MD03G1052000.v1.1

Non-specific lipid-transfer protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003691
Physical Location & Seq
Reverse (-)
4145699 .. 4146040
342 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1052000.v1.1.491

Sequence Viewer

Length: 342 bp
ATGCCCAGGTTTTCAGTCATCGTGGCAATAGTATTCTTATTGGTGACAGCTCCTTCTGTCACTAATGCCACCATAACTTGTGGTGAAGTGGTAGTTTTGCTCACTCCATGCATACCCTTTGGAGTGTTTGGAGGCACAGTGCCTCCAGATTGCTGCAAAGGCATAAAAGGGCTGAATGCTGCAAAAAAGACGACGATGGACCGGAGAATTGCTTGCAGTTGCATTCAGGAAGGAGCTGCAAGAATCCCCGGGATTAATTATGACCGGATTAACACTCTTTGTGATGTTTGCGACTCTCCTTGTCCCTACAAAGTTTACCCCTCCACTAATTGCTCTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tryp_alpha_amyl

Protein Analysis

114

Amino Acids

11.95

Weight (kDa)

8.36

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tryp_alpha_amyl PF00234 27 - 111 1.2e-06 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AjnI CCWGG 1 cut(s) 5
AluBI AGCT 2 cut(s) 50, 236
AluI AGCT 2 cut(s) 50, 236
Ama87I CYCGRG 1 cut(s) 248
ApeKI GCWGC 3 cut(s) 153, 179, 236
AseI ATTAAT 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 2 cut(s) 249, 250
AsuHPI GGTGA 2 cut(s) 55, 95
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 199
BbvI GCAGC 3 cut(s) 140, 166, 223
BccI CCATC 1 cut(s) 190
BciT130I CCWGG 1 cut(s) 7
BcnI CCSGG 2 cut(s) 249, 250
BisI GCNGC 3 cut(s) 154, 180, 237
BlsI GCNGC 3 cut(s) 155, 181, 238
Bme1390I CCNGG 3 cut(s) 7, 249, 250
Bme18I GGWCC 1 cut(s) 199
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 199
BmrFI CCNGG 3 cut(s) 7, 249, 250
BpmI CTGGAG 1 cut(s) 129
BpuMI CCSGG 2 cut(s) 249, 250
BsaJI CCNNGG 3 cut(s) 5, 247, 248
BsaWI WCCGGW 2 cut(s) 201, 264
BsaXI ACNNNNNCTCC 4 cut(s) 123, 127, 153, 157
BseBI CCWGG 1 cut(s) 7
BseDI CCNNGG 3 cut(s) 5, 247, 248
BseXI GCAGC 3 cut(s) 140, 166, 223
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 3 cut(s) 202, 249, 265
BslFI GGGAC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 288
BsmI GAATGC 2 cut(s) 181, 222
BsoBI CYCGRG 1 cut(s) 248
BssECI CCNNGG 3 cut(s) 5, 247, 248
Bst2UI CCWGG 1 cut(s) 7
Bst4CI ACNGT 1 cut(s) 139
BstC8I GCNNGC 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 1 cut(s) 7
BstSCI CCNGG 3 cut(s) 5, 247, 248
BstV1I GCAGC 3 cut(s) 140, 166, 223
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 214
Cfr13I GGNCC 1 cut(s) 199
Cfr9I CCCGGG 1 cut(s) 248
CviAII CATG 1 cut(s) 108
CviJI RGCY 3 cut(s) 50, 172, 236
CviKI_1 RGCY 3 cut(s) 50, 172, 236
Eco47I GGWCC 1 cut(s) 199
Eco88I CYCGRG 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 5
EcoT22I ATGCAT 1 cut(s) 113
FaeI CATG 1 cut(s) 111
FaiI YATR 5 cut(s) 74, 109, 113, 164, 261
FaqI GGGAC 1 cut(s) 288
FatI CATG 1 cut(s) 107
Fnu4HI GCNGC 3 cut(s) 154, 180, 237
Fsp4HI GCNGC 3 cut(s) 154, 180, 237
GluI GCNGC 3 cut(s) 154, 180, 237
GsuI CTGGAG 1 cut(s) 129
HapII CCGG 3 cut(s) 202, 249, 265
Hin1II CATG 1 cut(s) 111
HinfI GANTC 2 cut(s) 243, 293
HpaII CCGG 3 cut(s) 202, 249, 265
HphI GGTGA 2 cut(s) 55, 95
Hpy166II GTNNAC 1 cut(s) 316
Hpy188III TCNNGA 2 cut(s) 146, 227
Hpy8I GTNNAC 1 cut(s) 316
Hpy99I CGWCG 1 cut(s) 196
HpyAV CCTTC 2 cut(s) 63, 224
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 6 cut(s) 111, 156, 182, 216, 222, 239
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
Hsp92II CATG 1 cut(s) 111
LmnI GCTCC 2 cut(s) 55, 233
LpnPI CCDG 6 cut(s) 19, 159, 212, 215, 262, 278
Lsp1109I GCAGC 3 cut(s) 140, 166, 223
MaeIII GTNAC 2 cut(s) 43, 58
MluCI AATT 3 cut(s) 207, 256, 328
MlyI GAGTC 1 cut(s) 287
MnlI CCTC 3 cut(s) 125, 153, 331
Mph1103I ATGCAT 1 cut(s) 113
MseI TTAA 2 cut(s) 255, 270
MspI CCGG 3 cut(s) 202, 249, 265
MspR9I CCNGG 3 cut(s) 7, 249, 250
Mva1269I GAATGC 2 cut(s) 181, 222
MvaI CCWGG 1 cut(s) 7
MwoI GCNNNNNNNGC 1 cut(s) 159
NciI CCSGG 2 cut(s) 249, 250
NlaIII CATG 1 cut(s) 111
NmuCI GTSAC 2 cut(s) 43, 58
NsiI ATGCAT 1 cut(s) 113
PctI GAATGC 2 cut(s) 181, 222
PfeI GAWTC 1 cut(s) 243
PkrI GCNGC 3 cut(s) 155, 181, 238
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
PshBI ATTAAT 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 5
PspGI CCWGG 1 cut(s) 5
PspPI GGNCC 1 cut(s) 199
SaqAI TTAA 2 cut(s) 255, 270
SatI GCNGC 3 cut(s) 154, 180, 237
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 3 cut(s) 7, 249, 250
SetI ASST 3 cut(s) 11, 52, 238
SinI GGWCC 1 cut(s) 199
SmaI CCCGGG 1 cut(s) 250
Sse9I AATT 3 cut(s) 207, 256, 328
StyD4I CCNGG 3 cut(s) 5, 247, 248
TaaI ACNGT 1 cut(s) 139
TasI AATT 3 cut(s) 207, 256, 328
TfiI GAWTC 1 cut(s) 243
Tru1I TTAA 2 cut(s) 255, 270
Tru9I TTAA 2 cut(s) 255, 270
TscAI CASTG 1 cut(s) 144
TseFI GTSAC 2 cut(s) 43, 58
TseI GCWGC 3 cut(s) 153, 179, 236
Tsp45I GTSAC 2 cut(s) 43, 58
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 1 cut(s) 144
VpaK11BI GGWCC 1 cut(s) 199
VspI ATTAAT 1 cut(s) 255
XmaI CCCGGG 1 cut(s) 248
Zsp2I ATGCAT 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.