Rmu_sc0005277.1_g000021

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005277.1
OG0002234
Physical Location & Seq
Reverse (-)
81784 .. 83224
1441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005277.1_g000021.1.cds

Sequence Viewer

Length: 372 bp
atggcgaggatcaaggtccacgagctcaggaacaagtccaaggcagatcttttgggtcagctcaaggatctgaaggcggagctcgctcttctccgagtcgccaaggtcactggtggagcccccaacaagctctccaaaatcaaggtggtgaggctttcaatagctcaggtgctgacagtgatttcccagaagcaaaaggcggctcttagggaagtttacaagaacaagaagcttcttcctcttgatttgcgtccaaagaagaccagggctatcaggagacgtctgactaagcaccaggcatccttaaagacagagagggagaagaagagagaaatgtacttcccactaaggaagtacgcaatcaaggcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L29

Protein Analysis

123

Amino Acids

14.19

Weight (kDa)

11.16

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 283
AciI CCGC 2 cut(s) 77, 200
AclWI GGATC 2 cut(s) 17, 75
AcuI CTGAAG 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 280
AfaI GTAC 2 cut(s) 338, 356
AgsI TTSAA 1 cut(s) 159
AjnI CCWGG 2 cut(s) 263, 294
AluBI AGCT 6 cut(s) 25, 61, 82, 130, 164, 232
AluI AGCT 6 cut(s) 25, 61, 82, 130, 164, 232
Alw21I GWGCWC 2 cut(s) 27, 84
Alw26I GTCTC 1 cut(s) 271
AlwI GGATC 2 cut(s) 17, 75
AlwNI CAGNNNCTG 1 cut(s) 172
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 3 cut(s) 27, 84, 121
BauI CACGAG 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 266
Bbv12I GWGCWC 2 cut(s) 27, 84
BciT130I CCWGG 2 cut(s) 265, 296
BcoDI GTCTC 1 cut(s) 271
BglII AGATCT 1 cut(s) 46
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 2 cut(s) 265, 296
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 2 cut(s) 265, 296
BmsI GCATC 1 cut(s) 308
BpiI GAAGAC 1 cut(s) 266
Bpu10I CCTNAGC 2 cut(s) 26, 165
BpuEI CTTGAG 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 280
BsaJI CCNNGG 3 cut(s) 39, 102, 264
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bse1I ACTGG 1 cut(s) 115
BseBI CCWGG 2 cut(s) 265, 296
BseDI CCNNGG 3 cut(s) 39, 102, 264
BseGI GGATG 1 cut(s) 299
BseMII CTCAG 2 cut(s) 40, 179
BseNI ACTGG 1 cut(s) 115
BsiHKAI GWGCWC 2 cut(s) 27, 84
BsmAI GTCTC 1 cut(s) 271
BsmBI CGTCTC 1 cut(s) 271
Bsp1286I GDGCHC 3 cut(s) 27, 84, 121
Bsp143I GATC 3 cut(s) 9, 46, 67
BspACI CCGC 2 cut(s) 77, 200
BspCNI CTCAG 2 cut(s) 39, 178
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 2 cut(s) 17, 75
BspQI GCTCTTC 1 cut(s) 93
BsrI ACTGG 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 39, 102, 264
BssMI GATC 3 cut(s) 9, 46, 67
BssNI GRCGYC 1 cut(s) 280
BssSI CACGAG 1 cut(s) 20
BssT1I CCWWGG 2 cut(s) 39, 102
Bst2BI CACGAG 1 cut(s) 20
Bst2UI CCWGG 2 cut(s) 265, 296
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 2 cut(s) 93, 320
BstACI GRCGYC 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 5 cut(s) 26, 165, 206, 288, 347
BstF5I GGATG 1 cut(s) 299
BstKTI GATC 3 cut(s) 12, 49, 70
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 3 cut(s) 9, 46, 67
BstMWI GCNNNNNNNGC 2 cut(s) 83, 365
BstNI CCWGG 2 cut(s) 265, 296
BstSCI CCNGG 2 cut(s) 263, 294
BstV2I GAAGAC 1 cut(s) 266
BstX2I RGATCY 2 cut(s) 46, 67
BstYI RGATCY 2 cut(s) 46, 67
BtsCI GGATG 1 cut(s) 299
BtsIMutI CAGTG 2 cut(s) 108, 183
Cac8I GCNNGC 1 cut(s) 84
CaiI CAGNNNCTG 1 cut(s) 172
Cfr13I GGNCC 1 cut(s) 16
CseI GACGC 1 cut(s) 239
Csp6I GTAC 2 cut(s) 337, 355
CviQI GTAC 2 cut(s) 337, 355
DdeI CTNAG 5 cut(s) 26, 165, 206, 288, 347
DpnI GATC 3 cut(s) 11, 48, 69
DpnII GATC 3 cut(s) 9, 46, 67
Eam1104I CTCTTC 2 cut(s) 93, 320
EarI CTCTTC 2 cut(s) 93, 320
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 2 cut(s) 25, 82
Eco130I CCWWGG 2 cut(s) 39, 102
Eco24I GRGCYC 3 cut(s) 27, 84, 121
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 2 cut(s) 25, 82
Eco57I CTGAAG 1 cut(s) 92
EcoICRI GAGCTC 2 cut(s) 25, 82
EcoRII CCWGG 2 cut(s) 263, 294
EcoT14I CCWWGG 2 cut(s) 39, 102
EcoT38I GRGCYC 3 cut(s) 27, 84, 121
ErhI CCWWGG 2 cut(s) 39, 102
Esp3I CGTCTC 1 cut(s) 271
FaiI YATR 1 cut(s) 370
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 1 cut(s) 286
FriOI GRGCYC 3 cut(s) 27, 84, 121
Fsp4HI GCNGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 201
HgaI GACGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 280
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 1 cut(s) 96
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 2 cut(s) 19, 217
Hpy188I TCNGA 3 cut(s) 72, 95, 285
Hpy188III TCNNGA 3 cut(s) 28, 242, 274
Hpy8I GTNNAC 2 cut(s) 19, 217
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 365
HpyF3I CTNAG 5 cut(s) 26, 165, 206, 288, 347
HpySE526I ACGT 1 cut(s) 280
Hsp92I GRCGYC 1 cut(s) 280
Kzo9I GATC 3 cut(s) 9, 46, 67
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 2 cut(s) 79, 116
LpnPI CCDG 9 cut(s) 13, 96, 152, 200, 250, 259, 277, 281, 308
LweI GCATC 1 cut(s) 308
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 1 cut(s) 106
MalI GATC 3 cut(s) 11, 48, 69
MboI GATC 3 cut(s) 9, 46, 67
MboII GAAGA 5 cut(s) 80, 227, 271, 334, 337
MflI RGATCY 2 cut(s) 46, 67
MhlI GDGCHC 3 cut(s) 27, 84, 121
MlyI GAGTC 1 cut(s) 105
MnlI CCTC 3 cut(s) 144, 249, 309
MseI TTAA 1 cut(s) 305
MspR9I CCNGG 2 cut(s) 265, 296
MvaI CCWGG 2 cut(s) 265, 296
MwoI GCNNNNNNNGC 2 cut(s) 83, 365
NdeII GATC 3 cut(s) 9, 46, 67
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 93
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Psp124BI GAGCTC 2 cut(s) 27, 84
Psp6I CCWGG 2 cut(s) 263, 294
PspGI CCWGG 2 cut(s) 263, 294
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 16
PstNI CAGNNNCTG 1 cut(s) 172
PsuI RGATCY 2 cut(s) 46, 67
RsaI GTAC 2 cut(s) 338, 356
RsaNI GTAC 2 cut(s) 337, 355
SacI GAGCTC 2 cut(s) 27, 84
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 3 cut(s) 9, 46, 67
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 2 cut(s) 265, 296
SduI GDGCHC 3 cut(s) 27, 84, 121
SfaNI GCATC 1 cut(s) 308
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SsiI CCGC 2 cut(s) 77, 200
SstI GAGCTC 2 cut(s) 27, 84
StyD4I CCNGG 2 cut(s) 263, 294
StyI CCWWGG 2 cut(s) 39, 102
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 1 cut(s) 283
TatI WGTACW 1 cut(s) 336
TauI GCSGC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 2 cut(s) 115, 183
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspRI CASTG 2 cut(s) 115, 183
VpaK11BI GGWCC 1 cut(s) 16
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
ZraI GACGTC 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.