Rh4DG186900

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000682
Physical Location & Seq
Forward (+)
36920003 .. 36921912
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG186900.1

Sequence Viewer

Length: 1344 bp
ATGGGTAAGGAGAAGGTTCACATCAACATTGTGGTCATTGGCCATGTCGACTCTGGGAAATCGACCACCACTGGTCATCTCATCTACAAGCTTGGAGGTATTGACAAGCGTGTGATTGAGAGGTTTGAGAAGGAAGCTGCTGAGATGAACAAAAGGTCATTCAAGTACGCCTGGGTGCTCGACAAGCTTAAGGCTGAGCGTGAGCGTGGTATTACCATTGATATTGCTTTGTGGAAGTTTGAGACCACAAGGTACTACTGCACTGTCATTGATGCTCCAGGGCATCGTGACTTTATTAAGAACATGATTACTGGAACTTCACAGGCTGACTGTGCTGTTCTCATTATTGATTCCACCACTGGTGGTTTTGAGGCTGGTATTTCCAAGGATGGGCAGACCCGTGAGCATGCTCTTCTTGCTTTCACCCTTGGTGTGAAGCAAATGATTTGCTGCTGTAACAAGATGGATGCCACTACTCCCAAGTACTCGAAGGCGAGGTACGATGAAATTGTGAAGGAAGTCTCATCCTATTTGAAGAAGGTTGGTTACAACCCTGACAAAATTGCCTTTGTTCCCATCTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGTCCTACCCTTCTTGAGGCACTTGACCAGATCAACGAGCCAAAGAGGCCCTCAGACAAGCCCCTCCGTCTCCCACTGCAGGATGTCTACAAGATTGGTGGTATTGGAACTGTGCCAGTGGGTCGTGTTGAGACTGGAGTTATCAAGCCTGGTATGGTCGTCACTTTTGGTCCAACTGGTCTGACCACTGAAGTCAAGTCTGTTGAGATGCATCATGAGGCTCTTCAGGAGGCTCTCCCTGGTGACAATGTCGGTTTCAATGTTAAGAATGTTGCTGTGAAGGATCTCAAGCGTGGTTATGTTGCATCCAACTCCAAGGATGATCCTGCAAAGGAGGCTGCCAACTTCACATCCCAGGTCATCATCATGAACCACCCTGGACAGATTGGTAATGGTTATGCCCCAGTTCTTGATTGCCACACTTCTCACATTGCTGTGAAGTTCGGTGAAATCCTCACCAAGATTGACAGGAGGTCTGGTAAGGAACTTGAGAAGGAGCCTAAGTTTTTAAAGAATGGAGATGCTGGTATGGTTAAGATGCTTCCCACCAAGCCTATGGTTGTTGAGACCTTCTCTGAGTACCCACCCTTGGGACGTTTTGCTGTCCGTGACATGAGACAGACTGTTGCTGTTGGTGTTATCAAGAACGTGGAGAAGAAGGATCCAAGTGGTGCTAAGGTCACCAAGTCTGCTGCCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

447

Amino Acids

49.34

Weight (kDa)

9.15

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 6 - 223 1.6e-57 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 248 - 313 2.6e-17 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 322 - 429 1e-26 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 327 - 427 9.3e-37 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 48, 723
AclWI GGATC 4 cut(s) 927, 953, 1292, 1305
AcoI YGGCCR 1 cut(s) 40
AcuI CTGAAG 2 cut(s) 845, 846
AfaI GTAC 6 cut(s) 167, 254, 485, 500, 632, 1217
AfiI CCNNNNNNNGG 6 cut(s) 390, 581, 652, 715, 1225, 1226
AflII CTTAAG 1 cut(s) 188
AgsI TTSAA 3 cut(s) 163, 535, 895
AhdI GACNNNNNGTC 1 cut(s) 1108
AjnI CCWGG 6 cut(s) 170, 277, 784, 874, 990, 1012
AluBI AGCT 3 cut(s) 91, 137, 187
AluI AGCT 3 cut(s) 91, 137, 187
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 6 cut(s) 236, 526, 710, 761, 1196, 1246
AlwI GGATC 4 cut(s) 927, 953, 1292, 1305
AoxI GGCC 2 cut(s) 40, 683
ApeKI GCWGC 4 cut(s) 137, 450, 974, 1328
AspS9I GGNCC 4 cut(s) 612, 638, 684, 806
AsuHPI GGTGA 6 cut(s) 415, 605, 890, 1084, 1094, 1309
AvaII GGWCC 3 cut(s) 612, 638, 806
BaeI ACNNNNGTAYC 2 cut(s) 622, 655
BalI TGGCCA 1 cut(s) 42
BamHI GGATCC 1 cut(s) 1297
BarI GAAGNNNNNNTAC 4 cut(s) 482, 514, 530, 562
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 4 cut(s) 124, 437, 961, 1315
BccI CCATC 3 cut(s) 383, 457, 584
BciT130I CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
BcoDI GTCTC 6 cut(s) 236, 526, 710, 761, 1196, 1246
BfmI CTRYAG 1 cut(s) 713
BfrI CTTAAG 1 cut(s) 188
BglI GCCNNNNNGGC 1 cut(s) 682
BisI GCNGC 4 cut(s) 138, 451, 975, 1329
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 4 cut(s) 139, 452, 976, 1330
BmcAI AGTACT 1 cut(s) 485
Bme1390I CCNGG 6 cut(s) 172, 279, 786, 876, 992, 1014
Bme18I GGWCC 3 cut(s) 612, 638, 806
BmeRI GACNNNNNGTC 1 cut(s) 1108
BmgT120I GGNCC 4 cut(s) 612, 638, 684, 806
BmiI GGNNCC 3 cut(s) 639, 1134, 1299
BmrFI CCNGG 6 cut(s) 172, 279, 786, 876, 992, 1014
BmrI ACTGGG 1 cut(s) 1034
BmsI GCATC 8 cut(s) 262, 292, 457, 834, 856, 950, 1147, 1164
BmuI ACTGGG 1 cut(s) 1034
BplI GAGNNNNNCTC 2 cut(s) 1193, 1225
BpmI CTGGAG 2 cut(s) 261, 792
Bpu10I CCTNAGC 1 cut(s) 1311
Bpu1102I GCTNAGC 1 cut(s) 195
BpuEI CTTGAG 3 cut(s) 671, 908, 1145
BsaI GGTCTC 2 cut(s) 236, 1196
BsaJI CCNNGG 9 cut(s) 171, 278, 384, 427, 874, 951, 990, 1012, 1224
BsaXI ACNNNNNCTCC 4 cut(s) 1099, 1124, 1129, 1154
Bsc4I CCNNNNNNNGG 6 cut(s) 390, 581, 652, 715, 1225, 1226
Bse1I ACTGG 8 cut(s) 76, 316, 364, 632, 752, 775, 817, 1040
Bse3DI GCAATG 1 cut(s) 1065
BseBI CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
BseDI CCNNGG 9 cut(s) 171, 278, 384, 427, 874, 951, 990, 1012, 1224
BseGI GGATG 7 cut(s) 394, 472, 524, 724, 941, 961, 986
BseLI CCNNNNNNNGG 6 cut(s) 390, 581, 652, 715, 1225, 1226
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 4 cut(s) 132, 186, 702, 1203
BseNI ACTGG 8 cut(s) 76, 316, 364, 632, 752, 775, 817, 1040
BseXI GCAGC 4 cut(s) 124, 437, 961, 1315
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 2 cut(s) 42, 685
BsiHKAI GWGCWC 1 cut(s) 180
BslFI GGGAC 1 cut(s) 1242
BslI CCNNNNNNNGG 6 cut(s) 390, 581, 652, 715, 1225, 1226
BsmAI GTCTC 6 cut(s) 236, 526, 710, 761, 1196, 1246
BsmBI CGTCTC 1 cut(s) 710
BsmFI GGGAC 1 cut(s) 1242
BsnI GGCC 2 cut(s) 42, 685
Bso31I GGTCTC 2 cut(s) 236, 1196
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 4 cut(s) 666, 919, 958, 1297
Bsp1720I GCTNAGC 1 cut(s) 195
BspANI GGCC 2 cut(s) 42, 685
BspCNI CTCAG 4 cut(s) 133, 187, 701, 1204
BspHI TCATGA 2 cut(s) 850, 1002
BspLI GGNNCC 3 cut(s) 639, 1134, 1299
BspMAI CTGCAG 1 cut(s) 717
BspPI GGATC 4 cut(s) 927, 953, 1292, 1305
BspQI GCTCTTC 2 cut(s) 417, 864
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 2 cut(s) 236, 1196
BsrDI GCAATG 1 cut(s) 1065
BsrI ACTGG 8 cut(s) 76, 316, 364, 632, 752, 775, 817, 1040
BssECI CCNNGG 9 cut(s) 171, 278, 384, 427, 874, 951, 990, 1012, 1224
BssMI GATC 4 cut(s) 666, 919, 958, 1297
BssT1I CCWWGG 4 cut(s) 384, 427, 951, 1224
Bst2UI CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
Bst4CI ACNGT 4 cut(s) 265, 332, 748, 1261
Bst6I CTCTTC 2 cut(s) 417, 864
BstAFI CTTAAG 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 6 cut(s) 141, 195, 688, 1137, 1212, 1311
BstEII GGTNACC 1 cut(s) 1315
BstENI CCTNNNNNAGG 1 cut(s) 650
BstF5I GGATG 7 cut(s) 394, 472, 524, 724, 941, 961, 986
BstKTI GATC 4 cut(s) 669, 922, 961, 1300
BstMAI GTCTC 6 cut(s) 236, 526, 710, 761, 1196, 1246
BstMBI GATC 4 cut(s) 666, 919, 958, 1297
BstMWI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
BstNI CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
BstNSI RCATGY 1 cut(s) 410
BstPI GGTNACC 1 cut(s) 1315
BstSCI CCNGG 6 cut(s) 170, 277, 784, 874, 990, 1012
BstSFI CTRYAG 1 cut(s) 713
BstV1I GCAGC 4 cut(s) 124, 437, 961, 1315
BstX2I RGATCY 2 cut(s) 919, 1297
BstXI CCANNNNNNTGG 1 cut(s) 1192
BstYI RGATCY 2 cut(s) 919, 1297
BsuRI GGCC 2 cut(s) 42, 685
BtsCI GGATG 7 cut(s) 394, 472, 524, 724, 941, 961, 986
BtsI GCAGTG 1 cut(s) 710
BtsIMutI CAGTG 6 cut(s) 69, 261, 357, 710, 759, 822
Cac8I GCNNGC 1 cut(s) 408
CciI TCATGA 2 cut(s) 850, 1002
Cfr13I GGNCC 4 cut(s) 612, 638, 684, 806
Csp6I GTAC 6 cut(s) 166, 253, 484, 499, 631, 1216
CspCI CAANNNNNGTGG 4 cut(s) 604, 639, 715, 750
CviAII CATG 7 cut(s) 44, 304, 407, 601, 851, 1003, 1249
CviQI GTAC 6 cut(s) 166, 253, 484, 499, 631, 1216
DdeI CTNAG 6 cut(s) 141, 195, 688, 1137, 1212, 1311
DpnI GATC 4 cut(s) 668, 921, 960, 1299
DpnII GATC 4 cut(s) 666, 919, 958, 1297
DraI TTTAAA 1 cut(s) 1146
DriI GACNNNNNGTC 1 cut(s) 1108
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 417, 864
Eam1105I GACNNNNNGTC 1 cut(s) 1108
EarI CTCTTC 2 cut(s) 417, 864
Eco130I CCWWGG 4 cut(s) 384, 427, 951, 1224
Eco31I GGTCTC 2 cut(s) 236, 1196
Eco47I GGWCC 3 cut(s) 612, 638, 806
Eco57I CTGAAG 2 cut(s) 845, 846
Eco91I GGTNACC 1 cut(s) 1315
EcoNI CCTNNNNNAGG 1 cut(s) 650
EcoO109I RGGNCCY 2 cut(s) 638, 684
EcoO65I GGTNACC 1 cut(s) 1315
EcoRII CCWGG 6 cut(s) 170, 277, 784, 874, 990, 1012
EcoT14I CCWWGG 4 cut(s) 384, 427, 951, 1224
EcoT22I ATGCAT 1 cut(s) 849
ErhI CCWWGG 4 cut(s) 384, 427, 951, 1224
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 7 cut(s) 47, 307, 410, 604, 854, 1006, 1252
FaqI GGGAC 1 cut(s) 1242
FatI CATG 7 cut(s) 43, 303, 406, 600, 850, 1002, 1248
FblI GTMKAC 2 cut(s) 48, 723
Fnu4HI GCNGC 4 cut(s) 138, 451, 975, 1329
FokI GGATG 7 cut(s) 401, 479, 511, 731, 928, 968, 973
Fsp4HI GCNGC 4 cut(s) 138, 451, 975, 1329
GluI GCNGC 4 cut(s) 138, 451, 975, 1329
GsuI CTGGAG 2 cut(s) 261, 792
HaeIII GGCC 2 cut(s) 42, 685
Hin1II CATG 7 cut(s) 47, 307, 410, 604, 854, 1006, 1252
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 89, 185
HinfI GANTC 2 cut(s) 50, 350
HphI GGTGA 6 cut(s) 415, 605, 890, 1084, 1094, 1309
Hpy166II GTNNAC 4 cut(s) 19, 49, 615, 724
Hpy188I TCNGA 3 cut(s) 691, 819, 1213
Hpy188III TCNNGA 8 cut(s) 287, 582, 650, 851, 863, 1003, 1046, 1279
Hpy8I GTNNAC 4 cut(s) 19, 49, 615, 724
HpyCH4III ACNGT 4 cut(s) 265, 332, 748, 1261
HpyCH4IV ACGT 2 cut(s) 1231, 1284
HpyCH4V TGCA 5 cut(s) 261, 715, 847, 941, 965
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
HpyF3I CTNAG 6 cut(s) 141, 195, 688, 1137, 1212, 1311
HpySE526I ACGT 2 cut(s) 1231, 1284
Hsp92II CATG 7 cut(s) 47, 307, 410, 604, 854, 1006, 1252
Kzo9I GATC 4 cut(s) 666, 919, 958, 1297
LguI GCTCTTC 2 cut(s) 417, 864
LmnI GCTCC 2 cut(s) 280, 1132
Lsp1109I GCAGC 4 cut(s) 124, 437, 961, 1315
LweI GCATC 8 cut(s) 262, 292, 457, 834, 856, 950, 1147, 1164
MaeII ACGT 2 cut(s) 1231, 1284
MaeIII GTNAC 8 cut(s) 287, 455, 545, 593, 796, 878, 1244, 1315
MalI GATC 4 cut(s) 668, 921, 960, 1299
MboI GATC 4 cut(s) 666, 919, 958, 1297
MboII GAAGA 4 cut(s) 404, 547, 851, 1303
MflI RGATCY 2 cut(s) 919, 1297
MhlI GDGCHC 1 cut(s) 180
MlsI TGGCCA 1 cut(s) 42
MluCI AATT 2 cut(s) 507, 561
MluNI TGGCCA 1 cut(s) 42
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 2 cut(s) 833, 969
Mox20I TGGCCA 1 cut(s) 42
Mph1103I ATGCAT 1 cut(s) 849
MscI TGGCCA 1 cut(s) 42
MseI TTAA 5 cut(s) 189, 297, 900, 1145, 1170
MslI CAYNNNNRTG 2 cut(s) 1001, 1070
Msp20I TGGCCA 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 6 cut(s) 172, 279, 786, 876, 992, 1014
MvaI CCWGG 6 cut(s) 172, 279, 786, 876, 992, 1014
MwoI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 971
NdeII GATC 4 cut(s) 666, 919, 958, 1297
NlaIII CATG 7 cut(s) 47, 307, 410, 604, 854, 1006, 1252
NlaIV GGNNCC 3 cut(s) 639, 1134, 1299
NmuCI GTSAC 6 cut(s) 287, 593, 796, 878, 1244, 1315
NsiI ATGCAT 1 cut(s) 849
NspI RCATGY 1 cut(s) 410
PaeI GCATGC 1 cut(s) 410
PagI TCATGA 2 cut(s) 850, 1002
PciSI GCTCTTC 2 cut(s) 417, 864
PfeI GAWTC 1 cut(s) 350
PflFI GACNNNGTC 1 cut(s) 884
PkrI GCNGC 4 cut(s) 139, 452, 976, 1330
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PpuMI RGGWCCY 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 6 cut(s) 170, 277, 784, 874, 990, 1012
PspEI GGTNACC 1 cut(s) 1315
PspGI CCWGG 6 cut(s) 170, 277, 784, 874, 990, 1012
PspN4I GGNNCC 3 cut(s) 639, 1134, 1299
PspPI GGNCC 4 cut(s) 612, 638, 684, 806
PspPPI RGGWCCY 1 cut(s) 638
PstI CTGCAG 1 cut(s) 717
PsuI RGATCY 2 cut(s) 919, 1297
PsyI GACNNNGTC 1 cut(s) 884
RsaI GTAC 6 cut(s) 167, 254, 485, 500, 632, 1217
RsaNI GTAC 6 cut(s) 166, 253, 484, 499, 631, 1216
RseI CAYNNNNRTG 2 cut(s) 1001, 1070
SalI GTCGAC 1 cut(s) 47
SapI GCTCTTC 2 cut(s) 417, 864
SaqAI TTAA 5 cut(s) 189, 297, 900, 1145, 1170
SatI GCNGC 4 cut(s) 138, 451, 975, 1329
Sau3AI GATC 4 cut(s) 666, 919, 958, 1297
Sau96I GGNCC 4 cut(s) 612, 638, 684, 806
ScaI AGTACT 1 cut(s) 485
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 6 cut(s) 172, 279, 786, 876, 992, 1014
SduI GDGCHC 1 cut(s) 180
SfaNI GCATC 8 cut(s) 262, 292, 457, 834, 856, 950, 1147, 1164
SfcI CTRYAG 1 cut(s) 713
SinI GGWCC 3 cut(s) 612, 638, 806
SmiMI CAYNNNNRTG 2 cut(s) 1001, 1070
SmlI CTYRAG 4 cut(s) 188, 650, 923, 1124
SmoI CTYRAG 4 cut(s) 188, 650, 923, 1124
SphI GCATGC 1 cut(s) 410
Sse9I AATT 2 cut(s) 507, 561
StyD4I CCNGG 6 cut(s) 170, 277, 784, 874, 990, 1012
StyI CCWWGG 4 cut(s) 384, 427, 951, 1224
TaaI ACNGT 4 cut(s) 265, 332, 748, 1261
TaiI ACGT 2 cut(s) 1234, 1287
TaqI TCGA 4 cut(s) 48, 62, 180, 488
TasI AATT 2 cut(s) 507, 561
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 5 cut(s) 189, 297, 900, 1145, 1170
Tru9I TTAA 5 cut(s) 189, 297, 900, 1145, 1170
TscAI CASTG 6 cut(s) 76, 268, 364, 717, 759, 829
TseFI GTSAC 6 cut(s) 287, 593, 796, 878, 1244, 1315
TseI GCWGC 4 cut(s) 137, 450, 974, 1328
Tsp45I GTSAC 6 cut(s) 287, 593, 796, 878, 1244, 1315
TspDTI ATGAA 3 cut(s) 161, 519, 1019
TspGWI ACGGA 2 cut(s) 692, 1232
TspRI CASTG 6 cut(s) 76, 268, 364, 717, 759, 829
Tth111I GACNNNGTC 1 cut(s) 884
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 3 cut(s) 612, 638, 806
XagI CCTNNNNNAGG 1 cut(s) 650
XceI RCATGY 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 2 cut(s) 50, 1189
XmiI GTMKAC 2 cut(s) 48, 723
ZrmI AGTACT 1 cut(s) 485
Zsp2I ATGCAT 1 cut(s) 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.