pycom03g20090

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001899
Physical Location & Seq
Forward (+)
20647594 .. 20648400
807 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20090.1

Sequence Viewer

Length: 807 bp
ATGCATGCGACAGCCGACGGGTCTATTTATAACCGTTGGAATTCAAAACGCTCCGTTTGGGGTCGGTTTTCCACTTTATCAAAACCCAACCCTCGCATTGACCAATCCCAGAGGAGGATGGCGATCAGCATCGTGGACTACGAGCTACCGTACGACACCCACAACGTGTACGGCGTGTCGTTTTACAACGACAGAGTCCACACCATGGTCACCAGCAACTCAGCCATGGTGGAATCGTGGCTCTCCAACGTCACTCCCGCCAACCCCAACCACCGCATTGTCGGCCTCGACGTCGAGTGGCGCCCCAACAACCGCTACCACCACAACCCGATCGCCACCCTGCAGCTCTGCACCGGCCCCAACTACCCCGGCTCCCTTCAAATCCCGGCAGGCACGGTGCTGCCCGGCGTCTCCTTGGACCCCAATTGCCTAATATTCCAGGTTTTACACTCCGATTGGATTCCCCATTCCCTATCCACTTTCCTCGCGAATCCCGCCTTCACGTTCGTCGGCGTCGGCATCGAGAGCGATTGCGAGAAGCTGCTTTTAGACTACAGGCTGCGCGTCGCCAATGTTGTTGATCTTCGGGGTTTAGCAGCGGATAGGTTGGGGGTTTGGGAGCTGAGGAACTTCGGGTTGAAGCGGTTGGCGAGCGCGGTGCTGGGGAAGGAGCTGGAGAAGCCGAAGAGCGTGACGTTGAGCAGGTGGGACAGTCAGTGGCTTACTTATGCTCAGATTCAGTATGCTGCTGTTGACGCTTTTATTTCATATGAGATTGCTAGGCAATTGAATCTTAGGGGTGCTTAG

Protein Analysis

269

Amino Acids

30.24

Weight (kDa)

8.75

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 89 - 265 2.5e-15 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 30
AarI CACCTGC 1 cut(s) 693
AatII GACGTC 1 cut(s) 294
Acc36I ACCTGC 1 cut(s) 693
AccB1I GGYRCC 1 cut(s) 300
AccB7I CCANNNNNTGG 1 cut(s) 205
AccII CGCG 3 cut(s) 488, 564, 656
AciI CCGC 7 cut(s) 258, 274, 313, 495, 599, 643, 656
AcsI RAATTY 1 cut(s) 40
AcyI GRCGYC 4 cut(s) 291, 301, 408, 513
AdeI CACNNNGTG 1 cut(s) 166
AfaI GTAC 2 cut(s) 152, 170
AfiI CCNNNNNNNGG 2 cut(s) 114, 205
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 4 cut(s) 45, 380, 640, 790
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 5 cut(s) 145, 346, 541, 622, 673
AluI AGCT 5 cut(s) 145, 346, 541, 622, 673
Alw26I GTCTC 1 cut(s) 415
AoxI GGCC 2 cut(s) 283, 355
ApeKI GCWGC 6 cut(s) 343, 400, 541, 559, 596, 746
ApoI RAATTY 1 cut(s) 40
AspLEI GCGC 3 cut(s) 303, 564, 656
AspS9I GGNCC 2 cut(s) 356, 418
AsuC2I CCSGG 3 cut(s) 369, 386, 405
AsuHPI GGTGA 1 cut(s) 202
AvaII GGWCC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 300
BbvCI CCTCAGC 1 cut(s) 623
BbvI GCAGC 6 cut(s) 355, 387, 528, 546, 608, 733
BccI CCATC 1 cut(s) 112
BceAI ACGGC 1 cut(s) 187
BcgI CGANNNNNNTGC 2 cut(s) 640, 674
BciT130I CCWGG 1 cut(s) 440
BcnI CCSGG 3 cut(s) 369, 386, 405
BcoDI GTCTC 1 cut(s) 415
BfaI CTAG 1 cut(s) 780
BfmI CTRYAG 2 cut(s) 341, 553
BfoI RGCGCY 1 cut(s) 304
BfuAI ACCTGC 1 cut(s) 693
BisI GCNGC 6 cut(s) 344, 401, 542, 560, 597, 747
BlsI GCNGC 6 cut(s) 345, 402, 543, 561, 598, 748
Bme1390I CCNGG 4 cut(s) 369, 386, 405, 440
Bme18I GGWCC 1 cut(s) 418
BmgT120I GGNCC 2 cut(s) 356, 418
BmiI GGNNCC 4 cut(s) 302, 358, 373, 420
BmrFI CCNGG 4 cut(s) 369, 386, 405, 440
BmsI GCATC 2 cut(s) 138, 528
BpmI CTGGAG 1 cut(s) 695
Bpu10I CCTNAGC 1 cut(s) 623
BpuMI CCSGG 3 cut(s) 369, 386, 405
BsaBI GATNNNNATC 2 cut(s) 122, 128
BsaHI GRCGYC 4 cut(s) 291, 301, 408, 513
BsaJI CCNNGG 4 cut(s) 204, 225, 367, 414
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 205
Bse118I RCCGGY 1 cut(s) 353
Bse8I GATNNNNATC 2 cut(s) 122, 128
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 4 cut(s) 204, 225, 367, 414
BseGI GGATG 1 cut(s) 123
BseJI GATNNNNATC 2 cut(s) 122, 128
BseLI CCNNNNNNNGG 2 cut(s) 114, 205
BseMII CTCAG 3 cut(s) 234, 614, 746
BseRI GAGGAG 1 cut(s) 127
BseXI GCAGC 6 cut(s) 355, 387, 528, 546, 608, 733
BseYI CCCAGC 1 cut(s) 661
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 3 cut(s) 488, 564, 656
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 2 cut(s) 285, 357
BshNI GGYRCC 1 cut(s) 300
BsiEI CGRYCG 1 cut(s) 333
BsiSI CCGG 4 cut(s) 354, 369, 386, 405
BsiWI CGTACG 1 cut(s) 150
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 2 cut(s) 114, 205
BsmAI GTCTC 1 cut(s) 415
BsmBI CGTCTC 1 cut(s) 415
BsmFI GGGAC 1 cut(s) 722
BsnI GGCC 2 cut(s) 285, 357
Bsp143I GATC 3 cut(s) 123, 330, 580
Bsp19I CCATGG 2 cut(s) 204, 225
Bsp68I TCGCGA 1 cut(s) 488
BspACI CCGC 7 cut(s) 258, 274, 313, 495, 599, 643, 656
BspANI GGCC 2 cut(s) 285, 357
BspCNI CTCAG 3 cut(s) 233, 615, 745
BspFNI CGCG 3 cut(s) 488, 564, 656
BspLI GGNNCC 4 cut(s) 302, 358, 373, 420
BspMAI CTGCAG 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 693
BspQI GCTCTTC 1 cut(s) 680
BspT107I GGYRCC 1 cut(s) 300
BsrFI RCCGGY 1 cut(s) 353
BssAI RCCGGY 1 cut(s) 353
BssECI CCNNGG 4 cut(s) 204, 225, 367, 414
BssMI GATC 3 cut(s) 123, 330, 580
BssNI GRCGYC 4 cut(s) 291, 301, 408, 513
BssT1I CCWWGG 3 cut(s) 204, 225, 414
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 4 cut(s) 35, 150, 397, 713
Bst6I CTCTTC 1 cut(s) 680
BstACI GRCGYC 4 cut(s) 291, 301, 408, 513
BstC8I GCNNGC 3 cut(s) 6, 391, 652
BstDEI CTNAG 5 cut(s) 220, 623, 732, 794, 804
BstDSI CCRYGG 2 cut(s) 204, 225
BstEII GGTNACC 1 cut(s) 208
BstF5I GGATG 1 cut(s) 123
BstFNI CGCG 3 cut(s) 488, 564, 656
BstH2I RGCGCY 1 cut(s) 304
BstHHI GCGC 3 cut(s) 303, 564, 656
BstKTI GATC 3 cut(s) 126, 333, 583
BstMAI GTCTC 1 cut(s) 415
BstMBI GATC 3 cut(s) 123, 330, 580
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 5 cut(s) 282, 494, 525, 679, 755
BstNI CCWGG 1 cut(s) 440
BstNSI RCATGY 1 cut(s) 8
BstPI GGTNACC 1 cut(s) 208
BstSCI CCNGG 4 cut(s) 367, 384, 403, 438
BstSFI CTRYAG 2 cut(s) 341, 553
BstUI CGCG 3 cut(s) 488, 564, 656
BstV1I GCAGC 6 cut(s) 355, 387, 528, 546, 608, 733
BsuRI GGCC 2 cut(s) 285, 357
BtgI CCRYGG 2 cut(s) 204, 225
BtsCI GGATG 1 cut(s) 123
BtsIMutI CAGTG 1 cut(s) 722
BtuMI TCGCGA 1 cut(s) 488
BveI ACCTGC 1 cut(s) 693
Cac8I GCNNGC 3 cut(s) 6, 391, 652
CfoI GCGC 3 cut(s) 303, 564, 656
Cfr10I RCCGGY 1 cut(s) 353
Cfr13I GGNCC 2 cut(s) 356, 418
CseI GACGC 4 cut(s) 397, 502, 553, 764
Csp6I GTAC 2 cut(s) 151, 169
CviAII CATG 3 cut(s) 5, 205, 226
CviQI GTAC 2 cut(s) 151, 169
DdeI CTNAG 5 cut(s) 220, 623, 732, 794, 804
DinI GGCGCC 1 cut(s) 302
DpnI GATC 3 cut(s) 125, 332, 582
DpnII GATC 3 cut(s) 123, 330, 580
DraIII CACNNNGTG 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 680
EarI CTCTTC 1 cut(s) 680
Eco130I CCWWGG 3 cut(s) 204, 225, 414
Eco47I GGWCC 1 cut(s) 418
Eco91I GGTNACC 1 cut(s) 208
EcoO65I GGTNACC 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 3 cut(s) 204, 225, 414
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 302
EheI GGCGCC 1 cut(s) 302
ErhI CCWWGG 3 cut(s) 204, 225, 414
Esp3I CGTCTC 1 cut(s) 415
FaeI CATG 3 cut(s) 8, 208, 229
FaiI YATR 8 cut(s) 6, 30, 206, 227, 729, 744, 769, 771
FaqI GGGAC 1 cut(s) 722
FatI CATG 3 cut(s) 4, 204, 225
FauI CCCGC 2 cut(s) 265, 502
FauNDI CATATG 1 cut(s) 769
Fnu4HI GCNGC 6 cut(s) 344, 401, 542, 560, 597, 747
FokI GGATG 1 cut(s) 130
Fsp4HI GCNGC 6 cut(s) 344, 401, 542, 560, 597, 747
FspBI CTAG 1 cut(s) 780
GlaI GCGC 3 cut(s) 302, 563, 655
GluI GCNGC 6 cut(s) 344, 401, 542, 560, 597, 747
GsaI CCCAGC 1 cut(s) 665
GsuI CTGGAG 1 cut(s) 695
HaeII RGCGCY 1 cut(s) 304
HaeIII GGCC 2 cut(s) 285, 357
HapII CCGG 4 cut(s) 354, 369, 386, 405
HgaI GACGC 4 cut(s) 397, 502, 553, 764
HhaI GCGC 3 cut(s) 303, 564, 656
Hin1I GRCGYC 4 cut(s) 291, 301, 408, 513
Hin1II CATG 3 cut(s) 8, 208, 229
Hin6I GCGC 3 cut(s) 301, 562, 654
HinP1I GCGC 3 cut(s) 301, 562, 654
HincII GTYRAC 1 cut(s) 754
HindII GTYRAC 1 cut(s) 754
HinfI GANTC 6 cut(s) 195, 233, 460, 490, 736, 790
HpaII CCGG 4 cut(s) 354, 369, 386, 405
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 4 cut(s) 136, 169, 199, 754
Hpy188I TCNGA 2 cut(s) 454, 735
Hpy188III TCNNGA 2 cut(s) 487, 523
Hpy8I GTNNAC 4 cut(s) 136, 169, 199, 754
Hpy99I CGWCG 6 cut(s) 20, 293, 296, 512, 518, 569
HpyAV CCTTC 3 cut(s) 386, 508, 661
HpyCH4III ACNGT 4 cut(s) 35, 150, 397, 713
HpyCH4IV ACGT 5 cut(s) 165, 249, 291, 503, 695
HpyCH4V TGCA 3 cut(s) 4, 343, 351
HpyF10VI GCNNNNNNNGC 5 cut(s) 282, 494, 525, 679, 755
HpyF3I CTNAG 5 cut(s) 220, 623, 732, 794, 804
HpySE526I ACGT 5 cut(s) 165, 249, 291, 503, 695
Hsp92I GRCGYC 4 cut(s) 291, 301, 408, 513
Hsp92II CATG 3 cut(s) 8, 208, 229
HspAI GCGC 3 cut(s) 301, 562, 654
KasI GGCGCC 1 cut(s) 300
Kzo9I GATC 3 cut(s) 123, 330, 580
LguI GCTCTTC 1 cut(s) 680
LmnI GCTCC 4 cut(s) 56, 377, 619, 670
Lsp1109I GCAGC 6 cut(s) 355, 387, 528, 546, 608, 733
LweI GCATC 2 cut(s) 138, 528
MaeI CTAG 1 cut(s) 780
MaeII ACGT 5 cut(s) 165, 249, 291, 503, 695
MaeIII GTNAC 3 cut(s) 208, 250, 691
MalI GATC 3 cut(s) 125, 332, 582
MboI GATC 3 cut(s) 123, 330, 580
MboII GAAGA 2 cut(s) 575, 697
MfeI CAATTG 2 cut(s) 424, 785
MluCI AATT 3 cut(s) 40, 424, 785
Mly113I GGCGCC 1 cut(s) 301
MlyI GAGTC 1 cut(s) 204
MmeI TCCRAC 2 cut(s) 17, 270
MnlI CCTC 6 cut(s) 102, 105, 108, 296, 494, 618
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 1 cut(s) 599
MspI CCGG 4 cut(s) 354, 369, 386, 405
MspR9I CCNGG 4 cut(s) 369, 386, 405, 440
MunI CAATTG 2 cut(s) 424, 785
MvaI CCWGG 1 cut(s) 440
MvnI CGCG 3 cut(s) 488, 564, 656
MwoI GCNNNNNNNGC 5 cut(s) 282, 494, 525, 679, 755
NarI GGCGCC 1 cut(s) 301
NciI CCSGG 3 cut(s) 369, 386, 405
NcoI CCATGG 2 cut(s) 204, 225
NdeI CATATG 1 cut(s) 769
NdeII GATC 3 cut(s) 123, 330, 580
NlaIII CATG 3 cut(s) 8, 208, 229
NlaIV GGNNCC 4 cut(s) 302, 358, 373, 420
NmuCI GTSAC 3 cut(s) 208, 250, 691
NruI TCGCGA 1 cut(s) 488
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PaqCI CACCTGC 1 cut(s) 693
PciSI GCTCTTC 1 cut(s) 680
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 171, 288
PfeI GAWTC 5 cut(s) 233, 460, 490, 736, 790
Pfl23II CGTACG 1 cut(s) 150
PflFI GACNNNGTC 1 cut(s) 194
PflMI CCANNNNNTGG 1 cut(s) 205
PkrI GCNGC 6 cut(s) 345, 402, 543, 561, 598, 748
Ple19I CGATCG 1 cut(s) 333
PleI GAGTC 1 cut(s) 203
PluTI GGCGCC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 203
PsiI TTATAA 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 438
PspEI GGTNACC 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 1 cut(s) 438
PspLI CGTACG 1 cut(s) 150
PspN4I GGNNCC 4 cut(s) 302, 358, 373, 420
PspPI GGNCC 2 cut(s) 356, 418
PstI CTGCAG 1 cut(s) 345
PsyI GACNNNGTC 1 cut(s) 194
PvuI CGATCG 1 cut(s) 333
RruI TCGCGA 1 cut(s) 488
RsaI GTAC 2 cut(s) 152, 170
RsaNI GTAC 2 cut(s) 151, 169
SapI GCTCTTC 1 cut(s) 680
SatI GCNGC 6 cut(s) 344, 401, 542, 560, 597, 747
Sau3AI GATC 3 cut(s) 123, 330, 580
Sau96I GGNCC 2 cut(s) 356, 418
SchI GAGTC 1 cut(s) 204
ScrFI CCNGG 4 cut(s) 369, 386, 405, 440
SfaNI GCATC 2 cut(s) 138, 528
SfcI CTRYAG 2 cut(s) 341, 553
SfoI GGCGCC 1 cut(s) 302
SinI GGWCC 1 cut(s) 418
SphI GCATGC 1 cut(s) 8
Sse9I AATT 3 cut(s) 40, 424, 785
SsiI CCGC 7 cut(s) 258, 274, 313, 495, 599, 643, 656
SspDI GGCGCC 1 cut(s) 300
SspI AATATT 1 cut(s) 435
SspMI CTAG 1 cut(s) 780
StyD4I CCNGG 4 cut(s) 367, 384, 403, 438
StyI CCWWGG 3 cut(s) 204, 225, 414
TaaI ACNGT 4 cut(s) 35, 150, 397, 713
TaiI ACGT 5 cut(s) 168, 252, 294, 506, 698
TaqI TCGA 3 cut(s) 288, 294, 522
TasI AATT 3 cut(s) 40, 424, 785
TfiI GAWTC 5 cut(s) 233, 460, 490, 736, 790
TscAI CASTG 1 cut(s) 722
TseFI GTSAC 3 cut(s) 208, 250, 691
TseI GCWGC 6 cut(s) 343, 400, 541, 559, 596, 746
Tsp45I GTSAC 3 cut(s) 208, 250, 691
TspDTI ATGAA 1 cut(s) 756
TspGWI ACGGA 1 cut(s) 43
TspRI CASTG 1 cut(s) 722
Tth111I GACNNNGTC 1 cut(s) 194
Van91I CCANNNNNTGG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 418
XapI RAATTY 1 cut(s) 40
XceI RCATGY 1 cut(s) 8
XspI CTAG 1 cut(s) 780
ZraI GACGTC 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.