RLG00000008124

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000682
Physical Location & Seq
Reverse (-)
25954318 .. 25956086
1769 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008124

Sequence Viewer

Length: 1200 bp
ATGAACAAAAGGTCATTCAAGTACGCCTGGGTGCTCGACAAGCTTAAGGCTGAGCGTGAGCGTGGTATTACCATTGATATTGCTTTGTGGAAGTTTGAGACCACAAGGTACTACTGCACTGTCATTGATGCTCCTGGGCATCGTGACTTTATTAAGAACATGATTACTGGAACTTCACAGGCTGACTGTGCTGTTCTCATTATTGATTCCACCACTGGTGGTTTTGAGGCTGGTATTTCCAAGGACGGGCAGACCCGTGAGCATGCTCTTCTTGCTTTCACCCTAGGTGTGAAGCAAATGATTTGCTGCTGTAACAAGATGGATGCTACTACTCCCAAGTACTCAAAGGCAAGGTACGATGAAATTGTGAAGGAAGTCTCATCCTATCTGAAGAAGGTTGGTTACAACCCCGACAAAATTGCCTTTGTTCCCATCTCTGGATTTGAGGGTGACAACATGATTGAGAGGTCCACTAACCTTGACTGGTACAAGGGTCCTACCCTTCTTGAGGCACTCGACCAGATCAACGAGCCAAAGAGGCCCTCAGACAAGCCCCTCCGTCTCCCACTGCAGGATGTCTACAAGATTGGTGGTATTGGAACTGTGCCAGTTGGACGTGTTGAGACTGGAGTTATTAAGCCTGGTATGGTTGTCACTTTTGGTCCAACTGGTCTGACCACTGAAGTTAAGTCTGTTGAGATGCATCACGAGGCTCTTCAGGAGGCTCTCCCTGGTGACAATGTCGGGTTCAATGTTAAGAATGTTGCTGTGAAGGATCTCAAGCGTGGTTATGTTGCATCCAACTCCAAGGATGATCCCGCAAAAGAGGCTGCCAACTTCACATCCCAGGTCATCATCATGAACCACCCTGGGCAGATTGGTAATGGTTATGCCCCAGTTCTTGATTGCCACACTTCTCACATTGCTGTGAAGTTCGGTGAAATCCTCACCAAGATTGACAGGAGGTCTGGTAAGGAACTTGAGAAGGAGCCCAAGTTTTTGAAGAATGGAGATGCTGGTATGGTTAAGATGCTTCCCACCAAGCCTATGGTTGTTGAGACCTTCTCTGAGTACCCACCCTTAGGACGTTTTGCTGTCCGTGACATGAGACAGACTGTTGCTGTTGGTGTTATCAAGAACGTGGAGAAGAAGGATCCAAGTGGTGCTAAGGTCACCAAGTCTGCTGCCAAGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

400

Amino Acids

44.05

Weight (kDa)

9.14

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 8 - 175 9.1e-43 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 200 - 265 2.2e-17 Elongation factor Tu domain 2
GTP_EFTU_D3 PF03143 274 - 381 8.2e-27 Elongation factor Tu C-terminal domain
GTP-eEF1A_C PF22594 279 - 379 7.6e-37 GTP-eEF1A C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 579
AciI CCGC 1 cut(s) 819
AclWI GGATC 4 cut(s) 783, 809, 1148, 1161
AcuI CTGAAG 3 cut(s) 410, 701, 702
AfaI GTAC 6 cut(s) 23, 110, 341, 356, 488, 1073
AfiI CCNNNNNNNGG 5 cut(s) 246, 437, 508, 571, 1082
AflII CTTAAG 1 cut(s) 44
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 3 cut(s) 19, 751, 1003
AhdI GACNNNNNGTC 1 cut(s) 964
AjiI CACGTC 1 cut(s) 617
AjnI CCWGG 6 cut(s) 26, 133, 640, 730, 846, 868
AluBI AGCT 1 cut(s) 43
AluI AGCT 1 cut(s) 43
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 6 cut(s) 92, 382, 566, 617, 1052, 1102
AlwI GGATC 4 cut(s) 783, 809, 1148, 1161
AoxI GGCC 1 cut(s) 539
ApeKI GCWGC 3 cut(s) 306, 830, 1184
AspA2I CCTAGG 1 cut(s) 283
AspS9I GGNCC 4 cut(s) 468, 494, 540, 662
AsuHPI GGTGA 6 cut(s) 271, 461, 746, 940, 950, 1165
AvaII GGWCC 3 cut(s) 468, 494, 662
AvrII CCTAGG 1 cut(s) 283
AxyI CCTNAGG 1 cut(s) 1081
BaeI ACNNNNGTAYC 2 cut(s) 478, 511
BamHI GGATCC 1 cut(s) 1153
BanII GRGCYC 1 cut(s) 993
BarI GAAGNNNNNNTAC 2 cut(s) 386, 418
BauI CACGAG 1 cut(s) 707
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 3 cut(s) 293, 817, 1171
BccI CCATC 2 cut(s) 313, 440
BcgI CGANNNNNNTGC 2 cut(s) 401, 435
BciT130I CCWGG 6 cut(s) 28, 135, 642, 732, 848, 870
BcoDI GTCTC 6 cut(s) 92, 382, 566, 617, 1052, 1102
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 1 cut(s) 569
BfrI CTTAAG 1 cut(s) 44
BglI GCCNNNNNGGC 1 cut(s) 538
BisI GCNGC 3 cut(s) 307, 831, 1185
BlnI CCTAGG 1 cut(s) 283
BlpI GCTNAGC 1 cut(s) 51
BlsI GCNGC 3 cut(s) 308, 832, 1186
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 6 cut(s) 28, 135, 642, 732, 848, 870
Bme18I GGWCC 3 cut(s) 468, 494, 662
BmeRI GACNNNNNGTC 1 cut(s) 964
BmgBI CACGTC 1 cut(s) 617
BmgT120I GGNCC 4 cut(s) 468, 494, 540, 662
BmiI GGNNCC 3 cut(s) 495, 990, 1155
BmrFI CCNGG 6 cut(s) 28, 135, 642, 732, 848, 870
BmrI ACTGGG 1 cut(s) 890
BmsI GCATC 8 cut(s) 118, 148, 313, 690, 712, 806, 1003, 1020
BmuI ACTGGG 1 cut(s) 890
BplI GAGNNNNNCTC 2 cut(s) 1049, 1081
BpmI CTGGAG 1 cut(s) 648
Bpu10I CCTNAGC 1 cut(s) 1167
Bpu1102I GCTNAGC 1 cut(s) 51
BpuEI CTTGAG 3 cut(s) 527, 764, 1001
BsaI GGTCTC 2 cut(s) 92, 1052
BsaJI CCNNGG 9 cut(s) 27, 134, 240, 283, 730, 807, 846, 868, 869
BsaXI ACNNNNNCTCC 4 cut(s) 955, 980, 985, 1010
Bsc4I CCNNNNNNNGG 5 cut(s) 246, 437, 508, 571, 1082
Bse1I ACTGG 7 cut(s) 172, 220, 488, 608, 631, 673, 896
Bse21I CCTNAGG 1 cut(s) 1081
Bse3DI GCAATG 1 cut(s) 921
BseBI CCWGG 6 cut(s) 28, 135, 642, 732, 848, 870
BseDI CCNNGG 9 cut(s) 27, 134, 240, 283, 730, 807, 846, 868, 869
BseGI GGATG 6 cut(s) 328, 380, 580, 797, 817, 842
BseLI CCNNNNNNNGG 5 cut(s) 246, 437, 508, 571, 1082
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 3 cut(s) 42, 558, 1059
BseNI ACTGG 7 cut(s) 172, 220, 488, 608, 631, 673, 896
BseXI GCAGC 3 cut(s) 293, 817, 1171
BsgI GTGCAG 1 cut(s) 100
BshFI GGCC 1 cut(s) 541
BsiHKAI GWGCWC 1 cut(s) 36
BslI CCNNNNNNNGG 5 cut(s) 246, 437, 508, 571, 1082
BsmAI GTCTC 6 cut(s) 92, 382, 566, 617, 1052, 1102
BsmBI CGTCTC 1 cut(s) 566
BsnI GGCC 1 cut(s) 541
Bso31I GGTCTC 2 cut(s) 92, 1052
Bsp1286I GDGCHC 2 cut(s) 36, 993
Bsp143I GATC 4 cut(s) 522, 775, 814, 1153
Bsp1720I GCTNAGC 1 cut(s) 51
BspACI CCGC 1 cut(s) 819
BspANI GGCC 1 cut(s) 541
BspCNI CTCAG 3 cut(s) 43, 557, 1060
BspHI TCATGA 1 cut(s) 858
BspLI GGNNCC 3 cut(s) 495, 990, 1155
BspMAI CTGCAG 1 cut(s) 573
BspPI GGATC 4 cut(s) 783, 809, 1148, 1161
BspQI GCTCTTC 2 cut(s) 273, 720
BspTI CTTAAG 1 cut(s) 44
BspTNI GGTCTC 2 cut(s) 92, 1052
BsrDI GCAATG 1 cut(s) 921
BsrI ACTGG 7 cut(s) 172, 220, 488, 608, 631, 673, 896
BssECI CCNNGG 9 cut(s) 27, 134, 240, 283, 730, 807, 846, 868, 869
BssMI GATC 4 cut(s) 522, 775, 814, 1153
BssSI CACGAG 1 cut(s) 707
BssT1I CCWWGG 3 cut(s) 240, 283, 807
Bst2BI CACGAG 1 cut(s) 707
Bst2UI CCWGG 6 cut(s) 28, 135, 642, 732, 848, 870
Bst4CI ACNGT 4 cut(s) 121, 188, 604, 1117
Bst6I CTCTTC 2 cut(s) 273, 720
BstAFI CTTAAG 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 5 cut(s) 51, 544, 1068, 1081, 1167
BstEII GGTNACC 1 cut(s) 1171
BstENI CCTNNNNNAGG 1 cut(s) 506
BstF5I GGATG 6 cut(s) 328, 380, 580, 797, 817, 842
BstKTI GATC 4 cut(s) 525, 778, 817, 1156
BstMAI GTCTC 6 cut(s) 92, 382, 566, 617, 1052, 1102
BstMBI GATC 4 cut(s) 522, 775, 814, 1153
BstMWI GCNNNNNNNGC 5 cut(s) 40, 188, 272, 538, 827
BstNI CCWGG 6 cut(s) 28, 135, 642, 732, 848, 870
BstNSI RCATGY 1 cut(s) 266
BstPI GGTNACC 1 cut(s) 1171
BstSCI CCNGG 6 cut(s) 26, 133, 640, 730, 846, 868
BstSFI CTRYAG 1 cut(s) 569
BstV1I GCAGC 3 cut(s) 293, 817, 1171
BstX2I RGATCY 2 cut(s) 775, 1153
BstXI CCANNNNNNTGG 1 cut(s) 1048
BstYI RGATCY 2 cut(s) 775, 1153
Bsu36I CCTNAGG 1 cut(s) 1081
BsuRI GGCC 1 cut(s) 541
BtrI CACGTC 1 cut(s) 617
BtsCI GGATG 6 cut(s) 328, 380, 580, 797, 817, 842
BtsI GCAGTG 1 cut(s) 566
BtsIMutI CAGTG 4 cut(s) 117, 213, 566, 678
Cac8I GCNNGC 1 cut(s) 264
CciI TCATGA 1 cut(s) 858
Cfr13I GGNCC 4 cut(s) 468, 494, 540, 662
Csp6I GTAC 6 cut(s) 22, 109, 340, 355, 487, 1072
CspCI CAANNNNNGTGG 4 cut(s) 460, 495, 571, 606
CviAII CATG 5 cut(s) 160, 263, 457, 859, 1105
CviQI GTAC 6 cut(s) 22, 109, 340, 355, 487, 1072
DdeI CTNAG 5 cut(s) 51, 544, 1068, 1081, 1167
DpnI GATC 4 cut(s) 524, 777, 816, 1155
DpnII GATC 4 cut(s) 522, 775, 814, 1153
DriI GACNNNNNGTC 1 cut(s) 964
Eam1104I CTCTTC 2 cut(s) 273, 720
Eam1105I GACNNNNNGTC 1 cut(s) 964
EarI CTCTTC 2 cut(s) 273, 720
Eco130I CCWWGG 3 cut(s) 240, 283, 807
Eco24I GRGCYC 1 cut(s) 993
Eco31I GGTCTC 2 cut(s) 92, 1052
Eco47I GGWCC 3 cut(s) 468, 494, 662
Eco57I CTGAAG 3 cut(s) 410, 701, 702
Eco81I CCTNAGG 1 cut(s) 1081
Eco91I GGTNACC 1 cut(s) 1171
EcoNI CCTNNNNNAGG 1 cut(s) 506
EcoO109I RGGNCCY 2 cut(s) 494, 540
EcoO65I GGTNACC 1 cut(s) 1171
EcoRII CCWGG 6 cut(s) 26, 133, 640, 730, 846, 868
EcoT14I CCWWGG 3 cut(s) 240, 283, 807
EcoT22I ATGCAT 1 cut(s) 705
EcoT38I GRGCYC 1 cut(s) 993
ErhI CCWWGG 3 cut(s) 240, 283, 807
Esp3I CGTCTC 1 cut(s) 566
FaeI CATG 5 cut(s) 163, 266, 460, 862, 1108
FatI CATG 5 cut(s) 159, 262, 456, 858, 1104
FauI CCCGC 1 cut(s) 826
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 3 cut(s) 307, 831, 1185
FokI GGATG 6 cut(s) 335, 367, 587, 784, 824, 829
FriOI GRGCYC 1 cut(s) 993
Fsp4HI GCNGC 3 cut(s) 307, 831, 1185
FspBI CTAG 1 cut(s) 284
GluI GCNGC 3 cut(s) 307, 831, 1185
GsuI CTGGAG 1 cut(s) 648
HaeIII GGCC 1 cut(s) 541
Hin1II CATG 5 cut(s) 163, 266, 460, 862, 1108
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 1 cut(s) 206
HphI GGTGA 6 cut(s) 271, 461, 746, 940, 950, 1165
Hpy166II GTNNAC 2 cut(s) 471, 580
Hpy188I TCNGA 4 cut(s) 390, 547, 675, 1069
Hpy188III TCNNGA 8 cut(s) 143, 438, 506, 707, 719, 859, 902, 1135
Hpy8I GTNNAC 2 cut(s) 471, 580
HpyAV CCTTC 7 cut(s) 364, 388, 512, 766, 979, 1072, 1144
HpyCH4III ACNGT 4 cut(s) 121, 188, 604, 1117
HpyCH4IV ACGT 3 cut(s) 616, 1087, 1140
HpyCH4V TGCA 4 cut(s) 117, 571, 703, 797
HpyF10VI GCNNNNNNNGC 5 cut(s) 40, 188, 272, 538, 827
HpyF3I CTNAG 5 cut(s) 51, 544, 1068, 1081, 1167
HpySE526I ACGT 3 cut(s) 616, 1087, 1140
Hsp92II CATG 5 cut(s) 163, 266, 460, 862, 1108
Kzo9I GATC 4 cut(s) 522, 775, 814, 1153
LguI GCTCTTC 2 cut(s) 273, 720
LmnI GCTCC 2 cut(s) 136, 988
Lsp1109I GCAGC 3 cut(s) 293, 817, 1171
LweI GCATC 8 cut(s) 118, 148, 313, 690, 712, 806, 1003, 1020
MaeI CTAG 1 cut(s) 284
MaeII ACGT 3 cut(s) 616, 1087, 1140
MaeIII GTNAC 8 cut(s) 143, 311, 401, 449, 652, 734, 1100, 1171
MalI GATC 4 cut(s) 524, 777, 816, 1155
MboI GATC 4 cut(s) 522, 775, 814, 1153
MboII GAAGA 5 cut(s) 260, 403, 707, 1015, 1159
MflI RGATCY 2 cut(s) 775, 1153
MhlI GDGCHC 2 cut(s) 36, 993
MluCI AATT 2 cut(s) 363, 417
MmeI TCCRAC 3 cut(s) 592, 689, 825
Mph1103I ATGCAT 1 cut(s) 705
MseI TTAA 6 cut(s) 45, 153, 636, 687, 756, 1026
MslI CAYNNNNRTG 2 cut(s) 857, 926
MspCI CTTAAG 1 cut(s) 44
MspR9I CCNGG 6 cut(s) 28, 135, 642, 732, 848, 870
MvaI CCWGG 6 cut(s) 28, 135, 642, 732, 848, 870
MwoI GCNNNNNNNGC 5 cut(s) 40, 188, 272, 538, 827
NdeII GATC 4 cut(s) 522, 775, 814, 1153
NlaIII CATG 5 cut(s) 163, 266, 460, 862, 1108
NlaIV GGNNCC 3 cut(s) 495, 990, 1155
NmuCI GTSAC 6 cut(s) 143, 449, 652, 734, 1100, 1171
NsiI ATGCAT 1 cut(s) 705
NspI RCATGY 1 cut(s) 266
PaeI GCATGC 1 cut(s) 266
PagI TCATGA 1 cut(s) 858
PasI CCCWGGG 1 cut(s) 869
PciSI GCTCTTC 2 cut(s) 273, 720
PfeI GAWTC 1 cut(s) 206
PflFI GACNNNGTC 1 cut(s) 740
PkrI GCNGC 3 cut(s) 308, 832, 1186
PpuMI RGGWCCY 1 cut(s) 494
Psp5II RGGWCCY 1 cut(s) 494
Psp6I CCWGG 6 cut(s) 26, 133, 640, 730, 846, 868
PspEI GGTNACC 1 cut(s) 1171
PspGI CCWGG 6 cut(s) 26, 133, 640, 730, 846, 868
PspN4I GGNNCC 3 cut(s) 495, 990, 1155
PspPI GGNCC 4 cut(s) 468, 494, 540, 662
PspPPI RGGWCCY 1 cut(s) 494
PstI CTGCAG 1 cut(s) 573
PsuI RGATCY 2 cut(s) 775, 1153
PsyI GACNNNGTC 1 cut(s) 740
RsaI GTAC 6 cut(s) 23, 110, 341, 356, 488, 1073
RsaNI GTAC 6 cut(s) 22, 109, 340, 355, 487, 1072
RseI CAYNNNNRTG 2 cut(s) 857, 926
SapI GCTCTTC 2 cut(s) 273, 720
SaqAI TTAA 6 cut(s) 45, 153, 636, 687, 756, 1026
SatI GCNGC 3 cut(s) 307, 831, 1185
Sau3AI GATC 4 cut(s) 522, 775, 814, 1153
Sau96I GGNCC 4 cut(s) 468, 494, 540, 662
ScaI AGTACT 1 cut(s) 341
ScrFI CCNGG 6 cut(s) 28, 135, 642, 732, 848, 870
SduI GDGCHC 2 cut(s) 36, 993
SfaNI GCATC 8 cut(s) 118, 148, 313, 690, 712, 806, 1003, 1020
SfcI CTRYAG 1 cut(s) 569
SinI GGWCC 3 cut(s) 468, 494, 662
SmiMI CAYNNNNRTG 2 cut(s) 857, 926
SmlI CTYRAG 4 cut(s) 44, 506, 779, 980
SmoI CTYRAG 4 cut(s) 44, 506, 779, 980
SphI GCATGC 1 cut(s) 266
Sse9I AATT 2 cut(s) 363, 417
SsiI CCGC 1 cut(s) 819
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 6 cut(s) 26, 133, 640, 730, 846, 868
StyI CCWWGG 3 cut(s) 240, 283, 807
TaaI ACNGT 4 cut(s) 121, 188, 604, 1117
TaiI ACGT 3 cut(s) 619, 1090, 1143
TaqI TCGA 2 cut(s) 36, 516
TasI AATT 2 cut(s) 363, 417
TatI WGTACW 1 cut(s) 339
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 6 cut(s) 45, 153, 636, 687, 756, 1026
Tru9I TTAA 6 cut(s) 45, 153, 636, 687, 756, 1026
TscAI CASTG 4 cut(s) 124, 220, 573, 685
TseFI GTSAC 6 cut(s) 143, 449, 652, 734, 1100, 1171
TseI GCWGC 3 cut(s) 306, 830, 1184
Tsp45I GTSAC 6 cut(s) 143, 449, 652, 734, 1100, 1171
TspDTI ATGAA 3 cut(s) 17, 375, 875
TspGWI ACGGA 2 cut(s) 548, 1088
TspRI CASTG 4 cut(s) 124, 220, 573, 685
Tth111I GACNNNGTC 1 cut(s) 740
Vha464I CTTAAG 1 cut(s) 44
VpaK11BI GGWCC 3 cut(s) 468, 494, 662
XagI CCTNNNNNAGG 1 cut(s) 506
XceI RCATGY 1 cut(s) 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 1045
XmaJI CCTAGG 1 cut(s) 283
XmiI GTMKAC 1 cut(s) 579
XspI CTAG 1 cut(s) 284
ZrmI AGTACT 1 cut(s) 341
Zsp2I ATGCAT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.