RLG00000036368

Non-specific lipid-transfer protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003691
Physical Location & Seq
Forward (+)
79738497 .. 79738853
357 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036368

Sequence Viewer

Length: 357 bp
ATGGCCAGATTTGAATTCTATGGGTTTGCTTTCATGGTGTTACTAGTATCCATGCTGGTGACTACTCCTTATGTCACTAATGCAACCATAACTTGTGGTGAGGTGGTCGCCTTACTCACTCCTTGCATTCCATTTGGAGTGCTGGGAGGGATTGTGCCACCAGATTGTTGTGCCGGGATCAAAGGGCTCAATGCTGCACAAAATACGACAGAGGATCGAAGAACTGCTTGTACTTGCATTCAAGAAGGAGCAGCTATGATTCCTGGGATTGATTATGATCGTATAAACACGCTCGGCGATCTTTGTGGTTCTCCTTGTCCCTACAAAGTTTATCCATCTACTGACTGCTCGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

119

Amino Acids

12.54

Weight (kDa)

4.42

Isoelectric Point (pI)

31.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 116 7e-08 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 185, 222
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 14
AfaI GTAC 1 cut(s) 232
AgsI TTSAA 2 cut(s) 14, 242
AhlI ACTAGT 1 cut(s) 43
AjnI CCWGG 1 cut(s) 262
AluBI AGCT 1 cut(s) 254
AluI AGCT 1 cut(s) 254
AlwI GGATC 2 cut(s) 185, 222
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 194, 251
ApoI RAATTY 1 cut(s) 14
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 2 cut(s) 70, 110
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 181, 263
BccI CCATC 1 cut(s) 343
BciT130I CCWGG 1 cut(s) 264
BciVI GTATCC 1 cut(s) 58
BcnI CCSGG 1 cut(s) 175
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 1 cut(s) 44
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 2 cut(s) 195, 252
BlsI GCNGC 2 cut(s) 196, 253
Bme1390I CCNGG 2 cut(s) 175, 264
BmrFI CCNGG 2 cut(s) 175, 264
BpuMI CCSGG 1 cut(s) 175
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 263
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 263
BseJI GATNNNNATC 1 cut(s) 276
BseXI GCAGC 2 cut(s) 181, 263
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 174
BslFI GGGAC 1 cut(s) 303
BsmFI GGGAC 1 cut(s) 303
BsmI GAATGC 2 cut(s) 126, 237
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 4 cut(s) 177, 214, 277, 298
BspANI GGCC 1 cut(s) 5
BspPI GGATC 2 cut(s) 185, 222
BssECI CCNNGG 1 cut(s) 263
BssMI GATC 4 cut(s) 177, 214, 277, 298
Bst2UI CCWGG 1 cut(s) 264
BstC8I GCNNGC 1 cut(s) 350
BstKTI GATC 4 cut(s) 180, 217, 280, 301
BstMBI GATC 4 cut(s) 177, 214, 277, 298
BstNI CCWGG 1 cut(s) 264
BstSCI CCNGG 2 cut(s) 173, 262
BstV1I GCAGC 2 cut(s) 181, 263
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 1 cut(s) 5
Cac8I GCNNGC 1 cut(s) 350
Csp6I GTAC 1 cut(s) 231
CviAII CATG 2 cut(s) 34, 52
CviJI RGCY 3 cut(s) 5, 187, 254
CviKI_1 RGCY 3 cut(s) 5, 187, 254
CviQI GTAC 1 cut(s) 231
DpnI GATC 4 cut(s) 179, 216, 279, 300
DpnII GATC 4 cut(s) 177, 214, 277, 298
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 189
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 1 cut(s) 262
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 2 cut(s) 37, 55
FaiI YATR 8 cut(s) 21, 35, 53, 72, 89, 257, 276, 284
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FaqI GGGAC 1 cut(s) 303
FatI CATG 2 cut(s) 33, 51
Fnu4HI GCNGC 2 cut(s) 195, 252
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 195, 252
FspBI CTAG 1 cut(s) 44
GluI GCNGC 2 cut(s) 195, 252
GsaI CCCAGC 1 cut(s) 146
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 174
Hin1II CATG 2 cut(s) 37, 55
HinfI GANTC 1 cut(s) 259
HpaII CCGG 1 cut(s) 174
HphI GGTGA 2 cut(s) 70, 110
Hpy188III TCNNGA 1 cut(s) 242
HpyAV CCTTC 1 cut(s) 239
HpyCH4V TGCA 4 cut(s) 83, 126, 197, 237
Hsp92II CATG 2 cut(s) 37, 55
Kzo9I GATC 4 cut(s) 177, 214, 277, 298
LmnI GCTCC 1 cut(s) 248
LpnPI CCDG 7 cut(s) 19, 41, 128, 174, 187, 249, 276
Lsp1109I GCAGC 2 cut(s) 181, 263
MaeI CTAG 1 cut(s) 44
MaeIII GTNAC 3 cut(s) 39, 58, 73
MalI GATC 4 cut(s) 179, 216, 279, 300
MboI GATC 4 cut(s) 177, 214, 277, 298
MboII GAAGA 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 14
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 3 cut(s) 94, 140, 205
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 175, 264
Mva1269I GAATGC 2 cut(s) 126, 237
MvaI CCWGG 1 cut(s) 264
NciI CCSGG 1 cut(s) 175
NdeII GATC 4 cut(s) 177, 214, 277, 298
NlaIII CATG 2 cut(s) 37, 55
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 2 cut(s) 58, 73
PctI GAATGC 2 cut(s) 126, 237
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 2 cut(s) 196, 253
Psp6I CCWGG 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 1 cut(s) 262
PsrI GAACNNNNNNTAC 2 cut(s) 214, 246
RsaI GTAC 1 cut(s) 232
RsaNI GTAC 1 cut(s) 231
RseI CAYNNNNRTG 1 cut(s) 56
SatI GCNGC 2 cut(s) 195, 252
Sau3AI GATC 4 cut(s) 177, 214, 277, 298
ScrFI CCNGG 2 cut(s) 175, 264
SduI GDGCHC 1 cut(s) 189
SetI ASST 2 cut(s) 105, 256
SmiMI CAYNNNNRTG 1 cut(s) 56
SpeI ACTAGT 1 cut(s) 43
Sse9I AATT 1 cut(s) 14
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 2 cut(s) 173, 262
TaqI TCGA 1 cut(s) 217
TasI AATT 1 cut(s) 14
TatI WGTACW 1 cut(s) 230
TfiI GAWTC 1 cut(s) 259
TseFI GTSAC 2 cut(s) 58, 73
TseI GCWGC 2 cut(s) 194, 251
Tsp45I GTSAC 2 cut(s) 58, 73
TspDTI ATGAA 1 cut(s) 22
XapI RAATTY 1 cut(s) 14
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.