Rw5G044420

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003691
Physical Location & Seq
Forward (+)
78100342 .. 78100725
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044420.1

Sequence Viewer

Length: 384 bp
ATGGCCAGGTTTGCAGGCTATGGGGTGACATTCTTGGTGGTACTAGTATCTGTTCTAGTGACGACTCCGTATGTCATTAAGGCATCTGTAACTTGCGAGCAGGTCGTAAACGATCTGACTCCATGCATCCCTTTTGGAGTGTTTGGAGGGAGCGTGCCAGAGGAGTGTTGTGCCGGCGTAAAAGGGCTTCATGATGCAGAAGTCACGACAGAGGATCGGAGAACTGCATGCGGTTGCATTCAGAAAGGGTGTGCAATGATTCCTGGTCTCGACTATGACCGTGTAAACACGCTTGGGGATCTATGTGTAATTAATTCCTCCATTATAACATACTTGGATTTACTATATATGCTTCATGTTATTATATTCATCTCCACAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

127

Amino Acids

13.67

Weight (kDa)

4.71

Isoelectric Point (pI)

32.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 32 - 105 7.8e-07 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
Acc36I ACCTGC 1 cut(s) 91
AciI CCGC 1 cut(s) 231
AclWI GGATC 2 cut(s) 222, 306
AcoI YGGCCR 1 cut(s) 3
AdeI CACNNNGTG 1 cut(s) 381
AfaI GTAC 1 cut(s) 42
AhlI ACTAGT 1 cut(s) 43
AjnI CCWGG 2 cut(s) 5, 262
Alw26I GTCTC 1 cut(s) 272
AlwI GGATC 2 cut(s) 222, 306
AoxI GGCC 1 cut(s) 3
AseI ATTAAT 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 37
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 171, 203
BciT130I CCWGG 2 cut(s) 7, 264
BcoDI GTCTC 1 cut(s) 272
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 2 cut(s) 44, 56
BfuAI ACCTGC 1 cut(s) 91
Bme1390I CCNGG 2 cut(s) 7, 264
BmrFI CCNGG 2 cut(s) 7, 264
BmsI GCATC 3 cut(s) 92, 135, 184
BsaI GGTCTC 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse118I RCCGGY 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 2 cut(s) 7, 264
BseGI GGATG 1 cut(s) 126
BseMI GCAATG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 176
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 272
Bsp143I GATC 3 cut(s) 112, 214, 298
BspACI CCGC 1 cut(s) 231
BspANI GGCC 1 cut(s) 5
BspHI TCATGA 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 91
BspPI GGATC 2 cut(s) 222, 306
BspTNI GGTCTC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssMI GATC 3 cut(s) 112, 214, 298
Bst2UI CCWGG 2 cut(s) 7, 264
Bst4CI ACNGT 1 cut(s) 281
BstC8I GCNNGC 5 cut(s) 16, 98, 155, 175, 229
BstF5I GGATG 1 cut(s) 126
BstKTI GATC 3 cut(s) 115, 217, 301
BstMAI GTCTC 1 cut(s) 272
BstMBI GATC 3 cut(s) 112, 214, 298
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 7, 264
BstNSI RCATGY 1 cut(s) 231
BstSCI CCNGG 2 cut(s) 5, 262
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 91
Cac8I GCNNGC 5 cut(s) 16, 98, 155, 175, 229
CciI TCATGA 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 173
Csp6I GTAC 1 cut(s) 41
CviAII CATG 4 cut(s) 123, 191, 228, 356
CviJI RGCY 3 cut(s) 5, 18, 187
CviKI_1 RGCY 3 cut(s) 5, 18, 187
CviQI GTAC 1 cut(s) 41
DpnI GATC 3 cut(s) 114, 216, 300
DpnII GATC 3 cut(s) 112, 214, 298
DraIII CACNNNGTG 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 5, 262
EcoT22I ATGCAT 1 cut(s) 128
FaeI CATG 4 cut(s) 126, 194, 231, 359
FatI CATG 4 cut(s) 122, 190, 227, 355
FokI GGATG 1 cut(s) 113
FspBI CTAG 2 cut(s) 44, 56
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 174
Hin1II CATG 4 cut(s) 126, 194, 231, 359
HinfI GANTC 3 cut(s) 64, 118, 259
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 2 cut(s) 109, 286
Hpy188I TCNGA 3 cut(s) 117, 219, 243
Hpy188III TCNNGA 3 cut(s) 191, 205, 269
Hpy8I GTNNAC 2 cut(s) 109, 286
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4V TGCA 6 cut(s) 14, 126, 197, 227, 237, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
Hsp92II CATG 4 cut(s) 126, 194, 231, 359
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 3 cut(s) 112, 214, 298
LmnI GCTCC 1 cut(s) 150
LpnPI CCDG 6 cut(s) 19, 86, 171, 187, 249, 276
LweI GCATC 3 cut(s) 92, 135, 184
MaeI CTAG 2 cut(s) 44, 56
MaeIII GTNAC 4 cut(s) 25, 58, 88, 202
MalI GATC 3 cut(s) 114, 216, 300
MboI GATC 3 cut(s) 112, 214, 298
MflI RGATCY 1 cut(s) 298
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 309, 313
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 58, 112
MnlI CCTC 4 cut(s) 140, 154, 205, 328
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 128
MroNI GCCGGC 1 cut(s) 173
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 78, 312
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 7, 264
Mva1269I GAATGC 1 cut(s) 237
MvaI CCWGG 2 cut(s) 7, 264
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 175
NdeII GATC 3 cut(s) 112, 214, 298
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 4 cut(s) 126, 194, 231, 359
NmuCI GTSAC 3 cut(s) 25, 58, 202
NsiI ATGCAT 1 cut(s) 128
NspI RCATGY 1 cut(s) 231
PaeI GCATGC 1 cut(s) 231
PagI TCATGA 1 cut(s) 190
PctI GAATGC 1 cut(s) 237
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 259
PleI GAGTC 2 cut(s) 58, 112
PpsI GAGTC 2 cut(s) 58, 112
PshBI ATTAAT 1 cut(s) 312
PsiI TTATAA 1 cut(s) 326
Psp6I CCWGG 2 cut(s) 5, 262
PspGI CCWGG 2 cut(s) 5, 262
PsuI RGATCY 1 cut(s) 298
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
SaqAI TTAA 2 cut(s) 78, 312
Sau3AI GATC 3 cut(s) 112, 214, 298
SchI GAGTC 2 cut(s) 58, 112
ScrFI CCNGG 2 cut(s) 7, 264
SetI ASST 2 cut(s) 11, 105
SfaNI GCATC 3 cut(s) 92, 135, 184
SpeI ACTAGT 1 cut(s) 43
SphI GCATGC 1 cut(s) 231
Sse9I AATT 2 cut(s) 309, 313
SsiI CCGC 1 cut(s) 231
SspMI CTAG 2 cut(s) 44, 56
StyD4I CCNGG 2 cut(s) 5, 262
TaaI ACNGT 1 cut(s) 281
TaqI TCGA 1 cut(s) 270
TasI AATT 2 cut(s) 309, 313
TfiI GAWTC 1 cut(s) 259
Tru1I TTAA 2 cut(s) 78, 312
Tru9I TTAA 2 cut(s) 78, 312
TseFI GTSAC 3 cut(s) 25, 58, 202
Tsp45I GTSAC 3 cut(s) 25, 58, 202
TspDTI ATGAA 3 cut(s) 179, 344, 358
TspGWI ACGGA 1 cut(s) 57
VspI ATTAAT 1 cut(s) 312
XceI RCATGY 1 cut(s) 231
XspI CTAG 2 cut(s) 44, 56
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.