MD11G1116100.v1.1

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002234
Physical Location & Seq
Forward (+)
10476458 .. 10478917
2460 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1116100.v1.1.491

Sequence Viewer

Length: 372 bp
ATGGCGAGGATTAAGGTCCATGAACTTAGGCACAAAACCAAGGCAGATCTTTTGGCTCAGCTGAAGGATCTGAAGGCAGAGCTCGCTCTTCTCCGGGTCGCCAAGGTCACCGGTGGAGCCCCCAACAAACTCTCCAAAATCAAGGTGGTGAGGCTTTCGATCGCTCAGGTGTTGACAGTGATGTCCCAGAAGCAAAAGGCGGCTCTTAGAGAAGTCTACAGGAACAAGAAGCTCCTTCCCCTCGATTTGCGTCCCAAGAAGACCAGGGCCATCAGGAGGCGTCTCACCAAGCACCAGGCATCTTTGAAGACAGAAAGGGAGAAGAAAAAGGAGATGTACTACCCATCGAGGAAGTACGCAATCAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L29

Protein Analysis

124

Amino Acids

14.25

Weight (kDa)

11.02

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L29 PF00831 8 - 64 2.3e-17 Ribosomal L29 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 181
AccI GTMKAC 1 cut(s) 216
AciI CCGC 1 cut(s) 200
AclWI GGATC 1 cut(s) 75
AcuI CTGAAG 2 cut(s) 83, 92
AcyI GRCGYC 1 cut(s) 280
AfaI GTAC 2 cut(s) 338, 356
AfiI CCNNNNNNNGG 1 cut(s) 276
AgeI ACCGGT 1 cut(s) 110
AgsI TTSAA 1 cut(s) 307
AjnI CCWGG 2 cut(s) 263, 294
AluBI AGCT 3 cut(s) 61, 82, 232
AluI AGCT 3 cut(s) 61, 82, 232
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 1 cut(s) 287
AlwI GGATC 1 cut(s) 75
AoxI GGCC 1 cut(s) 267
AsiGI ACCGGT 1 cut(s) 110
AspS9I GGNCC 2 cut(s) 16, 267
AsuC2I CCSGG 1 cut(s) 95
AsuHPI GGTGA 3 cut(s) 100, 160, 277
AvaII GGWCC 1 cut(s) 16
BanII GRGCYC 2 cut(s) 84, 121
BbsI GAAGAC 2 cut(s) 266, 314
Bbv12I GWGCWC 1 cut(s) 84
BccI CCATC 2 cut(s) 278, 352
BciT130I CCWGG 2 cut(s) 265, 296
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 1 cut(s) 287
BfmI CTRYAG 1 cut(s) 217
BglII AGATCT 1 cut(s) 46
BisI GCNGC 1 cut(s) 201
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 3 cut(s) 95, 265, 296
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 2 cut(s) 16, 267
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 3 cut(s) 95, 265, 296
BmsI GCATC 1 cut(s) 308
BpiI GAAGAC 2 cut(s) 266, 314
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 57
BpuMI CCSGG 1 cut(s) 95
BsaHI GRCGYC 1 cut(s) 280
BsaJI CCNNGG 3 cut(s) 39, 102, 264
BsaWI WCCGGW 1 cut(s) 110
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse118I RCCGGY 1 cut(s) 110
BseBI CCWGG 2 cut(s) 265, 296
BseDI CCNNGG 3 cut(s) 39, 102, 264
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMII CTCAG 2 cut(s) 71, 179
Bsh1285I CGRYCG 1 cut(s) 162
BshFI GGCC 1 cut(s) 269
BshTI ACCGGT 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 84
BsiSI CCGG 2 cut(s) 94, 111
BslFI GGGAC 2 cut(s) 169, 237
BslI CCNNNNNNNGG 1 cut(s) 276
BsmAI GTCTC 1 cut(s) 287
BsmBI CGTCTC 1 cut(s) 287
BsmFI GGGAC 2 cut(s) 169, 237
BsnI GGCC 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 84, 121
Bsp143I GATC 3 cut(s) 46, 67, 159
Bsp1720I GCTNAGC 1 cut(s) 57
BspACI CCGC 1 cut(s) 200
BspANI GGCC 1 cut(s) 269
BspCNI CTCAG 2 cut(s) 70, 178
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 1 cut(s) 75
BspQI GCTCTTC 1 cut(s) 93
BsrFI RCCGGY 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 3 cut(s) 39, 102, 264
BssMI GATC 3 cut(s) 46, 67, 159
BssNI GRCGYC 1 cut(s) 280
BssT1I CCWWGG 2 cut(s) 39, 102
Bst2UI CCWGG 2 cut(s) 265, 296
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 1 cut(s) 93
BstACI GRCGYC 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 4 cut(s) 26, 57, 165, 206
BstEII GGTNACC 1 cut(s) 106
BstKTI GATC 3 cut(s) 49, 70, 162
BstMAI GTCTC 1 cut(s) 287
BstMBI GATC 3 cut(s) 46, 67, 159
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 2 cut(s) 83, 365
BstNI CCWGG 2 cut(s) 265, 296
BstPI GGTNACC 1 cut(s) 106
BstSCI CCNGG 3 cut(s) 93, 263, 294
BstSFI CTRYAG 1 cut(s) 217
BstV2I GAAGAC 2 cut(s) 266, 314
BstX2I RGATCY 2 cut(s) 46, 67
BstYI RGATCY 2 cut(s) 46, 67
BsuRI GGCC 1 cut(s) 269
BtsIMutI CAGTG 1 cut(s) 183
Cac8I GCNNGC 1 cut(s) 84
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 16, 267
CseI GACGC 2 cut(s) 239, 269
Csp6I GTAC 2 cut(s) 337, 355
CspAI ACCGGT 1 cut(s) 110
CviAII CATG 2 cut(s) 20, 369
CviJI RGCY 8 cut(s) 56, 61, 82, 119, 154, 203, 232, 269
CviKI_1 RGCY 8 cut(s) 56, 61, 82, 119, 154, 203, 232, 269
CviQI GTAC 2 cut(s) 337, 355
DdeI CTNAG 4 cut(s) 26, 57, 165, 206
DpnI GATC 3 cut(s) 48, 69, 161
DpnII GATC 3 cut(s) 46, 67, 159
DrdI GACNNNNNNGTC 1 cut(s) 181
DseDI GACNNNNNNGTC 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 93
EarI CTCTTC 1 cut(s) 93
Ecl136II GAGCTC 1 cut(s) 82
Eco130I CCWWGG 2 cut(s) 39, 102
Eco24I GRGCYC 2 cut(s) 84, 121
Eco47I GGWCC 1 cut(s) 16
Eco53kI GAGCTC 1 cut(s) 82
Eco57I CTGAAG 2 cut(s) 83, 92
Eco91I GGTNACC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 82
EcoO65I GGTNACC 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 263, 294
EcoT14I CCWWGG 2 cut(s) 39, 102
EcoT38I GRGCYC 2 cut(s) 84, 121
ErhI CCWWGG 2 cut(s) 39, 102
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 2 cut(s) 23, 372
FaiI YATR 2 cut(s) 21, 370
FaqI GGGAC 2 cut(s) 169, 237
FatI CATG 2 cut(s) 19, 368
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 1 cut(s) 201
FriOI GRGCYC 2 cut(s) 84, 121
Fsp4HI GCNGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 1 cut(s) 269
HapII CCGG 2 cut(s) 94, 111
HgaI GACGC 2 cut(s) 239, 269
Hin1I GRCGYC 1 cut(s) 280
Hin1II CATG 2 cut(s) 23, 372
HincII GTYRAC 1 cut(s) 174
HindII GTYRAC 1 cut(s) 174
HpaII CCGG 2 cut(s) 94, 111
HphI GGTGA 3 cut(s) 100, 160, 277
Hpy166II GTNNAC 2 cut(s) 174, 217
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 1 cut(s) 274
Hpy8I GTNNAC 2 cut(s) 174, 217
HpyAV CCTTC 3 cut(s) 58, 67, 245
HpyCH4III ACNGT 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 365
HpyF3I CTNAG 4 cut(s) 26, 57, 165, 206
Hsp92I GRCGYC 1 cut(s) 280
Hsp92II CATG 2 cut(s) 23, 372
Kzo9I GATC 3 cut(s) 46, 67, 159
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 2 cut(s) 116, 237
LweI GCATC 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 106
MalI GATC 3 cut(s) 48, 69, 161
MboI GATC 3 cut(s) 46, 67, 159
MboII GAAGA 4 cut(s) 80, 271, 319, 334
MflI RGATCY 2 cut(s) 46, 67
MhlI GDGCHC 2 cut(s) 84, 121
MnlI CCTC 4 cut(s) 144, 251, 270, 342
MseI TTAA 1 cut(s) 12
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 2 cut(s) 94, 111
MspR9I CCNGG 3 cut(s) 95, 265, 296
MvaI CCWGG 2 cut(s) 265, 296
MwoI GCNNNNNNNGC 2 cut(s) 83, 365
NciI CCSGG 1 cut(s) 95
NdeII GATC 3 cut(s) 46, 67, 159
NlaIII CATG 2 cut(s) 23, 372
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 93
PinAI ACCGGT 1 cut(s) 110
PkrI GCNGC 1 cut(s) 202
Ple19I CGATCG 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 84
Psp6I CCWGG 2 cut(s) 263, 294
PspEI GGTNACC 1 cut(s) 106
PspGI CCWGG 2 cut(s) 263, 294
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 2 cut(s) 16, 267
PsuI RGATCY 2 cut(s) 46, 67
PvuI CGATCG 1 cut(s) 162
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 2 cut(s) 338, 356
RsaNI GTAC 2 cut(s) 337, 355
SacI GAGCTC 1 cut(s) 84
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 3 cut(s) 46, 67, 159
Sau96I GGNCC 2 cut(s) 16, 267
ScrFI CCNGG 3 cut(s) 95, 265, 296
SduI GDGCHC 2 cut(s) 84, 121
SetI ASST 7 cut(s) 18, 63, 84, 108, 147, 171, 234
SfaNI GCATC 1 cut(s) 308
SfcI CTRYAG 1 cut(s) 217
SgrAI CRCCGGYG 1 cut(s) 110
SinI GGWCC 1 cut(s) 16
SsiI CCGC 1 cut(s) 200
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 3 cut(s) 93, 263, 294
StyI CCWWGG 2 cut(s) 39, 102
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 3 cut(s) 158, 243, 347
TatI WGTACW 1 cut(s) 336
TauI GCSGC 1 cut(s) 203
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TscAI CASTG 1 cut(s) 183
TseFI GTSAC 1 cut(s) 106
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 1 cut(s) 36
TspRI CASTG 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 16
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.