RLG00000005619

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001899
Physical Location & Seq
Forward (+)
341919 .. 342719
801 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005619

Sequence Viewer

Length: 705 bp
ATGGCGGATTCGGATCACAGGTTGTATCATATTGAATTCTATGAAGACCGTATCGCGACTACCGTAACCGGAAGCCCCCATATCGTCGATCAGTGGATTAACGAAACTTATAATTACTTGCATGGCACTGGCAGCCATGAGATGAACTTGTTTGTAGGGCTCGACACCGAATGGCGCTTCGACCCTGAAGCTCATGATTACCAAGTCGCAATCATTCAACTCTGTGACGTTCGTCGCCGTTGCCTCATCTTCCAGTTTTCTCAGCAAACCCAAACCGGCGAGATAATTAACAATGCTCCTCCTCATTCTCTCACCACTTTTCTGGCCGACCAAAACATCAGCTTTGTTGGCAAGCAAGTTAAGGAGGATGTTAAACGTTTGGCTCGTTTGGGATTGCATGTTTCACGTGCTGTGGATGTGTCTCATTTGGCCGCCGAAAAGTACAGAGACAAAAGCCACAGATGCAAGGGACTTAAAACCTTGGCCATGGAGTTCCTGAACAAGGAGATGAACAAGCATAAGGATATCACGCTGAGCCGTTGGGACGCTGAGCAACTAAGTCTTGCCCAAATCGAATATGCTTGCATTGATGCCTTCGTTTCGCTTCGACTCGGAATCCTACTCAGTACTACAAATGATCAGCTGGATCATCATCATCCCATCAATGACATAAAACAAAGCCAGCATGCTGCCCGATATAGCTAG

Protein Analysis

235

Amino Acids

27.01

Weight (kDa)

6.18

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 51 - 204 2e-17 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 111
AccII CGCG 1 cut(s) 56
AciI CCGC 2 cut(s) 5, 432
AclI AACGTT 1 cut(s) 376
AclWI GGATC 2 cut(s) 21, 654
AcoI YGGCCR 3 cut(s) 324, 429, 483
AcsI RAATTY 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 207
AcvI CACGTG 1 cut(s) 407
AfaI GTAC 2 cut(s) 443, 628
AfiI CCNNNNNNNGG 1 cut(s) 502
AgsI TTSAA 2 cut(s) 35, 218
AluBI AGCT 4 cut(s) 191, 342, 643, 702
AluI AGCT 4 cut(s) 191, 342, 643, 702
Alw26I GTCTC 2 cut(s) 426, 441
AlwI GGATC 2 cut(s) 21, 654
AoxI GGCC 3 cut(s) 324, 429, 483
ApeKI GCWGC 2 cut(s) 132, 689
ApoI RAATTY 1 cut(s) 35
AspLEI GCGC 1 cut(s) 177
AsuHPI GGTGA 1 cut(s) 304
BalI TGGCCA 1 cut(s) 485
BanII GRGCYC 1 cut(s) 162
BbrPI CACGTG 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 144, 676
BccI CCATC 1 cut(s) 668
BceAI ACGGC 2 cut(s) 222, 522
BclI TGATCA 1 cut(s) 637
BcoDI GTCTC 2 cut(s) 426, 441
BfaI CTAG 1 cut(s) 703
BfoI RGCGCY 1 cut(s) 178
BisI GCNGC 3 cut(s) 133, 432, 690
BlpI GCTNAGC 2 cut(s) 533, 549
BlsI GCNGC 3 cut(s) 134, 433, 691
BmcAI AGTACT 1 cut(s) 628
BmsI GCATC 2 cut(s) 452, 580
BoxI GACNNNNGTC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 51
Bpu1102I GCTNAGC 2 cut(s) 533, 549
BsaAI YACGTR 1 cut(s) 407
BsaBI GATNNNNATC 2 cut(s) 12, 651
BsaJI CCNNGG 2 cut(s) 480, 486
BsaWI WCCGGW 1 cut(s) 68
Bsc4I CCNNNNNNNGG 1 cut(s) 502
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 133, 253
Bse8I GATNNNNATC 2 cut(s) 12, 651
BseDI CCNNGG 2 cut(s) 480, 486
BseGI GGATG 3 cut(s) 373, 421, 655
BseJI GATNNNNATC 2 cut(s) 12, 651
BseLI CCNNNNNNNGG 1 cut(s) 502
BseMII CTCAG 4 cut(s) 275, 524, 540, 637
BseNI ACTGG 2 cut(s) 133, 253
BseRI GAGGAG 2 cut(s) 288, 291
BseXI GCAGC 2 cut(s) 144, 676
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 3 cut(s) 326, 431, 485
BsiSI CCGG 2 cut(s) 69, 276
BslFI GGGAC 2 cut(s) 483, 557
BslI CCNNNNNNNGG 1 cut(s) 502
BsmAI GTCTC 2 cut(s) 426, 441
BsmFI GGGAC 2 cut(s) 483, 557
BsnI GGCC 3 cut(s) 326, 431, 485
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 4 cut(s) 13, 88, 637, 646
Bsp1720I GCTNAGC 2 cut(s) 533, 549
Bsp19I CCATGG 1 cut(s) 486
Bsp68I TCGCGA 1 cut(s) 56
BspACI CCGC 2 cut(s) 5, 432
BspANI GGCC 3 cut(s) 326, 431, 485
BspCNI CTCAG 4 cut(s) 274, 525, 541, 636
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 1 cut(s) 193
BspPI GGATC 2 cut(s) 21, 654
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 2 cut(s) 133, 253
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 480, 486
BssMI GATC 4 cut(s) 13, 88, 637, 646
BssT1I CCWWGG 2 cut(s) 480, 486
Bst4CI ACNGT 2 cut(s) 50, 64
BstBAI YACGTR 1 cut(s) 407
BstC8I GCNNGC 4 cut(s) 353, 583, 683, 687
BstDEI CTNAG 5 cut(s) 261, 533, 549, 557, 623
BstDSI CCRYGG 1 cut(s) 486
BstENI CCTNNNNNAGG 1 cut(s) 500
BstF5I GGATG 3 cut(s) 373, 421, 655
BstFNI CGCG 1 cut(s) 56
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 4 cut(s) 16, 91, 640, 649
BstMAI GTCTC 2 cut(s) 426, 441
BstMBI GATC 4 cut(s) 13, 88, 637, 646
BstMWI GCNNNNNNNGC 3 cut(s) 132, 348, 462
BstNSI RCATGY 2 cut(s) 401, 689
BstPAI GACNNNNGTC 1 cut(s) 231
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 2 cut(s) 144, 676
BstV2I GAAGAC 1 cut(s) 51
BstXI CCANNNNNNTGG 1 cut(s) 322
BsuRI GGCC 3 cut(s) 326, 431, 485
BtgI CCRYGG 1 cut(s) 486
BtsCI GGATG 3 cut(s) 373, 421, 655
BtsIMutI CAGTG 2 cut(s) 98, 126
BtuMI TCGCGA 1 cut(s) 56
Cac8I GCNNGC 4 cut(s) 353, 583, 683, 687
CciI TCATGA 1 cut(s) 193
CfoI GCGC 1 cut(s) 177
Cfr10I RCCGGY 1 cut(s) 275
CseI GACGC 1 cut(s) 554
Csp6I GTAC 2 cut(s) 442, 627
CviAII CATG 6 cut(s) 122, 137, 194, 398, 487, 686
CviQI GTAC 2 cut(s) 442, 627
DdeI CTNAG 5 cut(s) 261, 533, 549, 557, 623
DpnI GATC 4 cut(s) 15, 90, 639, 648
DpnII GATC 4 cut(s) 13, 88, 637, 646
EaeI YGGCCR 3 cut(s) 324, 429, 483
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 480, 486
Eco24I GRGCYC 1 cut(s) 162
Eco32I GATATC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 207
Eco72I CACGTG 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 35
EcoRV GATATC 1 cut(s) 526
EcoT14I CCWWGG 2 cut(s) 480, 486
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 2 cut(s) 480, 486
FaeI CATG 6 cut(s) 125, 140, 197, 401, 490, 689
FaqI GGGAC 2 cut(s) 483, 557
FatI CATG 6 cut(s) 121, 136, 193, 397, 486, 685
FbaI TGATCA 1 cut(s) 637
Fnu4HI GCNGC 3 cut(s) 133, 432, 690
FokI GGATG 3 cut(s) 380, 428, 642
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 3 cut(s) 133, 432, 690
FspBI CTAG 1 cut(s) 703
GlaI GCGC 1 cut(s) 176
GluI GCNGC 3 cut(s) 133, 432, 690
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 3 cut(s) 326, 431, 485
HapII CCGG 2 cut(s) 69, 276
HgaI GACGC 1 cut(s) 554
HhaI GCGC 1 cut(s) 177
Hin1II CATG 6 cut(s) 125, 140, 197, 401, 490, 689
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HinfI GANTC 3 cut(s) 8, 609, 615
HpaII CCGG 2 cut(s) 69, 276
HphI GGTGA 1 cut(s) 304
Hpy188I TCNGA 2 cut(s) 13, 614
Hpy188III TCNNGA 3 cut(s) 55, 194, 496
Hpy99I CGWCG 2 cut(s) 89, 237
HpyAV CCTTC 1 cut(s) 604
HpyCH4III ACNGT 2 cut(s) 50, 64
HpyCH4IV ACGT 3 cut(s) 228, 376, 406
HpyCH4V TGCA 4 cut(s) 121, 397, 465, 585
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 348, 462
HpyF3I CTNAG 5 cut(s) 261, 533, 549, 557, 623
HpySE526I ACGT 3 cut(s) 228, 376, 406
Hsp92II CATG 6 cut(s) 125, 140, 197, 401, 490, 689
HspAI GCGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 637
Kzo9I GATC 4 cut(s) 13, 88, 637, 646
LmnI GCTCC 1 cut(s) 301
Lsp1109I GCAGC 2 cut(s) 144, 676
LweI GCATC 2 cut(s) 452, 580
MaeI CTAG 1 cut(s) 703
MaeII ACGT 3 cut(s) 228, 376, 406
MaeIII GTNAC 2 cut(s) 64, 224
MalI GATC 4 cut(s) 15, 90, 639, 648
MboI GATC 4 cut(s) 13, 88, 637, 646
MboII GAAGA 2 cut(s) 56, 241
MhlI GDGCHC 1 cut(s) 162
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 3 cut(s) 35, 112, 285
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 1 cut(s) 603
MnlI CCTC 4 cut(s) 254, 309, 312, 358
Mox20I TGGCCA 1 cut(s) 485
MscI TGGCCA 1 cut(s) 485
MseI TTAA 5 cut(s) 99, 288, 360, 372, 474
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 643
MspI CCGG 2 cut(s) 69, 276
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 3 cut(s) 132, 348, 462
NcoI CCATGG 1 cut(s) 486
NdeII GATC 4 cut(s) 13, 88, 637, 646
NlaIII CATG 6 cut(s) 125, 140, 197, 401, 490, 689
NmuCI GTSAC 1 cut(s) 224
NruI TCGCGA 1 cut(s) 56
NspI RCATGY 2 cut(s) 401, 689
PaeI GCATGC 1 cut(s) 689
PagI TCATGA 1 cut(s) 193
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 382
PfeI GAWTC 2 cut(s) 8, 615
PkrI GCNGC 3 cut(s) 134, 433, 691
PleI GAGTC 1 cut(s) 603
PmaCI CACGTG 1 cut(s) 407
PmlI CACGTG 1 cut(s) 407
PpsI GAGTC 1 cut(s) 603
Ppu21I YACGTR 1 cut(s) 407
PshAI GACNNNNGTC 1 cut(s) 231
PsiI TTATAA 1 cut(s) 111
Psp1406I AACGTT 1 cut(s) 376
PspCI CACGTG 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 643
RruI TCGCGA 1 cut(s) 56
RsaI GTAC 2 cut(s) 443, 628
RsaNI GTAC 2 cut(s) 442, 627
SaqAI TTAA 5 cut(s) 99, 288, 360, 372, 474
SatI GCNGC 3 cut(s) 133, 432, 690
Sau3AI GATC 4 cut(s) 13, 88, 637, 646
ScaI AGTACT 1 cut(s) 628
SchI GAGTC 1 cut(s) 603
SduI GDGCHC 1 cut(s) 162
SetI ASST 9 cut(s) 23, 193, 231, 344, 379, 409, 482, 645, 704
SfaNI GCATC 2 cut(s) 452, 580
SphI GCATGC 1 cut(s) 689
Sse9I AATT 3 cut(s) 35, 112, 285
SsiI CCGC 2 cut(s) 5, 432
SspMI CTAG 1 cut(s) 703
StyI CCWWGG 2 cut(s) 480, 486
TaaI ACNGT 2 cut(s) 50, 64
TaiI ACGT 3 cut(s) 231, 379, 409
TaqI TCGA 5 cut(s) 87, 162, 180, 573, 607
TasI AATT 3 cut(s) 35, 112, 285
TatI WGTACW 2 cut(s) 441, 626
TauI GCSGC 1 cut(s) 434
TfiI GAWTC 2 cut(s) 8, 615
Tru1I TTAA 5 cut(s) 99, 288, 360, 372, 474
Tru9I TTAA 5 cut(s) 99, 288, 360, 372, 474
TscAI CASTG 2 cut(s) 98, 133
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 2 cut(s) 132, 689
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 3 cut(s) 57, 158, 524
TspRI CASTG 2 cut(s) 98, 133
XagI CCTNNNNNAGG 1 cut(s) 500
XapI RAATTY 1 cut(s) 35
XceI RCATGY 2 cut(s) 401, 689
XspI CTAG 1 cut(s) 703
ZrmI AGTACT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.