MD14G1051900.v1.1

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001899
Physical Location & Seq
Reverse (-)
4981404 .. 4982039
636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1051900.v1.1.491

Sequence Viewer

Length: 636 bp
ATGTCTATCAGCATCACAGACTACGAGTTGCGCTACGACACGCACAGCATCTACAGAGTAGATTTCTTCGATGACTACATCGAAACCGTAGTCACGCACACTCCATCCAAGGTCAAGTACTGGATCAACAAAACCCGATACCTACACCGTTACCGCCTTCACAAACTCATCGTTGGCCTCGATGTTGAGTGGCGTCCCAGTTTCACTCGCGGTGTGAACAACCCGGTCGCAACCCTACAATTGTGTGTTGGCCACCGCTGCCTCATATTTCAGTTGCTTCATACCCCGGGAATCCCCAGGTGTCTTTTCAACTTTCTGGCGGATCCGGACTTTACCTTCGTCGGCGTTGGGATCAAGGACGATGTTCGAAAGCTTTGCCTTGAGTATGACCTTGCGGTAGCAAAGTATGTCGATCTTAGGGAATTGGAGGCGAGAAGGTATGGCTGGAGTGTGCAGCGGAGAATAGGGCTAAAGGACATGGCAGCAGATGTTCTAGGGCAGGAATTTCAAAAGCCAAAGACTGTTACAATGAGTCACTGGGATAAGCCGTCCCTTTCACTTGAGCAAATCAAGTATGCTTGCATCGATGCTTTTGTTTCTTTTGAAATTGGGAGGGCTTTGGGCGCAGCGAATTAA

Protein Analysis

212

Amino Acids

24.51

Weight (kDa)

8.54

Isoelectric Point (pI)

25.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 49 - 207 5.9e-16 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 210
AccIII TCCGGA 1 cut(s) 325
AciI CCGC 6 cut(s) 154, 210, 256, 320, 395, 457
AclWI GGATC 4 cut(s) 131, 317, 330, 359
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 1 cut(s) 119
AgsI TTSAA 3 cut(s) 310, 509, 605
AjnI CCWGG 1 cut(s) 296
AluBI AGCT 1 cut(s) 373
AluI AGCT 1 cut(s) 373
AlwI GGATC 4 cut(s) 131, 317, 330, 359
Ama87I CYCGRG 1 cut(s) 286
Aor13HI TCCGGA 1 cut(s) 325
AoxI GGCC 2 cut(s) 175, 250
ApeKI GCWGC 4 cut(s) 258, 454, 482, 626
ApoI RAATTY 1 cut(s) 503
ArsI GACNNNNNNTTYG 4 cut(s) 75, 107, 320, 352
AspLEI GCGC 2 cut(s) 33, 626
AsuC2I CCSGG 3 cut(s) 224, 287, 288
AsuII TTCGAA 1 cut(s) 367
AvaI CYCGRG 1 cut(s) 286
BalI TGGCCA 1 cut(s) 252
BamHI GGATCC 1 cut(s) 322
BbvI GCAGC 3 cut(s) 245, 466, 494
BccI CCATC 1 cut(s) 112
BceAI ACGGC 1 cut(s) 532
BcgI CGANNNNNNTGC 2 cut(s) 357, 391
BciT130I CCWGG 1 cut(s) 298
BcnI CCSGG 3 cut(s) 224, 287, 288
BfaI CTAG 1 cut(s) 494
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 4 cut(s) 259, 455, 483, 627
BlsI GCNGC 4 cut(s) 260, 456, 484, 628
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 4 cut(s) 224, 287, 288, 298
BmeT110I CYCGRG 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 4 cut(s) 224, 287, 288, 298
BmrI ACTGGG 2 cut(s) 192, 547
BmsI GCATC 4 cut(s) 21, 57, 577, 591
BmuI ACTGGG 2 cut(s) 192, 547
BpmI CTGGAG 1 cut(s) 466
Bpu14I TTCGAA 1 cut(s) 367
BpuEI CTTGAG 2 cut(s) 401, 581
BpuMI CCSGG 3 cut(s) 224, 287, 288
Bsa29I ATCGAT 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 193
BsaJI CCNNGG 4 cut(s) 108, 285, 286, 296
BsaWI WCCGGW 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 85, 115
Bse1I ACTGG 3 cut(s) 125, 198, 542
BseAI TCCGGA 1 cut(s) 325
BseBI CCWGG 1 cut(s) 298
BseCI ATCGAT 1 cut(s) 585
BseDI CCNNGG 4 cut(s) 108, 285, 286, 296
BseGI GGATG 1 cut(s) 104
BseNI ACTGG 3 cut(s) 125, 198, 542
BseXI GCAGC 3 cut(s) 245, 466, 494
BsgI GTGCAG 1 cut(s) 473
Bsh1236I CGCG 1 cut(s) 210
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 2 cut(s) 177, 252
BshVI ATCGAT 1 cut(s) 585
BsiEI CGRYCG 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 286
BsiSI CCGG 3 cut(s) 224, 287, 326
BslFI GGGAC 2 cut(s) 180, 535
BsmFI GGGAC 2 cut(s) 180, 535
BsnI GGCC 2 cut(s) 177, 252
BsoBI CYCGRG 1 cut(s) 286
Bsp119I TTCGAA 1 cut(s) 367
Bsp13I TCCGGA 1 cut(s) 325
Bsp143I GATC 4 cut(s) 123, 322, 351, 412
BspACI CCGC 6 cut(s) 154, 210, 256, 320, 395, 457
BspANI GGCC 2 cut(s) 177, 252
BspDI ATCGAT 1 cut(s) 585
BspEI TCCGGA 1 cut(s) 325
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 4 cut(s) 131, 317, 330, 359
BspT104I TTCGAA 1 cut(s) 367
BsrI ACTGG 3 cut(s) 125, 198, 542
BssECI CCNNGG 4 cut(s) 108, 285, 286, 296
BssMI GATC 4 cut(s) 123, 322, 351, 412
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 3 cut(s) 88, 149, 523
BstACI GRCGYC 1 cut(s) 193
BstBI TTCGAA 1 cut(s) 367
BstC8I GCNNGC 1 cut(s) 580
BstDEI CTNAG 1 cut(s) 416
BstF5I GGATG 1 cut(s) 104
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 2 cut(s) 33, 626
BstKTI GATC 4 cut(s) 126, 325, 354, 415
BstMBI GATC 4 cut(s) 123, 322, 351, 412
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 2 cut(s) 258, 623
BstNI CCWGG 1 cut(s) 298
BstSCI CCNGG 4 cut(s) 222, 285, 286, 296
BstSFI CTRYAG 1 cut(s) 52
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 3 cut(s) 245, 466, 494
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
Bsu15I ATCGAT 1 cut(s) 585
BsuRI GGCC 2 cut(s) 177, 252
BsuTUI ATCGAT 1 cut(s) 585
BtsCI GGATG 1 cut(s) 104
BtsIMutI CAGTG 1 cut(s) 535
Cac8I GCNNGC 1 cut(s) 580
CfoI GCGC 2 cut(s) 33, 626
Cfr9I CCCGGG 1 cut(s) 286
ClaI ATCGAT 1 cut(s) 585
CseI GACGC 1 cut(s) 182
Csp6I GTAC 1 cut(s) 118
CviAII CATG 1 cut(s) 478
CviJI RGCY 8 cut(s) 177, 252, 373, 444, 469, 514, 547, 617
CviKI_1 RGCY 8 cut(s) 177, 252, 373, 444, 469, 514, 547, 617
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 1 cut(s) 416
DpnI GATC 4 cut(s) 125, 324, 353, 414
DpnII GATC 4 cut(s) 123, 322, 351, 412
EaeI YGGCCR 1 cut(s) 250
EciI GGCGGA 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 1 cut(s) 481
FaiI YATR 7 cut(s) 266, 282, 387, 408, 441, 479, 576
FaqI GGGAC 2 cut(s) 180, 535
FatI CATG 1 cut(s) 477
Fnu4HI GCNGC 4 cut(s) 259, 455, 483, 627
FokI GGATG 1 cut(s) 91
Fsp4HI GCNGC 4 cut(s) 259, 455, 483, 627
FspBI CTAG 1 cut(s) 494
GlaI GCGC 2 cut(s) 32, 625
GluI GCNGC 4 cut(s) 259, 455, 483, 627
GsuI CTGGAG 1 cut(s) 466
HaeIII GGCC 2 cut(s) 177, 252
HapII CCGG 3 cut(s) 224, 287, 326
HgaI GACGC 1 cut(s) 182
HhaI GCGC 2 cut(s) 33, 626
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 1 cut(s) 481
Hin6I GCGC 2 cut(s) 31, 624
HinP1I GCGC 2 cut(s) 31, 624
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 2 cut(s) 291, 532
HpaII CCGG 3 cut(s) 224, 287, 326
Hpy166II GTNNAC 1 cut(s) 217
Hpy188III TCNNGA 1 cut(s) 326
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 3 cut(s) 167, 346, 429
HpyCH4III ACNGT 3 cut(s) 88, 149, 523
HpyCH4V TGCA 2 cut(s) 454, 582
HpyF10VI GCNNNNNNNGC 2 cut(s) 258, 623
HpyF3I CTNAG 1 cut(s) 416
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 1 cut(s) 481
HspAI GCGC 2 cut(s) 31, 624
Kpn2I TCCGGA 1 cut(s) 325
Kzo9I GATC 4 cut(s) 123, 322, 351, 412
Lsp1109I GCAGC 3 cut(s) 245, 466, 494
LweI GCATC 4 cut(s) 21, 57, 577, 591
MaeI CTAG 1 cut(s) 494
MaeIII GTNAC 4 cut(s) 91, 149, 523, 533
MalI GATC 4 cut(s) 125, 324, 353, 414
MboI GATC 4 cut(s) 123, 322, 351, 412
MboII GAAGA 1 cut(s) 58
MfeI CAATTG 1 cut(s) 239
MflI RGATCY 1 cut(s) 322
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 5 cut(s) 239, 422, 503, 606, 631
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 541
MnlI CCTC 4 cut(s) 188, 272, 421, 606
Mox20I TGGCCA 1 cut(s) 252
MroI TCCGGA 1 cut(s) 325
MscI TGGCCA 1 cut(s) 252
MseI TTAA 1 cut(s) 634
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 2 cut(s) 258, 457
MspI CCGG 3 cut(s) 224, 287, 326
MspR9I CCNGG 4 cut(s) 224, 287, 288, 298
MunI CAATTG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 298
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 2 cut(s) 258, 623
NciI CCSGG 3 cut(s) 224, 287, 288
NdeII GATC 4 cut(s) 123, 322, 351, 412
NlaIII CATG 1 cut(s) 481
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 2 cut(s) 91, 533
NspV TTCGAA 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PfeI GAWTC 1 cut(s) 291
PkrI GCNGC 4 cut(s) 260, 456, 484, 628
PleI GAGTC 1 cut(s) 540
PpsI GAGTC 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 296
PspGI CCWGG 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 324
PsuI RGATCY 1 cut(s) 322
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
SaqAI TTAA 1 cut(s) 634
SatI GCNGC 4 cut(s) 259, 455, 483, 627
Sau3AI GATC 4 cut(s) 123, 322, 351, 412
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 1 cut(s) 541
ScrFI CCNGG 4 cut(s) 224, 287, 288, 298
SetI ASST 7 cut(s) 114, 144, 302, 338, 375, 393, 440
SfaNI GCATC 4 cut(s) 21, 57, 577, 591
SfcI CTRYAG 1 cut(s) 52
SfuI TTCGAA 1 cut(s) 367
SmaI CCCGGG 1 cut(s) 288
SmlI CTYRAG 2 cut(s) 380, 560
SmoI CTYRAG 2 cut(s) 380, 560
Sse9I AATT 5 cut(s) 239, 422, 503, 606, 631
SsiI CCGC 6 cut(s) 154, 210, 256, 320, 395, 457
SspMI CTAG 1 cut(s) 494
StyD4I CCNGG 4 cut(s) 222, 285, 286, 296
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 3 cut(s) 88, 149, 523
TaqI TCGA 6 cut(s) 69, 81, 180, 367, 411, 585
TasI AATT 5 cut(s) 239, 422, 503, 606, 631
TatI WGTACW 1 cut(s) 117
TfiI GAWTC 1 cut(s) 291
Tru1I TTAA 1 cut(s) 634
Tru9I TTAA 1 cut(s) 634
TscAI CASTG 1 cut(s) 542
TseFI GTSAC 2 cut(s) 91, 533
TseI GCWGC 4 cut(s) 258, 454, 482, 626
Tsp45I GTSAC 2 cut(s) 91, 533
TspDTI ATGAA 1 cut(s) 269
TspMI CCCGGG 1 cut(s) 286
TspRI CASTG 1 cut(s) 542
XapI RAATTY 1 cut(s) 503
XmaI CCCGGG 1 cut(s) 286
XspI CTAG 1 cut(s) 494
ZrmI AGTACT 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.