Rw5G044430

Non-specific lipid-transfer protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003691
Physical Location & Seq
Forward (+)
78107633 .. 78108441
809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044430.1

Sequence Viewer

Length: 402 bp
ATGGCCAGATTTGAAGTCTATGGGTTTGCTTTCATGATGTTACTGGTATCCATGCTGGTGACTACTCCTTATGTCAGTAATGCAACCATAACTTGCGGTGAGGTGACCACCTTACTCACTCCTTGCATTCCATTCGGAGTGTTTGGAGGGGTTGTGCCACCGGATTGTTGTGCTGGGATCAAAGGGCTCAATGCTGCAAAAAAGACAACAGAGGATCGAAGAACTGCTTGTACTTGCATTCAAGAAGGAGCTGCTAGGATTCCTGGGATTGATTATGATCGTATAAACATGCTCGGCGATCTTTGTGGTTCTCCTTCTCCCTACAAAGTTTATCCATCTACTAATTGCTCTCATTTGACCACCGTACGTGAGAGAGAGAGAGAGAGATATGCATCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

133

Amino Acids

14.52

Weight (kDa)

7.5

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 116 5.3e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 96
AclWI GGATC 2 cut(s) 185, 222
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 2 cut(s) 232, 366
AgsI TTSAA 2 cut(s) 14, 242
AjnI CCWGG 1 cut(s) 262
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
AlwI GGATC 2 cut(s) 185, 222
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 194, 251
AsuHPI GGTGA 3 cut(s) 70, 110, 115
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 181, 238
BccI CCATC 1 cut(s) 343
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 264
BciVI GTATCC 1 cut(s) 58
BfaI CTAG 1 cut(s) 255
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 2 cut(s) 195, 252
BlsI GCNGC 2 cut(s) 196, 253
Bme1390I CCNGG 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 264
BsaAI YACGTR 1 cut(s) 368
BsaBI GATNNNNATC 2 cut(s) 276, 391
BsaJI CCNNGG 1 cut(s) 263
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse1I ACTGG 1 cut(s) 48
Bse8I GATNNNNATC 2 cut(s) 276, 391
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 263
BseJI GATNNNNATC 2 cut(s) 276, 391
BseNI ACTGG 1 cut(s) 48
BseXI GCAGC 2 cut(s) 181, 238
BseYI CCCAGC 1 cut(s) 173
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 161
BsiWI CGTACG 1 cut(s) 364
BsmI GAATGC 2 cut(s) 126, 237
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 4 cut(s) 177, 214, 277, 298
BspACI CCGC 1 cut(s) 96
BspANI GGCC 1 cut(s) 5
BspHI TCATGA 1 cut(s) 33
BspPI GGATC 2 cut(s) 185, 222
BsrI ACTGG 1 cut(s) 48
BssECI CCNNGG 1 cut(s) 263
BssMI GATC 4 cut(s) 177, 214, 277, 298
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 1 cut(s) 364
BstBAI YACGTR 1 cut(s) 368
BstEII GGTNACC 1 cut(s) 103
BstKTI GATC 4 cut(s) 180, 217, 280, 301
BstMBI GATC 4 cut(s) 177, 214, 277, 298
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 292
BstPI GGTNACC 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 262
BstV1I GCAGC 2 cut(s) 181, 238
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 1 cut(s) 5
CciI TCATGA 1 cut(s) 33
Csp6I GTAC 2 cut(s) 231, 365
CviAII CATG 3 cut(s) 34, 52, 289
CviJI RGCY 3 cut(s) 5, 187, 251
CviKI_1 RGCY 3 cut(s) 5, 187, 251
CviQI GTAC 2 cut(s) 231, 365
DpnI GATC 4 cut(s) 179, 216, 279, 300
DpnII GATC 4 cut(s) 177, 214, 277, 298
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 189
Eco91I GGTNACC 1 cut(s) 103
EcoO65I GGTNACC 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 262
EcoT22I ATGCAT 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 189
FaeI CATG 3 cut(s) 37, 55, 292
FaiI YATR 9 cut(s) 21, 35, 53, 72, 89, 276, 284, 290, 390
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FatI CATG 3 cut(s) 33, 51, 288
Fnu4HI GCNGC 2 cut(s) 195, 252
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 2 cut(s) 195, 252
FspBI CTAG 1 cut(s) 255
GluI GCNGC 2 cut(s) 195, 252
GsaI CCCAGC 1 cut(s) 177
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 161
Hin1II CATG 3 cut(s) 37, 55, 292
HinfI GANTC 1 cut(s) 259
HpaII CCGG 1 cut(s) 161
HphI GGTGA 3 cut(s) 70, 110, 115
Hpy188I TCNGA 1 cut(s) 137
Hpy188III TCNNGA 2 cut(s) 34, 242
HpyAV CCTTC 2 cut(s) 239, 324
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 367
HpyCH4V TGCA 5 cut(s) 83, 126, 197, 237, 392
HpySE526I ACGT 1 cut(s) 367
Hsp92II CATG 3 cut(s) 37, 55, 292
Kzo9I GATC 4 cut(s) 177, 214, 277, 298
LmnI GCTCC 1 cut(s) 248
LpnPI CCDG 7 cut(s) 19, 29, 41, 159, 174, 249, 276
Lsp1109I GCAGC 2 cut(s) 181, 238
MaeI CTAG 1 cut(s) 255
MaeII ACGT 1 cut(s) 367
MaeIII GTNAC 3 cut(s) 39, 58, 103
MalI GATC 4 cut(s) 179, 216, 279, 300
MboI GATC 4 cut(s) 177, 214, 277, 298
MboII GAAGA 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 189
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 343
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 3 cut(s) 94, 140, 205
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 394
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 56
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 264
Mva1269I GAATGC 2 cut(s) 126, 237
MvaI CCWGG 1 cut(s) 264
NdeII GATC 4 cut(s) 177, 214, 277, 298
NlaIII CATG 3 cut(s) 37, 55, 292
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 2 cut(s) 58, 103
NsiI ATGCAT 1 cut(s) 394
NspI RCATGY 1 cut(s) 292
PagI TCATGA 1 cut(s) 33
PctI GAATGC 2 cut(s) 126, 237
PfeI GAWTC 1 cut(s) 259
Pfl23II CGTACG 1 cut(s) 364
PkrI GCNGC 2 cut(s) 196, 253
Ppu21I YACGTR 1 cut(s) 368
Psp6I CCWGG 1 cut(s) 262
PspEI GGTNACC 1 cut(s) 103
PspFI CCCAGC 1 cut(s) 173
PspGI CCWGG 1 cut(s) 262
PspLI CGTACG 1 cut(s) 364
PsrI GAACNNNNNNTAC 2 cut(s) 214, 246
RsaI GTAC 2 cut(s) 232, 366
RsaNI GTAC 2 cut(s) 231, 365
RseI CAYNNNNRTG 1 cut(s) 56
SatI GCNGC 2 cut(s) 195, 252
Sau3AI GATC 4 cut(s) 177, 214, 277, 298
ScrFI CCNGG 1 cut(s) 264
SduI GDGCHC 1 cut(s) 189
SetI ASST 4 cut(s) 105, 113, 253, 370
SmiMI CAYNNNNRTG 1 cut(s) 56
Sse9I AATT 1 cut(s) 343
SsiI CCGC 1 cut(s) 96
SspMI CTAG 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 262
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 1 cut(s) 370
TaqI TCGA 1 cut(s) 217
TasI AATT 1 cut(s) 343
TatI WGTACW 1 cut(s) 230
TfiI GAWTC 1 cut(s) 259
TseFI GTSAC 2 cut(s) 58, 103
TseI GCWGC 2 cut(s) 194, 251
Tsp45I GTSAC 2 cut(s) 58, 103
TspDTI ATGAA 1 cut(s) 22
XceI RCATGY 1 cut(s) 292
XspI CTAG 1 cut(s) 255
Zsp2I ATGCAT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.