MD03G1253300.v1.1

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001899
Physical Location & Seq
Forward (+)
34034617 .. 34035306
690 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1253300.v1.1.491

Sequence Viewer

Length: 612 bp
ATGGCGATCAGCATCGTGGACTACGAGCTACCGTACGACACCCACAACGTGTACGACGTGTCGTTTTACAACGACAGAGTCCACACCATGGTCACCAGCAACTCAGCCATGGTGGAATCGTGGCTCTCCAACGTCACTCCCGCCAACCCCAACCACCGCATTGTTGGCCTCGACGTCGAGTGGCGCCCCAACAACCGCTACTACCACAACCCGATCGCCACCCTGCAGCTCTGCACCGGCCCCAACTACCCCGGCTCCCTTCAAATCCCGGCAGGCATGGTGCTGCCCGGCGTCTGCTTGGACCCCAATTGCCTAATATTCCAGATTTTACACTCCGAATTGATTCCCCATTCCCTATCCACTTTCCTCGCCAATCCTGCCTTCACGTTCGTCGGCGTCGGCATCGAGAGCGATTGCGAGAAGCTGCTTTCAGACTACAGGCTGCGCGTCGCCAACGTTGTTGATCTTCGGGGTTTAGCAGCGGATAGGTTGGGGGTTTGGGAGCTGAGGAACTTCGGGTTGAAGCGGTTGGCAAGCGCGGTGCTGGGGAAGGAGCTGGAGAAGCCGAAGAGTGTGACGTTGAGCAGGCAATTGAATCTTAGGGGTGCTTAG

Protein Analysis

204

Amino Acids

22.62

Weight (kDa)

6.29

Isoelectric Point (pI)

31.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 50 - 192 1.1e-07 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 183
AccB7I CCANNNNNTGG 1 cut(s) 88
AccII CGCG 2 cut(s) 447, 539
AciI CCGC 6 cut(s) 141, 157, 196, 482, 526, 539
AclI AACGTT 1 cut(s) 456
AcyI GRCGYC 4 cut(s) 174, 184, 291, 396
AdeI CACNNNGTG 1 cut(s) 49
AfaI GTAC 2 cut(s) 35, 53
AfiI CCNNNNNNNGG 1 cut(s) 88
AflIII ACRYGT 2 cut(s) 48, 57
AgsI TTSAA 3 cut(s) 263, 523, 595
AjiI CACGTC 1 cut(s) 58
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 5 cut(s) 28, 229, 424, 505, 556
AluI AGCT 5 cut(s) 28, 229, 424, 505, 556
AoxI GGCC 2 cut(s) 166, 238
ApeKI GCWGC 5 cut(s) 226, 283, 424, 442, 479
Asp700I GAANNNNTTC 1 cut(s) 342
AspLEI GCGC 3 cut(s) 186, 447, 539
AspS9I GGNCC 2 cut(s) 239, 301
AsuC2I CCSGG 3 cut(s) 252, 269, 288
AsuHPI GGTGA 1 cut(s) 85
AvaII GGWCC 1 cut(s) 301
BanI GGYRCC 1 cut(s) 183
BbvCI CCTCAGC 1 cut(s) 506
BbvI GCAGC 5 cut(s) 238, 270, 411, 429, 491
BcnI CCSGG 3 cut(s) 252, 269, 288
BfmI CTRYAG 2 cut(s) 224, 436
BfoI RGCGCY 1 cut(s) 187
BisI GCNGC 5 cut(s) 227, 284, 425, 443, 480
BlsI GCNGC 5 cut(s) 228, 285, 426, 444, 481
Bme1390I CCNGG 3 cut(s) 252, 269, 288
Bme18I GGWCC 1 cut(s) 301
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 239, 301
BmiI GGNNCC 4 cut(s) 185, 241, 256, 303
BmrFI CCNGG 3 cut(s) 252, 269, 288
BmsI GCATC 2 cut(s) 21, 411
BpmI CTGGAG 1 cut(s) 578
Bpu10I CCTNAGC 1 cut(s) 506
BpuMI CCSGG 3 cut(s) 252, 269, 288
BsaBI GATNNNNATC 1 cut(s) 11
BsaHI GRCGYC 4 cut(s) 174, 184, 291, 396
BsaJI CCNNGG 3 cut(s) 87, 108, 250
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 1 cut(s) 88
Bse118I RCCGGY 1 cut(s) 236
Bse8I GATNNNNATC 1 cut(s) 11
BseDI CCNNGG 3 cut(s) 87, 108, 250
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 1 cut(s) 88
BseMII CTCAG 2 cut(s) 117, 497
BseXI GCAGC 5 cut(s) 238, 270, 411, 429, 491
BseYI CCCAGC 1 cut(s) 544
BsgI GTGCAG 1 cut(s) 217
Bsh1236I CGCG 2 cut(s) 447, 539
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 2 cut(s) 168, 240
BshNI GGYRCC 1 cut(s) 183
BsiEI CGRYCG 1 cut(s) 216
BsiSI CCGG 4 cut(s) 237, 252, 269, 288
BsiWI CGTACG 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 88
BsnI GGCC 2 cut(s) 168, 240
Bsp143I GATC 3 cut(s) 6, 213, 463
Bsp19I CCATGG 2 cut(s) 87, 108
BspACI CCGC 6 cut(s) 141, 157, 196, 482, 526, 539
BspANI GGCC 2 cut(s) 168, 240
BspCNI CTCAG 2 cut(s) 116, 498
BspFNI CGCG 2 cut(s) 447, 539
BspLI GGNNCC 4 cut(s) 185, 241, 256, 303
BspMAI CTGCAG 1 cut(s) 228
BspT107I GGYRCC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 3 cut(s) 87, 108, 250
BssMI GATC 3 cut(s) 6, 213, 463
BssNI GRCGYC 4 cut(s) 174, 184, 291, 396
BssT1I CCWWGG 2 cut(s) 87, 108
Bst4CI ACNGT 1 cut(s) 33
Bst6I CTCTTC 1 cut(s) 563
BstACI GRCGYC 4 cut(s) 174, 184, 291, 396
BstC8I GCNNGC 3 cut(s) 274, 535, 587
BstDEI CTNAG 4 cut(s) 103, 506, 599, 609
BstDSI CCRYGG 2 cut(s) 87, 108
BstEII GGTNACC 1 cut(s) 91
BstFNI CGCG 2 cut(s) 447, 539
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 3 cut(s) 186, 447, 539
BstKTI GATC 3 cut(s) 9, 216, 466
BstMBI GATC 3 cut(s) 6, 213, 463
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 4 cut(s) 165, 377, 408, 562
BstPI GGTNACC 1 cut(s) 91
BstSCI CCNGG 3 cut(s) 250, 267, 286
BstSFI CTRYAG 2 cut(s) 224, 436
BstUI CGCG 2 cut(s) 447, 539
BstV1I GCAGC 5 cut(s) 238, 270, 411, 429, 491
BsuRI GGCC 2 cut(s) 168, 240
BtgI CCRYGG 2 cut(s) 87, 108
BtrI CACGTC 1 cut(s) 58
Cac8I GCNNGC 3 cut(s) 274, 535, 587
CfoI GCGC 3 cut(s) 186, 447, 539
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 2 cut(s) 239, 301
CseI GACGC 3 cut(s) 280, 385, 436
Csp6I GTAC 2 cut(s) 34, 52
CviAII CATG 3 cut(s) 88, 109, 277
CviQI GTAC 2 cut(s) 34, 52
DdeI CTNAG 4 cut(s) 103, 506, 599, 609
DinI GGCGCC 1 cut(s) 185
DpnI GATC 3 cut(s) 8, 215, 465
DpnII GATC 3 cut(s) 6, 213, 463
DraIII CACNNNGTG 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco130I CCWWGG 2 cut(s) 87, 108
Eco47I GGWCC 1 cut(s) 301
Eco91I GGTNACC 1 cut(s) 91
EcoO65I GGTNACC 1 cut(s) 91
EcoT14I CCWWGG 2 cut(s) 87, 108
EgeI GGCGCC 1 cut(s) 185
EheI GGCGCC 1 cut(s) 185
ErhI CCWWGG 2 cut(s) 87, 108
FaeI CATG 3 cut(s) 91, 112, 280
FaiI YATR 3 cut(s) 89, 110, 278
FatI CATG 3 cut(s) 87, 108, 276
FauI CCCGC 1 cut(s) 148
Fnu4HI GCNGC 5 cut(s) 227, 284, 425, 443, 480
Fsp4HI GCNGC 5 cut(s) 227, 284, 425, 443, 480
GlaI GCGC 3 cut(s) 185, 446, 538
GluI GCNGC 5 cut(s) 227, 284, 425, 443, 480
GsaI CCCAGC 1 cut(s) 548
GsuI CTGGAG 1 cut(s) 578
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 2 cut(s) 168, 240
HapII CCGG 4 cut(s) 237, 252, 269, 288
HgaI GACGC 3 cut(s) 280, 385, 436
HhaI GCGC 3 cut(s) 186, 447, 539
Hin1I GRCGYC 4 cut(s) 174, 184, 291, 396
Hin1II CATG 3 cut(s) 91, 112, 280
Hin6I GCGC 3 cut(s) 184, 445, 537
HinP1I GCGC 3 cut(s) 184, 445, 537
HinfI GANTC 4 cut(s) 78, 116, 343, 595
HpaII CCGG 4 cut(s) 237, 252, 269, 288
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 3 cut(s) 19, 52, 82
Hpy188I TCNGA 2 cut(s) 337, 433
Hpy188III TCNNGA 2 cut(s) 322, 406
Hpy8I GTNNAC 3 cut(s) 19, 52, 82
Hpy99I CGWCG 6 cut(s) 59, 176, 179, 395, 401, 452
HpyAV CCTTC 3 cut(s) 269, 391, 544
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4IV ACGT 7 cut(s) 48, 57, 132, 174, 386, 456, 578
HpyCH4V TGCA 2 cut(s) 226, 234
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 377, 408, 562
HpyF3I CTNAG 4 cut(s) 103, 506, 599, 609
HpySE526I ACGT 7 cut(s) 48, 57, 132, 174, 386, 456, 578
Hsp92I GRCGYC 4 cut(s) 174, 184, 291, 396
Hsp92II CATG 3 cut(s) 91, 112, 280
HspAI GCGC 3 cut(s) 184, 445, 537
KasI GGCGCC 1 cut(s) 183
Kzo9I GATC 3 cut(s) 6, 213, 463
LmnI GCTCC 3 cut(s) 260, 502, 553
Lsp1109I GCAGC 5 cut(s) 238, 270, 411, 429, 491
LweI GCATC 2 cut(s) 21, 411
MaeII ACGT 7 cut(s) 48, 57, 132, 174, 386, 456, 578
MaeIII GTNAC 3 cut(s) 91, 133, 574
MalI GATC 3 cut(s) 8, 215, 465
MboI GATC 3 cut(s) 6, 213, 463
MboII GAAGA 2 cut(s) 458, 580
MfeI CAATTG 2 cut(s) 307, 590
MluCI AATT 3 cut(s) 307, 338, 590
Mly113I GGCGCC 1 cut(s) 184
MlyI GAGTC 1 cut(s) 87
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 3 cut(s) 179, 377, 501
MroXI GAANNNNTTC 1 cut(s) 342
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 4 cut(s) 237, 252, 269, 288
MspR9I CCNGG 3 cut(s) 252, 269, 288
MunI CAATTG 2 cut(s) 307, 590
MvnI CGCG 2 cut(s) 447, 539
MwoI GCNNNNNNNGC 4 cut(s) 165, 377, 408, 562
NarI GGCGCC 1 cut(s) 184
NciI CCSGG 3 cut(s) 252, 269, 288
NcoI CCATGG 2 cut(s) 87, 108
NdeII GATC 3 cut(s) 6, 213, 463
NlaIII CATG 3 cut(s) 91, 112, 280
NlaIV GGNNCC 4 cut(s) 185, 241, 256, 303
NmuCI GTSAC 3 cut(s) 91, 133, 574
PcsI WCGNNNNNNNCGW 2 cut(s) 21, 54
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 3 cut(s) 116, 343, 595
Pfl23II CGTACG 1 cut(s) 33
PflFI GACNNNGTC 1 cut(s) 77
PflMI CCANNNNNTGG 1 cut(s) 88
PkrI GCNGC 5 cut(s) 228, 285, 426, 444, 481
Ple19I CGATCG 1 cut(s) 216
PleI GAGTC 1 cut(s) 86
PluTI GGCGCC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 86
Psp1406I AACGTT 1 cut(s) 456
PspEI GGTNACC 1 cut(s) 91
PspFI CCCAGC 1 cut(s) 544
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 4 cut(s) 185, 241, 256, 303
PspPI GGNCC 2 cut(s) 239, 301
PstI CTGCAG 1 cut(s) 228
PsyI GACNNNGTC 1 cut(s) 77
PvuI CGATCG 1 cut(s) 216
RsaI GTAC 2 cut(s) 35, 53
RsaNI GTAC 2 cut(s) 34, 52
SatI GCNGC 5 cut(s) 227, 284, 425, 443, 480
Sau3AI GATC 3 cut(s) 6, 213, 463
Sau96I GGNCC 2 cut(s) 239, 301
SchI GAGTC 1 cut(s) 87
ScrFI CCNGG 3 cut(s) 252, 269, 288
SfaNI GCATC 2 cut(s) 21, 411
SfcI CTRYAG 2 cut(s) 224, 436
SfoI GGCGCC 1 cut(s) 185
SinI GGWCC 1 cut(s) 301
Sse9I AATT 3 cut(s) 307, 338, 590
SsiI CCGC 6 cut(s) 141, 157, 196, 482, 526, 539
SspDI GGCGCC 1 cut(s) 183
SspI AATATT 1 cut(s) 318
StyD4I CCNGG 3 cut(s) 250, 267, 286
StyI CCWWGG 2 cut(s) 87, 108
TaaI ACNGT 1 cut(s) 33
TaiI ACGT 7 cut(s) 51, 60, 135, 177, 389, 459, 581
TaqI TCGA 3 cut(s) 171, 177, 405
TasI AATT 3 cut(s) 307, 338, 590
TfiI GAWTC 3 cut(s) 116, 343, 595
TseFI GTSAC 3 cut(s) 91, 133, 574
TseI GCWGC 5 cut(s) 226, 283, 424, 442, 479
Tsp45I GTSAC 3 cut(s) 91, 133, 574
Tth111I GACNNNGTC 1 cut(s) 77
Van91I CCANNNNNTGG 1 cut(s) 88
VpaK11BI GGWCC 1 cut(s) 301
XcmI CCANNNNNNNNNTGG 1 cut(s) 161
XmnI GAANNNNTTC 1 cut(s) 342
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.