MD03G1052100.v1.1

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003691
Physical Location & Seq
Reverse (-)
4159800 .. 4160162
363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1052100.v1.1.491

Sequence Viewer

Length: 363 bp
ATGGCAGGGCCTTCGGTGTCTGGGGCGGCATTCACGGTGATGTTGCTATGCATGCTGGTGACTGCTCGTTACGCAGTTAATGCAACTGTAACTTGCGGGCAAGTTGTCCAGTTACTCACTCCATGCATCCCCTTTGGAGTGTTTGGAGGGGACGTGCCACTGGATTGTTGTGCCGGCATAAAAGGGATGCATGATATACAAAAAACCGAGGAGGATCGAAGAACTGCGTGCAGTTGCATTCAACAAGGCGCTGCAATGATTCCGGGGCTAGATTATGACCGAGTTAACACGCTTGGCGATCGATGTAGTTCTCCTTGCCCGGTTTACCCCTCTACTAACTGCTCTGAGAAAGCCTCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

121

Amino Acids

12.57

Weight (kDa)

5.11

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 114 2.9e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 26, 96
AclWI GGATC 1 cut(s) 222
AgsI TTSAA 1 cut(s) 242
AjiI CACGTC 1 cut(s) 154
AlwI GGATC 1 cut(s) 222
AoxI GGCC 1 cut(s) 8
ApeKI GCWGC 1 cut(s) 251
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 8
AsuC2I CCSGG 2 cut(s) 264, 320
AsuHPI GGTGA 2 cut(s) 49, 70
BauI CACGAG 1 cut(s) 355
BbvI GCAGC 1 cut(s) 238
BcgI CGANNNNNNTGC 1 cut(s) 28
BcnI CCSGG 2 cut(s) 264, 320
BfaI CTAG 1 cut(s) 269
BfoI RGCGCY 1 cut(s) 252
BisI GCNGC 2 cut(s) 27, 252
BlsI GCNGC 2 cut(s) 28, 253
Bme1390I CCNGG 2 cut(s) 264, 320
BmgBI CACGTC 1 cut(s) 154
BmgT120I GGNCC 1 cut(s) 8
BmrFI CCNGG 2 cut(s) 264, 320
BmsI GCATC 2 cut(s) 135, 177
BplI GAGNNNNNCTC 1 cut(s) 338
BpuMI CCSGG 2 cut(s) 264, 320
Bsa29I ATCGAT 1 cut(s) 301
BsaJI CCNNGG 2 cut(s) 207, 263
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 2 cut(s) 109, 165
Bse3DI GCAATG 1 cut(s) 261
BseCI ATCGAT 1 cut(s) 301
BseDI CCNNGG 2 cut(s) 207, 263
BseGI GGATG 2 cut(s) 126, 192
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 2 cut(s) 109, 165
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 1 cut(s) 238
BsgI GTGCAG 1 cut(s) 250
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 301
BsiEI CGRYCG 1 cut(s) 301
BsiSI CCGG 3 cut(s) 174, 263, 320
BslFI GGGAC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 2 cut(s) 29, 237
BsnI GGCC 1 cut(s) 10
Bsp143I GATC 2 cut(s) 214, 298
BspACI CCGC 2 cut(s) 26, 96
BspANI GGCC 1 cut(s) 10
BspCNI CTCAG 1 cut(s) 337
BspDI ATCGAT 1 cut(s) 301
BspPI GGATC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 2 cut(s) 109, 165
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 207, 263
BssMI GATC 2 cut(s) 214, 298
BssSI CACGAG 1 cut(s) 355
Bst2BI CACGAG 1 cut(s) 355
Bst4CI ACNGT 2 cut(s) 37, 88
BstAPI GCANNNNNTGC 1 cut(s) 80
BstC8I GCNNGC 4 cut(s) 53, 98, 175, 229
BstDEI CTNAG 1 cut(s) 345
BstF5I GGATG 2 cut(s) 126, 192
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 2 cut(s) 217, 301
BstMBI GATC 2 cut(s) 214, 298
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 3 cut(s) 52, 71, 80
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 2 cut(s) 262, 318
BstV1I GCAGC 1 cut(s) 238
Bsu15I ATCGAT 1 cut(s) 301
BsuRI GGCC 1 cut(s) 10
BsuTUI ATCGAT 1 cut(s) 301
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 2 cut(s) 126, 192
BtsIMutI CAGTG 1 cut(s) 158
Cac8I GCNNGC 4 cut(s) 53, 98, 175, 229
CfoI GCGC 1 cut(s) 251
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 8
ClaI ATCGAT 1 cut(s) 301
CviAII CATG 3 cut(s) 52, 123, 191
CviJI RGCY 3 cut(s) 10, 268, 353
CviKI_1 RGCY 3 cut(s) 10, 268, 353
DdeI CTNAG 1 cut(s) 345
DpnI GATC 2 cut(s) 216, 300
DpnII GATC 2 cut(s) 214, 298
EcoO109I RGGNCCY 1 cut(s) 8
EcoT22I ATGCAT 3 cut(s) 53, 128, 192
FaeI CATG 3 cut(s) 55, 126, 194
FaiI YATR 7 cut(s) 49, 53, 124, 179, 192, 197, 276
FaqI GGGAC 1 cut(s) 164
FatI CATG 3 cut(s) 51, 122, 190
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 2 cut(s) 27, 252
FokI GGATG 2 cut(s) 113, 199
Fsp4HI GCNGC 2 cut(s) 27, 252
FspBI CTAG 1 cut(s) 269
GlaI GCGC 1 cut(s) 250
GluI GCNGC 2 cut(s) 27, 252
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 1 cut(s) 10
HapII CCGG 3 cut(s) 174, 263, 320
HhaI GCGC 1 cut(s) 251
Hin1II CATG 3 cut(s) 55, 126, 194
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 1 cut(s) 259
HpaI GTTAAC 1 cut(s) 286
HpaII CCGG 3 cut(s) 174, 263, 320
HphI GGTGA 2 cut(s) 49, 70
Hpy166II GTNNAC 2 cut(s) 286, 325
Hpy188I TCNGA 1 cut(s) 346
Hpy8I GTNNAC 2 cut(s) 286, 325
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 2 cut(s) 37, 88
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 7 cut(s) 51, 83, 126, 190, 231, 237, 254
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 71, 80
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 3 cut(s) 55, 126, 194
HspAI GCGC 1 cut(s) 249
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
KspAI GTTAAC 1 cut(s) 286
Kzo9I GATC 2 cut(s) 214, 298
LpnPI CCDG 7 cut(s) 6, 41, 122, 146, 187, 276, 333
Lsp1109I GCAGC 1 cut(s) 238
LweI GCATC 2 cut(s) 135, 177
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 4 cut(s) 58, 68, 88, 111
MalI GATC 2 cut(s) 216, 300
MboI GATC 2 cut(s) 214, 298
MboII GAAGA 1 cut(s) 231
MnlI CCTC 4 cut(s) 140, 202, 205, 340
Mph1103I ATGCAT 3 cut(s) 53, 128, 192
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 2 cut(s) 78, 285
MslI CAYNNNNRTG 2 cut(s) 38, 56
MspI CCGG 3 cut(s) 174, 263, 320
MspR9I CCNGG 2 cut(s) 264, 320
Mva1269I GAATGC 2 cut(s) 29, 237
MwoI GCNNNNNNNGC 3 cut(s) 52, 71, 80
NaeI GCCGGC 1 cut(s) 175
NciI CCSGG 2 cut(s) 264, 320
NdeII GATC 2 cut(s) 214, 298
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 3 cut(s) 55, 126, 194
NmuCI GTSAC 1 cut(s) 58
NsiI ATGCAT 3 cut(s) 53, 128, 192
NspI RCATGY 1 cut(s) 55
PaeI GCATGC 1 cut(s) 55
PctI GAATGC 2 cut(s) 29, 237
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 2 cut(s) 28, 253
Ple19I CGATCG 1 cut(s) 301
PspPI GGNCC 1 cut(s) 8
PvuI CGATCG 1 cut(s) 301
RseI CAYNNNNRTG 2 cut(s) 38, 56
SaqAI TTAA 2 cut(s) 78, 285
SatI GCNGC 2 cut(s) 27, 252
Sau3AI GATC 2 cut(s) 214, 298
Sau96I GGNCC 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 264, 320
SetI ASST 1 cut(s) 156
SfaNI GCATC 2 cut(s) 135, 177
SmiMI CAYNNNNRTG 2 cut(s) 38, 56
SphI GCATGC 1 cut(s) 55
SsiI CCGC 2 cut(s) 26, 96
SspMI CTAG 1 cut(s) 269
StyD4I CCNGG 2 cut(s) 262, 318
TaaI ACNGT 2 cut(s) 37, 88
TaiI ACGT 1 cut(s) 156
TaqI TCGA 2 cut(s) 217, 301
TaqII GACCGA 1 cut(s) 294
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 2 cut(s) 78, 285
Tru9I TTAA 2 cut(s) 78, 285
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 58
TspRI CASTG 1 cut(s) 165
XceI RCATGY 1 cut(s) 55
XspI CTAG 1 cut(s) 269
Zsp2I ATGCAT 3 cut(s) 53, 128, 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.