pycom04g00890

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000682
Physical Location & Seq
Reverse (-)
764711 .. 765034
324 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00890.1

Sequence Viewer

Length: 324 bp
ATGAACAAAAGGTCGTTCAAGTATGCCTGGGTGCTTGACAAGCTTAAGGCCGAGCGTGAGCGTGGTATCACCATTGATATCGCCTTGTGGAAGTTTGAGACCACAAAGTACTATTGCACAGTCATTGATGCTCCGGGGCATCGTGATTTCATTAAGAATATGATTACTGGAACTTCACAGGCTGACTGTGCTGTCCTCATCATTGACTCCACCACTGGTGGTTTTGAAGCTGGTATTTCCAAGGATGGACAGACCCGTGAGCATGCTCTTCTTGCTTTCACCCTCGGTGTGAAGCAGATGATTTGTTGCTGTAACAAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

108

Amino Acids

12.01

Weight (kDa)

8.63

Isoelectric Point (pI)

25.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 8 - 107 8e-33 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AfaI GTAC 1 cut(s) 110
AflII CTTAAG 1 cut(s) 44
AgsI TTSAA 2 cut(s) 19, 227
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 2 cut(s) 43, 230
AluI AGCT 2 cut(s) 43, 230
Alw26I GTCTC 1 cut(s) 92
AoxI GGCC 1 cut(s) 48
AsuC2I CCSGG 1 cut(s) 135
AsuHPI GGTGA 2 cut(s) 61, 271
BccI CCATC 1 cut(s) 239
BciT130I CCWGG 1 cut(s) 28
BcnI CCSGG 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 92
BfrI CTTAAG 1 cut(s) 44
BmcAI AGTACT 1 cut(s) 110
Bme1390I CCNGG 2 cut(s) 28, 135
BmrFI CCNGG 2 cut(s) 28, 135
BmsI GCATC 2 cut(s) 118, 148
BpuMI CCSGG 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 92
BsaJI CCNNGG 4 cut(s) 27, 134, 240, 283
Bse1I ACTGG 2 cut(s) 172, 220
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 4 cut(s) 27, 134, 240, 283
BseGI GGATG 1 cut(s) 250
BseNI ACTGG 2 cut(s) 172, 220
BshFI GGCC 1 cut(s) 50
BsiSI CCGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 92
BsnI GGCC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 92
BspANI GGCC 1 cut(s) 50
BspQI GCTCTTC 1 cut(s) 273
BspTI CTTAAG 1 cut(s) 44
BspTNI GGTCTC 1 cut(s) 92
BsrI ACTGG 2 cut(s) 172, 220
BssECI CCNNGG 4 cut(s) 27, 134, 240, 283
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 2 cut(s) 121, 188
Bst6I CTCTTC 1 cut(s) 273
BstAFI CTTAAG 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 264
BstF5I GGATG 1 cut(s) 250
BstMAI GTCTC 1 cut(s) 92
BstMWI GCNNNNNNNGC 3 cut(s) 40, 188, 272
BstNI CCWGG 1 cut(s) 28
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 2 cut(s) 26, 133
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 264
Csp6I GTAC 1 cut(s) 109
CviAII CATG 1 cut(s) 263
CviJI RGCY 4 cut(s) 43, 50, 182, 230
CviKI_1 RGCY 4 cut(s) 43, 50, 182, 230
CviQI GTAC 1 cut(s) 109
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 92
Eco32I GATATC 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 26
EcoRV GATATC 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 1 cut(s) 266
FaiI YATR 4 cut(s) 24, 161, 264, 322
FatI CATG 1 cut(s) 262
FokI GGATG 1 cut(s) 257
HaeIII GGCC 1 cut(s) 50
HapII CCGG 1 cut(s) 134
Hin1II CATG 1 cut(s) 266
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 1 cut(s) 206
HpaII CCGG 1 cut(s) 134
HphI GGTGA 2 cut(s) 61, 271
Hpy188III TCNNGA 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 121, 188
HpyCH4V TGCA 1 cut(s) 117
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 188, 272
Hsp92II CATG 1 cut(s) 266
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 7 cut(s) 13, 40, 147, 153, 164, 201, 216
LweI GCATC 2 cut(s) 118, 148
MaeIII GTNAC 1 cut(s) 311
MboII GAAGA 1 cut(s) 260
MlyI GAGTC 1 cut(s) 200
MnlI CCTC 2 cut(s) 206, 293
MseI TTAA 2 cut(s) 45, 153
MspCI CTTAAG 1 cut(s) 44
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 2 cut(s) 28, 135
MvaI CCWGG 1 cut(s) 28
MwoI GCNNNNNNNGC 3 cut(s) 40, 188, 272
NciI CCSGG 1 cut(s) 135
NlaIII CATG 1 cut(s) 266
NmeAIII GCCGAG 1 cut(s) 76
NspI RCATGY 1 cut(s) 266
PaeI GCATGC 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 273
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 2 cut(s) 45, 153
ScaI AGTACT 1 cut(s) 110
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 28, 135
SetI ASST 4 cut(s) 14, 45, 232, 321
SfaNI GCATC 2 cut(s) 118, 148
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
SphI GCATGC 1 cut(s) 266
StyD4I CCNGG 2 cut(s) 26, 133
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 2 cut(s) 121, 188
TatI WGTACW 1 cut(s) 108
Tru1I TTAA 2 cut(s) 45, 153
Tru9I TTAA 2 cut(s) 45, 153
TscAI CASTG 1 cut(s) 220
TspDTI ATGAA 2 cut(s) 17, 139
TspRI CASTG 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 44
XceI RCATGY 1 cut(s) 266
ZrmI AGTACT 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.