pycom04g00950

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000682
Physical Location & Seq
Reverse (-)
843907 .. 845636
1730 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00950.2

Sequence Viewer

Length: 1008 bp
ATGGGTAAAGAGAAGTTTCACATCAACATCGTGGTCATTGGCCATGTCGACTCCGGCAAGTCGACCACCACTGGTCATCTTATCTACAAGCTTGGGGGTATTGACAAGCGTGTGATTGAGAGGTTCGAGAAGGAAGCTGCTGAGATGAACAAAAGGTCATTCAAGTATGCCTGGGTGCTTGACAAGCTTAAGGCCGAGCGTGAGCGTGGTATCACCATCGATATTGCCTTGTGGAAGTTTGAGACCACAAAGTACTACTGCACGGTCATTGATGCGCCGGGGCATCGTGATTTCATTAAGAACATGATTACTGGAACTTCACAGGCTGACTGTGCTGTCCTCATCATTGACTCCACCACTGGTGGTTTTGAAGCTGGTATTTCCAAGGACGGACAGACCCGTGAGCACGCTCTTCTTGCTTTCACCCTCGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGATGCCACCACTCCTAAGTACTCTAAGGCAAGGTATGAAGAAATCGTGAAGGAAGTTTCATCCTACCTGAAGAAGGTTGGGTACAACCCTGACAAAATTGCCTTTGTTCCCATCTCTGGGTTTGAGGGTGACAACATGATTGAGAGGTCCACCAACCTTGACTGGTACAAGGGACCAACCCTTCTTGAGGCTCTTGACCTGATCAACGAGCCCAAGAGGCCCTCAGACAAGCCCCTCCGTCTTCCACTTCAGGATGTGTACAAGATCGGTGGTATTGGAACTGTGCCAGTGGGACGTGTTGAGACTGGTATCGTCAAGCCTGGTATGGTTGTGACTTTTGGGCCAACTGGTCTGACAACTGAAGTTAAGTCTGTTGAGATGCACCATGAGGCTCTTCAGGAGGCACTTCCTGGTGACTATGTCGGGTTCAATGTTAAGAATGTTGCTGTGAAGGATCGATCAAGCGTGGTTTCGTCGCATCCAACTCCAAGGATGACCCTGCCAAGGAGGCTGCCAACTTCACATCCCAGGTTATCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05134 map03013 map05134
Pfam Domains
Protein Families
GTP_EFTU GTP_EFTU_D2 GTP_EFTU_D3

Protein Analysis

336

Amino Acids

37.22

Weight (kDa)

8.98

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AccI GTMKAC 2 cut(s) 48, 62
AclWI GGATC 1 cut(s) 927
AcoI YGGCCR 1 cut(s) 40
AcuI CTGAAG 4 cut(s) 554, 698, 845, 846
AfaI GTAC 5 cut(s) 254, 485, 548, 632, 725
AfiI CCNNNNNNNGG 5 cut(s) 538, 581, 582, 652, 969
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 3 cut(s) 163, 371, 895
AjiI CACGTC 1 cut(s) 761
AjnI CCWGG 4 cut(s) 170, 784, 874, 992
AluBI AGCT 4 cut(s) 91, 137, 187, 374
AluI AGCT 4 cut(s) 91, 137, 187, 374
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 2 cut(s) 236, 761
AlwI GGATC 1 cut(s) 927
AoxI GGCC 4 cut(s) 40, 192, 683, 806
ApeKI GCWGC 3 cut(s) 137, 450, 976
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 4 cut(s) 612, 638, 684, 806
AsuC2I CCSGG 1 cut(s) 279
AsuHPI GGTGA 4 cut(s) 205, 415, 605, 890
AvaII GGWCC 2 cut(s) 612, 638
BalI TGGCCA 1 cut(s) 42
BanII GRGCYC 1 cut(s) 678
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BbsI GAAGAC 1 cut(s) 698
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 3 cut(s) 124, 437, 963
BccI CCATC 3 cut(s) 224, 457, 584
BciT130I CCWGG 4 cut(s) 172, 786, 876, 994
BclI TGATCA 1 cut(s) 666
BcnI CCSGG 1 cut(s) 279
BcoDI GTCTC 2 cut(s) 236, 761
BfrI CTTAAG 1 cut(s) 188
BglI GCCNNNNNGGC 2 cut(s) 682, 973
BisI GCNGC 3 cut(s) 138, 451, 977
BlsI GCNGC 3 cut(s) 139, 452, 978
BmcAI AGTACT 2 cut(s) 254, 485
Bme1390I CCNGG 5 cut(s) 172, 279, 786, 876, 994
Bme18I GGWCC 2 cut(s) 612, 638
BmgBI CACGTC 1 cut(s) 761
BmgT120I GGNCC 4 cut(s) 612, 638, 684, 806
BmiI GGNNCC 1 cut(s) 639
BmrFI CCNGG 5 cut(s) 172, 279, 786, 876, 994
BmsI GCATC 5 cut(s) 262, 292, 457, 834, 952
BpiI GAAGAC 1 cut(s) 698
BpuEI CTTGAG 1 cut(s) 671
BpuMI CCSGG 1 cut(s) 279
Bsa29I ATCGAT 2 cut(s) 219, 922
BsaI GGTCTC 1 cut(s) 236
BsaJI CCNNGG 7 cut(s) 171, 278, 384, 427, 953, 968, 992
Bsc4I CCNNNNNNNGG 5 cut(s) 538, 581, 582, 652, 969
Bse1I ACTGG 7 cut(s) 76, 316, 364, 632, 752, 775, 817
BseBI CCWGG 4 cut(s) 172, 786, 876, 994
BseCI ATCGAT 2 cut(s) 219, 922
BseDI CCNNGG 7 cut(s) 171, 278, 384, 427, 953, 968, 992
BseGI GGATG 6 cut(s) 472, 524, 724, 943, 963, 988
BseLI CCNNNNNNNGG 5 cut(s) 538, 581, 582, 652, 969
BseMII CTCAG 2 cut(s) 132, 702
BseNI ACTGG 7 cut(s) 76, 316, 364, 632, 752, 775, 817
BseXI GCAGC 3 cut(s) 124, 437, 963
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 4 cut(s) 42, 194, 685, 808
BshVI ATCGAT 2 cut(s) 219, 922
BsiHKAI GWGCWC 1 cut(s) 408
BsiSI CCGG 2 cut(s) 54, 278
BslFI GGGAC 2 cut(s) 651, 771
BslI CCNNNNNNNGG 5 cut(s) 538, 581, 582, 652, 969
BsmAI GTCTC 2 cut(s) 236, 761
BsmFI GGGAC 2 cut(s) 651, 771
BsnI GGCC 4 cut(s) 42, 194, 685, 808
Bso31I GGTCTC 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 408, 678
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 4 cut(s) 666, 729, 919, 923
BspANI GGCC 4 cut(s) 42, 194, 685, 808
BspCNI CTCAG 2 cut(s) 133, 701
BspDI ATCGAT 2 cut(s) 219, 922
BspHI TCATGA 1 cut(s) 1004
BspLI GGNNCC 1 cut(s) 639
BspPI GGATC 1 cut(s) 927
BspQI GCTCTTC 2 cut(s) 417, 864
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 236
BsrGI TGTACA 1 cut(s) 723
BsrI ACTGG 7 cut(s) 76, 316, 364, 632, 752, 775, 817
BssECI CCNNGG 7 cut(s) 171, 278, 384, 427, 953, 968, 992
BssMI GATC 4 cut(s) 666, 729, 919, 923
BssT1I CCWWGG 3 cut(s) 384, 953, 968
Bst2UI CCWGG 4 cut(s) 172, 786, 876, 994
Bst4CI ACNGT 3 cut(s) 265, 332, 748
Bst6I CTCTTC 2 cut(s) 417, 864
BstAFI CTTAAG 1 cut(s) 188
BstAUI TGTACA 1 cut(s) 723
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 4 cut(s) 141, 480, 489, 688
BstENI CCTNNNNNAGG 2 cut(s) 536, 650
BstF5I GGATG 6 cut(s) 472, 524, 724, 943, 963, 988
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 4 cut(s) 669, 732, 922, 926
BstMAI GTCTC 2 cut(s) 236, 761
BstMBI GATC 4 cut(s) 666, 729, 919, 923
BstMWI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 973
BstNI CCWGG 4 cut(s) 172, 786, 876, 994
BstSCI CCNGG 5 cut(s) 170, 277, 784, 874, 992
BstV1I GCAGC 3 cut(s) 124, 437, 963
BstV2I GAAGAC 1 cut(s) 698
Bsu15I ATCGAT 2 cut(s) 219, 922
BsuRI GGCC 4 cut(s) 42, 194, 685, 808
BsuTUI ATCGAT 2 cut(s) 219, 922
BtrI CACGTC 1 cut(s) 761
BtsCI GGATG 6 cut(s) 472, 524, 724, 943, 963, 988
BtsIMutI CAGTG 3 cut(s) 69, 357, 759
Cac8I GCNNGC 1 cut(s) 408
CciI TCATGA 1 cut(s) 1004
CfoI GCGC 1 cut(s) 277
Cfr13I GGNCC 4 cut(s) 612, 638, 684, 806
ClaI ATCGAT 2 cut(s) 219, 922
Csp6I GTAC 5 cut(s) 253, 484, 547, 631, 724
CspCI CAANNNNNGTGG 4 cut(s) 604, 639, 715, 750
CviAII CATG 5 cut(s) 44, 304, 601, 851, 1005
CviQI GTAC 5 cut(s) 253, 484, 547, 631, 724
DdeI CTNAG 4 cut(s) 141, 480, 489, 688
DpnI GATC 4 cut(s) 668, 731, 921, 925
DpnII GATC 4 cut(s) 666, 729, 919, 923
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 40
Eam1104I CTCTTC 2 cut(s) 417, 864
EarI CTCTTC 2 cut(s) 417, 864
Eco130I CCWWGG 3 cut(s) 384, 953, 968
Eco24I GRGCYC 1 cut(s) 678
Eco31I GGTCTC 1 cut(s) 236
Eco47I GGWCC 2 cut(s) 612, 638
Eco57I CTGAAG 4 cut(s) 554, 698, 845, 846
EcoNI CCTNNNNNAGG 2 cut(s) 536, 650
EcoO109I RGGNCCY 1 cut(s) 684
EcoRII CCWGG 4 cut(s) 170, 784, 874, 992
EcoT14I CCWWGG 3 cut(s) 384, 953, 968
EcoT38I GRGCYC 1 cut(s) 678
ErhI CCWWGG 3 cut(s) 384, 953, 968
FaeI CATG 5 cut(s) 47, 307, 604, 854, 1008
FaiI YATR 9 cut(s) 45, 168, 305, 501, 602, 791, 852, 885, 1006
FaqI GGGAC 2 cut(s) 651, 771
FatI CATG 5 cut(s) 43, 303, 600, 850, 1004
FbaI TGATCA 1 cut(s) 666
FblI GTMKAC 2 cut(s) 48, 62
Fnu4HI GCNGC 3 cut(s) 138, 451, 977
FokI GGATG 6 cut(s) 479, 511, 731, 930, 970, 975
FriOI GRGCYC 1 cut(s) 678
Fsp4HI GCNGC 3 cut(s) 138, 451, 977
GlaI GCGC 1 cut(s) 276
GluI GCNGC 3 cut(s) 138, 451, 977
HaeIII GGCC 4 cut(s) 42, 194, 685, 808
HapII CCGG 2 cut(s) 54, 278
HhaI GCGC 1 cut(s) 277
Hin1II CATG 5 cut(s) 47, 307, 604, 854, 1008
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HincII GTYRAC 2 cut(s) 49, 63
HindII GTYRAC 2 cut(s) 49, 63
HindIII AAGCTT 2 cut(s) 89, 185
HinfI GANTC 2 cut(s) 50, 350
HpaII CCGG 2 cut(s) 54, 278
HphI GGTGA 4 cut(s) 205, 415, 605, 890
Hpy166II GTNNAC 4 cut(s) 49, 63, 615, 724
Hpy188I TCNGA 2 cut(s) 691, 819
Hpy188III TCNNGA 8 cut(s) 127, 287, 511, 650, 659, 716, 863, 1005
Hpy8I GTNNAC 4 cut(s) 49, 63, 615, 724
Hpy99I CGWCG 1 cut(s) 943
HpyAV CCTTC 5 cut(s) 124, 508, 532, 656, 910
HpyCH4III ACNGT 3 cut(s) 265, 332, 748
HpyCH4IV ACGT 1 cut(s) 760
HpyCH4V TGCA 2 cut(s) 261, 847
HpyF10VI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 973
HpyF3I CTNAG 4 cut(s) 141, 480, 489, 688
HpySE526I ACGT 1 cut(s) 760
Hsp92II CATG 5 cut(s) 47, 307, 604, 854, 1008
HspAI GCGC 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 4 cut(s) 666, 729, 919, 923
LguI GCTCTTC 2 cut(s) 417, 864
Lsp1109I GCAGC 3 cut(s) 124, 437, 963
LweI GCATC 5 cut(s) 262, 292, 457, 834, 952
MaeII ACGT 1 cut(s) 760
MaeIII GTNAC 4 cut(s) 455, 593, 796, 878
MalI GATC 4 cut(s) 668, 731, 921, 925
MboI GATC 4 cut(s) 666, 729, 919, 923
MboII GAAGA 5 cut(s) 404, 515, 547, 698, 851
MhlI GDGCHC 2 cut(s) 408, 678
MlsI TGGCCA 1 cut(s) 42
MluCI AATT 1 cut(s) 561
MluNI TGGCCA 1 cut(s) 42
MlyI GAGTC 2 cut(s) 44, 344
MmeI TCCRAC 1 cut(s) 971
Mox20I TGGCCA 1 cut(s) 42
MscI TGGCCA 1 cut(s) 42
MseI TTAA 4 cut(s) 189, 297, 831, 900
Msp20I TGGCCA 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 188
MspI CCGG 2 cut(s) 54, 278
MspR9I CCNGG 5 cut(s) 172, 279, 786, 876, 994
MvaI CCWGG 4 cut(s) 172, 786, 876, 994
MwoI GCNNNNNNNGC 5 cut(s) 184, 332, 416, 682, 973
NciI CCSGG 1 cut(s) 279
NdeII GATC 4 cut(s) 666, 729, 919, 923
NlaIII CATG 5 cut(s) 47, 307, 604, 854, 1008
NlaIV GGNNCC 1 cut(s) 639
NmeAIII GCCGAG 1 cut(s) 220
NmuCI GTSAC 3 cut(s) 593, 796, 878
PagI TCATGA 1 cut(s) 1004
PciSI GCTCTTC 2 cut(s) 417, 864
PflFI GACNNNGTC 1 cut(s) 884
PkrI GCNGC 3 cut(s) 139, 452, 978
PleI GAGTC 2 cut(s) 44, 344
PpsI GAGTC 2 cut(s) 44, 344
Psp6I CCWGG 4 cut(s) 170, 784, 874, 992
PspGI CCWGG 4 cut(s) 170, 784, 874, 992
PspN4I GGNNCC 1 cut(s) 639
PspPI GGNCC 4 cut(s) 612, 638, 684, 806
PsyI GACNNNGTC 1 cut(s) 884
RsaI GTAC 5 cut(s) 254, 485, 548, 632, 725
RsaNI GTAC 5 cut(s) 253, 484, 547, 631, 724
SalI GTCGAC 2 cut(s) 47, 61
SapI GCTCTTC 2 cut(s) 417, 864
SaqAI TTAA 4 cut(s) 189, 297, 831, 900
SatI GCNGC 3 cut(s) 138, 451, 977
Sau3AI GATC 4 cut(s) 666, 729, 919, 923
Sau96I GGNCC 4 cut(s) 612, 638, 684, 806
ScaI AGTACT 2 cut(s) 254, 485
SchI GAGTC 2 cut(s) 44, 344
ScrFI CCNGG 5 cut(s) 172, 279, 786, 876, 994
SduI GDGCHC 2 cut(s) 408, 678
SfaNI GCATC 5 cut(s) 262, 292, 457, 834, 952
SinI GGWCC 2 cut(s) 612, 638
SmlI CTYRAG 2 cut(s) 188, 650
SmoI CTYRAG 2 cut(s) 188, 650
Sse9I AATT 1 cut(s) 561
StyD4I CCNGG 5 cut(s) 170, 277, 784, 874, 992
StyI CCWWGG 3 cut(s) 384, 953, 968
TaaI ACNGT 3 cut(s) 265, 332, 748
TaiI ACGT 1 cut(s) 763
TaqI TCGA 5 cut(s) 48, 62, 126, 219, 922
TasI AATT 1 cut(s) 561
TatI WGTACW 3 cut(s) 252, 483, 723
Tru1I TTAA 4 cut(s) 189, 297, 831, 900
Tru9I TTAA 4 cut(s) 189, 297, 831, 900
TscAI CASTG 3 cut(s) 76, 364, 759
TseFI GTSAC 3 cut(s) 593, 796, 878
TseI GCWGC 3 cut(s) 137, 450, 976
Tsp45I GTSAC 3 cut(s) 593, 796, 878
TspDTI ATGAA 4 cut(s) 161, 283, 513, 516
TspGWI ACGGA 2 cut(s) 405, 692
TspRI CASTG 3 cut(s) 76, 364, 759
Tth111I GACNNNGTC 1 cut(s) 884
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 2 cut(s) 612, 638
XagI CCTNNNNNAGG 2 cut(s) 536, 650
XmiI GTMKAC 2 cut(s) 48, 62
ZrmI AGTACT 2 cut(s) 254, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.