Rroxscaffold_4G00279280

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002234
Physical Location & Seq
Reverse (-)
2112414 .. 2112789
376 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00279280.1

Sequence Viewer

Length: 291 bp
ATGGCGAGGATCAAGGTCCACGAGCTCAGGAACAAAACCAAGGCAGATCTTTTGGGTCAGCTCAAGGATCTGAAGGCGGAGCGTGCTCTTCTCCGAGTCGCCAAGGTCACTGGTAGAGCCCTCAACAAGCTCTCCAAAATCAAGGTTGTGAGGCTTTCAATAGCTCAGGTGTTGACAAAGACCGGGGCTATCAGGAGGCGTCTGACTAAGCACCAGGAATCTGTAAAGACTGAGAAGGAGAAGAAGAGAGAGATGTACTTCCCACTAAGAAAGTACGCAATCAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L29

Protein Analysis

96

Amino Acids

11.12

Weight (kDa)

11.13

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 8 - 59 8.2e-14 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 77
AclWI GGATC 2 cut(s) 17, 75
AcuI CTGAAG 1 cut(s) 92
AcyI GRCGYC 1 cut(s) 199
AfaI GTAC 2 cut(s) 257, 275
AgsI TTSAA 1 cut(s) 159
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 4 cut(s) 25, 61, 130, 164
AluI AGCT 4 cut(s) 25, 61, 130, 164
Alw21I GWGCWC 2 cut(s) 27, 88
AlwI GGATC 2 cut(s) 17, 75
AspS9I GGNCC 1 cut(s) 16
AsuC2I CCSGG 1 cut(s) 184
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 2 cut(s) 27, 121
BauI CACGAG 1 cut(s) 20
Bbv12I GWGCWC 2 cut(s) 27, 88
BciT130I CCWGG 1 cut(s) 215
BcnI CCSGG 1 cut(s) 184
BglII AGATCT 1 cut(s) 46
Bme1390I CCNGG 2 cut(s) 184, 215
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmrFI CCNGG 2 cut(s) 184, 215
Bpu10I CCTNAGC 2 cut(s) 26, 165
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 1 cut(s) 184
BsaHI GRCGYC 1 cut(s) 199
BsaJI CCNNGG 3 cut(s) 39, 102, 183
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bse1I ACTGG 1 cut(s) 115
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 3 cut(s) 39, 102, 183
BseMII CTCAG 3 cut(s) 40, 179, 222
BseNI ACTGG 1 cut(s) 115
BsiHKAI GWGCWC 2 cut(s) 27, 88
BsiSI CCGG 1 cut(s) 183
Bsp1286I GDGCHC 3 cut(s) 27, 88, 121
Bsp143I GATC 3 cut(s) 9, 46, 67
BspACI CCGC 1 cut(s) 77
BspCNI CTCAG 3 cut(s) 39, 178, 223
BspPI GGATC 2 cut(s) 17, 75
BspQI GCTCTTC 1 cut(s) 93
BsrI ACTGG 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 39, 102, 183
BssMI GATC 3 cut(s) 9, 46, 67
BssNI GRCGYC 1 cut(s) 199
BssSI CACGAG 1 cut(s) 20
BssT1I CCWWGG 2 cut(s) 39, 102
Bst2BI CACGAG 1 cut(s) 20
Bst2UI CCWGG 1 cut(s) 215
Bst6I CTCTTC 2 cut(s) 93, 239
BstACI GRCGYC 1 cut(s) 199
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 5 cut(s) 26, 165, 207, 231, 266
BstKTI GATC 3 cut(s) 12, 49, 70
BstMBI GATC 3 cut(s) 9, 46, 67
BstMWI GCNNNNNNNGC 2 cut(s) 83, 284
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 2 cut(s) 182, 213
BstX2I RGATCY 2 cut(s) 46, 67
BstYI RGATCY 2 cut(s) 46, 67
BtsIMutI CAGTG 1 cut(s) 108
Cac8I GCNNGC 1 cut(s) 84
Cfr13I GGNCC 1 cut(s) 16
CseI GACGC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 256, 274
CviAII CATG 1 cut(s) 288
CviJI RGCY 7 cut(s) 25, 61, 119, 130, 154, 164, 188
CviKI_1 RGCY 7 cut(s) 25, 61, 119, 130, 154, 164, 188
CviQI GTAC 2 cut(s) 256, 274
DdeI CTNAG 5 cut(s) 26, 165, 207, 231, 266
DpnI GATC 3 cut(s) 11, 48, 69
DpnII GATC 3 cut(s) 9, 46, 67
Eam1104I CTCTTC 2 cut(s) 93, 239
EarI CTCTTC 2 cut(s) 93, 239
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 39, 102
Eco24I GRGCYC 2 cut(s) 27, 121
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 2 cut(s) 39, 102
EcoT38I GRGCYC 2 cut(s) 27, 121
ErhI CCWWGG 2 cut(s) 39, 102
FaeI CATG 1 cut(s) 291
FaiI YATR 1 cut(s) 289
FatI CATG 1 cut(s) 287
FriOI GRGCYC 2 cut(s) 27, 121
HapII CCGG 1 cut(s) 183
HgaI GACGC 1 cut(s) 188
Hin1I GRCGYC 1 cut(s) 199
Hin1II CATG 1 cut(s) 291
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HinfI GANTC 2 cut(s) 96, 218
HpaII CCGG 1 cut(s) 183
Hpy166II GTNNAC 2 cut(s) 19, 174
Hpy188I TCNGA 3 cut(s) 72, 95, 204
Hpy188III TCNNGA 2 cut(s) 28, 193
Hpy8I GTNNAC 2 cut(s) 19, 174
HpyAV CCTTC 2 cut(s) 67, 229
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 284
HpyF3I CTNAG 5 cut(s) 26, 165, 207, 231, 266
Hsp92I GRCGYC 1 cut(s) 199
Hsp92II CATG 1 cut(s) 291
Kzo9I GATC 3 cut(s) 9, 46, 67
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 7 cut(s) 13, 96, 152, 178, 196, 200, 227
MaeIII GTNAC 1 cut(s) 106
MalI GATC 3 cut(s) 11, 48, 69
MboI GATC 3 cut(s) 9, 46, 67
MboII GAAGA 3 cut(s) 80, 253, 256
MflI RGATCY 2 cut(s) 46, 67
MhlI GDGCHC 3 cut(s) 27, 88, 121
MlyI GAGTC 1 cut(s) 105
MnlI CCTC 3 cut(s) 131, 144, 189
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 184, 215
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 2 cut(s) 83, 284
NciI CCSGG 1 cut(s) 184
NdeII GATC 3 cut(s) 9, 46, 67
NlaIII CATG 1 cut(s) 291
NmuCI GTSAC 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 218
PleI GAGTC 1 cut(s) 104
PpsI GAGTC 1 cut(s) 104
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspPI GGNCC 1 cut(s) 16
PsuI RGATCY 2 cut(s) 46, 67
RsaI GTAC 2 cut(s) 257, 275
RsaNI GTAC 2 cut(s) 256, 274
SacI GAGCTC 1 cut(s) 27
SapI GCTCTTC 1 cut(s) 93
Sau3AI GATC 3 cut(s) 9, 46, 67
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 2 cut(s) 184, 215
SduI GDGCHC 3 cut(s) 27, 88, 121
SetI ASST 8 cut(s) 18, 27, 63, 108, 132, 147, 166, 171
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
SsiI CCGC 1 cut(s) 77
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 182, 213
StyI CCWWGG 2 cut(s) 39, 102
TatI WGTACW 1 cut(s) 255
TfiI GAWTC 1 cut(s) 218
TscAI CASTG 1 cut(s) 115
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspRI CASTG 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.