MD03G1102300.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002234
Physical Location & Seq
Forward (+)
8751810 .. 8753993
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1102300.v1.1.491

Sequence Viewer

Length: 372 bp
ATGGCGAGGATTAAGGTCCATGAACTCAGGCACAAAACCAAGGCTGATCTTTTGGCTCAGCTGAAGGATCTGAAGGCAGAGCTCGCTCTTCTCCGAGTCGCCAAGGTCACCGGCGGAGCCCCTAACAAGCTCTCCAAAATCAAGGTGGTGAGGCTTTCAATCGCTCAGGTGTTGACGGTGATGTCTCAGAAGCAGAAGGCGGCTCTGAGGGAAGTCTACAGGAATAAGAAGCTTCTTCCTCTCGATTTGCGTCCCAAGAAGACTAGGGCCATCAGGAGGCGTCTCACCAAGCACCAGGCATCTTTGAAGACAGAAAGAGAGAAGAAAAAGGAGATCTACTACCCATTGAGGAAGTACGCAATCAAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L29

Protein Analysis

124

Amino Acids

14.26

Weight (kDa)

11.02

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 8 - 64 2.3e-17 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
AccI GTMKAC 1 cut(s) 216
AciI CCGC 2 cut(s) 114, 200
AclWI GGATC 1 cut(s) 75
AcuI CTGAAG 2 cut(s) 83, 92
AcyI GRCGYC 1 cut(s) 280
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 276
AgsI TTSAA 2 cut(s) 159, 307
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 4 cut(s) 61, 82, 130, 232
AluI AGCT 4 cut(s) 61, 82, 130, 232
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 2 cut(s) 189, 287
AlwI GGATC 1 cut(s) 75
AoxI GGCC 1 cut(s) 267
AspS9I GGNCC 2 cut(s) 16, 267
AsuHPI GGTGA 4 cut(s) 100, 160, 190, 277
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 2 cut(s) 84, 121
BbsI GAAGAC 2 cut(s) 266, 314
Bbv12I GWGCWC 1 cut(s) 84
BccI CCATC 1 cut(s) 278
BciT130I CCWGG 1 cut(s) 296
BcoDI GTCTC 2 cut(s) 189, 287
BfaI CTAG 1 cut(s) 264
BfmI CTRYAG 1 cut(s) 217
BglII AGATCT 1 cut(s) 333
BisI GCNGC 1 cut(s) 201
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 1 cut(s) 296
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 16, 267
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 1 cut(s) 296
BmsI GCATC 1 cut(s) 308
BpiI GAAGAC 2 cut(s) 266, 314
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 57
BsaHI GRCGYC 1 cut(s) 280
BsaJI CCNNGG 2 cut(s) 39, 102
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse118I RCCGGY 1 cut(s) 110
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 2 cut(s) 39, 102
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMII CTCAG 5 cut(s) 40, 71, 179, 197, 200
BshFI GGCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 1 cut(s) 111
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 276
BsmAI GTCTC 2 cut(s) 189, 287
BsmBI CGTCTC 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 237
BsnI GGCC 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 84, 121
Bsp143I GATC 3 cut(s) 46, 67, 333
Bsp1720I GCTNAGC 1 cut(s) 57
BspACI CCGC 2 cut(s) 114, 200
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 5 cut(s) 39, 70, 178, 198, 199
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 1 cut(s) 75
BspQI GCTCTTC 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 39, 102
BssMI GATC 3 cut(s) 46, 67, 333
BssNI GRCGYC 1 cut(s) 280
BssT1I CCWWGG 2 cut(s) 39, 102
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 5 cut(s) 26, 57, 165, 186, 206
BstEII GGTNACC 1 cut(s) 106
BstKTI GATC 3 cut(s) 49, 70, 336
BstMAI GTCTC 2 cut(s) 189, 287
BstMBI GATC 3 cut(s) 46, 67, 333
BstMWI GCNNNNNNNGC 2 cut(s) 83, 365
BstNI CCWGG 1 cut(s) 296
BstPI GGTNACC 1 cut(s) 106
BstSCI CCNGG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 217
BstV2I GAAGAC 2 cut(s) 266, 314
BstX2I RGATCY 2 cut(s) 67, 333
BstYI RGATCY 2 cut(s) 67, 333
BsuRI GGCC 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 84
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 16, 267
CseI GACGC 2 cut(s) 239, 269
Csp6I GTAC 1 cut(s) 355
CviAII CATG 1 cut(s) 20
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 5 cut(s) 26, 57, 165, 186, 206
DpnI GATC 3 cut(s) 48, 69, 335
DpnII GATC 3 cut(s) 46, 67, 333
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
EciI GGCGGA 1 cut(s) 129
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 2 cut(s) 39, 102
Eco24I GRGCYC 2 cut(s) 84, 121
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 2 cut(s) 83, 92
Eco91I GGTNACC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 82
EcoO65I GGTNACC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 2 cut(s) 39, 102
EcoT38I GRGCYC 2 cut(s) 84, 121
ErhI CCWWGG 2 cut(s) 39, 102
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 1 cut(s) 23
FaiI YATR 2 cut(s) 21, 370
FaqI GGGAC 1 cut(s) 237
FatI CATG 1 cut(s) 19
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 1 cut(s) 201
FriOI GRGCYC 2 cut(s) 84, 121
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 1 cut(s) 264
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 1 cut(s) 269
HapII CCGG 1 cut(s) 111
HgaI GACGC 2 cut(s) 239, 269
Hin1I GRCGYC 1 cut(s) 280
Hin1II CATG 1 cut(s) 23
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 1 cut(s) 96
HpaII CCGG 1 cut(s) 111
HphI GGTGA 4 cut(s) 100, 160, 190, 277
Hpy166II GTNNAC 2 cut(s) 174, 217
Hpy188I TCNGA 4 cut(s) 72, 95, 189, 207
Hpy188III TCNNGA 2 cut(s) 242, 274
Hpy8I GTNNAC 2 cut(s) 174, 217
HpyAV CCTTC 3 cut(s) 58, 67, 190
HpyCH4III ACNGT 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 365
HpyF3I CTNAG 5 cut(s) 26, 57, 165, 186, 206
Hsp92I GRCGYC 1 cut(s) 280
Hsp92II CATG 1 cut(s) 23
Kzo9I GATC 3 cut(s) 46, 67, 333
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 1 cut(s) 116
LpnPI CCDG 7 cut(s) 13, 124, 152, 205, 259, 281, 308
LweI GCATC 1 cut(s) 308
MaeI CTAG 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 106
MalI GATC 3 cut(s) 48, 69, 335
MboI GATC 3 cut(s) 46, 67, 333
MboII GAAGA 5 cut(s) 80, 227, 271, 319, 334
MflI RGATCY 2 cut(s) 67, 333
MhlI GDGCHC 2 cut(s) 84, 121
MlyI GAGTC 1 cut(s) 105
MnlI CCTC 5 cut(s) 144, 201, 249, 270, 342
MseI TTAA 1 cut(s) 12
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 1 cut(s) 111
MspR9I CCNGG 1 cut(s) 296
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 2 cut(s) 83, 365
NdeII GATC 3 cut(s) 46, 67, 333
NlaIII CATG 1 cut(s) 23
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 93
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 1 cut(s) 294
PspEI GGTNACC 1 cut(s) 106
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 2 cut(s) 16, 267
PsuI RGATCY 2 cut(s) 67, 333
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
SacI GAGCTC 1 cut(s) 84
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 3 cut(s) 46, 67, 333
Sau96I GGNCC 2 cut(s) 16, 267
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 1 cut(s) 296
SduI GDGCHC 2 cut(s) 84, 121
SetI ASST 8 cut(s) 18, 63, 84, 108, 132, 147, 171, 234
SfaNI GCATC 1 cut(s) 308
SfcI CTRYAG 1 cut(s) 217
SgrAI CRCCGGYG 1 cut(s) 110
SinI GGWCC 1 cut(s) 16
SsiI CCGC 2 cut(s) 114, 200
SspMI CTAG 1 cut(s) 264
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 1 cut(s) 294
StyI CCWWGG 2 cut(s) 39, 102
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 1 cut(s) 243
TauI GCSGC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 16
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.