MD11G1054200.v1.1

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003691
Physical Location & Seq
Reverse (-)
4623572 .. 4623877
306 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1054200.v1.1.491

Sequence Viewer

Length: 306 bp
ATGCTGGTGACTGCTCCTTACACCGTTAATGCAACGGTAACTTGTGGTCAGGTTGTCACCTTACTCACTCCATGCATCCCCTTTGGAGTGTTTGGAGGGGACGTGCCACCGGATTGTTGTGCCGGCATAAAAGGCCTGCATGATATACAAAACACCGCGGAGGATCGAAGAACTGCGTGCAGCTGCATTCAACAAGGGGCTGCAATGATTCCAGGATTAGATTATGACCGAGTTAACACGCTTGGGGATCGATGTGGTTCTCCTTGCCCCTACACAGTTTACCCCTCTACTAACTGCTCCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

102

Amino Acids

10.62

Weight (kDa)

4.58

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 15 - 99 4.8e-09 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 158
AciI CCGC 2 cut(s) 156, 158
AclWI GGATC 2 cut(s) 171, 255
AgsI TTSAA 1 cut(s) 191
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 211
AluBI AGCT 1 cut(s) 183
AluI AGCT 1 cut(s) 183
AlwI GGATC 2 cut(s) 171, 255
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 3 cut(s) 180, 183, 200
AsuHPI GGTGA 2 cut(s) 19, 49
BbvI GCAGC 3 cut(s) 170, 187, 192
BciT130I CCWGG 1 cut(s) 213
BisI GCNGC 3 cut(s) 181, 184, 201
BlsI GCNGC 3 cut(s) 182, 185, 202
Bme1390I CCNGG 1 cut(s) 213
BmgBI CACGTC 1 cut(s) 103
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 1 cut(s) 84
Bsa29I ATCGAT 1 cut(s) 250
BsaJI CCNNGG 1 cut(s) 156
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 2 cut(s) 87, 117
Bse118I RCCGGY 1 cut(s) 122
Bse3DI GCAATG 1 cut(s) 210
BseBI CCWGG 1 cut(s) 213
BseCI ATCGAT 1 cut(s) 250
BseDI CCNNGG 1 cut(s) 156
BseGI GGATG 1 cut(s) 75
BseMI GCAATG 1 cut(s) 210
BseXI GCAGC 3 cut(s) 170, 187, 192
BsgI GTGCAG 1 cut(s) 199
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 1 cut(s) 135
BshVI ATCGAT 1 cut(s) 250
BsiSI CCGG 2 cut(s) 110, 123
BslFI GGGAC 1 cut(s) 113
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 1 cut(s) 135
Bsp143I GATC 2 cut(s) 163, 247
BspACI CCGC 2 cut(s) 156, 158
BspANI GGCC 1 cut(s) 135
BspDI ATCGAT 1 cut(s) 250
BspFNI CGCG 1 cut(s) 158
BspPI GGATC 2 cut(s) 171, 255
BsrDI GCAATG 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 156
BssMI GATC 2 cut(s) 163, 247
Bst2UI CCWGG 1 cut(s) 213
Bst4CI ACNGT 3 cut(s) 25, 37, 277
BstC8I GCNNGC 3 cut(s) 124, 137, 178
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 1 cut(s) 75
BstFNI CGCG 1 cut(s) 158
BstKTI GATC 2 cut(s) 166, 250
BstMBI GATC 2 cut(s) 163, 247
BstMWI GCNNNNNNNGC 1 cut(s) 132
BstNI CCWGG 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 211
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 3 cut(s) 170, 187, 192
Bsu15I ATCGAT 1 cut(s) 250
BsuRI GGCC 1 cut(s) 135
BsuTUI ATCGAT 1 cut(s) 250
BtgI CCRYGG 1 cut(s) 156
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 75
Cac8I GCNNGC 3 cut(s) 124, 137, 178
Cfr10I RCCGGY 1 cut(s) 122
Cfr42I CCGCGG 1 cut(s) 159
ClaI ATCGAT 1 cut(s) 250
CviAII CATG 2 cut(s) 72, 140
CviJI RGCY 3 cut(s) 135, 183, 200
CviKI_1 RGCY 3 cut(s) 135, 183, 200
DpnI GATC 2 cut(s) 165, 249
DpnII GATC 2 cut(s) 163, 247
Eco147I AGGCCT 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 211
EcoT22I ATGCAT 1 cut(s) 77
FaeI CATG 2 cut(s) 75, 143
FaiI YATR 5 cut(s) 73, 128, 141, 146, 225
FaqI GGGAC 1 cut(s) 113
FatI CATG 2 cut(s) 71, 139
Fnu4HI GCNGC 3 cut(s) 181, 184, 201
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 3 cut(s) 181, 184, 201
GluI GCNGC 3 cut(s) 181, 184, 201
HaeIII GGCC 1 cut(s) 135
HapII CCGG 2 cut(s) 110, 123
Hin1II CATG 2 cut(s) 75, 143
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 1 cut(s) 208
HpaI GTTAAC 1 cut(s) 235
HpaII CCGG 2 cut(s) 110, 123
HphI GGTGA 2 cut(s) 19, 49
Hpy166II GTNNAC 2 cut(s) 235, 280
Hpy188I TCNGA 1 cut(s) 301
Hpy8I GTNNAC 2 cut(s) 235, 280
HpyCH4III ACNGT 3 cut(s) 25, 37, 277
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 6 cut(s) 32, 75, 139, 180, 186, 203
HpyF10VI GCNNNNNNNGC 1 cut(s) 132
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 2 cut(s) 75, 143
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
KspAI GTTAAC 1 cut(s) 235
KspI CCGCGG 1 cut(s) 159
Kzo9I GATC 2 cut(s) 163, 247
LmnI GCTCC 2 cut(s) 19, 302
LpnPI CCDG 6 cut(s) 35, 123, 136, 149, 198, 225
Lsp1109I GCAGC 3 cut(s) 170, 187, 192
LweI GCATC 1 cut(s) 84
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 3 cut(s) 7, 37, 55
MalI GATC 2 cut(s) 165, 249
MboI GATC 2 cut(s) 163, 247
MboII GAAGA 1 cut(s) 180
MnlI CCTC 3 cut(s) 89, 154, 295
Mph1103I ATGCAT 1 cut(s) 77
MroNI GCCGGC 1 cut(s) 122
MseI TTAA 2 cut(s) 27, 234
MspA1I CMGCKG 2 cut(s) 158, 183
MspI CCGG 2 cut(s) 110, 123
MspR9I CCNGG 1 cut(s) 213
Mva1269I GAATGC 1 cut(s) 186
MvaI CCWGG 1 cut(s) 213
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 1 cut(s) 132
NaeI GCCGGC 1 cut(s) 124
NdeII GATC 2 cut(s) 163, 247
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 2 cut(s) 75, 143
NmuCI GTSAC 2 cut(s) 7, 55
NsiI ATGCAT 1 cut(s) 77
PceI AGGCCT 1 cut(s) 135
PctI GAATGC 1 cut(s) 186
PdiI GCCGGC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 208
PfoI TCCNGGA 1 cut(s) 211
PkrI GCNGC 3 cut(s) 182, 185, 202
Psp6I CCWGG 1 cut(s) 211
PspGI CCWGG 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 183
SacII CCGCGG 1 cut(s) 159
SaqAI TTAA 2 cut(s) 27, 234
SatI GCNGC 3 cut(s) 181, 184, 201
Sau3AI GATC 2 cut(s) 163, 247
ScrFI CCNGG 1 cut(s) 213
SetI ASST 4 cut(s) 54, 62, 105, 185
SfaNI GCATC 1 cut(s) 84
Sfr303I CCGCGG 1 cut(s) 159
SgrBI CCGCGG 1 cut(s) 159
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 2 cut(s) 156, 158
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 211
TaaI ACNGT 3 cut(s) 25, 37, 277
TaiI ACGT 1 cut(s) 105
TaqI TCGA 2 cut(s) 166, 250
TaqII GACCGA 1 cut(s) 243
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 2 cut(s) 27, 234
Tru9I TTAA 2 cut(s) 27, 234
TseFI GTSAC 2 cut(s) 7, 55
TseI GCWGC 3 cut(s) 180, 183, 200
Tsp45I GTSAC 2 cut(s) 7, 55
Zsp2I ATGCAT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.