RLG00000036371

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001899
Physical Location & Seq
Forward (+)
79755477 .. 79758549
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036371

Sequence Viewer

Length: 684 bp
ATGCATCCGAGTGATGATCGACAAGTCCTACTAAATTTTCTGCCCAGAATGGCCATCAGCATAGTAGACCACGAATTACCATACGACACCCACAATCTCTACGATGTAACATTTTACGATGATCAAATCCACACCCTAGTCACTCACAGTGCACCCATGGTGGAATCCTGGCTCTCTGAAATCATGCCCATAATCAGTGTAAACCCAATTGTAGGCCTGGACGTGGAGTGGCGTCCTAATTTTAACCGCAACAGCGACCACCCAATTGCGACCTTGCAGCTTTGTGTGGACCATCGTTGCCTCATCTTCCAACTCATCCACGCCCAGAACATCCCACAATCACTCTTTGACTTTCTTGCTAATGGCAGTTACAAATTTGTGGGTGCTGGGATCCAGAATGATACAGAGAAGCTCCTTCTGGACTATGACTTGAAGGTGACCAGTACTATTAATCTTGGGGCTTTGGCAGCGACTAAATTGGGTAAGAGAGACTTGAAGAATGCTGGGTTGAAGGGCCTGTCTAAAGAGGTGCTTGGGAAGGAGCTCCAGAAGTCTAGAAAGGTGACAATGAGTAGGTGGGACAGTGAGTGGCTCACTTGTAATCAGGTTCAGTATGCTTGTATTGATGCTTTTATTTCCTCTGAGATTGGGAGGCAGTTAACTGCCATGGCTGATTGCCAATGA

Protein Analysis

228

Amino Acids

25.69

Weight (kDa)

5.55

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 68 - 222 1.1e-14 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 66
AciI CCGC 1 cut(s) 247
AclWI GGATC 2 cut(s) 385, 398
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 2 cut(s) 34, 374
AcyI GRCGYC 1 cut(s) 232
AfaI GTAC 1 cut(s) 445
AfiI CCNNNNNNNGG 2 cut(s) 212, 223
AgsI TTSAA 3 cut(s) 433, 496, 511
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 2 cut(s) 167, 216
AluBI AGCT 3 cut(s) 280, 412, 544
AluI AGCT 3 cut(s) 280, 412, 544
Alw21I GWGCWC 2 cut(s) 154, 546
Alw26I GTCTC 1 cut(s) 483
Alw44I GTGCAC 1 cut(s) 150
AlwI GGATC 2 cut(s) 385, 398
AoxI GGCC 3 cut(s) 51, 214, 514
ApaLI GTGCAC 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 277, 467
ApoI RAATTY 2 cut(s) 34, 374
AseI ATTAAT 1 cut(s) 450
AspS9I GGNCC 2 cut(s) 289, 514
AsuHPI GGTGA 2 cut(s) 448, 574
AvaII GGWCC 1 cut(s) 289
BaeGI GKGCMC 1 cut(s) 154
BalI TGGCCA 1 cut(s) 53
BamHI GGATCC 1 cut(s) 390
BanII GRGCYC 1 cut(s) 546
Bbv12I GWGCWC 2 cut(s) 154, 546
BbvI GCAGC 2 cut(s) 289, 479
BccI CCATC 2 cut(s) 62, 300
BciT130I CCWGG 2 cut(s) 169, 218
BclI TGATCA 1 cut(s) 121
BcoDI GTCTC 1 cut(s) 483
BfaI CTAG 2 cut(s) 137, 555
BisI GCNGC 2 cut(s) 278, 468
BlsI GCNGC 2 cut(s) 279, 469
BmcAI AGTACT 1 cut(s) 445
Bme1390I CCNGG 2 cut(s) 169, 218
Bme18I GGWCC 1 cut(s) 289
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 289, 514
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 2 cut(s) 169, 218
BmsI GCATC 2 cut(s) 13, 616
BpmI CTGGAG 1 cut(s) 530
BsaHI GRCGYC 1 cut(s) 232
BsaJI CCNNGG 2 cut(s) 156, 666
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 223
Bse1I ACTGG 1 cut(s) 441
BseBI CCWGG 2 cut(s) 169, 218
BseDI CCNNGG 2 cut(s) 156, 666
BseGI GGATG 3 cut(s) 4, 315, 330
BseLI CCNNNNNNNGG 2 cut(s) 212, 223
BseMII CTCAG 1 cut(s) 633
BseNI ACTGG 1 cut(s) 441
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 2 cut(s) 289, 479
BseYI CCCAGC 2 cut(s) 386, 503
BshFI GGCC 3 cut(s) 53, 216, 516
BsiHKAI GWGCWC 2 cut(s) 154, 546
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 2 cut(s) 212, 223
BsmAI GTCTC 1 cut(s) 483
BsmFI GGGAC 1 cut(s) 593
BsmI GAATGC 1 cut(s) 505
BsnI GGCC 3 cut(s) 53, 216, 516
Bsp1286I GDGCHC 2 cut(s) 154, 546
Bsp143I GATC 3 cut(s) 16, 121, 390
Bsp19I CCATGG 2 cut(s) 156, 666
BspACI CCGC 1 cut(s) 247
BspANI GGCC 3 cut(s) 53, 216, 516
BspCNI CTCAG 1 cut(s) 634
BspLI GGNNCC 1 cut(s) 392
BspPI GGATC 2 cut(s) 385, 398
BsrI ACTGG 1 cut(s) 441
BssECI CCNNGG 2 cut(s) 156, 666
BssMI GATC 3 cut(s) 16, 121, 390
BssNI GRCGYC 1 cut(s) 232
BssT1I CCWWGG 2 cut(s) 156, 666
Bst2UI CCWGG 2 cut(s) 169, 218
Bst4CI ACNGT 2 cut(s) 149, 584
BstACI GRCGYC 1 cut(s) 232
BstDEI CTNAG 1 cut(s) 642
BstDSI CCRYGG 2 cut(s) 156, 666
BstEII GGTNACC 1 cut(s) 436
BstF5I GGATG 3 cut(s) 4, 315, 330
BstKTI GATC 3 cut(s) 19, 124, 393
BstMAI GTCTC 1 cut(s) 483
BstMBI GATC 3 cut(s) 16, 121, 390
BstMWI GCNNNNNNNGC 1 cut(s) 467
BstNI CCWGG 2 cut(s) 169, 218
BstPI GGTNACC 1 cut(s) 436
BstSCI CCNGG 2 cut(s) 167, 216
BstSLI GKGCMC 1 cut(s) 154
BstV1I GCAGC 2 cut(s) 289, 479
BstX2I RGATCY 1 cut(s) 390
BstYI RGATCY 1 cut(s) 390
BsuRI GGCC 3 cut(s) 53, 216, 516
BtgI CCRYGG 2 cut(s) 156, 666
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 3 cut(s) 4, 315, 330
BtsIMutI CAGTG 3 cut(s) 154, 202, 589
Cfr13I GGNCC 2 cut(s) 289, 514
CseI GACGC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 444
CviAII CATG 3 cut(s) 157, 184, 667
CviQI GTAC 1 cut(s) 444
DdeI CTNAG 1 cut(s) 642
DpnI GATC 3 cut(s) 18, 123, 392
DpnII GATC 3 cut(s) 16, 121, 390
EaeI YGGCCR 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 544
Eco130I CCWWGG 2 cut(s) 156, 666
Eco147I AGGCCT 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 289
Eco53kI GAGCTC 1 cut(s) 544
Eco91I GGTNACC 1 cut(s) 436
EcoICRI GAGCTC 1 cut(s) 544
EcoO109I RGGNCCY 1 cut(s) 514
EcoO65I GGTNACC 1 cut(s) 436
EcoRII CCWGG 2 cut(s) 167, 216
EcoT14I CCWWGG 2 cut(s) 156, 666
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 2 cut(s) 156, 666
FaeI CATG 3 cut(s) 160, 187, 670
FaiI YATR 8 cut(s) 62, 82, 158, 185, 191, 426, 615, 668
FalI AAGNNNNNCTT 4 cut(s) 476, 508, 516, 548
FaqI GGGAC 1 cut(s) 593
FatI CATG 3 cut(s) 156, 183, 666
FbaI TGATCA 1 cut(s) 121
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 2 cut(s) 278, 468
FokI GGATG 2 cut(s) 302, 317
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 2 cut(s) 278, 468
FspBI CTAG 2 cut(s) 137, 555
GluI GCNGC 2 cut(s) 278, 468
GsaI CCCAGC 2 cut(s) 390, 507
GsuI CTGGAG 1 cut(s) 530
HaeIII GGCC 3 cut(s) 53, 216, 516
HgaI GACGC 1 cut(s) 221
Hin1I GRCGYC 1 cut(s) 232
Hin1II CATG 3 cut(s) 160, 187, 670
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HinfI GANTC 1 cut(s) 164
HpaI GTTAAC 1 cut(s) 660
HphI GGTGA 2 cut(s) 448, 574
Hpy166II GTNNAC 5 cut(s) 67, 152, 202, 289, 660
Hpy188I TCNGA 3 cut(s) 9, 178, 643
Hpy188III TCNNGA 4 cut(s) 394, 419, 547, 555
Hpy8I GTNNAC 5 cut(s) 67, 152, 202, 289, 660
HpyAV CCTTC 4 cut(s) 425, 427, 505, 532
HpyCH4III ACNGT 2 cut(s) 149, 584
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 3 cut(s) 4, 152, 277
HpyF10VI GCNNNNNNNGC 1 cut(s) 467
HpyF3I CTNAG 1 cut(s) 642
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 1 cut(s) 232
Hsp92II CATG 3 cut(s) 160, 187, 670
Ksp22I TGATCA 1 cut(s) 121
KspAI GTTAAC 1 cut(s) 660
Kzo9I GATC 3 cut(s) 16, 121, 390
LmnI GCTCC 3 cut(s) 417, 541, 549
Lsp1109I GCAGC 2 cut(s) 289, 479
LweI GCATC 2 cut(s) 13, 616
MaeI CTAG 2 cut(s) 137, 555
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 5 cut(s) 106, 139, 368, 436, 562
MalI GATC 3 cut(s) 18, 123, 392
MboI GATC 3 cut(s) 16, 121, 390
MboII GAAGA 2 cut(s) 298, 508
MfeI CAATTG 2 cut(s) 207, 264
MflI RGATCY 1 cut(s) 390
MhlI GDGCHC 2 cut(s) 154, 546
MlsI TGGCCA 1 cut(s) 53
MluCI AATT 7 cut(s) 34, 74, 207, 238, 264, 374, 476
MluNI TGGCCA 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 334
MnlI CCTC 4 cut(s) 311, 520, 645, 649
Mox20I TGGCCA 1 cut(s) 53
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 53
MseI TTAA 3 cut(s) 243, 450, 659
MslI CAYNNNNRTG 1 cut(s) 9
Msp20I TGGCCA 1 cut(s) 53
MspR9I CCNGG 2 cut(s) 169, 218
MunI CAATTG 2 cut(s) 207, 264
Mva1269I GAATGC 1 cut(s) 505
MvaI CCWGG 2 cut(s) 169, 218
MwoI GCNNNNNNNGC 1 cut(s) 467
NcoI CCATGG 2 cut(s) 156, 666
NdeII GATC 3 cut(s) 16, 121, 390
NlaIII CATG 3 cut(s) 160, 187, 670
NlaIV GGNNCC 1 cut(s) 392
NmuCI GTSAC 3 cut(s) 139, 436, 562
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 216
PctI GAATGC 1 cut(s) 505
PfeI GAWTC 1 cut(s) 164
PkrI GCNGC 2 cut(s) 279, 469
PshBI ATTAAT 1 cut(s) 450
Psp124BI GAGCTC 1 cut(s) 546
Psp6I CCWGG 2 cut(s) 167, 216
PspEI GGTNACC 1 cut(s) 436
PspFI CCCAGC 2 cut(s) 386, 503
PspGI CCWGG 2 cut(s) 167, 216
PspN4I GGNNCC 1 cut(s) 392
PspPI GGNCC 2 cut(s) 289, 514
PsuI RGATCY 1 cut(s) 390
RsaI GTAC 1 cut(s) 445
RsaNI GTAC 1 cut(s) 444
RseI CAYNNNNRTG 1 cut(s) 9
SacI GAGCTC 1 cut(s) 546
SaqAI TTAA 3 cut(s) 243, 450, 659
SatI GCNGC 2 cut(s) 278, 468
Sau3AI GATC 3 cut(s) 16, 121, 390
Sau96I GGNCC 2 cut(s) 289, 514
ScaI AGTACT 1 cut(s) 445
ScrFI CCNGG 2 cut(s) 169, 218
SduI GDGCHC 2 cut(s) 154, 546
SfaNI GCATC 2 cut(s) 13, 616
SinI GGWCC 1 cut(s) 289
SmiMI CAYNNNNRTG 1 cut(s) 9
Sse9I AATT 7 cut(s) 34, 74, 207, 238, 264, 374, 476
SseBI AGGCCT 1 cut(s) 216
SsiI CCGC 1 cut(s) 247
SspMI CTAG 2 cut(s) 137, 555
SstI GAGCTC 1 cut(s) 546
StuI AGGCCT 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 167, 216
StyI CCWWGG 2 cut(s) 156, 666
TaaI ACNGT 2 cut(s) 149, 584
TaiI ACGT 1 cut(s) 225
TaqI TCGA 1 cut(s) 19
TasI AATT 7 cut(s) 34, 74, 207, 238, 264, 374, 476
TatI WGTACW 1 cut(s) 443
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 3 cut(s) 243, 450, 659
Tru9I TTAA 3 cut(s) 243, 450, 659
TscAI CASTG 3 cut(s) 154, 202, 589
TseFI GTSAC 3 cut(s) 139, 436, 562
TseI GCWGC 2 cut(s) 277, 467
Tsp45I GTSAC 3 cut(s) 139, 436, 562
TspRI CASTG 3 cut(s) 154, 202, 589
VneI GTGCAC 1 cut(s) 150
VpaK11BI GGWCC 1 cut(s) 289
VspI ATTAAT 1 cut(s) 450
XapI RAATTY 2 cut(s) 34, 374
XbaI TCTAGA 1 cut(s) 554
XmiI GTMKAC 1 cut(s) 66
XspI CTAG 2 cut(s) 137, 555
ZrmI AGTACT 1 cut(s) 445
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.