Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000682

Physical Location & Seq

Genomic Coordinates Forward (+)

11881266 .. 11882849

Gene Structure 1584 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1131300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGGGTAAGGAGAAGTTCCACATCAACATCGTGGTCATTGGCCATGTCGACTCTGGGAAGTCGACCACGACAGGTCACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTTATTGAGAGGTTCGAGAAGGAGGCTGCTGAGATGAACAAGAGGTCATTCAAGTATGCCTGGGTGTTGGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGACATTGCCCTGTGGAAGTTCGAGACCACCAAGTACTACTGCACTGTCATTGATGCTCCTGGACATCGTGACTTTATCAAGAACATGATTACTGGAACCTCACAGGCTGACTGTGCCATTCTCATCATTGACTCTACCACCGGAGGTTTTGAAGCCGGTATTTCCAAGGATGGTCAGACCCGTGAGCATGCTTTGCTTGCTTTTACTCTTGGCGTCAGGCAAATGATTTGCTGCTGCAACAAGATGGATGCCACCACTCCCAAGTACTCAAGGGCAAGGTATGATGAAATTGTGAAGGAAGTGTCGTCCTATCTCAAGAAGGTTGGCTACAACCCAGATAAGATCCCCTTTGTCCCCATTTCTGGGTTCGAGGGTGACAACATGATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGTCCCACCCTTCTTGAGGCTCTTGACCAGATCAACGAGCCCAAGAGGCCCTCAGACAAGCCCCTCCGGCTTCCACTTCAGGATGTGTACAAGATTGGTGGTATCGGTACCGTTCCTGTTGGACGTGTTGAGACTGGTGTCATTAAGCCTGGTATGGTTGTGACTTTTGGCCCAACTGGTCTGACTACTGAGGTCAAGTCTGTTGAGATGCACCACGAAGCTATGCAGGAGGCCCTTCCAGGTGATAATGTTGGATTCAACGTTAAGAATGTTGCTGTCAAGGATCTCAAGCGTGGGTACGTTGCTTCCAACTCCAAGGATGATCCCGCAAAGGAGGCTGCCAACTTTATCGCTCAGGTCATCATCATGAACCACCCCGGCCAGATTGGACAGGGATATGCTCCAGTTCTCGACTGTCACACCTCCCACATTGCCGTCAAGTTTGCTGAGCTCGTTACAAAGATCGACAGGCGATCTGGCAAGGAGCTTGAGAAGGAGCCCAAGTTTTTGAAGAATGGTGATGCTGGATTTGTGAAGATGCTTCCCACCAAGCCCATGGTTGTTGAGACCTTCTCTGAGTACCCACCGCTCGGACGTTTTGCTGTGAGGGACATGCGCCAGACTGTTGCAGTTGGTGTCATCAAGAGCGTTGAGAAGAAGGATCCAACTGGAGCTAAGATCACCAAGGCCGCAGCTAAGAAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0003824 GO:0003924 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006412 GO:0006414 GO:0006518 GO:0006807 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0071704 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03013 ko05134 map03013 map05134

Pfam Domains

Protein Families

GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

448

Amino Acids

49.48

Weight (kDa)

9.15

Isoelectric Point (pI)

30.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	7 - 223	3.9e-58	Elongation factor Tu GTP binding domain
GTP_EFTU_D2	PF03144	248 - 313	3.7e-17	Elongation factor Tu domain 2
GTP_EFTU_D3	PF03143	322 - 429	4.5e-27	Elongation factor Tu C-terminal domain
GTP-eEF1A_C	PF22594	327 - 427	4.4e-37	GTP-eEF1A C-terminal domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000682)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07920 AT1G07930 AT1G07930 AT1G07940 AT1G07940 AT1G07940 AT1G07940 AT5G60390 AT5G60390 AT5G60390
fragaria_vesca	FvH4_3g33060 FvH4_3g33150 FvH4_3g33150 FvH4_3g33150 FvH4_7g20050 FvH4_7g20050 FvH4_7g20050
malus_domestica	MD03G1103900.v1.1 MD03G1104900.v1.1 MD04G1011000.v1.1 MD04G1011300.v1.1 MD04G1011900.v1.1 MD11G1130600.v1.1 MD11G1131300.v1.1
prunus_persica	Prupe.1G010400_v2.0.a1 Prupe.2G217300_v2.0.a1 Prupe.6G095200_v2.0.a1 Prupe.6G095700_v2.0.a1 Prupe.6G095700_v2.0.a1
pyrus_communis	pycom04g00890 pycom04g00950 pycom11g10820 pycom11g10850
rosa_chinensis	RchiOBHm_Chr1g0364011 RchiOBHm_Chr4g0414681 RchiOBHm_Chr5g0060421 RchiOBHm_Chr5g0060471
rosa_laevigata	RLG00000008124 RLG00000027528 RLG00000035362 RLG00000035365
rosa_multiflora	Rmu_sc0001192.1_g000021 Rmu_sc0001607.1_g000017 Rmu_sc0003270.1_g000011 Rmu_sc0010606.1_g000001 Rmu_sc0012322.1_g000011 Rmu_ssc0000167.1_g000016
rosa_roxburghii	Rroxscaffold_1G00020320 Rroxscaffold_1G00020410 Rroxscaffold_4G00292750 Rroxscaffold_5G00358500
rosa_rugosa	Rorug01G0312000 Rorug04G0129500 Rorug05G0329800 Rorug05G0330500
rosa_samantha	Rh1AG320000 Rh1BG282900 Rh1CG299600 Rh1DG314500 Rh4AG188000 Rh4BG186700 Rh4CG200100 Rh4DG186900 Rh5AG394500 Rh5AG394900 Rh5BG406700 Rh5BG407300 Rh5CG430500 Rh5CG431000 Rh5DG421800 Rh5DG422100
rosa_wichuraiana	Rw1G028370 Rw4G016060 Rw5G037110 Rw5G037140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	743
AccB1I	GGYRCC	1 cut(s)	743
AccBSI	CCGCTC	1 cut(s)	1225
AccI	GTMKAC	2 cut(s)	48, 62
AciI	CCGC	3 cut(s)	963, 1223, 1326
AclI	AACGTT	1 cut(s)	897
AclWI	GGATC	5 cut(s)	556, 927, 953, 1292, 1305
AcoI	YGGCCR	2 cut(s)	40, 1015
AcuI	CTGAAG	1 cut(s)	698
AcyI	GRCGYC	1 cut(s)	432
AfaI	GTAC	7 cut(s)	254, 485, 632, 725, 745, 935, 1217
AfiI	CCNNNNNNNGG	4 cut(s)	581, 582, 652, 1226
AflIII	ACRYGT	1 cut(s)	760
AgsI	TTSAA	4 cut(s)	163, 371, 895, 1147
AjiI	CACGTC	1 cut(s)	761
AjnI	CCWGG	4 cut(s)	170, 277, 784, 874
AluBI	AGCT	7 cut(s)	91, 187, 857, 1087, 1123, 1310, 1331
AluI	AGCT	7 cut(s)	91, 187, 857, 1087, 1123, 1310, 1331
Alw21I	GWGCWC	1 cut(s)	1089
Alw26I	GTCTC	3 cut(s)	236, 761, 1196
AlwI	GGATC	5 cut(s)	556, 927, 953, 1292, 1305
AoxI	GGCC	6 cut(s)	40, 683, 805, 867, 1015, 1323
ApeKI	GCWGC	5 cut(s)	137, 450, 453, 974, 1328
Asp718I	GGTACC	1 cut(s)	743
AspLEI	GCGC	1 cut(s)	1254
AspS9I	GGNCC	5 cut(s)	612, 638, 684, 806, 868
AsuC2I	CCSGG	1 cut(s)	1014
AsuHPI	GGTGA	4 cut(s)	605, 890, 1166, 1309
AvaII	GGWCC	2 cut(s)	612, 638
BaeI	ACNNNNGTAYC	2 cut(s)	622, 655
BalI	TGGCCA	1 cut(s)	42
BamHI	GGATCC	1 cut(s)	1297
BanI	GGYRCC	1 cut(s)	743
BanII	GRGCYC	3 cut(s)	678, 1089, 1137
BarI	GAAGNNNNNNTAC	2 cut(s)	530, 562
Bbv12I	GWGCWC	1 cut(s)	1089
BbvI	GCAGC	5 cut(s)	124, 437, 440, 961, 1340
BccI	CCATC	2 cut(s)	383, 457
BceAI	ACGGC	1 cut(s)	1055
BciT130I	CCWGG	4 cut(s)	172, 279, 786, 876
BcnI	CCSGG	1 cut(s)	1014
BcoDI	GTCTC	3 cut(s)	236, 761, 1196
BglI	GCCNNNNNGGC	2 cut(s)	682, 703
BisI	GCNGC	6 cut(s)	138, 451, 454, 975, 1326, 1329
BlpI	GCTNAGC	2 cut(s)	195, 1083
BlsI	GCNGC	6 cut(s)	139, 452, 455, 976, 1327, 1330
BmcAI	AGTACT	2 cut(s)	254, 485
Bme1390I	CCNGG	5 cut(s)	172, 279, 786, 876, 1014
Bme18I	GGWCC	2 cut(s)	612, 638
BmgBI	CACGTC	1 cut(s)	761
BmgT120I	GGNCC	5 cut(s)	612, 638, 684, 806, 868
BmiI	GGNNCC	6 cut(s)	316, 639, 640, 745, 1134, 1299
BmrFI	CCNGG	5 cut(s)	172, 279, 786, 876, 1014
BmsI	GCATC	5 cut(s)	262, 457, 834, 1147, 1164
BoxI	GACNNNNGTC	1 cut(s)	773
BplI	GAGNNNNNCTC	2 cut(s)	1193, 1225
BpmI	CTGGAG	2 cut(s)	1023, 1326
Bpu10I	CCTNAGC	1 cut(s)	990
Bpu1102I	GCTNAGC	2 cut(s)	195, 1083
BpuEI	CTTGAG	6 cut(s)	173, 472, 518, 671, 908, 1145
BpuMI	CCSGG	1 cut(s)	1014
BsaHI	GRCGYC	1 cut(s)	432
BsaI	GGTCTC	2 cut(s)	236, 1196
BsaJI	CCNNGG	6 cut(s)	171, 384, 951, 1012, 1191, 1320
BsaWI	WCCGGW	1 cut(s)	359
BsaXI	ACNNNNNCTCC	2 cut(s)	1124, 1154
Bsc4I	CCNNNNNNNGG	4 cut(s)	581, 582, 652, 1226
Bse118I	RCCGGY	1 cut(s)	374
Bse1I	ACTGG	6 cut(s)	316, 632, 775, 817, 1040, 1309
Bse3DI	GCAATG	2 cut(s)	222, 1065
BseBI	CCWGG	4 cut(s)	172, 279, 786, 876
BseDI	CCNNGG	6 cut(s)	171, 384, 951, 1012, 1191, 1320
BseGI	GGATG	4 cut(s)	394, 472, 724, 961
BseLI	CCNNNNNNNGG	4 cut(s)	581, 582, 652, 1226
BseMI	GCAATG	2 cut(s)	222, 1065
BseMII	CTCAG	7 cut(s)	132, 186, 702, 816, 1004, 1074, 1203
BseNI	ACTGG	6 cut(s)	316, 632, 775, 817, 1040, 1309
BseXI	GCAGC	5 cut(s)	124, 437, 440, 961, 1340
BsgI	GTGCAG	1 cut(s)	244
BshFI	GGCC	6 cut(s)	42, 685, 807, 869, 1017, 1325
BshNI	GGYRCC	1 cut(s)	743
BsiHKAI	GWGCWC	1 cut(s)	1089
BsiSI	CCGG	4 cut(s)	360, 375, 703, 1014
BslFI	GGGAC	3 cut(s)	557, 624, 1259
BslI	CCNNNNNNNGG	4 cut(s)	581, 582, 652, 1226
BsmAI	GTCTC	3 cut(s)	236, 761, 1196
BsmFI	GGGAC	3 cut(s)	557, 624, 1259
BsnI	GGCC	6 cut(s)	42, 685, 807, 869, 1017, 1325
Bso31I	GGTCTC	2 cut(s)	236, 1196
Bsp1286I	GDGCHC	3 cut(s)	678, 1089, 1137
Bsp1407I	TGTACA	1 cut(s)	723
Bsp143I	GATC	9 cut(s)	81, 561, 666, 919, 958, 1098, 1109, 1297, 1314
Bsp1720I	GCTNAGC	2 cut(s)	195, 1083
Bsp19I	CCATGG	1 cut(s)	1191
BspACI	CCGC	3 cut(s)	963, 1223, 1326
BspANI	GGCC	6 cut(s)	42, 685, 807, 869, 1017, 1325
BspCNI	CTCAG	7 cut(s)	133, 187, 701, 817, 1003, 1075, 1204
BspHI	TCATGA	1 cut(s)	1002
BspLI	GGNNCC	6 cut(s)	316, 639, 640, 745, 1134, 1299
BspPI	GGATC	5 cut(s)	556, 927, 953, 1292, 1305
BspT107I	GGYRCC	1 cut(s)	743
BspTNI	GGTCTC	2 cut(s)	236, 1196
BsrBI	CCGCTC	1 cut(s)	1225
BsrDI	GCAATG	2 cut(s)	222, 1065
BsrFI	RCCGGY	1 cut(s)	374
BsrGI	TGTACA	1 cut(s)	723
BsrI	ACTGG	6 cut(s)	316, 632, 775, 817, 1040, 1309
BssAI	RCCGGY	1 cut(s)	374
BssECI	CCNNGG	6 cut(s)	171, 384, 951, 1012, 1191, 1320
BssMI	GATC	9 cut(s)	81, 561, 666, 919, 958, 1098, 1109, 1297, 1314
BssNI	GRCGYC	1 cut(s)	432
BssT1I	CCWWGG	4 cut(s)	384, 951, 1191, 1320
Bst2UI	CCWGG	4 cut(s)	172, 279, 786, 876
Bst4CI	ACNGT	5 cut(s)	265, 332, 748, 1052, 1261
BstACI	GRCGYC	1 cut(s)	432
BstAPI	GCANNNNNTGC	1 cut(s)	412
BstAUI	TGTACA	1 cut(s)	723
BstC8I	GCNNGC	2 cut(s)	408, 417
BstDEI	CTNAG	9 cut(s)	141, 195, 688, 825, 990, 1083, 1212, 1311, 1332
BstDSI	CCRYGG	1 cut(s)	1191
BstENI	CCTNNNNNAGG	1 cut(s)	650
BstF5I	GGATG	4 cut(s)	394, 472, 724, 961
BstHHI	GCGC	1 cut(s)	1254
BstKTI	GATC	9 cut(s)	84, 564, 669, 922, 961, 1101, 1112, 1300, 1317
BstMAI	GTCTC	3 cut(s)	236, 761, 1196
BstMBI	GATC	9 cut(s)	81, 561, 666, 919, 958, 1098, 1109, 1297, 1314
BstMWI	GCNNNNNNNGC	6 cut(s)	332, 412, 416, 682, 703, 971
BstNI	CCWGG	4 cut(s)	172, 279, 786, 876
BstNSI	RCATGY	2 cut(s)	410, 1252
BstPAI	GACNNNNGTC	1 cut(s)	773
BstSCI	CCNGG	5 cut(s)	170, 277, 784, 874, 1012
BstV1I	GCAGC	5 cut(s)	124, 437, 440, 961, 1340
BstX2I	RGATCY	3 cut(s)	561, 919, 1297
BstXI	CCANNNNNNTGG	1 cut(s)	1192
BstYI	RGATCY	3 cut(s)	561, 919, 1297
BsuRI	GGCC	6 cut(s)	42, 685, 807, 869, 1017, 1325
BtgI	CCRYGG	1 cut(s)	1191
BtrI	CACGTC	1 cut(s)	761
BtsCI	GGATG	4 cut(s)	394, 472, 724, 961
BtsIMutI	CAGTG	1 cut(s)	261
Cac8I	GCNNGC	2 cut(s)	408, 417
CciI	TCATGA	1 cut(s)	1002
CfoI	GCGC	1 cut(s)	1254
Cfr10I	RCCGGY	1 cut(s)	374
Cfr13I	GGNCC	5 cut(s)	612, 638, 684, 806, 868
CseI	GACGC	1 cut(s)	421
Csp6I	GTAC	7 cut(s)	253, 484, 631, 724, 744, 934, 1216
CspCI	CAANNNNNGTGG	8 cut(s)	76, 111, 604, 631, 639, 666, 715, 750
CviAII	CATG	7 cut(s)	44, 304, 407, 601, 1003, 1192, 1249
CviQI	GTAC	7 cut(s)	253, 484, 631, 724, 744, 934, 1216
DdeI	CTNAG	9 cut(s)	141, 195, 688, 825, 990, 1083, 1212, 1311, 1332
DpnI	GATC	9 cut(s)	83, 563, 668, 921, 960, 1100, 1111, 1299, 1316
DpnII	GATC	9 cut(s)	81, 561, 666, 919, 958, 1098, 1109, 1297, 1314
EaeI	YGGCCR	2 cut(s)	40, 1015
Ecl136II	GAGCTC	1 cut(s)	1087
Eco130I	CCWWGG	4 cut(s)	384, 951, 1191, 1320
Eco24I	GRGCYC	3 cut(s)	678, 1089, 1137
Eco31I	GGTCTC	2 cut(s)	236, 1196
Eco47I	GGWCC	2 cut(s)	612, 638
Eco53kI	GAGCTC	1 cut(s)	1087
Eco57I	CTGAAG	1 cut(s)	698
EcoICRI	GAGCTC	1 cut(s)	1087
EcoNI	CCTNNNNNAGG	1 cut(s)	650
EcoO109I	RGGNCCY	3 cut(s)	638, 684, 868
EcoRII	CCWGG	4 cut(s)	170, 277, 784, 874
EcoT14I	CCWWGG	4 cut(s)	384, 951, 1191, 1320
EcoT38I	GRGCYC	3 cut(s)	678, 1089, 1137
ErhI	CCWWGG	4 cut(s)	384, 951, 1191, 1320
FaeI	CATG	7 cut(s)	47, 307, 410, 604, 1006, 1195, 1252
FalI	AAGNNNNNCTT	2 cut(s)	551, 583
FaqI	GGGAC	3 cut(s)	557, 624, 1259
FatI	CATG	7 cut(s)	43, 303, 406, 600, 1002, 1191, 1248
FauI	CCCGC	1 cut(s)	970
FblI	GTMKAC	2 cut(s)	48, 62
Fnu4HI	GCNGC	6 cut(s)	138, 451, 454, 975, 1326, 1329
FokI	GGATG	4 cut(s)	401, 479, 731, 968
FriOI	GRGCYC	3 cut(s)	678, 1089, 1137
Fsp4HI	GCNGC	6 cut(s)	138, 451, 454, 975, 1326, 1329
GlaI	GCGC	1 cut(s)	1253
GluI	GCNGC	6 cut(s)	138, 451, 454, 975, 1326, 1329
GsuI	CTGGAG	2 cut(s)	1023, 1326
HaeIII	GGCC	6 cut(s)	42, 685, 807, 869, 1017, 1325
HapII	CCGG	4 cut(s)	360, 375, 703, 1014
HgaI	GACGC	1 cut(s)	421
HhaI	GCGC	1 cut(s)	1254
Hin1I	GRCGYC	1 cut(s)	432
Hin1II	CATG	7 cut(s)	47, 307, 410, 604, 1006, 1195, 1252
Hin6I	GCGC	1 cut(s)	1252
HinP1I	GCGC	1 cut(s)	1252
HincII	GTYRAC	2 cut(s)	49, 63
HindII	GTYRAC	2 cut(s)	49, 63
HindIII	AAGCTT	1 cut(s)	89
HinfI	GANTC	3 cut(s)	50, 350, 891
HpaII	CCGG	4 cut(s)	360, 375, 703, 1014
HphI	GGTGA	4 cut(s)	605, 890, 1166, 1309
Hpy166II	GTNNAC	5 cut(s)	49, 63, 203, 615, 724
Hpy188I	TCNGA	5 cut(s)	396, 691, 819, 1213, 1229
Hpy8I	GTNNAC	5 cut(s)	49, 63, 203, 615, 724
HpyAV	CCTTC	8 cut(s)	124, 508, 532, 656, 881, 1123, 1216, 1288
HpyCH4III	ACNGT	5 cut(s)	265, 332, 748, 1052, 1261
HpyCH4IV	ACGT	5 cut(s)	205, 760, 897, 936, 1231
HpyCH4V	TGCA	5 cut(s)	261, 456, 847, 862, 1265
HpyF10VI	GCNNNNNNNGC	6 cut(s)	332, 412, 416, 682, 703, 971
HpyF3I	CTNAG	9 cut(s)	141, 195, 688, 825, 990, 1083, 1212, 1311, 1332
HpySE526I	ACGT	5 cut(s)	205, 760, 897, 936, 1231
Hsp92I	GRCGYC	1 cut(s)	432
Hsp92II	CATG	7 cut(s)	47, 307, 410, 604, 1006, 1195, 1252
HspAI	GCGC	1 cut(s)	1252
KflI	GGGWCCC	1 cut(s)	638
KpnI	GGTACC	1 cut(s)	747
Kzo9I	GATC	9 cut(s)	81, 561, 666, 919, 958, 1098, 1109, 1297, 1314
LmnI	GCTCC	5 cut(s)	280, 1042, 1120, 1132, 1307
Lsp1109I	GCAGC	5 cut(s)	124, 437, 440, 961, 1340
LweI	GCATC	5 cut(s)	262, 457, 834, 1147, 1164
MaeII	ACGT	5 cut(s)	205, 760, 897, 936, 1231
MaeIII	GTNAC	6 cut(s)	74, 287, 593, 796, 1052, 1090
MalI	GATC	9 cut(s)	83, 563, 668, 921, 960, 1100, 1111, 1299, 1316
MbiI	CCGCTC	1 cut(s)	1225
MboI	GATC	9 cut(s)	81, 561, 666, 919, 958, 1098, 1109, 1297, 1314
MboII	GAAGA	3 cut(s)	1159, 1183, 1303
MflI	RGATCY	3 cut(s)	561, 919, 1297
MhlI	GDGCHC	3 cut(s)	678, 1089, 1137
MlsI	TGGCCA	1 cut(s)	42
MluCI	AATT	1 cut(s)	507
MluNI	TGGCCA	1 cut(s)	42
MlyI	GAGTC	2 cut(s)	44, 344
MmeI	TCCRAC	5 cut(s)	159, 736, 868, 969, 1325
Mox20I	TGGCCA	1 cut(s)	42
MscI	TGGCCA	1 cut(s)	42
MseI	TTAA	2 cut(s)	780, 900
MslI	CAYNNNNRTG	1 cut(s)	1001
Msp20I	TGGCCA	1 cut(s)	42
MspI	CCGG	4 cut(s)	360, 375, 703, 1014
MspR9I	CCNGG	5 cut(s)	172, 279, 786, 876, 1014
MvaI	CCWGG	4 cut(s)	172, 279, 786, 876
MwoI	GCNNNNNNNGC	6 cut(s)	332, 412, 416, 682, 703, 971
NciI	CCSGG	1 cut(s)	1014
NcoI	CCATGG	1 cut(s)	1191
NdeII	GATC	9 cut(s)	81, 561, 666, 919, 958, 1098, 1109, 1297, 1314
NlaIII	CATG	7 cut(s)	47, 307, 410, 604, 1006, 1195, 1252
NlaIV	GGNNCC	6 cut(s)	316, 639, 640, 745, 1134, 1299
NmuCI	GTSAC	5 cut(s)	74, 287, 593, 796, 1052
NspI	RCATGY	2 cut(s)	410, 1252
PaeI	GCATGC	1 cut(s)	410
PagI	TCATGA	1 cut(s)	1002
PfeI	GAWTC	1 cut(s)	891
PfoI	TCCNGGA	1 cut(s)	277
PkrI	GCNGC	6 cut(s)	139, 452, 455, 976, 1327, 1330
PleI	GAGTC	2 cut(s)	44, 344
PpsI	GAGTC	2 cut(s)	44, 344
PpuMI	RGGWCCY	1 cut(s)	638
PshAI	GACNNNNGTC	1 cut(s)	773
Psp124BI	GAGCTC	1 cut(s)	1089
Psp1406I	AACGTT	1 cut(s)	897
Psp5II	RGGWCCY	1 cut(s)	638
Psp6I	CCWGG	4 cut(s)	170, 277, 784, 874
PspGI	CCWGG	4 cut(s)	170, 277, 784, 874
PspN4I	GGNNCC	6 cut(s)	316, 639, 640, 745, 1134, 1299
PspPI	GGNCC	5 cut(s)	612, 638, 684, 806, 868
PspPPI	RGGWCCY	1 cut(s)	638
PsuI	RGATCY	3 cut(s)	561, 919, 1297
RsaI	GTAC	7 cut(s)	254, 485, 632, 725, 745, 935, 1217
RsaNI	GTAC	7 cut(s)	253, 484, 631, 724, 744, 934, 1216
RseI	CAYNNNNRTG	1 cut(s)	1001
SacI	GAGCTC	1 cut(s)	1089
SalI	GTCGAC	2 cut(s)	47, 61
SaqAI	TTAA	2 cut(s)	780, 900
SatI	GCNGC	6 cut(s)	138, 451, 454, 975, 1326, 1329
Sau3AI	GATC	9 cut(s)	81, 561, 666, 919, 958, 1098, 1109, 1297, 1314
Sau96I	GGNCC	5 cut(s)	612, 638, 684, 806, 868
ScaI	AGTACT	2 cut(s)	254, 485
SchI	GAGTC	2 cut(s)	44, 344
ScrFI	CCNGG	5 cut(s)	172, 279, 786, 876, 1014
SduI	GDGCHC	3 cut(s)	678, 1089, 1137
SfaNI	GCATC	5 cut(s)	262, 457, 834, 1147, 1164
SinI	GGWCC	2 cut(s)	612, 638
SmiMI	CAYNNNNRTG	1 cut(s)	1001
SmlI	CTYRAG	6 cut(s)	188, 487, 533, 650, 923, 1124
SmoI	CTYRAG	6 cut(s)	188, 487, 533, 650, 923, 1124
SphI	GCATGC	1 cut(s)	410
Sse9I	AATT	1 cut(s)	507
SsiI	CCGC	3 cut(s)	963, 1223, 1326
SstI	GAGCTC	1 cut(s)	1089
StyD4I	CCNGG	5 cut(s)	170, 277, 784, 874, 1012
StyI	CCWWGG	4 cut(s)	384, 951, 1191, 1320
TaaI	ACNGT	5 cut(s)	265, 332, 748, 1052, 1261
TaiI	ACGT	5 cut(s)	208, 763, 900, 939, 1234
TaqI	TCGA	7 cut(s)	48, 62, 126, 240, 588, 1047, 1101
TasI	AATT	1 cut(s)	507
TatI	WGTACW	3 cut(s)	252, 483, 723
TauI	GCSGC	1 cut(s)	1328
TfiI	GAWTC	1 cut(s)	891
Tru1I	TTAA	2 cut(s)	780, 900
Tru9I	TTAA	2 cut(s)	780, 900
TscAI	CASTG	1 cut(s)	268
TseFI	GTSAC	5 cut(s)	74, 287, 593, 796, 1052
TseI	GCWGC	5 cut(s)	137, 450, 453, 974, 1328
Tsp45I	GTSAC	5 cut(s)	74, 287, 593, 796, 1052
TspDTI	ATGAA	3 cut(s)	161, 519, 1019
TspRI	CASTG	1 cut(s)	268
VpaK11BI	GGWCC	2 cut(s)	612, 638
XagI	CCTNNNNNAGG	1 cut(s)	650
XceI	RCATGY	2 cut(s)	410, 1252
XcmI	CCANNNNNNNNNTGG	2 cut(s)	50, 1189
XmiI	GTMKAC	2 cut(s)	48, 62
ZrmI	AGTACT	2 cut(s)	254, 485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.