Rh5DG511100

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003691
Physical Location & Seq
Forward (+)
79805566 .. 79806026
461 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG511100.1

Sequence Viewer

Length: 363 bp
ATGGCCAGGTTTGCAGGCCATGGGTTGACATTCTTGGTGATACTGGTATCTGTTCTGGTGACGACTCCGTATGTCATTAAGGCAGCTGTAACCTGCGAGCAGGTCGTAAACTATCTGACTCCATGCATCCCTTTTGGAGTGTTTGGAGGGAGCGTACCAGATGAGTGTTGTGCCGGCGTAAAAGGGCTTCATGATGCAGAAGTCACGACAGAGGATCGGAGAACTGCTTGCGGTTGCATTCAGAAAGGGTGTGCAATGATTCCTGGTCTCGACTATGACCGTGTAAACACGCTTGGGGATCTATGTGGTGCTCCTTGTCCTTATAAAGTTTACCCTTCTACTAATTGCTCTCAGGTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

120

Amino Acids

12.68

Weight (kDa)

5.51

Isoelectric Point (pI)

29.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 116 5.1e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 324
Acc36I ACCTGC 2 cut(s) 91, 101
AciI CCGC 1 cut(s) 231
AclWI GGATC 2 cut(s) 222, 306
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 156
AjnI CCWGG 2 cut(s) 5, 262
AluBI AGCT 2 cut(s) 86, 360
AluI AGCT 2 cut(s) 86, 360
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 272
AlwI GGATC 2 cut(s) 222, 306
AoxI GGCC 2 cut(s) 3, 16
ApeKI GCWGC 1 cut(s) 83
AsuHPI GGTGA 2 cut(s) 49, 70
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 171, 203
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 1 cut(s) 95
BciT130I CCWGG 2 cut(s) 7, 264
BcoDI GTCTC 1 cut(s) 272
BfuAI ACCTGC 2 cut(s) 91, 101
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 7, 264
BmrFI CCNGG 2 cut(s) 7, 264
BmsI GCATC 2 cut(s) 135, 184
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 19
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 48
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 2 cut(s) 7, 264
BseDI CCNNGG 1 cut(s) 19
BseGI GGATG 1 cut(s) 126
BseMI GCAATG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 48
BseXI GCAGC 1 cut(s) 95
BshFI GGCC 2 cut(s) 5, 18
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 2 cut(s) 5, 18
Bso31I GGTCTC 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 2 cut(s) 214, 298
Bsp19I CCATGG 1 cut(s) 19
BspACI CCGC 1 cut(s) 231
BspANI GGCC 2 cut(s) 5, 18
BspHI TCATGA 1 cut(s) 190
BspMI ACCTGC 2 cut(s) 91, 101
BspPI GGATC 2 cut(s) 222, 306
BspTNI GGTCTC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 1 cut(s) 48
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 2 cut(s) 214, 298
BssT1I CCWWGG 1 cut(s) 19
Bst2UI CCWGG 2 cut(s) 7, 264
Bst4CI ACNGT 1 cut(s) 281
BstC8I GCNNGC 4 cut(s) 16, 98, 175, 229
BstDEI CTNAG 1 cut(s) 351
BstDSI CCRYGG 1 cut(s) 19
BstF5I GGATG 1 cut(s) 126
BstKTI GATC 2 cut(s) 217, 301
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 2 cut(s) 214, 298
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 7, 264
BstSCI CCNGG 2 cut(s) 5, 262
BstV1I GCAGC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 2 cut(s) 5, 18
BtgI CCRYGG 1 cut(s) 19
BtsCI GGATG 1 cut(s) 126
BveI ACCTGC 2 cut(s) 91, 101
Cac8I GCNNGC 4 cut(s) 16, 98, 175, 229
CciI TCATGA 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 173
Csp6I GTAC 1 cut(s) 155
CviAII CATG 3 cut(s) 20, 123, 191
CviJI RGCY 5 cut(s) 5, 18, 86, 187, 360
CviKI_1 RGCY 5 cut(s) 5, 18, 86, 187, 360
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 1 cut(s) 351
DpnI GATC 2 cut(s) 216, 300
DpnII GATC 2 cut(s) 214, 298
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 19
Eco31I GGTCTC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 5, 262
EcoT14I CCWWGG 1 cut(s) 19
EcoT22I ATGCAT 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 19
FaeI CATG 3 cut(s) 23, 126, 194
FaiI YATR 7 cut(s) 21, 72, 124, 192, 276, 304, 324
FatI CATG 3 cut(s) 19, 122, 190
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 1 cut(s) 84
GluI GCNGC 1 cut(s) 84
HaeIII GGCC 2 cut(s) 5, 18
HapII CCGG 1 cut(s) 174
Hin1II CATG 3 cut(s) 23, 126, 194
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 3 cut(s) 64, 118, 259
HpaII CCGG 1 cut(s) 174
HphI GGTGA 2 cut(s) 49, 70
Hpy166II GTNNAC 4 cut(s) 27, 109, 286, 331
Hpy188I TCNGA 3 cut(s) 117, 219, 243
Hpy188III TCNNGA 3 cut(s) 191, 205, 269
Hpy8I GTNNAC 4 cut(s) 27, 109, 286, 331
HpyAV CCTTC 1 cut(s) 345
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4V TGCA 5 cut(s) 14, 126, 197, 237, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 351
Hsp92II CATG 3 cut(s) 23, 126, 194
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 214, 298
LmnI GCTCC 2 cut(s) 150, 316
Lsp1109I GCAGC 1 cut(s) 95
LweI GCATC 2 cut(s) 135, 184
MaeIII GTNAC 3 cut(s) 58, 88, 202
MalI GATC 2 cut(s) 216, 300
MboI GATC 2 cut(s) 214, 298
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 1 cut(s) 313
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 58, 112
MnlI CCTC 2 cut(s) 140, 205
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 128
MroNI GCCGGC 1 cut(s) 173
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 78
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 7, 264
Mva1269I GAATGC 1 cut(s) 237
MvaI CCWGG 2 cut(s) 7, 264
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 175
NcoI CCATGG 1 cut(s) 19
NdeII GATC 2 cut(s) 214, 298
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 3 cut(s) 23, 126, 194
NmuCI GTSAC 2 cut(s) 58, 202
NsiI ATGCAT 1 cut(s) 128
PagI TCATGA 1 cut(s) 190
PctI GAATGC 1 cut(s) 237
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 2 cut(s) 58, 112
PpsI GAGTC 2 cut(s) 58, 112
PsiI TTATAA 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 5, 262
PspGI CCWGG 2 cut(s) 5, 262
PsuI RGATCY 1 cut(s) 298
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 214, 298
SchI GAGTC 2 cut(s) 58, 112
ScrFI CCNGG 2 cut(s) 7, 264
SduI GDGCHC 1 cut(s) 313
SetI ASST 6 cut(s) 11, 88, 95, 105, 357, 362
SfaNI GCATC 2 cut(s) 135, 184
Sse9I AATT 1 cut(s) 343
SsiI CCGC 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 5, 262
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 1 cut(s) 281
TaqI TCGA 1 cut(s) 270
TasI AATT 1 cut(s) 343
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseFI GTSAC 2 cut(s) 58, 202
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 2 cut(s) 58, 202
TspDTI ATGAA 1 cut(s) 179
TspGWI ACGGA 1 cut(s) 57
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.