Rw5G044410

Non-specific lipid-transfer protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003691
Physical Location & Seq
Forward (+)
78097612 .. 78098485
874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044410.1

Sequence Viewer

Length: 366 bp
ATGGCCAGATTTGAAGTCTCTGCACGTGGGTTTGCTTTCATGGTGCTACTGGTCATGGTGGTGACTACTCCTTATGCCACTAATGCAACCATAACTTGTGGTGAGGTGACCACCTTACTCACTCCTTGCATTCCATTTGGAGTGTTGGGAGGGATTGTGCCACCAGATTGTTGTGCCGGGATAAAAGGGCTCAACGCTGCACAAAATACAACAGAGGATCGAAGAATTGCTTGTACTTGCATTCAAGAAGGAGCAGCCAGGATTCCTCTGATTGATTATGATCGTATAAACACGCTCGGCGATCTTTGTGGTTCTCCTTGTCCCTACAAAGTTTATCCATCTACTGACTGCTCTCAGGTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

121

Amino Acids

12.83

Weight (kDa)

4.85

Isoelectric Point (pI)

30.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 33 - 117 1e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 225
AcoI YGGCCR 1 cut(s) 3
AcvI CACGTG 1 cut(s) 26
AfaI GTAC 1 cut(s) 235
AgsI TTSAA 2 cut(s) 14, 245
AjnI CCWGG 1 cut(s) 257
AluBI AGCT 1 cut(s) 363
AluI AGCT 1 cut(s) 363
Alw26I GTCTC 1 cut(s) 22
AlwI GGATC 1 cut(s) 225
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 197, 254
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 3 cut(s) 73, 113, 118
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 192
BbrPI CACGTG 1 cut(s) 26
BbvI GCAGC 2 cut(s) 184, 266
BccI CCATC 1 cut(s) 346
BciT130I CCWGG 1 cut(s) 259
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 22
BisI GCNGC 2 cut(s) 198, 255
BlsI GCNGC 2 cut(s) 199, 256
Bme1390I CCNGG 2 cut(s) 178, 259
BmrFI CCNGG 2 cut(s) 178, 259
BpuMI CCSGG 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 279
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bse1I ACTGG 1 cut(s) 54
Bse8I GATNNNNATC 1 cut(s) 279
BseBI CCWGG 1 cut(s) 259
BseJI GATNNNNATC 1 cut(s) 279
BseNI ACTGG 1 cut(s) 54
BseXI GCAGC 2 cut(s) 184, 266
BsgI GTGCAG 2 cut(s) 6, 183
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 306
BsmAI GTCTC 1 cut(s) 22
BsmFI GGGAC 1 cut(s) 306
BsmI GAATGC 2 cut(s) 129, 240
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 3 cut(s) 217, 280, 301
BspANI GGCC 1 cut(s) 5
BspPI GGATC 1 cut(s) 225
BsrI ACTGG 1 cut(s) 54
BssMI GATC 3 cut(s) 217, 280, 301
Bst2UI CCWGG 1 cut(s) 259
BstBAI YACGTR 1 cut(s) 26
BstDEI CTNAG 1 cut(s) 354
BstEII GGTNACC 1 cut(s) 106
BstKTI GATC 3 cut(s) 220, 283, 304
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 3 cut(s) 217, 280, 301
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 1 cut(s) 259
BstPI GGTNACC 1 cut(s) 106
BstSCI CCNGG 2 cut(s) 176, 257
BstV1I GCAGC 2 cut(s) 184, 266
BsuRI GGCC 1 cut(s) 5
Csp6I GTAC 1 cut(s) 234
CviAII CATG 2 cut(s) 40, 55
CviJI RGCY 4 cut(s) 5, 190, 257, 363
CviKI_1 RGCY 4 cut(s) 5, 190, 257, 363
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 1 cut(s) 354
DpnI GATC 3 cut(s) 219, 282, 303
DpnII GATC 3 cut(s) 217, 280, 301
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 192
Eco72I CACGTG 1 cut(s) 26
Eco91I GGTNACC 1 cut(s) 106
EcoO65I GGTNACC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 257
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 2 cut(s) 43, 58
FaiI YATR 6 cut(s) 41, 56, 75, 92, 279, 287
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 306
FatI CATG 2 cut(s) 39, 54
Fnu4HI GCNGC 2 cut(s) 198, 255
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 2 cut(s) 198, 255
GluI GCNGC 2 cut(s) 198, 255
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 177
Hin1II CATG 2 cut(s) 43, 58
HinfI GANTC 1 cut(s) 262
HpaII CCGG 1 cut(s) 177
HphI GGTGA 3 cut(s) 73, 113, 118
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 242
HpyCH4IV ACGT 1 cut(s) 25
HpyCH4V TGCA 5 cut(s) 23, 86, 129, 200, 240
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 1 cut(s) 354
HpySE526I ACGT 1 cut(s) 25
Hsp92II CATG 2 cut(s) 43, 58
Kzo9I GATC 3 cut(s) 217, 280, 301
LmnI GCTCC 1 cut(s) 251
LpnPI CCDG 7 cut(s) 19, 35, 177, 190, 244, 271, 341
Lsp1109I GCAGC 2 cut(s) 184, 266
MaeII ACGT 1 cut(s) 25
MaeIII GTNAC 2 cut(s) 61, 106
MalI GATC 3 cut(s) 219, 282, 303
MboI GATC 3 cut(s) 217, 280, 301
MboII GAAGA 1 cut(s) 234
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 225
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 97, 143, 208, 276
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 59
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 2 cut(s) 178, 259
Mva1269I GAATGC 2 cut(s) 129, 240
MvaI CCWGG 1 cut(s) 259
MwoI GCNNNNNNNGC 1 cut(s) 83
NciI CCSGG 1 cut(s) 178
NdeII GATC 3 cut(s) 217, 280, 301
NlaIII CATG 2 cut(s) 43, 58
NmeAIII GCCGAG 1 cut(s) 276
NmuCI GTSAC 2 cut(s) 61, 106
PctI GAATGC 2 cut(s) 129, 240
PfeI GAWTC 1 cut(s) 262
PkrI GCNGC 2 cut(s) 199, 256
PmaCI CACGTG 1 cut(s) 26
PmlI CACGTG 1 cut(s) 26
Ppu21I YACGTR 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 257
PspCI CACGTG 1 cut(s) 26
PspEI GGTNACC 1 cut(s) 106
PspGI CCWGG 1 cut(s) 257
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
RseI CAYNNNNRTG 1 cut(s) 59
SatI GCNGC 2 cut(s) 198, 255
Sau3AI GATC 3 cut(s) 217, 280, 301
ScrFI CCNGG 2 cut(s) 178, 259
SduI GDGCHC 1 cut(s) 192
SetI ASST 5 cut(s) 28, 108, 116, 360, 365
SmiMI CAYNNNNRTG 1 cut(s) 59
Sse9I AATT 1 cut(s) 225
StyD4I CCNGG 2 cut(s) 176, 257
TaiI ACGT 1 cut(s) 28
TaqI TCGA 1 cut(s) 220
TasI AATT 1 cut(s) 225
TatI WGTACW 1 cut(s) 233
TfiI GAWTC 1 cut(s) 262
TseFI GTSAC 2 cut(s) 61, 106
TseI GCWGC 2 cut(s) 197, 254
Tsp45I GTSAC 2 cut(s) 61, 106
TspDTI ATGAA 1 cut(s) 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.