MD07G1188600.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002234
Physical Location & Seq
Reverse (-)
26927605 .. 26928763
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1188600.v1.1.491

Sequence Viewer

Length: 372 bp
ATGGCGAGAATCAAGGTACATGAGCTGAGGCACAAGACAAAGGCTGACCTTTTGGCTCAGCTCAAGGATCTCAAGGCGGAGCTCGCCCTCCTCCGCGTCGCTAAGGTCACCGGCGGAGCGCCCAACAAGCTCTCCAAGATCAAGGTTGTGAGGCTGTCGATCGCCCAGGTGTTGACGGTGATGTCACAGAAGCAGAAGGCTGCTCTCAGAGAAGTGTACAGGAACAAGAAGCTCTTGCCTCTTGATCTCCGTCCCAAGAAGACCAGGGCCATCCGCCGGAGGCTCACCAAGCACCAGTCTTCTTTGAAGACCGAGAGGGAGAAGAAGAAGGAGATGTACTACCCTTTGAGGAAGTATGCCATTAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L29

Protein Analysis

124

Amino Acids

14.29

Weight (kDa)

11.02

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 8 - 64 2.3e-17 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
AccII CGCG 1 cut(s) 96
AciI CCGC 4 cut(s) 77, 94, 114, 274
AclWI GGATC 1 cut(s) 75
AfaI GTAC 3 cut(s) 18, 218, 338
AfiI CCNNNNNNNGG 1 cut(s) 276
AgsI TTSAA 1 cut(s) 307
AjnI CCWGG 2 cut(s) 165, 263
AluBI AGCT 5 cut(s) 25, 61, 82, 130, 232
AluI AGCT 5 cut(s) 25, 61, 82, 130, 232
Alw21I GWGCWC 1 cut(s) 84
AlwI GGATC 1 cut(s) 75
AoxI GGCC 1 cut(s) 267
ApeKI GCWGC 1 cut(s) 200
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 1 cut(s) 267
AsuHPI GGTGA 3 cut(s) 100, 190, 277
BanII GRGCYC 1 cut(s) 84
BarI GAAGNNNNNNTAC 2 cut(s) 320, 352
BbsI GAAGAC 3 cut(s) 266, 291, 314
Bbv12I GWGCWC 1 cut(s) 84
BbvCI CCTCAGC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 187
BccI CCATC 1 cut(s) 278
BciT130I CCWGG 2 cut(s) 167, 265
BfoI RGCGCY 1 cut(s) 122
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 1 cut(s) 201
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 2 cut(s) 167, 265
BmgT120I GGNCC 1 cut(s) 267
BmrFI CCNGG 2 cut(s) 167, 265
BpiI GAAGAC 3 cut(s) 266, 291, 314
Bpu10I CCTNAGC 2 cut(s) 26, 102
Bpu1102I GCTNAGC 1 cut(s) 57
BpuEI CTTGAG 2 cut(s) 47, 56
BsaJI CCNNGG 2 cut(s) 165, 264
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 1 cut(s) 295
BseBI CCWGG 2 cut(s) 167, 265
BseDI CCNNGG 2 cut(s) 165, 264
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMII CTCAG 3 cut(s) 17, 71, 220
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 1 cut(s) 269
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 2 cut(s) 111, 277
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 237
BsnI GGCC 1 cut(s) 269
Bsp1286I GDGCHC 1 cut(s) 84
Bsp1407I TGTACA 1 cut(s) 216
Bsp143I GATC 4 cut(s) 67, 138, 159, 244
Bsp1720I GCTNAGC 1 cut(s) 57
BspACI CCGC 4 cut(s) 77, 94, 114, 274
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 3 cut(s) 18, 70, 219
BspFNI CGCG 1 cut(s) 96
BspPI GGATC 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 110
BsrGI TGTACA 1 cut(s) 216
BsrI ACTGG 1 cut(s) 295
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 165, 264
BssMI GATC 4 cut(s) 67, 138, 159, 244
Bst2UI CCWGG 2 cut(s) 167, 265
Bst4CI ACNGT 1 cut(s) 178
BstAUI TGTACA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 5 cut(s) 26, 57, 102, 206, 369
BstEII GGTNACC 1 cut(s) 106
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 4 cut(s) 70, 141, 162, 247
BstMBI GATC 4 cut(s) 67, 138, 159, 244
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 4 cut(s) 83, 127, 289, 365
BstNI CCWGG 2 cut(s) 167, 265
BstPI GGTNACC 1 cut(s) 106
BstSCI CCNGG 2 cut(s) 165, 263
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 187
BstV2I GAAGAC 3 cut(s) 266, 291, 314
BstX2I RGATCY 1 cut(s) 67
BstYI RGATCY 1 cut(s) 67
BsuRI GGCC 1 cut(s) 269
BtsCI GGATG 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 84
CfoI GCGC 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 1 cut(s) 267
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 17, 217, 337
CviAII CATG 1 cut(s) 20
CviQI GTAC 3 cut(s) 17, 217, 337
DdeI CTNAG 5 cut(s) 26, 57, 102, 206, 369
DpnI GATC 4 cut(s) 69, 140, 161, 246
DpnII GATC 4 cut(s) 67, 138, 159, 244
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
EciI GGCGGA 3 cut(s) 92, 129, 263
Ecl136II GAGCTC 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
Eco91I GGTNACC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 82
EcoO65I GGTNACC 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 165, 263
EcoT38I GRGCYC 1 cut(s) 84
FaeI CATG 1 cut(s) 23
FaiI YATR 2 cut(s) 21, 357
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 237
FatI CATG 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 201
GlaI GCGC 1 cut(s) 120
GluI GCNGC 1 cut(s) 201
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 1 cut(s) 269
HapII CCGG 2 cut(s) 111, 277
HgaI GACGC 1 cut(s) 85
HhaI GCGC 1 cut(s) 121
Hin1II CATG 1 cut(s) 23
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HinfI GANTC 1 cut(s) 9
HpaII CCGG 2 cut(s) 111, 277
HphI GGTGA 3 cut(s) 100, 190, 277
Hpy166II GTNNAC 2 cut(s) 174, 217
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 1 cut(s) 242
Hpy8I GTNNAC 2 cut(s) 174, 217
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 2 cut(s) 190, 322
HpyCH4III ACNGT 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 127, 289, 365
HpyF3I CTNAG 5 cut(s) 26, 57, 102, 206, 369
Hsp92II CATG 1 cut(s) 23
HspAI GCGC 1 cut(s) 119
Kzo9I GATC 4 cut(s) 67, 138, 159, 244
LmnI GCTCC 2 cut(s) 79, 116
LpnPI CCDG 8 cut(s) 124, 152, 179, 205, 250, 277, 290, 308
Lsp1109I GCAGC 1 cut(s) 187
MaeIII GTNAC 2 cut(s) 106, 183
MalI GATC 4 cut(s) 69, 140, 161, 246
MboI GATC 4 cut(s) 67, 138, 159, 244
MboII GAAGA 5 cut(s) 271, 291, 319, 334, 337
MflI RGATCY 1 cut(s) 67
MhlI GDGCHC 1 cut(s) 84
MnlI CCTC 8 cut(s) 21, 98, 101, 144, 249, 273, 309, 342
MseI TTAA 1 cut(s) 363
MspI CCGG 2 cut(s) 111, 277
MspR9I CCNGG 2 cut(s) 167, 265
MvaI CCWGG 2 cut(s) 167, 265
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 4 cut(s) 83, 127, 289, 365
NdeII GATC 4 cut(s) 67, 138, 159, 244
NlaIII CATG 1 cut(s) 23
NmuCI GTSAC 2 cut(s) 106, 183
PfeI GAWTC 1 cut(s) 9
PkrI GCNGC 1 cut(s) 202
Ple19I CGATCG 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 2 cut(s) 165, 263
PspEI GGTNACC 1 cut(s) 106
PspGI CCWGG 2 cut(s) 165, 263
PspPI GGNCC 1 cut(s) 267
PsuI RGATCY 1 cut(s) 67
PvuI CGATCG 1 cut(s) 162
RsaI GTAC 3 cut(s) 18, 218, 338
RsaNI GTAC 3 cut(s) 17, 217, 337
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 4 cut(s) 67, 138, 159, 244
Sau96I GGNCC 1 cut(s) 267
ScrFI CCNGG 2 cut(s) 167, 265
SduI GDGCHC 1 cut(s) 84
SgrAI CRCCGGYG 1 cut(s) 110
SmlI CTYRAG 2 cut(s) 62, 71
SmoI CTYRAG 2 cut(s) 62, 71
SsiI CCGC 4 cut(s) 77, 94, 114, 274
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 165, 263
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 1 cut(s) 158
TaqII GACCGA 1 cut(s) 326
TatI WGTACW 2 cut(s) 216, 336
TfiI GAWTC 1 cut(s) 9
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TseFI GTSAC 2 cut(s) 106, 183
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 2 cut(s) 106, 183
TspGWI ACGGA 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.