccttgacatgatcaacgagcccaagaggccctccgacaagcccctccgacttccacttcaggatgtctacaagattgggggtattggaactgttcctgttggacgtgttgagactggtattatcaagcctggtatggttgtgacttttgctcccactggattgaccactgaagttaagtctgtggagatgcaccacgaggctctccaggaggctcttcccggtgataatgttggcttcaacgtcaagaacgttgcagtcaaggatctcaagcgtgggtatgttgcttcaaactccaaggatgatccagccaaggaagcagctaacttcactgcccaggtcatcattatgaatcaccctggccagattggaaacggttatgctcctgtgcttgattgccacacctctcacattgctgtcaagttcaatgagattttgaccaagattgacagacgttctggcaaggagctagagaaggagcccaagtttttgaagaatggtgacgcagggtttgttaagatgattcccaccaagcccatggtcgttgaaactttctctgagtatcccccacttggtcgttttgctgtgagggacatgcgccagacagttgctgtgggagtcatcaaggctgtggagaagaaggatcccagcggagccaaggttaccaagtctgcagccaagaagaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

309

Amino Acids

33.83

Weight (kDa)

9.17

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 309
AciI CCGC 1 cut(s) 891
AclI AACGTT 1 cut(s) 492
AclWI GGATC 4 cut(s) 513, 539, 878, 891
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 3 cut(s) 140, 284, 432
AfaI GTAC 2 cut(s) 71, 218
AfiI CCNNNNNNNGG 3 cut(s) 167, 238, 812
AflIII ACRYGT 1 cut(s) 346
AgsI TTSAA 5 cut(s) 481, 531, 667, 733, 788
AjiI CACGTC 1 cut(s) 347
AjnI CCWGG 4 cut(s) 370, 447, 576, 598
AluBI AGCT 2 cut(s) 563, 709
AluI AGCT 2 cut(s) 563, 709
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 4 cut(s) 513, 539, 878, 891
AlwNI CAGNNNCTG 1 cut(s) 851
AoxI GGCC 3 cut(s) 240, 269, 601
ApeKI GCWGC 2 cut(s) 560, 914
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
AspLEI GCGC 1 cut(s) 840
AspS9I GGNCC 4 cut(s) 198, 224, 241, 270
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 4 cut(s) 191, 476, 587, 752
AvaII GGWCC 2 cut(s) 198, 224
BaeI ACNNNNGTAYC 2 cut(s) 208, 241
BalI TGGCCA 1 cut(s) 603
BamHI GGATCC 1 cut(s) 883
BanII GRGCYC 2 cut(s) 264, 723
BarI GAAGNNNNNNTAC 2 cut(s) 116, 148
BauI CACGAG 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 572, 926
BccI CCATC 3 cut(s) 23, 43, 170
BciT130I CCWGG 4 cut(s) 372, 449, 578, 600
BciVI GTATCC 2 cut(s) 125, 813
BclI TGATCA 1 cut(s) 252
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 1 cut(s) 347
BfaI CTAG 1 cut(s) 710
BfmI CTRYAG 1 cut(s) 912
BfuI GTATCC 2 cut(s) 125, 813
BglI GCCNNNNNGGC 1 cut(s) 268
BisI GCNGC 2 cut(s) 561, 915
BlsI GCNGC 2 cut(s) 562, 916
BmcAI AGTACT 1 cut(s) 71
Bme1390I CCNGG 5 cut(s) 372, 449, 462, 578, 600
Bme18I GGWCC 2 cut(s) 198, 224
BmgBI CACGTC 1 cut(s) 347
BmgT120I GGNCC 4 cut(s) 198, 224, 241, 270
BmiI GGNNCC 4 cut(s) 225, 720, 885, 895
BmrFI CCNGG 5 cut(s) 372, 449, 462, 578, 600
BmsI GCATC 2 cut(s) 43, 420
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 1 cut(s) 431
BpuEI CTTGAG 2 cut(s) 257, 494
BpuMI CCSGG 1 cut(s) 462
BsaJI CCNNGG 6 cut(s) 537, 552, 576, 598, 777, 897
BsaXI ACNNNNNCTCC 6 cut(s) 376, 406, 710, 740, 885, 915
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 238, 812
Bse1I ACTGG 4 cut(s) 60, 218, 361, 403
Bse3DI GCAATG 1 cut(s) 651
BseBI CCWGG 4 cut(s) 372, 449, 578, 600
BseDI CCNNGG 6 cut(s) 537, 552, 576, 598, 777, 897
BseGI GGATG 3 cut(s) 58, 310, 547
BseLI CCNNNNNNNGG 3 cut(s) 167, 238, 812
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 1 cut(s) 789
BseNI ACTGG 4 cut(s) 60, 218, 361, 403
BseXI GCAGC 2 cut(s) 572, 926
BseYI CCCAGC 1 cut(s) 887
BshFI GGCC 3 cut(s) 242, 271, 603
BsiSI CCGG 1 cut(s) 462
BslFI GGGAC 1 cut(s) 845
BslI CCNNNNNNNGG 3 cut(s) 167, 238, 812
BsmAI GTCTC 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 845
BsnI GGCC 3 cut(s) 242, 271, 603
Bsp1286I GDGCHC 2 cut(s) 264, 723
Bsp143I GATC 4 cut(s) 252, 505, 544, 883
Bsp19I CCATGG 1 cut(s) 777
BspACI CCGC 1 cut(s) 891
BspANI GGCC 3 cut(s) 242, 271, 603
BspCNI CTCAG 1 cut(s) 790
BspLI GGNNCC 4 cut(s) 225, 720, 885, 895
BspMAI CTGCAG 1 cut(s) 916
BspPI GGATC 4 cut(s) 513, 539, 878, 891
BspQI GCTCTTC 1 cut(s) 462
BsrDI GCAATG 1 cut(s) 651
BsrI ACTGG 4 cut(s) 60, 218, 361, 403
BssECI CCNNGG 6 cut(s) 537, 552, 576, 598, 777, 897
BssMI GATC 4 cut(s) 252, 505, 544, 883
BssSI CACGAG 1 cut(s) 437
BssT1I CCWWGG 4 cut(s) 537, 552, 777, 897
Bst2BI CACGAG 1 cut(s) 437
Bst2UI CCWGG 4 cut(s) 372, 449, 578, 600
Bst4CI ACNGT 3 cut(s) 334, 617, 847
Bst6I CTCTTC 1 cut(s) 462
BstDEI CTNAG 2 cut(s) 66, 798
BstDSI CCRYGG 1 cut(s) 777
BstEII GGTNACC 1 cut(s) 901
BstENI CCTNNNNNAGG 1 cut(s) 236
BstF5I GGATG 3 cut(s) 58, 310, 547
BstHHI GCGC 1 cut(s) 840
BstKTI GATC 4 cut(s) 255, 508, 547, 886
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 4 cut(s) 252, 505, 544, 883
BstMWI GCNNNNNNNGC 2 cut(s) 268, 557
BstNI CCWGG 4 cut(s) 372, 449, 578, 600
BstNSI RCATGY 1 cut(s) 838
BstPI GGTNACC 1 cut(s) 901
BstSCI CCNGG 5 cut(s) 370, 447, 460, 576, 598
BstSFI CTRYAG 1 cut(s) 912
BstV1I GCAGC 2 cut(s) 572, 926
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 2 cut(s) 505, 883
BstXI CCANNNNNNTGG 1 cut(s) 778
BstYI RGATCY 2 cut(s) 505, 883
BsuI GTATCC 2 cut(s) 125, 813
BsuRI GGCC 3 cut(s) 242, 271, 603
BtgI CCRYGG 1 cut(s) 777
BtrI CACGTC 1 cut(s) 347
BtsCI GGATG 3 cut(s) 58, 310, 547
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 3 cut(s) 396, 408, 570
CaiI CAGNNNCTG 1 cut(s) 851
CfoI GCGC 1 cut(s) 840
Cfr13I GGNCC 4 cut(s) 198, 224, 241, 270
CseI GACGC 1 cut(s) 752
Csp6I GTAC 2 cut(s) 70, 217
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 4 cut(s) 187, 250, 778, 835
CviQI GTAC 2 cut(s) 70, 217
DdeI CTNAG 2 cut(s) 66, 798
DpnI GATC 4 cut(s) 254, 507, 546, 885
DpnII GATC 4 cut(s) 252, 505, 544, 883
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
Eco130I CCWWGG 4 cut(s) 537, 552, 777, 897
Eco24I GRGCYC 2 cut(s) 264, 723
Eco47I GGWCC 2 cut(s) 198, 224
Eco57I CTGAAG 3 cut(s) 140, 284, 432
Eco91I GGTNACC 1 cut(s) 901
EcoNI CCTNNNNNAGG 1 cut(s) 236
EcoO109I RGGNCCY 2 cut(s) 241, 270
EcoO65I GGTNACC 1 cut(s) 901
EcoRII CCWGG 4 cut(s) 370, 447, 576, 598
EcoT14I CCWWGG 4 cut(s) 537, 552, 777, 897
EcoT38I GRGCYC 2 cut(s) 264, 723
ErhI CCWWGG 4 cut(s) 537, 552, 777, 897
FaeI CATG 4 cut(s) 190, 253, 781, 838
FaqI GGGAC 1 cut(s) 845
FatI CATG 4 cut(s) 186, 249, 777, 834
FbaI TGATCA 1 cut(s) 252
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 2 cut(s) 561, 915
FokI GGATG 3 cut(s) 65, 317, 554
FriOI GRGCYC 2 cut(s) 264, 723
Fsp4HI GCNGC 2 cut(s) 561, 915
FspBI CTAG 1 cut(s) 710
GlaI GCGC 1 cut(s) 839
GluI GCNGC 2 cut(s) 561, 915
GsaI CCCAGC 1 cut(s) 891
GsuI CTGGAG 1 cut(s) 431
HaeIII GGCC 3 cut(s) 242, 271, 603
HapII CCGG 1 cut(s) 462
HgaI GACGC 1 cut(s) 752
HhaI GCGC 1 cut(s) 840
Hin1II CATG 4 cut(s) 190, 253, 781, 838
Hin6I GCGC 1 cut(s) 838
HinP1I GCGC 1 cut(s) 838
HinfI GANTC 3 cut(s) 592, 763, 858
HpaII CCGG 1 cut(s) 462
HphI GGTGA 4 cut(s) 191, 476, 587, 752
Hpy166II GTNNAC 2 cut(s) 201, 310
Hpy188I TCNGA 4 cut(s) 120, 277, 290, 799
Hpy188III TCNNGA 5 cut(s) 97, 168, 236, 302, 487
Hpy8I GTNNAC 2 cut(s) 201, 310
HpyAV CCTTC 5 cut(s) 70, 94, 242, 709, 874
HpyCH4III ACNGT 3 cut(s) 334, 617, 847
HpyCH4IV ACGT 4 cut(s) 346, 483, 492, 694
HpyCH4V TGCA 3 cut(s) 433, 497, 914
HpyF10VI GCNNNNNNNGC 2 cut(s) 268, 557
HpyF3I CTNAG 2 cut(s) 66, 798
HpySE526I ACGT 4 cut(s) 346, 483, 492, 694
Hsp92II CATG 4 cut(s) 190, 253, 781, 838
HspAI GCGC 1 cut(s) 838
Ksp22I TGATCA 1 cut(s) 252
Kzo9I GATC 4 cut(s) 252, 505, 544, 883
LguI GCTCTTC 1 cut(s) 462
LmnI GCTCC 5 cut(s) 397, 628, 706, 718, 893
Lsp1109I GCAGC 2 cut(s) 572, 926
LweI GCATC 2 cut(s) 43, 420
MaeI CTAG 1 cut(s) 710
MaeII ACGT 4 cut(s) 346, 483, 492, 694
MaeIII GTNAC 4 cut(s) 179, 382, 740, 901
MalI GATC 4 cut(s) 254, 507, 546, 885
MboI GATC 4 cut(s) 252, 505, 544, 883
MboII GAAGA 6 cut(s) 102, 133, 136, 449, 745, 889
MflI RGATCY 2 cut(s) 505, 883
MhlI GDGCHC 2 cut(s) 264, 723
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 1 cut(s) 147
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 1 cut(s) 867
MmeI TCCRAC 4 cut(s) 251, 300, 313, 322
Mox20I TGGCCA 1 cut(s) 603
MscI TGGCCA 1 cut(s) 603
MseI TTAA 2 cut(s) 417, 756
MslI CAYNNNNRTG 1 cut(s) 587
Msp20I TGGCCA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 891
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 5 cut(s) 372, 449, 462, 578, 600
MvaI CCWGG 4 cut(s) 372, 449, 578, 600
MwoI GCNNNNNNNGC 2 cut(s) 268, 557
NciI CCSGG 1 cut(s) 462
NcoI CCATGG 1 cut(s) 777
NdeII GATC 4 cut(s) 252, 505, 544, 883
NlaIII CATG 4 cut(s) 190, 253, 781, 838
NlaIV GGNNCC 4 cut(s) 225, 720, 885, 895
NmuCI GTSAC 3 cut(s) 179, 382, 740
NspI RCATGY 1 cut(s) 838
PciSI GCTCTTC 1 cut(s) 462
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PfeI GAWTC 2 cut(s) 592, 763
PfoI TCCNGGA 1 cut(s) 447
PkrI GCNGC 2 cut(s) 562, 916
PleI GAGTC 1 cut(s) 866
PpsI GAGTC 1 cut(s) 866
Psp1406I AACGTT 1 cut(s) 492
Psp6I CCWGG 4 cut(s) 370, 447, 576, 598
PspEI GGTNACC 1 cut(s) 901
PspFI CCCAGC 1 cut(s) 887
PspGI CCWGG 4 cut(s) 370, 447, 576, 598
PspN4I GGNNCC 4 cut(s) 225, 720, 885, 895
PspPI GGNCC 4 cut(s) 198, 224, 241, 270
PstI CTGCAG 1 cut(s) 916
PstNI CAGNNNCTG 1 cut(s) 851
PsuI RGATCY 2 cut(s) 505, 883
RsaI GTAC 2 cut(s) 71, 218
RsaNI GTAC 2 cut(s) 70, 217
RseI CAYNNNNRTG 1 cut(s) 587
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 2 cut(s) 417, 756
SatI GCNGC 2 cut(s) 561, 915
Sau3AI GATC 4 cut(s) 252, 505, 544, 883
Sau96I GGNCC 4 cut(s) 198, 224, 241, 270
ScaI AGTACT 1 cut(s) 71
SchI GAGTC 1 cut(s) 867
ScrFI CCNGG 5 cut(s) 372, 449, 462, 578, 600
SduI GDGCHC 2 cut(s) 264, 723
SfaNI GCATC 2 cut(s) 43, 420
SfcI CTRYAG 1 cut(s) 912
SinI GGWCC 2 cut(s) 198, 224
SmiMI CAYNNNNRTG 1 cut(s) 587
SmlI CTYRAG 2 cut(s) 236, 509
SmoI CTYRAG 2 cut(s) 236, 509
Sse9I AATT 1 cut(s) 147
SsiI CCGC 1 cut(s) 891
SspMI CTAG 1 cut(s) 710
StyD4I CCNGG 5 cut(s) 370, 447, 460, 576, 598
StyI CCWWGG 4 cut(s) 537, 552, 777, 897
TaaI ACNGT 3 cut(s) 334, 617, 847
TaiI ACGT 4 cut(s) 349, 486, 495, 697
TaqI TCGA 2 cut(s) 74, 210
TasI AATT 1 cut(s) 147
TatI WGTACW 1 cut(s) 69
TfiI GAWTC 2 cut(s) 592, 763
Tru1I TTAA 2 cut(s) 417, 756
Tru9I TTAA 2 cut(s) 417, 756
TscAI CASTG 3 cut(s) 403, 415, 577
TseFI GTSAC 3 cut(s) 179, 382, 740
TseI GCWGC 2 cut(s) 560, 914
Tsp45I GTSAC 3 cut(s) 179, 382, 740
TspDTI ATGAA 2 cut(s) 105, 605
TspRI CASTG 3 cut(s) 403, 415, 577
VpaK11BI GGWCC 2 cut(s) 198, 224
XagI CCTNNNNNAGG 1 cut(s) 236
XceI RCATGY 1 cut(s) 838
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 1 cut(s) 710
ZrmI AGTACT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.