pycom03g04140

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003691
Physical Location & Seq
Reverse (-)
3247719 .. 3248036
318 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04140.1

Sequence Viewer

Length: 318 bp
ATGGCAAGGCCTTCGGTGTCGAGGGCGGCATTCACGGTGATGTTGCTATGCATGCTGGTGACTGCTCGTTACGCCGTTAATGCAACTGTAACTTGCGGGCAGGTTGTCCAGTTACTCACTCCATGCATCCCCTTTGGAGTGTTTGCAGGGGACGTGCCACCGGATTGTTGTGCCGGCATAAAAGAGATGCATGATATTCAAAAAACCGCGGAGGATCGAAGAACTGCGTGCAGTTGCATTCAACAAGGAGCTGCAATGATTCCGGGGCTAGATTATGACCGAGTTAACACGCTTGGCGATCGATGTAATCCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

106

Amino Acids

11.28

Weight (kDa)

7.51

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 103 1.3e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 91
AccII CGCG 1 cut(s) 209
AciI CCGC 4 cut(s) 26, 96, 207, 209
AclWI GGATC 1 cut(s) 222
AgsI TTSAA 2 cut(s) 200, 242
AjiI CACGTC 1 cut(s) 154
AluBI AGCT 1 cut(s) 251
AluI AGCT 1 cut(s) 251
AlwI GGATC 1 cut(s) 222
AoxI GGCC 1 cut(s) 8
ApeKI GCWGC 1 cut(s) 251
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 2 cut(s) 49, 70
BbvI GCAGC 1 cut(s) 238
BceAI ACGGC 1 cut(s) 59
BcgI CGANNNNNNTGC 1 cut(s) 28
BcnI CCSGG 1 cut(s) 264
BfaI CTAG 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 91
BisI GCNGC 2 cut(s) 27, 252
BlsI GCNGC 2 cut(s) 28, 253
Bme1390I CCNGG 1 cut(s) 264
BmgBI CACGTC 1 cut(s) 154
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 2 cut(s) 135, 177
BpuMI CCSGG 1 cut(s) 264
Bsa29I ATCGAT 1 cut(s) 301
BsaJI CCNNGG 2 cut(s) 207, 263
BsaWI WCCGGW 1 cut(s) 160
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 109
Bse3DI GCAATG 1 cut(s) 261
BseCI ATCGAT 1 cut(s) 301
BseDI CCNNGG 2 cut(s) 207, 263
BseGI GGATG 1 cut(s) 126
BseMI GCAATG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 109
BseXI GCAGC 1 cut(s) 238
BsgI GTGCAG 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 301
BshFI GGCC 1 cut(s) 10
BshVI ATCGAT 1 cut(s) 301
BsiEI CGRYCG 1 cut(s) 301
BsiSI CCGG 3 cut(s) 161, 174, 263
BslFI GGGAC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 164
BsmI GAATGC 2 cut(s) 29, 237
BsnI GGCC 1 cut(s) 10
Bsp143I GATC 2 cut(s) 214, 298
BspACI CCGC 4 cut(s) 26, 96, 207, 209
BspANI GGCC 1 cut(s) 10
BspDI ATCGAT 1 cut(s) 301
BspFNI CGCG 1 cut(s) 209
BspMI ACCTGC 1 cut(s) 91
BspPI GGATC 1 cut(s) 222
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 173
BsrI ACTGG 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 2 cut(s) 207, 263
BssMI GATC 2 cut(s) 214, 298
Bst4CI ACNGT 2 cut(s) 37, 88
BstC8I GCNNGC 4 cut(s) 53, 98, 175, 229
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 1 cut(s) 126
BstFNI CGCG 1 cut(s) 209
BstKTI GATC 2 cut(s) 217, 301
BstMBI GATC 2 cut(s) 214, 298
BstMCI CGRYCG 1 cut(s) 301
BstMWI GCNNNNNNNGC 3 cut(s) 52, 71, 80
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 262
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 1 cut(s) 238
Bsu15I ATCGAT 1 cut(s) 301
BsuRI GGCC 1 cut(s) 10
BsuTUI ATCGAT 1 cut(s) 301
BtgI CCRYGG 1 cut(s) 207
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 91
Cac8I GCNNGC 4 cut(s) 53, 98, 175, 229
Cfr10I RCCGGY 1 cut(s) 173
Cfr42I CCGCGG 1 cut(s) 210
ClaI ATCGAT 1 cut(s) 301
CviAII CATG 3 cut(s) 52, 123, 191
CviJI RGCY 3 cut(s) 10, 251, 268
CviKI_1 RGCY 3 cut(s) 10, 251, 268
DpnI GATC 2 cut(s) 216, 300
DpnII GATC 2 cut(s) 214, 298
Eco147I AGGCCT 1 cut(s) 10
EcoT22I ATGCAT 3 cut(s) 53, 128, 192
FaeI CATG 3 cut(s) 55, 126, 194
FaiI YATR 6 cut(s) 49, 53, 124, 179, 192, 276
FaqI GGGAC 1 cut(s) 164
FatI CATG 3 cut(s) 51, 122, 190
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 2 cut(s) 27, 252
FokI GGATG 1 cut(s) 113
Fsp4HI GCNGC 2 cut(s) 27, 252
FspBI CTAG 1 cut(s) 269
GluI GCNGC 2 cut(s) 27, 252
HaeIII GGCC 1 cut(s) 10
HapII CCGG 3 cut(s) 161, 174, 263
Hin1II CATG 3 cut(s) 55, 126, 194
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HinfI GANTC 1 cut(s) 259
HpaI GTTAAC 1 cut(s) 286
HpaII CCGG 3 cut(s) 161, 174, 263
HphI GGTGA 2 cut(s) 49, 70
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 1 cut(s) 312
Hpy8I GTNNAC 1 cut(s) 286
HpyAV CCTTC 1 cut(s) 21
HpyCH4III ACNGT 2 cut(s) 37, 88
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 8 cut(s) 51, 83, 126, 146, 190, 231, 237, 254
HpyF10VI GCNNNNNNNGC 3 cut(s) 52, 71, 80
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 3 cut(s) 55, 126, 194
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
KspAI GTTAAC 1 cut(s) 286
KspI CCGCGG 1 cut(s) 210
Kzo9I GATC 2 cut(s) 214, 298
LmnI GCTCC 1 cut(s) 248
LpnPI CCDG 7 cut(s) 41, 86, 122, 132, 174, 187, 276
Lsp1109I GCAGC 1 cut(s) 238
LweI GCATC 2 cut(s) 135, 177
MaeI CTAG 1 cut(s) 269
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 4 cut(s) 58, 68, 88, 111
MalI GATC 2 cut(s) 216, 300
MboI GATC 2 cut(s) 214, 298
MboII GAAGA 1 cut(s) 231
MnlI CCTC 2 cut(s) 15, 205
Mph1103I ATGCAT 3 cut(s) 53, 128, 192
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 2 cut(s) 78, 285
MslI CAYNNNNRTG 2 cut(s) 38, 56
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 3 cut(s) 161, 174, 263
MspR9I CCNGG 1 cut(s) 264
Mva1269I GAATGC 2 cut(s) 29, 237
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 3 cut(s) 52, 71, 80
NaeI GCCGGC 1 cut(s) 175
NciI CCSGG 1 cut(s) 264
NdeII GATC 2 cut(s) 214, 298
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 3 cut(s) 55, 126, 194
NmuCI GTSAC 1 cut(s) 58
NsiI ATGCAT 3 cut(s) 53, 128, 192
NspI RCATGY 1 cut(s) 55
PaeI GCATGC 1 cut(s) 55
PceI AGGCCT 1 cut(s) 10
PctI GAATGC 2 cut(s) 29, 237
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 259
PkrI GCNGC 2 cut(s) 28, 253
Ple19I CGATCG 1 cut(s) 301
PvuI CGATCG 1 cut(s) 301
RseI CAYNNNNRTG 2 cut(s) 38, 56
SacII CCGCGG 1 cut(s) 210
SaqAI TTAA 2 cut(s) 78, 285
SatI GCNGC 2 cut(s) 27, 252
Sau3AI GATC 2 cut(s) 214, 298
ScrFI CCNGG 1 cut(s) 264
SetI ASST 3 cut(s) 105, 156, 253
SfaNI GCATC 2 cut(s) 135, 177
Sfr303I CCGCGG 1 cut(s) 210
SgrBI CCGCGG 1 cut(s) 210
SmiMI CAYNNNNRTG 2 cut(s) 38, 56
SphI GCATGC 1 cut(s) 55
SseBI AGGCCT 1 cut(s) 10
SsiI CCGC 4 cut(s) 26, 96, 207, 209
SspMI CTAG 1 cut(s) 269
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 1 cut(s) 262
TaaI ACNGT 2 cut(s) 37, 88
TaiI ACGT 1 cut(s) 156
TaqI TCGA 3 cut(s) 20, 217, 301
TaqII GACCGA 1 cut(s) 294
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 2 cut(s) 78, 285
Tru9I TTAA 2 cut(s) 78, 285
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 58
XceI RCATGY 1 cut(s) 55
XspI CTAG 1 cut(s) 269
Zsp2I ATGCAT 3 cut(s) 53, 128, 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.