Rorug05G0421200

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003691
Physical Location & Seq
Reverse (-)
58532499 .. 58536463
3965 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0421200.1

Sequence Viewer

Length: 2442 bp
ATGAAGGGCCTCATCTTCATCTCTCTATTCCTATTTTTAGCCTTGAATTTCTCCGTTGCAGCTGATACCATATCTCCATCACAATCCATCACCGGCTCGAATACCTTGGTTTCTTCCAACCAAAGATTTGTACTAGGACTCTTCTCCCCACCCAATTCTAGAGCATGGTATTTAGGACTATGGTACAAGAAATTTCCAGATGTTGTTGTATGGATTGCAAACAGAGAAGACCCACTTCCAGGTTCAAATGGAGCCTTGACATTATCAGAAAATGGCACCCTCATCCTTTCAGACCAAATGAACAACACAATTTGGTCCTCAAATTCTTCCAGAGTAGCCGCAAACCCAGTTGCGCAGCTTTTGGTGACGGGAAATCTTGTTGTTAGAGACCAGGCGTCTCCAACAGCAACAGCAGAGTCCGAAAGCTATGTATTTCAAAGCTTTGATTTCCCATCAAACACGCTTTTACCAGATATGAAGGTAGGGTGGGACTTCAGAACTGGCCAAAACCATTTCTTGACTAGTTGGAAAAGTGCTAGTGATCCCTCCCTTGGAGAATATACCTATAGGATTGATAACCCTGAGTTGCCTCAGCTTGTTGTTGCTCAAGGATCAAAGAAAGTGTTCAGGACAGGGCCTTGGAATGGTCTTCGATTTACAGGTACTCCTGTTTCGGTTGTTACAACAGCATCATTTTTGGTACCAATCTTTGTCTACAATACCAATGAGTTGTATTACTCATATGAGGTTACTGATGATTCTATTCTGACAAGAGTGACGCTGACCGAGTCGGGTTTGGCTCAGCGGCTAGTGATGAATGAAGGGAGCAGTGAATGGGCTGTTATGTACACTCTGCAGAACAATCAGTGTGATGATTATGGTCAGTGTGGAGCAAATGGCATTTGCAAGATTTACAAATCACCGATTTGTGAATGCTTGCAGGGGTTTGTTCCCAAATCTCCAAAGGAATGGGACGTTCTTAACTGGACAAGCGGGTGCACGAGGAGGACAGCACTGCATTGTGAGAAAGGGGAAGGATTTGTGAAAGTTAATAATGTGAAACTGCCGGACTTGTTGGATTTTAGGGTGAACAAAACCATGCTCCGCGGAGAGGAATGTGAGGCAGAGTGTTTGAGGAACTGCTCCTGTATGGCTTATGCCAATTCGAACATAACCAGTGGAGGCAAAGCATGTCTTATGTGGTTTGGCAAGCTGATTGATATGCGGGAGATCGTTGAAGATAGTGACCAGATATATATTCGGATGCCAGCTTCCGAACTAGGTGATACCAGCCAGAAACATAAAAGAGTATTGCTCGTCTCGGTCATATCATCAGCAGGTTTTGTGCTTCTTTTTCTGGGTTTGTCATGCTGCTATATTTATGTGAAGAAGAGAGAGAAGAAAATGGGCTCAAGAACTAGTGAGGAGGACTTGGAGTTGCCACTGTTTGATTATGGTAGAATTGCAACAGCCACCAATGACTTCTCCGGGACAAACAAACTGGGAGAGGGAGGTTTTGGTCCGGTTTACAAGGCCAAGTTAACACAAGAAGAGTTTGTAGCAGTAAAGAGACTCTCAAAAGATTCTGGGCAAGGTCTTGAAGAGTTTAAGAATGAAGTTACAATGATGGCTAATCTTCAACACTGGAATCTTGTTAAACTTCAGGGCTGCTGCATTGAAGGAGAAGAAAGGATGCTAATCTATGAGTACATGCCCAACAAAAGCTTGGACTGCTTTATTTTCGATGAAAACAAGAAGTTGTTGCTGAAGTGGCAAAAGCGATTTGACATAATAGTAGGAATTGCTAGAGGACTTCTCTACCTACACCAAGACTCCAGGCTAAGAATCATTCATAGGGACCTTAAGAGTAGCAACATCTTACTCGACGATGAGCTGAACCCCAAAATTTCTGACTTTGGTATAGCAAGAATTTTCGGACGCAATCAGAACGAAGCTAAAACAAAACGAGTAATTGGCACATACGGATATATGTCCCCGGAGTACGCAATTGATGGAAAATTTTCAGTGAAATCTGATGTCTTCAGCTTTGGAGTGCTTTTGTTAGAGATAGTAAGCGGCAGAAAGAACAGAGGGTTTCATCATCCTGATCACCATCATAATCTTTTGGGACATGCATGGTTATTGTGGAGACAAAACAAGGGTTTGGAGCTAATGGATATATGCCTGGAGGACTCATATGTTGAGTTTGAATTGCTAAGGTGCATTCAAGTGGGTCTGTTGTGTGTTCAAAAGCTGCCTGAAGACAGACCAGTAATGTCATCGGTGGTTCTTATGTTAAGCAATGAGGGAGCAACTTTGCCTCAGCCAAAGGAGCCTGGATTCTTTATAGAAAGAGGTTCCTTGGACTTTGATAGCTTAAGAGACCCAGGAAAAAGCAACACCGGAAACACAATTACCATTACCACAGCGGAAGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

813

Amino Acids

91.31

Weight (kDa)

6.15

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 69 - 177 3.7e-32 D-mannose binding lectin
S_locus_glycop PF00954 209 - 320 1e-27 S-locus glycoprotein domain
PAN_2 PF08276 341 - 408 2.9e-19 PAN-like domain
PK_Tyr_Ser-Thr PF07714 498 - 696 1.4e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 498 - 695 2.2e-44 Protein kinase domain
DUF3403 PF11883 768 - 813 6.9e-10 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 1328
Acc65I GGTACC 1 cut(s) 700
AccB1I GGYRCC 2 cut(s) 275, 700
AccI GTMKAC 1 cut(s) 714
AccII CGCG 1 cut(s) 1107
AciI CCGC 8 cut(s) 339, 805, 993, 1105, 1107, 1225, 2076, 2429
AclWI GGATC 2 cut(s) 536, 619
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 6 cut(s) 46, 191, 322, 1905, 1929, 2018
AcuI CTGAAG 5 cut(s) 478, 1646, 1787, 2026, 2280
AcyI GRCGYC 1 cut(s) 395
AfaI GTAC 7 cut(s) 132, 185, 664, 702, 848, 1709, 2003
AfiI CCNNNNNNNGG 4 cut(s) 239, 551, 644, 1111
AflII CTTAAG 2 cut(s) 1862, 2377
AhdI GACNNNNNGTC 1 cut(s) 394
AhlI ACTAGT 2 cut(s) 521, 1418
AjnI CCWGG 6 cut(s) 238, 390, 1835, 2184, 2335, 2386
Alw21I GWGCWC 1 cut(s) 1001
Alw26I GTCTC 6 cut(s) 381, 402, 1324, 1564, 2143, 2376
Alw44I GTGCAC 1 cut(s) 997
AlwI GGATC 2 cut(s) 536, 619
AoxI GGCC 4 cut(s) 7, 502, 635, 1533
ApaLI GTGCAC 1 cut(s) 997
ApeKI GCWGC 6 cut(s) 59, 355, 1371, 1668, 1671, 2254
ApoI RAATTY 6 cut(s) 46, 191, 322, 1905, 1929, 2018
Asp700I GAANNNNTTC 2 cut(s) 623, 2020
Asp718I GGTACC 1 cut(s) 700
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 5 cut(s) 7, 315, 635, 1520, 1858
AsuC2I CCSGG 2 cut(s) 1489, 1997
AsuHPI GGTGA 6 cut(s) 82, 376, 912, 1099, 1295, 2102
AsuII TTCGAA 1 cut(s) 1166
AvaII GGWCC 3 cut(s) 315, 1520, 1858
BaeGI GKGCMC 1 cut(s) 1001
BaeI ACNNNNGTAYC 2 cut(s) 654, 687
BalI TGGCCA 1 cut(s) 504
BanI GGYRCC 2 cut(s) 275, 700
BanII GRGCYC 1 cut(s) 1412
BauI CACGAG 1 cut(s) 1000
BbsI GAAGAC 4 cut(s) 234, 641, 2032, 2268
Bbv12I GWGCWC 1 cut(s) 1001
BbvCI CCTCAGC 2 cut(s) 591, 2322
BbvI GCAGC 6 cut(s) 71, 367, 1358, 1655, 1658, 2241
BccI CCATC 6 cut(s) 85, 95, 460, 1621, 2006, 2121
BciT130I CCWGG 6 cut(s) 240, 392, 1837, 2186, 2337, 2388
BclI TGATCA 1 cut(s) 2107
BcnI CCSGG 2 cut(s) 1489, 1997
BcoDI GTCTC 6 cut(s) 381, 402, 1324, 1564, 2143, 2376
BcuI ACTAGT 2 cut(s) 521, 1418
BfaI CTAG 9 cut(s) 134, 159, 522, 537, 809, 1280, 1419, 1806, 2436
BfmI CTRYAG 2 cut(s) 565, 854
BfrI CTTAAG 2 cut(s) 1862, 2377
BfuAI ACCTGC 1 cut(s) 1328
BisI GCNGC 9 cut(s) 60, 339, 356, 806, 1372, 1669, 1672, 2077, 2255
BlpI GCTNAGC 1 cut(s) 801
BlsI GCNGC 9 cut(s) 61, 340, 357, 807, 1373, 1670, 1673, 2078, 2256
Bme1390I CCNGG 8 cut(s) 240, 392, 1489, 1837, 1997, 2186, 2337, 2388
Bme18I GGWCC 3 cut(s) 315, 1520, 1858
BmeRI GACNNNNNGTC 1 cut(s) 394
BmgT120I GGNCC 5 cut(s) 7, 315, 635, 1520, 1858
BmiI GGNNCC 6 cut(s) 253, 277, 702, 1859, 2334, 2359
BmrFI CCNGG 8 cut(s) 240, 392, 1489, 1837, 1997, 2186, 2337, 2388
BmrI ACTGGG 2 cut(s) 341, 1511
BmsI GCATC 3 cut(s) 698, 1254, 1683
BmuI ACTGGG 2 cut(s) 341, 1511
BpiI GAAGAC 4 cut(s) 234, 641, 2032, 2268
BplI GAGNNNNNCTC 4 cut(s) 1299, 1331, 1800, 1832
BpmI CTGGAG 2 cut(s) 1819, 2207
Bpu10I CCTNAGC 3 cut(s) 591, 2216, 2322
Bpu1102I GCTNAGC 1 cut(s) 801
Bpu14I TTCGAA 1 cut(s) 1166
BpuEI CTTGAG 2 cut(s) 591, 1396
BpuMI CCSGG 2 cut(s) 1489, 1997
BsaBI GATNNNNATC 2 cut(s) 1697, 2112
BsaHI GRCGYC 1 cut(s) 395
BsaI GGTCTC 2 cut(s) 381, 2376
BsaJI CCNNGG 7 cut(s) 105, 550, 638, 1105, 1995, 2361, 2386
BsaWI WCCGGW 2 cut(s) 1522, 2402
BsaXI ACNNNNNCTCC 6 cut(s) 58, 88, 649, 679, 1817, 1847
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 551, 644, 1111
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 7 cut(s) 347, 505, 989, 1176, 1506, 1649, 2270
Bse3DI GCAATG 1 cut(s) 2308
Bse8I GATNNNNATC 2 cut(s) 1697, 2112
BseBI CCWGG 6 cut(s) 240, 392, 1837, 2186, 2337, 2388
BseDI CCNNGG 7 cut(s) 105, 550, 638, 1105, 1995, 2361, 2386
BseGI GGATG 4 cut(s) 282, 1269, 1698, 2101
BseJI GATNNNNATC 2 cut(s) 1697, 2112
BseLI CCNNNNNNNGG 4 cut(s) 239, 551, 644, 1111
BseMI GCAATG 1 cut(s) 2308
BseMII CTCAG 4 cut(s) 573, 605, 815, 2336
BseNI ACTGG 7 cut(s) 347, 505, 989, 1176, 1506, 1649, 2270
BseRI GAGGAG 2 cut(s) 1018, 1439
BseSI GKGCMC 1 cut(s) 1001
BseXI GCAGC 6 cut(s) 71, 367, 1358, 1655, 1658, 2241
Bsh1236I CGCG 1 cut(s) 1107
BshFI GGCC 4 cut(s) 9, 504, 637, 1535
BshNI GGYRCC 2 cut(s) 275, 700
BsiHKAI GWGCWC 1 cut(s) 1001
BsiSI CCGG 6 cut(s) 93, 1067, 1488, 1523, 1997, 2403
BslFI GGGAC 6 cut(s) 503, 986, 1504, 1871, 1978, 2142
BslI CCNNNNNNNGG 4 cut(s) 239, 551, 644, 1111
BsmAI GTCTC 6 cut(s) 381, 402, 1324, 1564, 2143, 2376
BsmBI CGTCTC 2 cut(s) 402, 1324
BsmFI GGGAC 6 cut(s) 503, 986, 1504, 1871, 1978, 2142
BsmI GAATGC 2 cut(s) 938, 2223
BsnI GGCC 4 cut(s) 9, 504, 637, 1535
Bso31I GGTCTC 2 cut(s) 381, 2376
Bsp119I TTCGAA 1 cut(s) 1166
Bsp1286I GDGCHC 2 cut(s) 1001, 1412
Bsp1407I TGTACA 1 cut(s) 846
Bsp143I GATC 4 cut(s) 541, 611, 1230, 2107
Bsp1720I GCTNAGC 1 cut(s) 801
BspACI CCGC 8 cut(s) 339, 805, 993, 1105, 1107, 1225, 2076, 2429
BspANI GGCC 4 cut(s) 9, 504, 637, 1535
BspCNI CTCAG 4 cut(s) 574, 604, 814, 2335
BspFNI CGCG 1 cut(s) 1107
BspLI GGNNCC 6 cut(s) 253, 277, 702, 1859, 2334, 2359
BspMAI CTGCAG 1 cut(s) 858
BspMI ACCTGC 1 cut(s) 1328
BspPI GGATC 2 cut(s) 536, 619
BspT104I TTCGAA 1 cut(s) 1166
BspT107I GGYRCC 2 cut(s) 275, 700
BspTI CTTAAG 2 cut(s) 1862, 2377
BspTNI GGTCTC 2 cut(s) 381, 2376
BsrDI GCAATG 1 cut(s) 2308
BsrFI RCCGGY 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 846
BsrI ACTGG 7 cut(s) 347, 505, 989, 1176, 1506, 1649, 2270
BssAI RCCGGY 1 cut(s) 92
BssECI CCNNGG 7 cut(s) 105, 550, 638, 1105, 1995, 2361, 2386
BssMI GATC 4 cut(s) 541, 611, 1230, 2107
BssNI GRCGYC 1 cut(s) 395
BssSI CACGAG 1 cut(s) 1000
BssT1I CCWWGG 4 cut(s) 105, 550, 638, 2361
Bst2BI CACGAG 1 cut(s) 1000
Bst2UI CCWGG 6 cut(s) 240, 392, 1837, 2186, 2337, 2388
Bst4CI ACNGT 1 cut(s) 1446
Bst6I CTCTTC 4 cut(s) 146, 1385, 1545, 1596
BstACI GRCGYC 1 cut(s) 395
BstAFI CTTAAG 2 cut(s) 1862, 2377
BstAUI TGTACA 1 cut(s) 846
BstBI TTCGAA 1 cut(s) 1166
BstC8I GCNNGC 3 cut(s) 938, 1211, 1269
BstDEI CTNAG 6 cut(s) 582, 591, 801, 1841, 2216, 2322
BstDSI CCRYGG 1 cut(s) 1105
BstF5I GGATG 4 cut(s) 282, 1269, 1698, 2101
BstFNI CGCG 1 cut(s) 1107
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 4 cut(s) 544, 614, 1233, 2110
BstMAI GTCTC 6 cut(s) 381, 402, 1324, 1564, 2143, 2376
BstMBI GATC 4 cut(s) 541, 611, 1230, 2107
BstMWI GCNNNNNNNGC 3 cut(s) 1731, 1771, 2332
BstNI CCWGG 6 cut(s) 240, 392, 1837, 2186, 2337, 2388
BstNSI RCATGY 3 cut(s) 1194, 1714, 2135
BstSCI CCNGG 8 cut(s) 238, 390, 1487, 1835, 1995, 2184, 2335, 2386
BstSFI CTRYAG 2 cut(s) 565, 854
BstSLI GKGCMC 1 cut(s) 1001
BstUI CGCG 1 cut(s) 1107
BstV1I GCAGC 6 cut(s) 71, 367, 1358, 1655, 1658, 2241
BstV2I GAAGAC 4 cut(s) 234, 641, 2032, 2268
BstXI CCANNNNNNTGG 1 cut(s) 969
BsuRI GGCC 4 cut(s) 9, 504, 637, 1535
BtgI CCRYGG 1 cut(s) 1105
BtsCI GGATG 4 cut(s) 282, 1269, 1698, 2101
BtsI GCAGTG 2 cut(s) 835, 1013
BtsIMutI CAGTG 8 cut(s) 835, 872, 890, 1013, 1183, 1442, 1642, 2031
BveI ACCTGC 1 cut(s) 1328
Cac8I GCNNGC 3 cut(s) 938, 1211, 1269
CfoI GCGC 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 5 cut(s) 7, 315, 635, 1520, 1858
Cfr42I CCGCGG 1 cut(s) 1108
CseI GACGC 3 cut(s) 384, 787, 1947
Csp6I GTAC 7 cut(s) 131, 184, 663, 701, 847, 1708, 2002
CviAII CATG 7 cut(s) 165, 1099, 1191, 1368, 1711, 2132, 2136
CviQI GTAC 7 cut(s) 131, 184, 663, 701, 847, 1708, 2002
DdeI CTNAG 6 cut(s) 582, 591, 801, 1841, 2216, 2322
DpnI GATC 4 cut(s) 543, 613, 1232, 2109
DpnII GATC 4 cut(s) 541, 611, 1230, 2107
DriI GACNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 4 cut(s) 146, 1385, 1545, 1596
Eam1105I GACNNNNNGTC 1 cut(s) 394
EarI CTCTTC 4 cut(s) 146, 1385, 1545, 1596
Eco130I CCWWGG 4 cut(s) 105, 550, 638, 2361
Eco24I GRGCYC 1 cut(s) 1412
Eco31I GGTCTC 2 cut(s) 381, 2376
Eco47I GGWCC 3 cut(s) 315, 1520, 1858
Eco57I CTGAAG 5 cut(s) 478, 1646, 1787, 2026, 2280
EcoO109I RGGNCCY 3 cut(s) 7, 635, 1858
EcoRII CCWGG 6 cut(s) 238, 390, 1835, 2184, 2335, 2386
EcoT14I CCWWGG 4 cut(s) 105, 550, 638, 2361
EcoT22I ATGCAT 1 cut(s) 2137
EcoT38I GRGCYC 1 cut(s) 1412
ErhI CCWWGG 4 cut(s) 105, 550, 638, 2361
Esp3I CGTCTC 2 cut(s) 402, 1324
FaeI CATG 7 cut(s) 168, 1102, 1194, 1371, 1714, 2135, 2139
FalI AAGNNNNNCTT 4 cut(s) 219, 251, 1179, 1211
FaqI GGGAC 6 cut(s) 503, 986, 1504, 1871, 1978, 2142
FatI CATG 7 cut(s) 164, 1098, 1190, 1367, 1710, 2131, 2135
FauI CCCGC 2 cut(s) 986, 1218
FauNDI CATATG 2 cut(s) 742, 2197
FbaI TGATCA 1 cut(s) 2107
FblI GTMKAC 1 cut(s) 714
Fnu4HI GCNGC 9 cut(s) 60, 339, 356, 806, 1372, 1669, 1672, 2077, 2255
FokI GGATG 4 cut(s) 269, 1276, 1705, 2088
FriOI GRGCYC 1 cut(s) 1412
Fsp4HI GCNGC 9 cut(s) 60, 339, 356, 806, 1372, 1669, 1672, 2077, 2255
FspBI CTAG 9 cut(s) 134, 159, 522, 537, 809, 1280, 1419, 1806, 2436
FspI TGCGCA 1 cut(s) 354
GlaI GCGC 1 cut(s) 354
GluI GCNGC 9 cut(s) 60, 339, 356, 806, 1372, 1669, 1672, 2077, 2255
GsuI CTGGAG 2 cut(s) 1819, 2207
HaeIII GGCC 4 cut(s) 9, 504, 637, 1535
HapII CCGG 6 cut(s) 93, 1067, 1488, 1523, 1997, 2403
HgaI GACGC 3 cut(s) 384, 787, 1947
HhaI GCGC 1 cut(s) 355
Hin1I GRCGYC 1 cut(s) 395
Hin1II CATG 7 cut(s) 168, 1102, 1194, 1371, 1714, 2135, 2139
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HincII GTYRAC 1 cut(s) 1542
HindII GTYRAC 1 cut(s) 1542
HindIII AAGCTT 2 cut(s) 439, 1723
HpaI GTTAAC 1 cut(s) 1542
HpaII CCGG 6 cut(s) 93, 1067, 1488, 1523, 1997, 2403
HphI GGTGA 6 cut(s) 82, 376, 912, 1099, 1295, 2102
Hpy166II GTNNAC 6 cut(s) 715, 849, 999, 1090, 1528, 1542
Hpy188III TCNNGA 8 cut(s) 159, 197, 330, 517, 628, 1413, 1598, 2105
Hpy8I GTNNAC 6 cut(s) 715, 849, 999, 1090, 1528, 1542
Hpy99I CGWCG 1 cut(s) 1889
HpyAV CCTTC 4 cut(s) 472, 815, 1028, 1673
HpyCH4III ACNGT 1 cut(s) 1446
HpyCH4IV ACGT 1 cut(s) 975
HpyF10VI GCNNNNNNNGC 3 cut(s) 1731, 1771, 2332
HpyF3I CTNAG 6 cut(s) 582, 591, 801, 1841, 2216, 2322
HpySE526I ACGT 1 cut(s) 975
Hsp92I GRCGYC 1 cut(s) 395
Hsp92II CATG 7 cut(s) 168, 1102, 1194, 1371, 1714, 2135, 2139
HspAI GCGC 1 cut(s) 353
KpnI GGTACC 1 cut(s) 704
Ksp22I TGATCA 1 cut(s) 2107
KspAI GTTAAC 1 cut(s) 1542
KspI CCGCGG 1 cut(s) 1108
Kzo9I GATC 4 cut(s) 541, 611, 1230, 2107
LmnI GCTCC 8 cut(s) 251, 825, 890, 1107, 1148, 2167, 2309, 2332
Lsp1109I GCAGC 6 cut(s) 71, 367, 1358, 1655, 1658, 2241
LweI GCATC 3 cut(s) 698, 1254, 1683
MaeI CTAG 9 cut(s) 134, 159, 522, 537, 809, 1280, 1419, 1806, 2436
MaeII ACGT 1 cut(s) 975
MaeIII GTNAC 6 cut(s) 364, 679, 748, 775, 1244, 1618
MalI GATC 4 cut(s) 543, 613, 1232, 2109
MboI GATC 4 cut(s) 541, 611, 1230, 2107
MfeI CAATTG 1 cut(s) 2007
MhlI GDGCHC 2 cut(s) 1001, 1412
MlsI TGGCCA 1 cut(s) 504
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 6 cut(s) 132, 425, 797, 1566, 1826, 2186
MmeI TCCRAC 4 cut(s) 141, 425, 506, 1056
Mox20I TGGCCA 1 cut(s) 504
Mph1103I ATGCAT 1 cut(s) 2137
MroXI GAANNNNTTC 2 cut(s) 623, 2020
MscI TGGCCA 1 cut(s) 504
MseI TTAA 8 cut(s) 981, 1050, 1541, 1608, 1656, 1863, 2297, 2378
MslI CAYNNNNRTG 1 cut(s) 2228
Msp20I TGGCCA 1 cut(s) 504
MspA1I CMGCKG 4 cut(s) 62, 805, 1107, 2429
MspCI CTTAAG 2 cut(s) 1862, 2377
MspI CCGG 6 cut(s) 93, 1067, 1488, 1523, 1997, 2403
MspR9I CCNGG 8 cut(s) 240, 392, 1489, 1837, 1997, 2186, 2337, 2388
MunI CAATTG 1 cut(s) 2007
Mva1269I GAATGC 2 cut(s) 938, 2223
MvaI CCWGG 6 cut(s) 240, 392, 1837, 2186, 2337, 2388
MvnI CGCG 1 cut(s) 1107
MwoI GCNNNNNNNGC 3 cut(s) 1731, 1771, 2332
NciI CCSGG 2 cut(s) 1489, 1997
NdeI CATATG 2 cut(s) 742, 2197
NdeII GATC 4 cut(s) 541, 611, 1230, 2107
NlaIII CATG 7 cut(s) 168, 1102, 1194, 1371, 1714, 2135, 2139
NlaIV GGNNCC 6 cut(s) 253, 277, 702, 1859, 2334, 2359
NmuCI GTSAC 3 cut(s) 364, 775, 1244
NsbI TGCGCA 1 cut(s) 354
NsiI ATGCAT 1 cut(s) 2137
NspI RCATGY 3 cut(s) 1194, 1714, 2135
NspV TTCGAA 1 cut(s) 1166
PctI GAATGC 2 cut(s) 938, 2223
PdmI GAANNNNTTC 2 cut(s) 623, 2020
PfeI GAWTC 5 cut(s) 758, 1583, 1648, 1845, 2340
PflFI GACNNNGTC 1 cut(s) 787
PfoI TCCNGGA 1 cut(s) 1487
PkrI GCNGC 9 cut(s) 61, 340, 357, 807, 1373, 1670, 1673, 2078, 2256
PleI GAGTC 6 cut(s) 132, 424, 796, 1566, 1826, 2186
PpsI GAGTC 6 cut(s) 132, 424, 796, 1566, 1826, 2186
PpuMI RGGWCCY 1 cut(s) 1858
Psp5II RGGWCCY 1 cut(s) 1858
Psp6I CCWGG 6 cut(s) 238, 390, 1835, 2184, 2335, 2386
PspGI CCWGG 6 cut(s) 238, 390, 1835, 2184, 2335, 2386
PspN4I GGNNCC 6 cut(s) 253, 277, 702, 1859, 2334, 2359
PspPI GGNCC 5 cut(s) 7, 315, 635, 1520, 1858
PspPPI RGGWCCY 1 cut(s) 1858
PstI CTGCAG 1 cut(s) 858
PsyI GACNNNGTC 1 cut(s) 787
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 7 cut(s) 132, 185, 664, 702, 848, 1709, 2003
RsaNI GTAC 7 cut(s) 131, 184, 663, 701, 847, 1708, 2002
RseI CAYNNNNRTG 1 cut(s) 2228
SacII CCGCGG 1 cut(s) 1108
SaqAI TTAA 8 cut(s) 981, 1050, 1541, 1608, 1656, 1863, 2297, 2378
SatI GCNGC 9 cut(s) 60, 339, 356, 806, 1372, 1669, 1672, 2077, 2255
Sau3AI GATC 4 cut(s) 541, 611, 1230, 2107
Sau96I GGNCC 5 cut(s) 7, 315, 635, 1520, 1858
SchI GAGTC 6 cut(s) 132, 425, 797, 1566, 1826, 2186
ScrFI CCNGG 8 cut(s) 240, 392, 1489, 1837, 1997, 2186, 2337, 2388
SduI GDGCHC 2 cut(s) 1001, 1412
SfaNI GCATC 3 cut(s) 698, 1254, 1683
SfcI CTRYAG 2 cut(s) 565, 854
Sfr303I CCGCGG 1 cut(s) 1108
SfuI TTCGAA 1 cut(s) 1166
SgrBI CCGCGG 1 cut(s) 1108
SinI GGWCC 3 cut(s) 315, 1520, 1858
SmiMI CAYNNNNRTG 1 cut(s) 2228
SmlI CTYRAG 4 cut(s) 606, 1411, 1862, 2377
SmoI CTYRAG 4 cut(s) 606, 1411, 1862, 2377
SpeI ACTAGT 2 cut(s) 521, 1418
SsiI CCGC 8 cut(s) 339, 805, 993, 1105, 1107, 1225, 2076, 2429
SspMI CTAG 9 cut(s) 134, 159, 522, 537, 809, 1280, 1419, 1806, 2436
StyD4I CCNGG 8 cut(s) 238, 390, 1487, 1835, 1995, 2184, 2335, 2386
StyI CCWWGG 4 cut(s) 105, 550, 638, 2361
TaaI ACNGT 1 cut(s) 1446
TaiI ACGT 1 cut(s) 978
TaqI TCGA 5 cut(s) 98, 652, 1166, 1743, 1884
TaqII GACCGA 2 cut(s) 800, 1312
TatI WGTACW 3 cut(s) 130, 846, 1707
TauI GCSGC 3 cut(s) 341, 808, 2079
TfiI GAWTC 5 cut(s) 758, 1583, 1648, 1845, 2340
Tru1I TTAA 8 cut(s) 981, 1050, 1541, 1608, 1656, 1863, 2297, 2378
Tru9I TTAA 8 cut(s) 981, 1050, 1541, 1608, 1656, 1863, 2297, 2378
TscAI CASTG 8 cut(s) 835, 872, 890, 1020, 1183, 1449, 1649, 2031
TseFI GTSAC 3 cut(s) 364, 775, 1244
TseI GCWGC 6 cut(s) 59, 355, 1371, 1668, 1671, 2254
Tsp45I GTSAC 3 cut(s) 364, 775, 1244
TspGWI ACGGA 2 cut(s) 43, 1998
TspRI CASTG 8 cut(s) 835, 872, 890, 1020, 1183, 1449, 1649, 2031
Tth111I GACNNNGTC 1 cut(s) 787
Vha464I CTTAAG 2 cut(s) 1862, 2377
VneI GTGCAC 1 cut(s) 997
VpaK11BI GGWCC 3 cut(s) 315, 1520, 1858
XapI RAATTY 6 cut(s) 46, 191, 322, 1905, 1929, 2018
XbaI TCTAGA 1 cut(s) 158
XceI RCATGY 3 cut(s) 1194, 1714, 2135
XcmI CCANNNNNNNNNTGG 1 cut(s) 1723
XmiI GTMKAC 1 cut(s) 714
XmnI GAANNNNTTC 2 cut(s) 623, 2020
XspI CTAG 9 cut(s) 134, 159, 522, 537, 809, 1280, 1419, 1806, 2436
Zsp2I ATGCAT 1 cut(s) 2137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.