RLG00000036365

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003691
Physical Location & Seq
Forward (+)
79707157 .. 79707616
460 bp
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UTR
Exon/CDS
Intron
RLM00000036365

Sequence Viewer

Length: 363 bp
ATGGCCAGGTTTGCAGGCTATGGGGTGACATTCTTGGTGGTACTAGTATCTGTTCTAGTGACGACTCCATATGTCATTAAGGCATCTGTAACTTGCGAGCAGGTCGTAAACTATCTGACTCCATGCATCCCTTTTGGAGTGTTTGGAGGGAGCGTGCCAGAGGAGTGTTGTGCCGGCGTAAAAGGGCTTCATGATGCAGAAGTCACGACAGAGGATCGGAGAACTGCTTGCGGTTGCATTCAGAAAGGGTGTGCAATGATTCCTGGTCTCGACTATGACCGTGTAAACACGCTTGGTGATCTGTGTGGTGCTCCTTGTCCTTACAAAGTTTACCCTTCTACTAATTGCTCTCAGGTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

121

Amino Acids

12.71

Weight (kDa)

5.18

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 116 4e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 91
AciI CCGC 1 cut(s) 231
AclWI GGATC 1 cut(s) 222
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 42
AhlI ACTAGT 1 cut(s) 43
AjnI CCWGG 2 cut(s) 5, 262
AluBI AGCT 1 cut(s) 360
AluI AGCT 1 cut(s) 360
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 272
AlwI GGATC 1 cut(s) 222
AoxI GGCC 1 cut(s) 3
AsuHPI GGTGA 2 cut(s) 37, 308
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 171, 203
Bbv12I GWGCWC 1 cut(s) 313
BciT130I CCWGG 2 cut(s) 7, 264
BcoDI GTCTC 1 cut(s) 272
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 2 cut(s) 44, 56
BfuAI ACCTGC 1 cut(s) 91
Bme1390I CCNGG 2 cut(s) 7, 264
BmrFI CCNGG 2 cut(s) 7, 264
BmsI GCATC 3 cut(s) 92, 135, 184
BsaI GGTCTC 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse118I RCCGGY 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 2 cut(s) 7, 264
BseGI GGATG 1 cut(s) 126
BseMI GCAATG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 176
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 2 cut(s) 214, 298
BspACI CCGC 1 cut(s) 231
BspANI GGCC 1 cut(s) 5
BspHI TCATGA 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 91
BspPI GGATC 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssMI GATC 2 cut(s) 214, 298
Bst2UI CCWGG 2 cut(s) 7, 264
Bst4CI ACNGT 1 cut(s) 281
BstC8I GCNNGC 5 cut(s) 16, 98, 155, 175, 229
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 126
BstKTI GATC 2 cut(s) 217, 301
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 2 cut(s) 214, 298
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 7, 264
BstSCI CCNGG 2 cut(s) 5, 262
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 91
Cac8I GCNNGC 5 cut(s) 16, 98, 155, 175, 229
CciI TCATGA 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 173
Csp6I GTAC 1 cut(s) 41
CviAII CATG 2 cut(s) 123, 191
CviJI RGCY 4 cut(s) 5, 18, 187, 360
CviKI_1 RGCY 4 cut(s) 5, 18, 187, 360
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 1 cut(s) 351
DpnI GATC 2 cut(s) 216, 300
DpnII GATC 2 cut(s) 214, 298
EaeI YGGCCR 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 5, 262
EcoT22I ATGCAT 1 cut(s) 128
FaeI CATG 2 cut(s) 126, 194
FaiI YATR 6 cut(s) 21, 70, 72, 124, 192, 276
FatI CATG 2 cut(s) 122, 190
FauNDI CATATG 1 cut(s) 70
FokI GGATG 1 cut(s) 113
FspBI CTAG 2 cut(s) 44, 56
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 174
Hin1II CATG 2 cut(s) 126, 194
HinfI GANTC 3 cut(s) 64, 118, 259
HpaII CCGG 1 cut(s) 174
HphI GGTGA 2 cut(s) 37, 308
Hpy166II GTNNAC 3 cut(s) 109, 286, 331
Hpy188I TCNGA 3 cut(s) 117, 219, 243
Hpy188III TCNNGA 3 cut(s) 191, 205, 269
Hpy8I GTNNAC 3 cut(s) 109, 286, 331
HpyAV CCTTC 1 cut(s) 345
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4V TGCA 5 cut(s) 14, 126, 197, 237, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 351
Hsp92II CATG 2 cut(s) 126, 194
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 214, 298
LmnI GCTCC 2 cut(s) 150, 316
LpnPI CCDG 7 cut(s) 19, 86, 171, 187, 249, 276, 338
LweI GCATC 3 cut(s) 92, 135, 184
MaeI CTAG 2 cut(s) 44, 56
MaeIII GTNAC 4 cut(s) 25, 58, 88, 202
MalI GATC 2 cut(s) 216, 300
MboI GATC 2 cut(s) 214, 298
MhlI GDGCHC 1 cut(s) 313
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 58, 112
MnlI CCTC 3 cut(s) 140, 154, 205
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 128
MroNI GCCGGC 1 cut(s) 173
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 78
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 7, 264
Mva1269I GAATGC 1 cut(s) 237
MvaI CCWGG 2 cut(s) 7, 264
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 175
NdeI CATATG 1 cut(s) 70
NdeII GATC 2 cut(s) 214, 298
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 2 cut(s) 126, 194
NmuCI GTSAC 3 cut(s) 25, 58, 202
NsiI ATGCAT 1 cut(s) 128
PagI TCATGA 1 cut(s) 190
PctI GAATGC 1 cut(s) 237
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 259
PleI GAGTC 2 cut(s) 58, 112
PpsI GAGTC 2 cut(s) 58, 112
Psp6I CCWGG 2 cut(s) 5, 262
PspGI CCWGG 2 cut(s) 5, 262
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 78
Sau3AI GATC 2 cut(s) 214, 298
SchI GAGTC 2 cut(s) 58, 112
ScrFI CCNGG 2 cut(s) 7, 264
SduI GDGCHC 1 cut(s) 313
SetI ASST 4 cut(s) 11, 105, 357, 362
SfaNI GCATC 3 cut(s) 92, 135, 184
SpeI ACTAGT 1 cut(s) 43
Sse9I AATT 1 cut(s) 343
SsiI CCGC 1 cut(s) 231
SspMI CTAG 2 cut(s) 44, 56
StyD4I CCNGG 2 cut(s) 5, 262
TaaI ACNGT 1 cut(s) 281
TaqI TCGA 1 cut(s) 270
TasI AATT 1 cut(s) 343
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseFI GTSAC 3 cut(s) 25, 58, 202
Tsp45I GTSAC 3 cut(s) 25, 58, 202
TspDTI ATGAA 1 cut(s) 179
XspI CTAG 2 cut(s) 44, 56
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.