Rh5CG522200

Plant lipid transfer protein / seed storage protein / trypsin-alpha amylase inhibitor domain family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0003691
Physical Location & Seq
Forward (+)
73227796 .. 73228511
716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG522200.1

Sequence Viewer

Length: 282 bp
ATGGCCAGGTTTGCAGGCTATGGGGTGACATTCTTGGTGGTACTAGTATCTGTTCTAGTGACGACTCCGTATGTCATTAAGGCATCTGTAACTTGCGAGCAGGTCGTAAACTATCTGACTCCATGCATCCCTTTTGGAGTGTTTGGAGGGAGCGTGCCAGAGGAGTGTTGTGCCGGCGTAAAAGGGCTTCATGATGCAGAAGTCGATCACGACAGAGGATCGGAGAACTGCATGCGGTTGCATTCAGAAAGGGTGTGCAATGATTCCTGGTCTCGACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

93

Amino Acids

10.14

Weight (kDa)

5.45

Isoelectric Point (pI)

28.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 91
AciI CCGC 1 cut(s) 235
AclWI GGATC 1 cut(s) 226
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 42
AhlI ACTAGT 1 cut(s) 43
AjnI CCWGG 2 cut(s) 5, 266
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 1 cut(s) 226
AoxI GGCC 1 cut(s) 3
AsuHPI GGTGA 1 cut(s) 37
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 171, 203
BciT130I CCWGG 2 cut(s) 7, 268
BcoDI GTCTC 1 cut(s) 276
BcuI ACTAGT 1 cut(s) 43
BfaI CTAG 2 cut(s) 44, 56
BfuAI ACCTGC 1 cut(s) 91
Bme1390I CCNGG 2 cut(s) 7, 268
BmrFI CCNGG 2 cut(s) 7, 268
BmsI GCATC 3 cut(s) 92, 135, 184
BsaI GGTCTC 1 cut(s) 276
BsaXI ACNNNNNCTCC 2 cut(s) 138, 168
Bse118I RCCGGY 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 265
BseBI CCWGG 2 cut(s) 7, 268
BseGI GGATG 1 cut(s) 126
BseMI GCAATG 1 cut(s) 265
BseRI GAGGAG 1 cut(s) 176
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 174
BsmAI GTCTC 1 cut(s) 276
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 276
Bsp143I GATC 2 cut(s) 205, 218
BspACI CCGC 1 cut(s) 235
BspANI GGCC 1 cut(s) 5
BspHI TCATGA 1 cut(s) 190
BspMI ACCTGC 1 cut(s) 91
BspPI GGATC 1 cut(s) 226
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 265
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssMI GATC 2 cut(s) 205, 218
Bst2UI CCWGG 2 cut(s) 7, 268
BstC8I GCNNGC 5 cut(s) 16, 98, 155, 175, 233
BstF5I GGATG 1 cut(s) 126
BstKTI GATC 2 cut(s) 208, 221
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 2 cut(s) 205, 218
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 7, 268
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 2 cut(s) 5, 266
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 91
Cac8I GCNNGC 5 cut(s) 16, 98, 155, 175, 233
CciI TCATGA 1 cut(s) 190
Cfr10I RCCGGY 1 cut(s) 173
Csp6I GTAC 1 cut(s) 41
CviAII CATG 3 cut(s) 123, 191, 232
CviJI RGCY 3 cut(s) 5, 18, 187
CviKI_1 RGCY 3 cut(s) 5, 18, 187
CviQI GTAC 1 cut(s) 41
DpnI GATC 2 cut(s) 207, 220
DpnII GATC 2 cut(s) 205, 218
EaeI YGGCCR 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 276
EcoRII CCWGG 2 cut(s) 5, 266
EcoT22I ATGCAT 1 cut(s) 128
FaeI CATG 3 cut(s) 126, 194, 235
FaiI YATR 6 cut(s) 21, 72, 124, 192, 233, 280
FatI CATG 3 cut(s) 122, 190, 231
FokI GGATG 1 cut(s) 113
FspBI CTAG 2 cut(s) 44, 56
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 174
Hin1II CATG 3 cut(s) 126, 194, 235
HinfI GANTC 3 cut(s) 64, 118, 263
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 3 cut(s) 117, 223, 247
Hpy188III TCNNGA 3 cut(s) 191, 209, 273
Hpy8I GTNNAC 1 cut(s) 109
HpyCH4V TGCA 6 cut(s) 14, 126, 197, 231, 241, 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
Hsp92II CATG 3 cut(s) 126, 194, 235
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 205, 218
LmnI GCTCC 1 cut(s) 150
LpnPI CCDG 5 cut(s) 19, 86, 171, 187, 253
LweI GCATC 3 cut(s) 92, 135, 184
MaeI CTAG 2 cut(s) 44, 56
MaeIII GTNAC 3 cut(s) 25, 58, 88
MalI GATC 2 cut(s) 207, 220
MboI GATC 2 cut(s) 205, 218
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 58, 112
MnlI CCTC 3 cut(s) 140, 154, 209
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 128
MroNI GCCGGC 1 cut(s) 173
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 78
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 2 cut(s) 7, 268
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 2 cut(s) 7, 268
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 175
NdeII GATC 2 cut(s) 205, 218
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 3 cut(s) 126, 194, 235
NmuCI GTSAC 2 cut(s) 25, 58
NsiI ATGCAT 1 cut(s) 128
NspI RCATGY 1 cut(s) 235
PaeI GCATGC 1 cut(s) 235
PagI TCATGA 1 cut(s) 190
PctI GAATGC 1 cut(s) 241
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 263
PleI GAGTC 2 cut(s) 58, 112
PpsI GAGTC 2 cut(s) 58, 112
Psp6I CCWGG 2 cut(s) 5, 266
PspGI CCWGG 2 cut(s) 5, 266
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 78
Sau3AI GATC 2 cut(s) 205, 218
SchI GAGTC 2 cut(s) 58, 112
ScrFI CCNGG 2 cut(s) 7, 268
SetI ASST 2 cut(s) 11, 105
SfaNI GCATC 3 cut(s) 92, 135, 184
SpeI ACTAGT 1 cut(s) 43
SphI GCATGC 1 cut(s) 235
SsiI CCGC 1 cut(s) 235
SspMI CTAG 2 cut(s) 44, 56
StyD4I CCNGG 2 cut(s) 5, 266
TaqI TCGA 2 cut(s) 204, 274
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseFI GTSAC 2 cut(s) 25, 58
Tsp45I GTSAC 2 cut(s) 25, 58
TspDTI ATGAA 1 cut(s) 179
TspGWI ACGGA 1 cut(s) 57
XceI RCATGY 1 cut(s) 235
XspI CTAG 2 cut(s) 44, 56
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.