MD01G1119600.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002234
Physical Location & Seq
Reverse (-)
23339936 .. 23341188
1253 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1119600.v1.1.491

Sequence Viewer

Length: 372 bp
ATGGCGAGAATCAAGGTACATGAGCTGAGGCACAAGACAAAGCCTGACCTTTTGGCCCAGCTCAAGGATCTCAAGGCGGAGCTCGCCCTCCTCCGCGTCGCCAAGGTCACCGGCGGAGCGCCCAACAAGCTCTCCAAGATCAAAGTTGTGAGGCTGTCGATTGCCCAGGTGTTGACGGTGATGTCACAGAAGCAGAAGGCTGCTCTCAGAGAAGTGTACAAGAAGAAGAAGCTGTTGCCTCTTGATCTCCGTCCCAAGAAGACCAGGGCCATCCGCCGGAGGCTCACCAAGCACCAGGCATCTTTGAAGACCGAGAGGGAGAAGAAGAAAGAGATGTACTATCCTTTGAGGAAGTATGCCATTAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L29

Protein Analysis

124

Amino Acids

14.29

Weight (kDa)

10.93

Isoelectric Point (pI)

40.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 8 - 64 5.1e-17 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
AccII CGCG 1 cut(s) 96
AciI CCGC 4 cut(s) 77, 94, 114, 274
AclWI GGATC 1 cut(s) 75
AfaI GTAC 3 cut(s) 18, 218, 338
AfiI CCNNNNNNNGG 2 cut(s) 64, 276
AgsI TTSAA 1 cut(s) 307
AjnI CCWGG 3 cut(s) 165, 263, 294
AluBI AGCT 5 cut(s) 25, 61, 82, 130, 232
AluI AGCT 5 cut(s) 25, 61, 82, 130, 232
Alw21I GWGCWC 1 cut(s) 84
AlwI GGATC 1 cut(s) 75
AoxI GGCC 2 cut(s) 54, 267
ApeKI GCWGC 1 cut(s) 200
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 2 cut(s) 55, 267
AsuHPI GGTGA 3 cut(s) 100, 190, 277
BanII GRGCYC 1 cut(s) 84
BbsI GAAGAC 2 cut(s) 266, 314
Bbv12I GWGCWC 1 cut(s) 84
BbvCI CCTCAGC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 187
BccI CCATC 1 cut(s) 278
BciT130I CCWGG 3 cut(s) 167, 265, 296
BfoI RGCGCY 1 cut(s) 122
BglI GCCNNNNNGGC 1 cut(s) 365
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 3 cut(s) 167, 265, 296
BmgT120I GGNCC 2 cut(s) 55, 267
BmrFI CCNGG 3 cut(s) 167, 265, 296
BmsI GCATC 1 cut(s) 308
BpiI GAAGAC 2 cut(s) 266, 314
Bpu10I CCTNAGC 1 cut(s) 26
BpuEI CTTGAG 2 cut(s) 47, 56
BsaJI CCNNGG 3 cut(s) 102, 165, 264
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 276
Bse118I RCCGGY 1 cut(s) 110
BseBI CCWGG 3 cut(s) 167, 265, 296
BseDI CCNNGG 3 cut(s) 102, 165, 264
BseGI GGATG 1 cut(s) 270
BseLI CCNNNNNNNGG 2 cut(s) 64, 276
BseMII CTCAG 2 cut(s) 17, 220
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 187
BseYI CCCAGC 1 cut(s) 57
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 56, 269
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 2 cut(s) 111, 277
BslFI GGGAC 1 cut(s) 237
BslI CCNNNNNNNGG 2 cut(s) 64, 276
BsmFI GGGAC 1 cut(s) 237
BsnI GGCC 2 cut(s) 56, 269
Bsp1286I GDGCHC 1 cut(s) 84
Bsp1407I TGTACA 1 cut(s) 216
Bsp143I GATC 3 cut(s) 67, 138, 244
BspACI CCGC 4 cut(s) 77, 94, 114, 274
BspANI GGCC 2 cut(s) 56, 269
BspCNI CTCAG 2 cut(s) 18, 219
BspFNI CGCG 1 cut(s) 96
BspPI GGATC 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 110
BsrGI TGTACA 1 cut(s) 216
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 3 cut(s) 102, 165, 264
BssMI GATC 3 cut(s) 67, 138, 244
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 3 cut(s) 167, 265, 296
Bst4CI ACNGT 1 cut(s) 178
BstAUI TGTACA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 3 cut(s) 26, 206, 369
BstEII GGTNACC 1 cut(s) 106
BstF5I GGATG 1 cut(s) 270
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 3 cut(s) 70, 141, 247
BstMBI GATC 3 cut(s) 67, 138, 244
BstMWI GCNNNNNNNGC 4 cut(s) 83, 127, 289, 365
BstNI CCWGG 3 cut(s) 167, 265, 296
BstPI GGTNACC 1 cut(s) 106
BstSCI CCNGG 3 cut(s) 165, 263, 294
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 187
BstV2I GAAGAC 2 cut(s) 266, 314
BstX2I RGATCY 1 cut(s) 67
BstYI RGATCY 1 cut(s) 67
BsuRI GGCC 2 cut(s) 56, 269
BtsCI GGATG 1 cut(s) 270
Cac8I GCNNGC 1 cut(s) 84
CfoI GCGC 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 55, 267
CseI GACGC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 17, 217, 337
CviAII CATG 1 cut(s) 20
CviQI GTAC 3 cut(s) 17, 217, 337
DdeI CTNAG 3 cut(s) 26, 206, 369
DpnI GATC 3 cut(s) 69, 140, 246
DpnII GATC 3 cut(s) 67, 138, 244
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
EciI GGCGGA 3 cut(s) 92, 129, 263
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
Eco91I GGTNACC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 82
EcoO65I GGTNACC 1 cut(s) 106
EcoRII CCWGG 3 cut(s) 165, 263, 294
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 1 cut(s) 23
FaiI YATR 2 cut(s) 21, 357
FaqI GGGAC 1 cut(s) 237
FatI CATG 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 257
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 1 cut(s) 201
GlaI GCGC 1 cut(s) 120
GluI GCNGC 1 cut(s) 201
GsaI CCCAGC 1 cut(s) 61
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 2 cut(s) 56, 269
HapII CCGG 2 cut(s) 111, 277
HgaI GACGC 1 cut(s) 85
HhaI GCGC 1 cut(s) 121
Hin1II CATG 1 cut(s) 23
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HinfI GANTC 1 cut(s) 9
HpaII CCGG 2 cut(s) 111, 277
HphI GGTGA 3 cut(s) 100, 190, 277
Hpy166II GTNNAC 2 cut(s) 174, 217
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 1 cut(s) 242
Hpy8I GTNNAC 2 cut(s) 174, 217
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 127, 289, 365
HpyF3I CTNAG 3 cut(s) 26, 206, 369
Hsp92II CATG 1 cut(s) 23
HspAI GCGC 1 cut(s) 119
Kzo9I GATC 3 cut(s) 67, 138, 244
LmnI GCTCC 2 cut(s) 79, 116
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 1 cut(s) 308
MaeIII GTNAC 2 cut(s) 106, 183
MalI GATC 3 cut(s) 69, 140, 246
MboI GATC 3 cut(s) 67, 138, 244
MboII GAAGA 6 cut(s) 235, 238, 271, 319, 334, 337
MflI RGATCY 1 cut(s) 67
MhlI GDGCHC 1 cut(s) 84
MnlI CCTC 8 cut(s) 21, 98, 101, 144, 249, 273, 309, 342
MseI TTAA 1 cut(s) 363
MspI CCGG 2 cut(s) 111, 277
MspR9I CCNGG 3 cut(s) 167, 265, 296
MvaI CCWGG 3 cut(s) 167, 265, 296
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 4 cut(s) 83, 127, 289, 365
NdeII GATC 3 cut(s) 67, 138, 244
NlaIII CATG 1 cut(s) 23
NmuCI GTSAC 2 cut(s) 106, 183
PfeI GAWTC 1 cut(s) 9
PkrI GCNGC 1 cut(s) 202
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 3 cut(s) 165, 263, 294
PspEI GGTNACC 1 cut(s) 106
PspFI CCCAGC 1 cut(s) 57
PspGI CCWGG 3 cut(s) 165, 263, 294
PspPI GGNCC 2 cut(s) 55, 267
PsuI RGATCY 1 cut(s) 67
RsaI GTAC 3 cut(s) 18, 218, 338
RsaNI GTAC 3 cut(s) 17, 217, 337
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 3 cut(s) 67, 138, 244
Sau96I GGNCC 2 cut(s) 55, 267
ScrFI CCNGG 3 cut(s) 167, 265, 296
SduI GDGCHC 1 cut(s) 84
SetI ASST 9 cut(s) 18, 27, 51, 63, 84, 108, 132, 171, 234
SfaNI GCATC 1 cut(s) 308
SgrAI CRCCGGYG 1 cut(s) 110
SmlI CTYRAG 2 cut(s) 62, 71
SmoI CTYRAG 2 cut(s) 62, 71
SsiI CCGC 4 cut(s) 77, 94, 114, 274
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 3 cut(s) 165, 263, 294
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 1 cut(s) 158
TaqII GACCGA 1 cut(s) 326
TatI WGTACW 2 cut(s) 216, 336
TfiI GAWTC 1 cut(s) 9
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TseFI GTSAC 2 cut(s) 106, 183
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 2 cut(s) 106, 183
TspGWI ACGGA 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.