Rorug04G0129500

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000682
Physical Location & Seq
Forward (+)
20671442 .. 20672555
1114 bp
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UTR
Exon/CDS
Intron
Rorug04G0129500.1

Sequence Viewer

Length: 393 bp
ATGGGACTTGTAATGCAGGTGTTGTTTGTGGTGGTGGCCATGGTTGTATGCCTTCCCTTAGCCGCATACGCGGAACACGGAACTGCAACATTTTATACACCTCCTTACGTTCCCTCTGCATGCAATGGGTATAAAAACGACGGTGTGATGATAGCTGCAGCAAGCAGTGCCATTTGGGACAATAAAGGTGCTTGTGGAAGAAGGTATAGAGTAAAATGCACTGGAGCCACAAACCAAGGTGTGCCAAAACCTTGCAAAGGTAACAGCGTTGTGGTTAAAATCGTGGATTTCTGTCCCCCTGGATGCCATGGAACCATCGATCTCTCTCAAGAAGCCTTTGCTGCCATCGCTAACCCTGATGCTGGCAAAATCAATATTGAGTTCACTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

130

Amino Acids

13.67

Weight (kDa)

7.55

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 52 - 128 1.1e-20 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 7
Acc36I ACCTGC 1 cut(s) 7
AccII CGCG 1 cut(s) 71
AciI CCGC 2 cut(s) 63, 71
AcoI YGGCCR 1 cut(s) 36
AfiI CCNNNNNNNGG 2 cut(s) 257, 362
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 1 cut(s) 155
AluI AGCT 1 cut(s) 155
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 3 cut(s) 155, 158, 341
BalI TGGCCA 1 cut(s) 38
BbvI GCAGC 3 cut(s) 142, 170, 328
BccI CCATC 2 cut(s) 323, 353
BciT130I CCWGG 1 cut(s) 300
BfmI CTRYAG 1 cut(s) 156
BfuAI ACCTGC 1 cut(s) 7
BisI GCNGC 4 cut(s) 63, 156, 159, 342
BlsI GCNGC 4 cut(s) 64, 157, 160, 343
Bme1390I CCNGG 1 cut(s) 300
BmiI GGNNCC 2 cut(s) 226, 313
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 2 cut(s) 293, 349
BpmI CTGGAG 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 58
BpuEI CTTGAG 1 cut(s) 312
Bsa29I ATCGAT 1 cut(s) 318
BsaJI CCNNGG 4 cut(s) 39, 235, 298, 307
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 362
Bse1I ACTGG 1 cut(s) 226
Bse3DI GCAATG 1 cut(s) 130
BseBI CCWGG 1 cut(s) 300
BseCI ATCGAT 1 cut(s) 318
BseDI CCNNGG 4 cut(s) 39, 235, 298, 307
BseGI GGATG 1 cut(s) 308
BseLI CCNNNNNNNGG 2 cut(s) 257, 362
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 1 cut(s) 226
BseXI GCAGC 3 cut(s) 142, 170, 328
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 1 cut(s) 38
BshVI ATCGAT 1 cut(s) 318
BslFI GGGAC 3 cut(s) 18, 191, 279
BslI CCNNNNNNNGG 2 cut(s) 257, 362
BsmFI GGGAC 3 cut(s) 18, 191, 279
BsnI GGCC 1 cut(s) 38
Bsp143I GATC 1 cut(s) 319
Bsp19I CCATGG 2 cut(s) 39, 307
BspACI CCGC 2 cut(s) 63, 71
BspANI GGCC 1 cut(s) 38
BspCNI CTCAG 1 cut(s) 379
BspDI ATCGAT 1 cut(s) 318
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 226, 313
BspMAI CTGCAG 1 cut(s) 160
BspMI ACCTGC 1 cut(s) 7
BsrDI GCAATG 1 cut(s) 130
BsrI ACTGG 1 cut(s) 226
BssECI CCNNGG 4 cut(s) 39, 235, 298, 307
BssMI GATC 1 cut(s) 319
BssT1I CCWWGG 3 cut(s) 39, 235, 307
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 167
BstC8I GCNNGC 3 cut(s) 121, 163, 364
BstDEI CTNAG 2 cut(s) 58, 387
BstDSI CCRYGG 2 cut(s) 39, 307
BstENI CCTNNNNNAGG 1 cut(s) 255
BstF5I GGATG 1 cut(s) 308
BstFNI CGCG 1 cut(s) 71
BstKTI GATC 1 cut(s) 322
BstMBI GATC 1 cut(s) 319
BstMWI GCNNNNNNNGC 4 cut(s) 68, 167, 341, 347
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 1 cut(s) 123
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 156
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 3 cut(s) 142, 170, 328
Bsu15I ATCGAT 1 cut(s) 318
BsuRI GGCC 1 cut(s) 38
BsuTUI ATCGAT 1 cut(s) 318
BtgI CCRYGG 2 cut(s) 39, 307
BtgZI GCGATG 1 cut(s) 331
BtsCI GGATG 1 cut(s) 308
BtsI GCAGTG 1 cut(s) 172
BtsIMutI CAGTG 3 cut(s) 172, 219, 384
BveI ACCTGC 1 cut(s) 7
Cac8I GCNNGC 3 cut(s) 121, 163, 364
ClaI ATCGAT 1 cut(s) 318
CviAII CATG 3 cut(s) 40, 120, 308
CviJI RGCY 5 cut(s) 38, 62, 155, 227, 335
CviKI_1 RGCY 5 cut(s) 38, 62, 155, 227, 335
DdeI CTNAG 2 cut(s) 58, 387
DpnI GATC 1 cut(s) 321
DpnII GATC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 36
Eco130I CCWWGG 3 cut(s) 39, 235, 307
EcoNI CCTNNNNNAGG 1 cut(s) 255
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 3 cut(s) 39, 235, 307
ErhI CCWWGG 3 cut(s) 39, 235, 307
FaeI CATG 3 cut(s) 43, 123, 311
FaiI YATR 8 cut(s) 41, 49, 67, 96, 121, 132, 207, 309
FaqI GGGAC 3 cut(s) 18, 191, 279
FatI CATG 3 cut(s) 39, 119, 307
Fnu4HI GCNGC 4 cut(s) 63, 156, 159, 342
FokI GGATG 1 cut(s) 315
Fsp4HI GCNGC 4 cut(s) 63, 156, 159, 342
GluI GCNGC 4 cut(s) 63, 156, 159, 342
GsuI CTGGAG 1 cut(s) 243
HaeIII GGCC 1 cut(s) 38
Hin1II CATG 3 cut(s) 43, 123, 311
Hpy166II GTNNAC 1 cut(s) 384
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 1 cut(s) 384
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 62, 195
HpyCH4III ACNGT 1 cut(s) 143
HpyCH4IV ACGT 1 cut(s) 108
HpyCH4V TGCA 7 cut(s) 16, 86, 119, 123, 158, 219, 255
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 167, 341, 347
HpyF3I CTNAG 2 cut(s) 58, 387
HpySE526I ACGT 1 cut(s) 108
Hsp92II CATG 3 cut(s) 43, 123, 311
Kzo9I GATC 1 cut(s) 319
LmnI GCTCC 1 cut(s) 224
LpnPI CCDG 6 cut(s) 2, 207, 285, 312, 348, 369
Lsp1109I GCAGC 3 cut(s) 142, 170, 328
LweI GCATC 2 cut(s) 293, 349
MaeII ACGT 1 cut(s) 108
MaeIII GTNAC 1 cut(s) 260
MalI GATC 1 cut(s) 321
MboI GATC 1 cut(s) 319
MboII GAAGA 1 cut(s) 210
MlsI TGGCCA 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 38
MnlI CCTC 2 cut(s) 111, 124
Mox20I TGGCCA 1 cut(s) 38
MscI TGGCCA 1 cut(s) 38
MseI TTAA 1 cut(s) 276
Msp20I TGGCCA 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 4 cut(s) 68, 167, 341, 347
NcoI CCATGG 2 cut(s) 39, 307
NdeII GATC 1 cut(s) 319
NlaIII CATG 3 cut(s) 43, 123, 311
NlaIV GGNNCC 2 cut(s) 226, 313
NspI RCATGY 1 cut(s) 123
PaeI GCATGC 1 cut(s) 123
PaqCI CACCTGC 1 cut(s) 7
PkrI GCNGC 4 cut(s) 64, 157, 160, 343
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 2 cut(s) 226, 313
PstI CTGCAG 1 cut(s) 160
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 4 cut(s) 63, 156, 159, 342
Sau3AI GATC 1 cut(s) 319
ScrFI CCNGG 1 cut(s) 300
SetI ASST 9 cut(s) 21, 103, 111, 157, 190, 206, 241, 253, 262
SfaNI GCATC 2 cut(s) 293, 349
SfcI CTRYAG 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
SphI GCATGC 1 cut(s) 123
SsiI CCGC 2 cut(s) 63, 71
SspI AATATT 1 cut(s) 376
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 3 cut(s) 39, 235, 307
TaaI ACNGT 1 cut(s) 143
TaiI ACGT 1 cut(s) 111
TaqI TCGA 1 cut(s) 318
TauI GCSGC 1 cut(s) 65
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 3 cut(s) 172, 226, 391
TseI GCWGC 3 cut(s) 155, 158, 341
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 3 cut(s) 172, 226, 391
XagI CCTNNNNNAGG 1 cut(s) 255
XceI RCATGY 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.