FvH4_3g34730

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002234
Physical Location & Seq
Reverse (-)
30061686 .. 30063234
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34730.t1

Sequence Viewer

Length: 372 bp
ATGGCGAGGATCAAGGTGCACGAGCTGAGGAACAAGTCCAAGGCAGATCTTTTGGGCCAGCTCAAGGATCTCAAGGCGGAGCTCGCTCTTCTCCGAGTCGCCAAGGTCACTGGTGGAGCCCCCAACAAGCTCTCCAAAATCAAGGTTGTGAGGCTTTCAATTGCTCAGGTGTTGACAGTGATTTCCCAGAAGCAGAAGGCGGCTCTTAGGGAAGTTTACAAGAACAAGAAGCTTCTTCCTCTTGATTTGCGTCCGAAGAAGACCAGGGCCATCAGGAGGCGTCTGACTAAGCACCAGGCATCTTTAAAGACCGAGAGGGAGAAGAAAAGAGAGATGTACTTCCCACTAAGGAAGTACGCAATCAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L29

Protein Analysis

124

Amino Acids

14.19

Weight (kDa)

11.16

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 8 - 64 6.6e-18 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 77, 200
AclWI GGATC 2 cut(s) 17, 75
AcyI GRCGYC 1 cut(s) 280
AfaI GTAC 2 cut(s) 338, 356
AfiI CCNNNNNNNGG 2 cut(s) 64, 276
AgsI TTSAA 1 cut(s) 159
AjnI CCWGG 2 cut(s) 263, 294
AluBI AGCT 5 cut(s) 25, 61, 82, 130, 232
AluI AGCT 5 cut(s) 25, 61, 82, 130, 232
Alw21I GWGCWC 2 cut(s) 21, 84
Alw44I GTGCAC 1 cut(s) 17
AlwI GGATC 2 cut(s) 17, 75
AoxI GGCC 2 cut(s) 55, 267
ApaLI GTGCAC 1 cut(s) 17
AspS9I GGNCC 2 cut(s) 55, 267
BaeGI GKGCMC 1 cut(s) 21
BanII GRGCYC 2 cut(s) 84, 121
BauI CACGAG 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 266
Bbv12I GWGCWC 2 cut(s) 21, 84
BbvCI CCTCAGC 1 cut(s) 26
BccI CCATC 1 cut(s) 278
BciT130I CCWGG 2 cut(s) 265, 296
BglII AGATCT 1 cut(s) 46
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 2 cut(s) 265, 296
BmgT120I GGNCC 2 cut(s) 55, 267
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 2 cut(s) 265, 296
BmsI GCATC 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 266
Bpu10I CCTNAGC 2 cut(s) 26, 165
BpuEI CTTGAG 2 cut(s) 47, 56
BsaHI GRCGYC 1 cut(s) 280
BsaJI CCNNGG 3 cut(s) 39, 102, 264
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 276
Bse1I ACTGG 1 cut(s) 115
BseBI CCWGG 2 cut(s) 265, 296
BseDI CCNNGG 3 cut(s) 39, 102, 264
BseLI CCNNNNNNNGG 2 cut(s) 64, 276
BseMII CTCAG 2 cut(s) 17, 179
BseNI ACTGG 1 cut(s) 115
BseSI GKGCMC 1 cut(s) 21
BshFI GGCC 2 cut(s) 57, 269
BsiHKAI GWGCWC 2 cut(s) 21, 84
BslI CCNNNNNNNGG 2 cut(s) 64, 276
BsnI GGCC 2 cut(s) 57, 269
Bsp1286I GDGCHC 3 cut(s) 21, 84, 121
Bsp143I GATC 3 cut(s) 9, 46, 67
BspACI CCGC 2 cut(s) 77, 200
BspANI GGCC 2 cut(s) 57, 269
BspCNI CTCAG 2 cut(s) 18, 178
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 2 cut(s) 17, 75
BspQI GCTCTTC 1 cut(s) 93
BsrI ACTGG 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 39, 102, 264
BssMI GATC 3 cut(s) 9, 46, 67
BssNI GRCGYC 1 cut(s) 280
BssSI CACGAG 1 cut(s) 20
BssT1I CCWWGG 2 cut(s) 39, 102
Bst2BI CACGAG 1 cut(s) 20
Bst2UI CCWGG 2 cut(s) 265, 296
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 280
BstC8I GCNNGC 2 cut(s) 59, 84
BstDEI CTNAG 5 cut(s) 26, 165, 206, 288, 347
BstKTI GATC 3 cut(s) 12, 49, 70
BstMBI GATC 3 cut(s) 9, 46, 67
BstMWI GCNNNNNNNGC 2 cut(s) 83, 365
BstNI CCWGG 2 cut(s) 265, 296
BstSCI CCNGG 2 cut(s) 263, 294
BstSLI GKGCMC 1 cut(s) 21
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 2 cut(s) 46, 67
BstYI RGATCY 2 cut(s) 46, 67
BsuRI GGCC 2 cut(s) 57, 269
BtsIMutI CAGTG 2 cut(s) 108, 183
Cac8I GCNNGC 2 cut(s) 59, 84
Cfr13I GGNCC 2 cut(s) 55, 267
CseI GACGC 2 cut(s) 239, 269
Csp6I GTAC 2 cut(s) 337, 355
CviAII CATG 1 cut(s) 369
CviQI GTAC 2 cut(s) 337, 355
DdeI CTNAG 5 cut(s) 26, 165, 206, 288, 347
DpnI GATC 3 cut(s) 11, 48, 69
DpnII GATC 3 cut(s) 9, 46, 67
DraI TTTAAA 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 2 cut(s) 39, 102
Eco24I GRGCYC 2 cut(s) 84, 121
Eco53kI GAGCTC 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 263, 294
EcoT14I CCWWGG 2 cut(s) 39, 102
EcoT38I GRGCYC 2 cut(s) 84, 121
ErhI CCWWGG 2 cut(s) 39, 102
FaeI CATG 1 cut(s) 372
FaiI YATR 1 cut(s) 370
FatI CATG 1 cut(s) 368
Fnu4HI GCNGC 1 cut(s) 201
FriOI GRGCYC 2 cut(s) 84, 121
Fsp4HI GCNGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 2 cut(s) 57, 269
HgaI GACGC 2 cut(s) 239, 269
Hin1I GRCGYC 1 cut(s) 280
Hin1II CATG 1 cut(s) 372
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 1 cut(s) 96
Hpy166II GTNNAC 3 cut(s) 19, 174, 217
Hpy188I TCNGA 3 cut(s) 95, 255, 285
Hpy188III TCNNGA 2 cut(s) 242, 274
Hpy8I GTNNAC 3 cut(s) 19, 174, 217
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4V TGCA 1 cut(s) 19
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 365
HpyF3I CTNAG 5 cut(s) 26, 165, 206, 288, 347
Hsp92I GRCGYC 1 cut(s) 280
Hsp92II CATG 1 cut(s) 372
Kzo9I GATC 3 cut(s) 9, 46, 67
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 2 cut(s) 79, 116
LpnPI CCDG 9 cut(s) 71, 96, 152, 200, 250, 259, 277, 281, 308
LweI GCATC 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 106
MalI GATC 3 cut(s) 11, 48, 69
MboI GATC 3 cut(s) 9, 46, 67
MboII GAAGA 5 cut(s) 80, 227, 268, 271, 334
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 2 cut(s) 46, 67
MhlI GDGCHC 3 cut(s) 21, 84, 121
MluCI AATT 1 cut(s) 159
MlyI GAGTC 1 cut(s) 105
MnlI CCTC 5 cut(s) 21, 144, 249, 270, 309
MseI TTAA 1 cut(s) 305
MspR9I CCNGG 2 cut(s) 265, 296
MunI CAATTG 1 cut(s) 159
MvaI CCWGG 2 cut(s) 265, 296
MwoI GCNNNNNNNGC 2 cut(s) 83, 365
NdeII GATC 3 cut(s) 9, 46, 67
NlaIII CATG 1 cut(s) 372
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 93
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 2 cut(s) 263, 294
PspGI CCWGG 2 cut(s) 263, 294
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 2 cut(s) 55, 267
PsuI RGATCY 2 cut(s) 46, 67
RsaI GTAC 2 cut(s) 338, 356
RsaNI GTAC 2 cut(s) 337, 355
SacI GAGCTC 1 cut(s) 84
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 3 cut(s) 9, 46, 67
Sau96I GGNCC 2 cut(s) 55, 267
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 2 cut(s) 265, 296
SduI GDGCHC 3 cut(s) 21, 84, 121
SetI ASST 9 cut(s) 18, 27, 63, 84, 108, 132, 147, 171, 234
SfaNI GCATC 1 cut(s) 308
SmlI CTYRAG 2 cut(s) 62, 71
SmoI CTYRAG 2 cut(s) 62, 71
Sse9I AATT 1 cut(s) 159
SsiI CCGC 2 cut(s) 77, 200
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 263, 294
StyI CCWWGG 2 cut(s) 39, 102
TaaI ACNGT 1 cut(s) 178
TaqII GACCGA 1 cut(s) 326
TasI AATT 1 cut(s) 159
TatI WGTACW 1 cut(s) 336
TauI GCSGC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 2 cut(s) 115, 183
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspRI CASTG 2 cut(s) 115, 183
VneI GTGCAC 1 cut(s) 17
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.