Rh5AG478300

Non-specific lipid-transfer protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003691
Physical Location & Seq
Forward (+)
82223154 .. 82224058
905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG478300.1

Sequence Viewer

Length: 363 bp
ATGGCCAGATTTGAAGTCTATGGGTTTGCTTTCATGGTGCTACTGGTAGCCATGGTGGTGACTACTCCTTATGCCACTAATGCAACCATAACTTGTGGTGAGGTGACCGCCTTACTCACTCCTTGCATTCCATTTGGAGTGTTGGGAGGGATTGTGCCACCAGATTGTTGTGCCGGGATCAAAGGGCTCAACGCTGCACAAAATACAACAGAGGATCGAAGAATTGCTTGTACTTGCATTCAAGAAGGAGCAGCTAGGATTCCTCTGATTGATTATGATCGTATAAACACGCTCGGCGATCTTTGTGGTTCTCCTTGTCCCTACAAAGTTTATCCATCTACTGACTGCTCTCAGGTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

120

Amino Acids

12.72

Weight (kDa)

4.61

Isoelectric Point (pI)

30.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 116 1.3e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 108
AclWI GGATC 2 cut(s) 185, 222
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 232
AgsI TTSAA 2 cut(s) 14, 242
AluBI AGCT 2 cut(s) 254, 360
AluI AGCT 2 cut(s) 254, 360
AlwI GGATC 2 cut(s) 185, 222
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 194, 251
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 3 cut(s) 70, 110, 115
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 181, 263
BccI CCATC 1 cut(s) 343
BcnI CCSGG 1 cut(s) 175
BfaI CTAG 1 cut(s) 255
BisI GCNGC 2 cut(s) 195, 252
BlsI GCNGC 2 cut(s) 196, 253
Bme1390I CCNGG 1 cut(s) 175
BmrFI CCNGG 1 cut(s) 175
BpuMI CCSGG 1 cut(s) 175
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 51
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse1I ACTGG 1 cut(s) 48
Bse8I GATNNNNATC 1 cut(s) 276
BseDI CCNNGG 1 cut(s) 51
BseJI GATNNNNATC 1 cut(s) 276
BseNI ACTGG 1 cut(s) 48
BseXI GCAGC 2 cut(s) 181, 263
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 303
BsmI GAATGC 2 cut(s) 126, 237
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 4 cut(s) 177, 214, 277, 298
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 1 cut(s) 108
BspANI GGCC 1 cut(s) 5
BspPI GGATC 2 cut(s) 185, 222
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 4 cut(s) 177, 214, 277, 298
BssT1I CCWWGG 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 351
BstDSI CCRYGG 1 cut(s) 51
BstEII GGTNACC 1 cut(s) 103
BstKTI GATC 4 cut(s) 180, 217, 280, 301
BstMBI GATC 4 cut(s) 177, 214, 277, 298
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstPI GGTNACC 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 173
BstV1I GCAGC 2 cut(s) 181, 263
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 51
Csp6I GTAC 1 cut(s) 231
CviAII CATG 2 cut(s) 34, 52
CviJI RGCY 5 cut(s) 5, 50, 187, 254, 360
CviKI_1 RGCY 5 cut(s) 5, 50, 187, 254, 360
CviQI GTAC 1 cut(s) 231
DdeI CTNAG 1 cut(s) 351
DpnI GATC 4 cut(s) 179, 216, 279, 300
DpnII GATC 4 cut(s) 177, 214, 277, 298
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 189
Eco91I GGTNACC 1 cut(s) 103
EcoO65I GGTNACC 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 2 cut(s) 37, 55
FaiI YATR 7 cut(s) 21, 35, 53, 72, 89, 276, 284
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FaqI GGGAC 1 cut(s) 303
FatI CATG 2 cut(s) 33, 51
Fnu4HI GCNGC 2 cut(s) 195, 252
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 195, 252
FspBI CTAG 1 cut(s) 255
GluI GCNGC 2 cut(s) 195, 252
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 174
Hin1II CATG 2 cut(s) 37, 55
HinfI GANTC 1 cut(s) 259
HpaII CCGG 1 cut(s) 174
HphI GGTGA 3 cut(s) 70, 110, 115
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 1 cut(s) 242
HpyAV CCTTC 1 cut(s) 239
HpyCH4V TGCA 4 cut(s) 83, 126, 197, 237
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 1 cut(s) 351
Hsp92II CATG 2 cut(s) 37, 55
Kzo9I GATC 4 cut(s) 177, 214, 277, 298
LmnI GCTCC 1 cut(s) 248
LpnPI CCDG 5 cut(s) 19, 29, 174, 187, 338
Lsp1109I GCAGC 2 cut(s) 181, 263
MaeI CTAG 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 58, 103
MalI GATC 4 cut(s) 179, 216, 279, 300
MboI GATC 4 cut(s) 177, 214, 277, 298
MboII GAAGA 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 222
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 94, 140, 205, 273
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 175
Mva1269I GAATGC 2 cut(s) 126, 237
MwoI GCNNNNNNNGC 1 cut(s) 80
NciI CCSGG 1 cut(s) 175
NcoI CCATGG 1 cut(s) 51
NdeII GATC 4 cut(s) 177, 214, 277, 298
NlaIII CATG 2 cut(s) 37, 55
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 2 cut(s) 58, 103
PctI GAATGC 2 cut(s) 126, 237
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 2 cut(s) 196, 253
PspEI GGTNACC 1 cut(s) 103
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
RseI CAYNNNNRTG 1 cut(s) 56
SatI GCNGC 2 cut(s) 195, 252
Sau3AI GATC 4 cut(s) 177, 214, 277, 298
ScrFI CCNGG 1 cut(s) 175
SduI GDGCHC 1 cut(s) 189
SetI ASST 4 cut(s) 105, 256, 357, 362
SmiMI CAYNNNNRTG 1 cut(s) 56
Sse9I AATT 1 cut(s) 222
SsiI CCGC 1 cut(s) 108
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 173
StyI CCWWGG 1 cut(s) 51
TaqI TCGA 1 cut(s) 217
TasI AATT 1 cut(s) 222
TatI WGTACW 1 cut(s) 230
TfiI GAWTC 1 cut(s) 259
TseFI GTSAC 2 cut(s) 58, 103
TseI GCWGC 2 cut(s) 194, 251
Tsp45I GTSAC 2 cut(s) 58, 103
TspDTI ATGAA 1 cut(s) 22
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.