FvH4_3g40460

Non-specific lipid-transfer protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003691
Physical Location & Seq
Forward (+)
34155558 .. 34156554
997 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40460.t1

Sequence Viewer

Length: 360 bp
ATGGCCAGATTTCAAGTTGGTGTTGCGTTCATGGTTTTACTAATATCCATGGCGATGACCACTCCTTATGTCACTAAAGCAACCATAACTTGCGGTGAGGTGACGACCTTACTCACTCCCTGCATTCCATTTGGAGTGTTGGGAGGGATCGTGCTGCCGGATTGTTGTGCCGGGATCAAGGGGCTCAATGCTGCACAAAATACGACAGAGGATCGAAGAACGGCTTGTACTTGCATTCAAGAAGGAGCAGCTAGGATTCCTGGGATTGATTATGATCGTATAAACATGCTGGGCGATCTTTGTGGTTCTCCTTGTCCCTACAAAGTTTATCCATCTACTGATTGCTCTCAGGTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

120

Amino Acids

12.59

Weight (kDa)

5.13

Isoelectric Point (pI)

28.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 31 - 115 2.1e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 93
AclWI GGATC 3 cut(s) 155, 182, 219
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 229
AgsI TTSAA 2 cut(s) 14, 239
AjnI CCWGG 1 cut(s) 259
AluBI AGCT 2 cut(s) 251, 357
AluI AGCT 2 cut(s) 251, 357
AlwI GGATC 3 cut(s) 155, 182, 219
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 154, 191, 248
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 2 cut(s) 107, 112
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 186
BbvI GCAGC 3 cut(s) 141, 178, 260
BccI CCATC 1 cut(s) 340
BceAI ACGGC 1 cut(s) 237
BciT130I CCWGG 1 cut(s) 261
BcnI CCSGG 1 cut(s) 172
BfaI CTAG 1 cut(s) 252
BisI GCNGC 3 cut(s) 155, 192, 249
BlsI GCNGC 3 cut(s) 156, 193, 250
Bme1390I CCNGG 2 cut(s) 172, 261
BmrFI CCNGG 2 cut(s) 172, 261
BpuMI CCSGG 1 cut(s) 172
BsaBI GATNNNNATC 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 48, 260
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bse8I GATNNNNATC 1 cut(s) 273
BseBI CCWGG 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 48, 260
BseJI GATNNNNATC 1 cut(s) 273
BseXI GCAGC 3 cut(s) 141, 178, 260
BseYI CCCAGC 1 cut(s) 289
BsgI GTGCAG 1 cut(s) 177
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 2 cut(s) 158, 171
BslFI GGGAC 1 cut(s) 300
BsmFI GGGAC 1 cut(s) 300
BsmI GAATGC 2 cut(s) 123, 234
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 5 cut(s) 147, 174, 211, 274, 295
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 1 cut(s) 93
BspANI GGCC 1 cut(s) 5
BspPI GGATC 3 cut(s) 155, 182, 219
BssECI CCNNGG 2 cut(s) 48, 260
BssMI GATC 5 cut(s) 147, 174, 211, 274, 295
BssT1I CCWWGG 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 261
BstDEI CTNAG 1 cut(s) 348
BstDSI CCRYGG 1 cut(s) 48
BstKTI GATC 5 cut(s) 150, 177, 214, 277, 298
BstMBI GATC 5 cut(s) 147, 174, 211, 274, 295
BstNI CCWGG 1 cut(s) 261
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 2 cut(s) 170, 259
BstV1I GCAGC 3 cut(s) 141, 178, 260
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 48
BtgZI GCGATG 1 cut(s) 68
Csp6I GTAC 1 cut(s) 228
CviAII CATG 3 cut(s) 31, 49, 286
CviJI RGCY 5 cut(s) 5, 184, 224, 251, 357
CviKI_1 RGCY 5 cut(s) 5, 184, 224, 251, 357
CviQI GTAC 1 cut(s) 228
DdeI CTNAG 1 cut(s) 348
DpnI GATC 5 cut(s) 149, 176, 213, 276, 297
DpnII GATC 5 cut(s) 147, 174, 211, 274, 295
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 186
EcoRII CCWGG 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 3 cut(s) 34, 52, 289
FaiI YATR 7 cut(s) 32, 50, 69, 86, 273, 281, 287
FalI AAGNNNNNCTT 2 cut(s) 208, 240
FaqI GGGAC 1 cut(s) 300
FatI CATG 3 cut(s) 30, 48, 285
Fnu4HI GCNGC 3 cut(s) 155, 192, 249
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 155, 192, 249
FspBI CTAG 1 cut(s) 252
GluI GCNGC 3 cut(s) 155, 192, 249
GsaI CCCAGC 1 cut(s) 293
HaeIII GGCC 1 cut(s) 5
HapII CCGG 2 cut(s) 158, 171
Hin1II CATG 3 cut(s) 34, 52, 289
HinfI GANTC 1 cut(s) 256
HpaII CCGG 2 cut(s) 158, 171
HphI GGTGA 2 cut(s) 107, 112
Hpy188III TCNNGA 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 236
HpyCH4V TGCA 3 cut(s) 123, 194, 234
HpyF3I CTNAG 1 cut(s) 348
Hsp92II CATG 3 cut(s) 34, 52, 289
Kzo9I GATC 5 cut(s) 147, 174, 211, 274, 295
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 8 cut(s) 19, 133, 171, 184, 246, 273, 275, 335
Lsp1109I GCAGC 3 cut(s) 141, 178, 260
MaeI CTAG 1 cut(s) 252
MaeIII GTNAC 2 cut(s) 70, 100
MalI GATC 5 cut(s) 149, 176, 213, 276, 297
MboI GATC 5 cut(s) 147, 174, 211, 274, 295
MboII GAAGA 1 cut(s) 228
MhlI GDGCHC 1 cut(s) 186
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 3 cut(s) 91, 137, 202
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 53
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 158, 171
MspR9I CCNGG 2 cut(s) 172, 261
Mva1269I GAATGC 2 cut(s) 123, 234
MvaI CCWGG 1 cut(s) 261
NciI CCSGG 1 cut(s) 172
NcoI CCATGG 1 cut(s) 48
NdeII GATC 5 cut(s) 147, 174, 211, 274, 295
NlaIII CATG 3 cut(s) 34, 52, 289
NmuCI GTSAC 2 cut(s) 70, 100
NspI RCATGY 1 cut(s) 289
PctI GAATGC 2 cut(s) 123, 234
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 3 cut(s) 156, 193, 250
Psp6I CCWGG 1 cut(s) 259
PspFI CCCAGC 1 cut(s) 289
PspGI CCWGG 1 cut(s) 259
PsrI GAACNNNNNNTAC 2 cut(s) 211, 243
RsaI GTAC 1 cut(s) 229
RsaNI GTAC 1 cut(s) 228
RseI CAYNNNNRTG 1 cut(s) 53
SatI GCNGC 3 cut(s) 155, 192, 249
Sau3AI GATC 5 cut(s) 147, 174, 211, 274, 295
ScrFI CCNGG 2 cut(s) 172, 261
SduI GDGCHC 1 cut(s) 186
SetI ASST 5 cut(s) 102, 110, 253, 354, 359
SmiMI CAYNNNNRTG 1 cut(s) 53
SsiI CCGC 1 cut(s) 93
SspMI CTAG 1 cut(s) 252
StyD4I CCNGG 2 cut(s) 170, 259
StyI CCWWGG 1 cut(s) 48
TaqI TCGA 1 cut(s) 214
TatI WGTACW 1 cut(s) 227
TfiI GAWTC 1 cut(s) 256
TseFI GTSAC 2 cut(s) 70, 100
TseI GCWGC 3 cut(s) 154, 191, 248
Tsp45I GTSAC 2 cut(s) 70, 100
TspDTI ATGAA 1 cut(s) 19
XceI RCATGY 1 cut(s) 289
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.