Rw4G037430

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001899
Physical Location & Seq
Reverse (-)
64045816 .. 64046517
702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G037430.1

Sequence Viewer

Length: 702 bp
ATGGCGGATTCGGATCACAAGTTGTATCATATTGAATTCTATGAAGACTGTATCACGACTACCGTAACCGGAAGCCCCCATATCGTCGATCAGTGGGTTAACGAAACTTATTGCTTGCATGGCAGTGGCAGCCATGAGATGAACTTGTTTGTAGGGCTCGACACCGAATGGCGCTTCGACCCTGAAGATCATGATTACCAAGTCGCAATCATTCAACTCTGTGACGTTCGTCGCCGTTGCCTCATCTTCCAGTTTTCTCAGCAAACCCAAACCGGCGAGATAATTAACAATGCTCCTCCTCATTCTCTCACCGCTTTTCTGGCCGACCAAAACATCAGCTTTGTTGGCAAGCAAGTTAAGGAGGATGTTAAACGTTTGGCTCGTTTGGGATTGCATGTTTCACGTGCTGTGGATGTGTCTCATATGGCCGCCGAAAAGTACAGAGACAAAAGCCACAGATGCAAGGGACTTAAAACCTTGGCCATGGAGTTCCTGAACAAGGAGATGAACAAGCATAAGGATATCACGCTGAGCCGTTGGGACGTTGAGCAACTAAGTCTTGCCCAAGTCGAATATGCTTGCATTGATGCCTTCGTTTCGCTTCTACTCGGAATCCTACTCAGTACTAGTAATGATCAGCTGGATCATCATCATCCCATCAATAACATAAAACAAAGTCAGCATTCTGCCCTGTCTAGCTAG

Protein Analysis

233

Amino Acids

26.65

Weight (kDa)

5.88

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 50 - 201 3.5e-16 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 312, 429
AclI AACGTT 1 cut(s) 373
AclWI GGATC 2 cut(s) 21, 651
AcoI YGGCCR 3 cut(s) 321, 426, 480
AcsI RAATTY 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 204
AcvI CACGTG 1 cut(s) 404
AfaI GTAC 2 cut(s) 440, 625
AfiI CCNNNNNNNGG 1 cut(s) 499
AgsI TTSAA 2 cut(s) 35, 215
AhlI ACTAGT 1 cut(s) 626
AluBI AGCT 3 cut(s) 339, 640, 699
AluI AGCT 3 cut(s) 339, 640, 699
Alw26I GTCTC 2 cut(s) 423, 438
AlwI GGATC 2 cut(s) 21, 651
AoxI GGCC 3 cut(s) 321, 426, 480
ApeKI GCWGC 1 cut(s) 129
ApoI RAATTY 1 cut(s) 35
AspLEI GCGC 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 301
BalI TGGCCA 1 cut(s) 482
BanII GRGCYC 1 cut(s) 159
BbrPI CACGTG 1 cut(s) 404
BbsI GAAGAC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 141
BccI CCATC 1 cut(s) 665
BceAI ACGGC 2 cut(s) 219, 519
BclI TGATCA 1 cut(s) 634
BcoDI GTCTC 2 cut(s) 423, 438
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 3 cut(s) 627, 696, 700
BfoI RGCGCY 1 cut(s) 175
BisI GCNGC 2 cut(s) 130, 429
BlpI GCTNAGC 1 cut(s) 530
BlsI GCNGC 2 cut(s) 131, 430
BmcAI AGTACT 1 cut(s) 625
BmsI GCATC 2 cut(s) 449, 577
BoxI GACNNNNGTC 1 cut(s) 228
BpiI GAAGAC 1 cut(s) 51
Bpu1102I GCTNAGC 1 cut(s) 530
BsaAI YACGTR 1 cut(s) 404
BsaBI GATNNNNATC 2 cut(s) 12, 648
BsaJI CCNNGG 2 cut(s) 477, 483
BsaWI WCCGGW 1 cut(s) 68
Bsc4I CCNNNNNNNGG 1 cut(s) 499
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 250
Bse8I GATNNNNATC 2 cut(s) 12, 648
BseDI CCNNGG 2 cut(s) 477, 483
BseGI GGATG 3 cut(s) 370, 418, 652
BseJI GATNNNNATC 2 cut(s) 12, 648
BseLI CCNNNNNNNGG 1 cut(s) 499
BseMII CTCAG 3 cut(s) 272, 521, 634
BseNI ACTGG 1 cut(s) 250
BseRI GAGGAG 2 cut(s) 285, 288
BseXI GCAGC 1 cut(s) 141
BshFI GGCC 3 cut(s) 323, 428, 482
BsiSI CCGG 2 cut(s) 69, 273
BslFI GGGAC 2 cut(s) 480, 554
BslI CCNNNNNNNGG 1 cut(s) 499
BsmAI GTCTC 2 cut(s) 423, 438
BsmFI GGGAC 2 cut(s) 480, 554
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 3 cut(s) 323, 428, 482
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 5 cut(s) 13, 88, 187, 634, 643
Bsp1720I GCTNAGC 1 cut(s) 530
Bsp19I CCATGG 1 cut(s) 483
BspACI CCGC 3 cut(s) 5, 312, 429
BspANI GGCC 3 cut(s) 323, 428, 482
BspCNI CTCAG 3 cut(s) 271, 522, 633
BspHI TCATGA 1 cut(s) 190
BspPI GGATC 2 cut(s) 21, 651
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 2 cut(s) 477, 483
BssMI GATC 5 cut(s) 13, 88, 187, 634, 643
BssT1I CCWWGG 2 cut(s) 477, 483
Bst4CI ACNGT 2 cut(s) 50, 64
BstBAI YACGTR 1 cut(s) 404
BstC8I GCNNGC 3 cut(s) 116, 350, 580
BstDEI CTNAG 4 cut(s) 258, 530, 554, 620
BstDSI CCRYGG 1 cut(s) 483
BstENI CCTNNNNNAGG 1 cut(s) 497
BstF5I GGATG 3 cut(s) 370, 418, 652
BstH2I RGCGCY 1 cut(s) 175
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 5 cut(s) 16, 91, 190, 637, 646
BstMAI GTCTC 2 cut(s) 423, 438
BstMBI GATC 5 cut(s) 13, 88, 187, 634, 643
BstMWI GCNNNNNNNGC 5 cut(s) 120, 129, 320, 345, 459
BstNSI RCATGY 1 cut(s) 398
BstPAI GACNNNNGTC 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 141
BstV2I GAAGAC 1 cut(s) 51
BsuRI GGCC 3 cut(s) 323, 428, 482
BtgI CCRYGG 1 cut(s) 483
BtsCI GGATG 3 cut(s) 370, 418, 652
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 2 cut(s) 98, 130
Cac8I GCNNGC 3 cut(s) 116, 350, 580
CciI TCATGA 1 cut(s) 190
CfoI GCGC 1 cut(s) 174
Cfr10I RCCGGY 1 cut(s) 272
Csp6I GTAC 2 cut(s) 439, 624
CviAII CATG 5 cut(s) 119, 134, 191, 395, 484
CviQI GTAC 2 cut(s) 439, 624
DdeI CTNAG 4 cut(s) 258, 530, 554, 620
DpnI GATC 5 cut(s) 15, 90, 189, 636, 645
DpnII GATC 5 cut(s) 13, 88, 187, 634, 643
EaeI YGGCCR 3 cut(s) 321, 426, 480
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 477, 483
Eco24I GRGCYC 1 cut(s) 159
Eco32I GATATC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 204
Eco72I CACGTG 1 cut(s) 404
EcoNI CCTNNNNNAGG 1 cut(s) 497
EcoRI GAATTC 1 cut(s) 35
EcoRV GATATC 1 cut(s) 523
EcoT14I CCWWGG 2 cut(s) 477, 483
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 2 cut(s) 477, 483
FaeI CATG 5 cut(s) 122, 137, 194, 398, 487
FaqI GGGAC 2 cut(s) 480, 554
FatI CATG 5 cut(s) 118, 133, 190, 394, 483
FauNDI CATATG 1 cut(s) 423
FbaI TGATCA 1 cut(s) 634
Fnu4HI GCNGC 2 cut(s) 130, 429
FokI GGATG 3 cut(s) 377, 425, 639
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 2 cut(s) 130, 429
FspBI CTAG 3 cut(s) 627, 696, 700
GlaI GCGC 1 cut(s) 173
GluI GCNGC 2 cut(s) 130, 429
HaeII RGCGCY 1 cut(s) 175
HaeIII GGCC 3 cut(s) 323, 428, 482
HapII CCGG 2 cut(s) 69, 273
HhaI GCGC 1 cut(s) 174
Hin1II CATG 5 cut(s) 122, 137, 194, 398, 487
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 2 cut(s) 8, 612
HpaI GTTAAC 1 cut(s) 100
HpaII CCGG 2 cut(s) 69, 273
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 2 cut(s) 13, 611
Hpy188III TCNNGA 3 cut(s) 55, 191, 493
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 2 cut(s) 89, 234
HpyAV CCTTC 1 cut(s) 601
HpyCH4III ACNGT 2 cut(s) 50, 64
HpyCH4IV ACGT 4 cut(s) 225, 373, 403, 543
HpyCH4V TGCA 4 cut(s) 118, 394, 462, 582
HpyF10VI GCNNNNNNNGC 5 cut(s) 120, 129, 320, 345, 459
HpyF3I CTNAG 4 cut(s) 258, 530, 554, 620
HpySE526I ACGT 4 cut(s) 225, 373, 403, 543
Hsp92II CATG 5 cut(s) 122, 137, 194, 398, 487
HspAI GCGC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 634
KspAI GTTAAC 1 cut(s) 100
Kzo9I GATC 5 cut(s) 13, 88, 187, 634, 643
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 7 cut(s) 82, 195, 263, 286, 305, 506, 626
Lsp1109I GCAGC 1 cut(s) 141
LweI GCATC 2 cut(s) 449, 577
MaeI CTAG 3 cut(s) 627, 696, 700
MaeII ACGT 4 cut(s) 225, 373, 403, 543
MaeIII GTNAC 2 cut(s) 64, 221
MalI GATC 5 cut(s) 15, 90, 189, 636, 645
MboI GATC 5 cut(s) 13, 88, 187, 634, 643
MboII GAAGA 3 cut(s) 56, 197, 238
MhlI GDGCHC 1 cut(s) 159
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 2 cut(s) 35, 282
MluNI TGGCCA 1 cut(s) 482
MnlI CCTC 4 cut(s) 251, 306, 309, 355
Mox20I TGGCCA 1 cut(s) 482
MscI TGGCCA 1 cut(s) 482
MseI TTAA 5 cut(s) 99, 285, 357, 369, 471
MslI CAYNNNNRTG 1 cut(s) 123
Msp20I TGGCCA 1 cut(s) 482
MspA1I CMGCKG 1 cut(s) 640
MspI CCGG 2 cut(s) 69, 273
Mva1269I GAATGC 1 cut(s) 682
MwoI GCNNNNNNNGC 5 cut(s) 120, 129, 320, 345, 459
NcoI CCATGG 1 cut(s) 483
NdeI CATATG 1 cut(s) 423
NdeII GATC 5 cut(s) 13, 88, 187, 634, 643
NlaIII CATG 5 cut(s) 122, 137, 194, 398, 487
NmuCI GTSAC 1 cut(s) 221
NspI RCATGY 1 cut(s) 398
PagI TCATGA 1 cut(s) 190
PcsI WCGNNNNNNNCGW 1 cut(s) 379
PctI GAATGC 1 cut(s) 682
PfeI GAWTC 2 cut(s) 8, 612
PkrI GCNGC 2 cut(s) 131, 430
PmaCI CACGTG 1 cut(s) 404
PmlI CACGTG 1 cut(s) 404
Ppu21I YACGTR 1 cut(s) 404
PshAI GACNNNNGTC 1 cut(s) 228
Psp1406I AACGTT 1 cut(s) 373
PspCI CACGTG 1 cut(s) 404
PvuII CAGCTG 1 cut(s) 640
RsaI GTAC 2 cut(s) 440, 625
RsaNI GTAC 2 cut(s) 439, 624
RseI CAYNNNNRTG 1 cut(s) 123
SaqAI TTAA 5 cut(s) 99, 285, 357, 369, 471
SatI GCNGC 2 cut(s) 130, 429
Sau3AI GATC 5 cut(s) 13, 88, 187, 634, 643
ScaI AGTACT 1 cut(s) 625
SduI GDGCHC 1 cut(s) 159
SetI ASST 8 cut(s) 228, 341, 376, 406, 479, 546, 642, 701
SfaNI GCATC 2 cut(s) 449, 577
SmiMI CAYNNNNRTG 1 cut(s) 123
SpeI ACTAGT 1 cut(s) 626
Sse9I AATT 2 cut(s) 35, 282
SsiI CCGC 3 cut(s) 5, 312, 429
SspMI CTAG 3 cut(s) 627, 696, 700
StyI CCWWGG 2 cut(s) 477, 483
TaaI ACNGT 2 cut(s) 50, 64
TaiI ACGT 4 cut(s) 228, 376, 406, 546
TaqI TCGA 4 cut(s) 87, 159, 177, 570
TasI AATT 2 cut(s) 35, 282
TatI WGTACW 2 cut(s) 438, 623
TauI GCSGC 1 cut(s) 431
TfiI GAWTC 2 cut(s) 8, 612
Tru1I TTAA 5 cut(s) 99, 285, 357, 369, 471
Tru9I TTAA 5 cut(s) 99, 285, 357, 369, 471
TscAI CASTG 2 cut(s) 98, 130
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 1 cut(s) 129
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 3 cut(s) 57, 155, 521
TspRI CASTG 2 cut(s) 98, 130
XagI CCTNNNNNAGG 1 cut(s) 497
XapI RAATTY 1 cut(s) 35
XceI RCATGY 1 cut(s) 398
XspI CTAG 3 cut(s) 627, 696, 700
ZrmI AGTACT 1 cut(s) 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.