FvH4_3g40510

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001899
Physical Location & Seq
Forward (+)
34171810 .. 34174068
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40510.t2

Sequence Viewer

Length: 624 bp
ATGACCATCAGCATAGTAGACCACGAATTACCATATGACACCCACAATCTCTATGATGTATCGTTTTACGATGATCAAATCCACACCCTAGTCACTCACAGCGCACCCATGGTGGAAGCCTGGCTCTCTGAAATCATCCCCATAGTCAATCAAAACCCAATTGTAGGCCTGGACGTGGAGTGGCGTCCAAATAACCGCAACAGCGACAACCTCGTTGCGACATTGCAGCTTTGTGTGGACCATCGTTGCCTCATCTTCCAACTTCTTTACGCCCAAACCATCCCGCGAGCACTCTTTGACTTTCTCAGTAATGGCAGTTACACATTTGTGGGTGCTGGGATCGAGAATGATATGGAGAAGCTCCTTGTGGACTATGACCTGAAGGTGACCAATACTCTTGATCTCGGGCCTTTGGCAGCAACTAAATTGGGTAAGATTGAATTGAAAAAAGCTGGCTTGAAGGGTCTGTCTAAAGAGGTCCTTGGGAAGGAGCTCCAGAAGCCTAGCAATGTGACAAGGAGCAGGTGGGACAACGAGTGGCTTATGTGTAATCAGGTTCAGTACGCTTGTATTGATGCTTTTATTTCCTCAGAGATTGGAAGGCGGTTGACTGCCATGGCTTGA

Protein Analysis

208

Amino Acids

23.38

Weight (kDa)

5.06

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 42 - 204 1.9e-16 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 513
Acc36I ACCTGC 1 cut(s) 513
AccI GTMKAC 1 cut(s) 18
AccII CGCG 1 cut(s) 286
AciI CCGC 3 cut(s) 196, 284, 604
AclWI GGATC 1 cut(s) 347
AcuI CTGAAG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 184
AfaI GTAC 1 cut(s) 563
AfiI CCNNNNNNNGG 3 cut(s) 164, 175, 487
AgsI TTSAA 3 cut(s) 440, 445, 460
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 2 cut(s) 119, 168
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 4 cut(s) 229, 361, 452, 493
AluI AGCT 4 cut(s) 229, 361, 452, 493
Alw21I GWGCWC 2 cut(s) 292, 495
AlwI GGATC 1 cut(s) 347
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 2 cut(s) 166, 407
ApeKI GCWGC 2 cut(s) 226, 416
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 3 cut(s) 238, 407, 478
AsuHPI GGTGA 1 cut(s) 397
AvaI CYCGRG 1 cut(s) 404
AvaII GGWCC 2 cut(s) 238, 478
BanII GRGCYC 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 292, 495
BbvI GCAGC 2 cut(s) 238, 428
BccI CCATC 3 cut(s) 14, 249, 287
BciT130I CCWGG 2 cut(s) 121, 170
BclI TGATCA 1 cut(s) 73
BfaI CTAG 2 cut(s) 89, 504
BfuAI ACCTGC 1 cut(s) 513
BisI GCNGC 2 cut(s) 227, 417
BlsI GCNGC 2 cut(s) 228, 418
Bme1390I CCNGG 2 cut(s) 121, 170
Bme18I GGWCC 2 cut(s) 238, 478
BmeT110I CYCGRG 1 cut(s) 404
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 3 cut(s) 238, 407, 478
BmrFI CCNGG 2 cut(s) 121, 170
BmsI GCATC 1 cut(s) 565
BpmI CTGGAG 1 cut(s) 479
BsaHI GRCGYC 1 cut(s) 184
BsaJI CCNNGG 3 cut(s) 108, 481, 615
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 175, 487
Bse3DI GCAATG 2 cut(s) 221, 514
BseBI CCWGG 2 cut(s) 121, 170
BseDI CCNNGG 3 cut(s) 108, 481, 615
BseGI GGATG 2 cut(s) 135, 279
BseLI CCNNNNNNNGG 3 cut(s) 164, 175, 487
BseMI GCAATG 2 cut(s) 221, 514
BseMII CTCAG 2 cut(s) 319, 603
BseXI GCAGC 2 cut(s) 238, 428
BseYI CCCAGC 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 286
BshFI GGCC 2 cut(s) 168, 409
BsiHKAI GWGCWC 2 cut(s) 292, 495
BsiHKCI CYCGRG 1 cut(s) 404
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 3 cut(s) 164, 175, 487
BsmFI GGGAC 1 cut(s) 542
BsnI GGCC 2 cut(s) 168, 409
BsoBI CYCGRG 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 292, 495
Bsp143I GATC 3 cut(s) 73, 339, 400
Bsp19I CCATGG 2 cut(s) 108, 615
BspACI CCGC 3 cut(s) 196, 284, 604
BspANI GGCC 2 cut(s) 168, 409
BspCNI CTCAG 2 cut(s) 318, 602
BspFNI CGCG 1 cut(s) 286
BspMI ACCTGC 1 cut(s) 513
BspPI GGATC 1 cut(s) 347
BsrDI GCAATG 2 cut(s) 221, 514
BssECI CCNNGG 3 cut(s) 108, 481, 615
BssMI GATC 3 cut(s) 73, 339, 400
BssNI GRCGYC 1 cut(s) 184
BssT1I CCWWGG 3 cut(s) 108, 481, 615
Bst2UI CCWGG 2 cut(s) 121, 170
BstACI GRCGYC 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 288, 454
BstDEI CTNAG 2 cut(s) 305, 589
BstDSI CCRYGG 2 cut(s) 108, 615
BstEII GGTNACC 1 cut(s) 385
BstENI CCTNNNNNAGG 1 cut(s) 485
BstF5I GGATG 2 cut(s) 135, 279
BstFNI CGCG 1 cut(s) 286
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 3 cut(s) 76, 342, 403
BstMBI GATC 3 cut(s) 73, 339, 400
BstMWI GCNNNNNNNGC 1 cut(s) 499
BstNI CCWGG 2 cut(s) 121, 170
BstPI GGTNACC 1 cut(s) 385
BstSCI CCNGG 2 cut(s) 119, 168
BstUI CGCG 1 cut(s) 286
BstV1I GCAGC 2 cut(s) 238, 428
BsuRI GGCC 2 cut(s) 168, 409
BtgI CCRYGG 2 cut(s) 108, 615
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 2 cut(s) 135, 279
BveI ACCTGC 1 cut(s) 513
Cac8I GCNNGC 2 cut(s) 288, 454
CfoI GCGC 1 cut(s) 104
Cfr13I GGNCC 3 cut(s) 238, 407, 478
CseI GACGC 1 cut(s) 173
Csp6I GTAC 1 cut(s) 562
CviAII CATG 2 cut(s) 109, 616
CviQI GTAC 1 cut(s) 562
DdeI CTNAG 2 cut(s) 305, 589
DpnI GATC 3 cut(s) 75, 341, 402
DpnII GATC 3 cut(s) 73, 339, 400
Ecl136II GAGCTC 1 cut(s) 493
Eco130I CCWWGG 3 cut(s) 108, 481, 615
Eco147I AGGCCT 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 495
Eco47I GGWCC 2 cut(s) 238, 478
Eco53kI GAGCTC 1 cut(s) 493
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 404
Eco91I GGTNACC 1 cut(s) 385
EcoICRI GAGCTC 1 cut(s) 493
EcoNI CCTNNNNNAGG 1 cut(s) 485
EcoO109I RGGNCCY 1 cut(s) 478
EcoO65I GGTNACC 1 cut(s) 385
EcoRII CCWGG 2 cut(s) 119, 168
EcoT14I CCWWGG 3 cut(s) 108, 481, 615
EcoT38I GRGCYC 1 cut(s) 495
ErhI CCWWGG 3 cut(s) 108, 481, 615
FaeI CATG 2 cut(s) 112, 619
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 1 cut(s) 542
FatI CATG 2 cut(s) 108, 615
FauI CCCGC 1 cut(s) 291
FauNDI CATATG 1 cut(s) 34
FbaI TGATCA 1 cut(s) 73
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 2 cut(s) 227, 417
FokI GGATG 2 cut(s) 122, 266
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 2 cut(s) 227, 417
FspBI CTAG 2 cut(s) 89, 504
GlaI GCGC 1 cut(s) 103
GluI GCNGC 2 cut(s) 227, 417
GsaI CCCAGC 1 cut(s) 339
GsuI CTGGAG 1 cut(s) 479
HaeIII GGCC 2 cut(s) 168, 409
HgaI GACGC 1 cut(s) 173
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 1 cut(s) 184
Hin1II CATG 2 cut(s) 112, 619
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HphI GGTGA 1 cut(s) 397
Hpy166II GTNNAC 4 cut(s) 19, 238, 370, 609
Hpy188I TCNGA 2 cut(s) 130, 592
Hpy188III TCNNGA 3 cut(s) 343, 398, 496
Hpy8I GTNNAC 4 cut(s) 19, 238, 370, 609
HpyAV CCTTC 4 cut(s) 376, 454, 481, 594
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 1 cut(s) 226
HpyF10VI GCNNNNNNNGC 1 cut(s) 499
HpyF3I CTNAG 2 cut(s) 305, 589
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 184
Hsp92II CATG 2 cut(s) 112, 619
HspAI GCGC 1 cut(s) 102
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 3 cut(s) 73, 339, 400
LmnI GCTCC 4 cut(s) 366, 490, 498, 519
Lsp1109I GCAGC 2 cut(s) 238, 428
LweI GCATC 1 cut(s) 565
MaeI CTAG 2 cut(s) 89, 504
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 4 cut(s) 91, 317, 385, 511
MalI GATC 3 cut(s) 75, 341, 402
MboI GATC 3 cut(s) 73, 339, 400
MboII GAAGA 1 cut(s) 247
MfeI CAATTG 1 cut(s) 159
MhlI GDGCHC 2 cut(s) 292, 495
MluCI AATT 4 cut(s) 26, 159, 425, 440
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 4 cut(s) 221, 260, 469, 598
MslI CAYNNNNRTG 1 cut(s) 326
MspR9I CCNGG 2 cut(s) 121, 170
MunI CAATTG 1 cut(s) 159
MvaI CCWGG 2 cut(s) 121, 170
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 1 cut(s) 499
NcoI CCATGG 2 cut(s) 108, 615
NdeI CATATG 1 cut(s) 34
NdeII GATC 3 cut(s) 73, 339, 400
NlaIII CATG 2 cut(s) 112, 619
NmuCI GTSAC 3 cut(s) 91, 385, 511
OliI CACNNNNGTG 1 cut(s) 326
PaqCI CACCTGC 1 cut(s) 513
PceI AGGCCT 1 cut(s) 168
PkrI GCNGC 2 cut(s) 228, 418
PpuMI RGGWCCY 1 cut(s) 478
Psp124BI GAGCTC 1 cut(s) 495
Psp5II RGGWCCY 1 cut(s) 478
Psp6I CCWGG 2 cut(s) 119, 168
PspEI GGTNACC 1 cut(s) 385
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 2 cut(s) 119, 168
PspPI GGNCC 3 cut(s) 238, 407, 478
PspPPI RGGWCCY 1 cut(s) 478
RsaI GTAC 1 cut(s) 563
RsaNI GTAC 1 cut(s) 562
RseI CAYNNNNRTG 1 cut(s) 326
SacI GAGCTC 1 cut(s) 495
SatI GCNGC 2 cut(s) 227, 417
Sau3AI GATC 3 cut(s) 73, 339, 400
Sau96I GGNCC 3 cut(s) 238, 407, 478
ScrFI CCNGG 2 cut(s) 121, 170
SduI GDGCHC 2 cut(s) 292, 495
SfaNI GCATC 1 cut(s) 565
SinI GGWCC 2 cut(s) 238, 478
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 4 cut(s) 26, 159, 425, 440
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 3 cut(s) 196, 284, 604
SspMI CTAG 2 cut(s) 89, 504
SstI GAGCTC 1 cut(s) 495
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 119, 168
StyI CCWWGG 3 cut(s) 108, 481, 615
TaiI ACGT 1 cut(s) 177
TaqI TCGA 1 cut(s) 342
TasI AATT 4 cut(s) 26, 159, 425, 440
TseFI GTSAC 3 cut(s) 91, 385, 511
TseI GCWGC 2 cut(s) 226, 416
Tsp45I GTSAC 3 cut(s) 91, 385, 511
VpaK11BI GGWCC 2 cut(s) 238, 478
XagI CCTNNNNNAGG 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 18
XspI CTAG 2 cut(s) 89, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.