MD09G1279200.v1.1

Werner Syndrome-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001899
Physical Location & Seq
Forward (+)
35627632 .. 35628566
935 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1279200.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGCGATCAGCATAGTGGACTACGAGCTACCGTACGACACCCACAACGAGTACGACGTGACGTTTTACGGCGACAGAATCCACACCATGCTCACCAACAGCCCCGCCATGGTGGAATCCTGGCTCTCCACCGTCACCCTCGCCAACCCCAACCACCGCATCGTCGGCCTTGACGTCGAGTGGCGCCCTAACTTCAGCCGCAACTCTGACAACCCGATCGCCACCCTGCAGCTCTGCACAGGCCCCAACTACGCTGGCTCCCTCCAAATCCCGCCCGGCACTGTGCTTCCCGGCGTCTGCTTGGACCCCAATTGCCTCATCTTCCAGGTCATACACTCAGAATCGATTCCCCAGTCCCTATCCAACTTCCTCGCCAATCCCGCCTTCACGTTCGTCGGCGTCGGGATCGAGAGCGATTGTGAGAAGCTACTTTTGGACTACGAGCTGCGGGTCGCCAATGCGGTCGATCTTCGGGGTTTCGCGGCGCGTAGGTTGGGGTTTCCGGACCTGAGGAACGCCGGGTTGAAGCGGTTGGCGAGCGCAGTGCTGGGGAAGGAGCTGCAGAAGCCGAAGAGGGTGACGATGAGCAGATGGTTCTGA

Protein Analysis

200

Amino Acids

22.14

Weight (kDa)

5.78

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_A_exo1 PF01612 51 - 192 1.6e-08 3'-5' exonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 177
AccB1I GGYRCC 1 cut(s) 183
AccII CGCG 2 cut(s) 482, 487
AccIII TCCGGA 1 cut(s) 502
AciI CCGC 9 cut(s) 105, 157, 199, 272, 381, 448, 461, 482, 529
AclWI GGATC 1 cut(s) 413
AcuI CTGAAG 1 cut(s) 178
AcyI GRCGYC 4 cut(s) 174, 184, 294, 399
AfaI GTAC 2 cut(s) 35, 53
AfiI CCNNNNNNNGG 1 cut(s) 109
AgsI TTSAA 1 cut(s) 526
AjiI CACGTC 1 cut(s) 58
AjnI CCWGG 2 cut(s) 119, 324
AjuI GAANNNNNNNTTGG 4 cut(s) 368, 400, 416, 448
AluBI AGCT 5 cut(s) 28, 232, 427, 445, 559
AluI AGCT 5 cut(s) 28, 232, 427, 445, 559
AlwI GGATC 1 cut(s) 413
Aor13HI TCCGGA 1 cut(s) 502
AoxI GGCC 2 cut(s) 166, 241
ApeKI GCWGC 3 cut(s) 229, 445, 559
Asp700I GAANNNNTTC 1 cut(s) 345
AspLEI GCGC 3 cut(s) 186, 487, 542
AspS9I GGNCC 3 cut(s) 242, 304, 505
AsuC2I CCSGG 3 cut(s) 276, 291, 520
AsuHPI GGTGA 3 cut(s) 85, 127, 589
AvaII GGWCC 2 cut(s) 304, 505
AxyI CCTNAGG 1 cut(s) 509
BanI GGYRCC 1 cut(s) 183
BbvI GCAGC 3 cut(s) 241, 432, 546
BccI CCATC 1 cut(s) 585
BceAI ACGGC 1 cut(s) 85
BcgI CGANNNNNNTGC 2 cut(s) 526, 560
BciT130I CCWGG 2 cut(s) 121, 326
BcnI CCSGG 3 cut(s) 276, 291, 520
BfmI CTRYAG 2 cut(s) 227, 560
BfoI RGCGCY 1 cut(s) 187
BisI GCNGC 5 cut(s) 199, 230, 446, 483, 560
BlsI GCNGC 5 cut(s) 200, 231, 447, 484, 561
Bme1390I CCNGG 5 cut(s) 121, 276, 291, 326, 520
Bme18I GGWCC 2 cut(s) 304, 505
BmgBI CACGTC 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 242, 304, 505
BmiI GGNNCC 4 cut(s) 185, 244, 259, 306
BmrFI CCNGG 5 cut(s) 121, 276, 291, 326, 520
BmrI ACTGGG 1 cut(s) 346
BmsI GCATC 1 cut(s) 168
BmuI ACTGGG 1 cut(s) 346
BpuMI CCSGG 3 cut(s) 276, 291, 520
Bsa29I ATCGAT 1 cut(s) 344
BsaHI GRCGYC 4 cut(s) 174, 184, 294, 399
BsaJI CCNNGG 1 cut(s) 108
BsaWI WCCGGW 1 cut(s) 502
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 352
Bse21I CCTNAGG 1 cut(s) 509
BseAI TCCGGA 1 cut(s) 502
BseBI CCWGG 2 cut(s) 121, 326
BseCI ATCGAT 1 cut(s) 344
BseDI CCNNGG 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 351, 500
BseNI ACTGG 1 cut(s) 352
BseXI GCAGC 3 cut(s) 241, 432, 546
BseYI CCCAGC 1 cut(s) 547
BsgI GTGCAG 1 cut(s) 220
Bsh1236I CGCG 2 cut(s) 482, 487
Bsh1285I CGRYCG 2 cut(s) 219, 465
BshFI GGCC 2 cut(s) 168, 243
BshNI GGYRCC 1 cut(s) 183
BshVI ATCGAT 1 cut(s) 344
BsiEI CGRYCG 2 cut(s) 219, 465
BsiSI CCGG 4 cut(s) 276, 291, 503, 519
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 340
BsnI GGCC 2 cut(s) 168, 243
Bsp13I TCCGGA 1 cut(s) 502
Bsp143I GATC 4 cut(s) 6, 216, 405, 466
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 9 cut(s) 105, 157, 199, 272, 381, 448, 461, 482, 529
BspANI GGCC 2 cut(s) 168, 243
BspCNI CTCAG 2 cut(s) 350, 501
BspDI ATCGAT 1 cut(s) 344
BspEI TCCGGA 1 cut(s) 502
BspFNI CGCG 2 cut(s) 482, 487
BspLI GGNNCC 4 cut(s) 185, 244, 259, 306
BspMAI CTGCAG 2 cut(s) 231, 564
BspPI GGATC 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 183
BsrI ACTGG 1 cut(s) 352
BssECI CCNNGG 1 cut(s) 108
BssMI GATC 4 cut(s) 6, 216, 405, 466
BssNI GRCGYC 4 cut(s) 174, 184, 294, 399
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 2 cut(s) 121, 326
Bst4CI ACNGT 3 cut(s) 33, 133, 283
Bst6I CTCTTC 1 cut(s) 566
BstACI GRCGYC 4 cut(s) 174, 184, 294, 399
BstC8I GCNNGC 2 cut(s) 256, 538
BstDEI CTNAG 2 cut(s) 337, 509
BstDSI CCRYGG 1 cut(s) 108
BstFNI CGCG 2 cut(s) 482, 487
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 3 cut(s) 186, 487, 542
BstKTI GATC 4 cut(s) 9, 219, 408, 469
BstMBI GATC 4 cut(s) 6, 216, 405, 466
BstMCI CGRYCG 2 cut(s) 219, 465
BstMWI GCNNNNNNNGC 3 cut(s) 165, 380, 565
BstNI CCWGG 2 cut(s) 121, 326
BstSCI CCNGG 5 cut(s) 119, 274, 289, 324, 518
BstSFI CTRYAG 2 cut(s) 227, 560
BstUI CGCG 2 cut(s) 482, 487
BstV1I GCAGC 3 cut(s) 241, 432, 546
Bsu15I ATCGAT 1 cut(s) 344
Bsu36I CCTNAGG 1 cut(s) 509
BsuRI GGCC 2 cut(s) 168, 243
BsuTUI ATCGAT 1 cut(s) 344
BtgI CCRYGG 1 cut(s) 108
BtrI CACGTC 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 549
BtsIMutI CAGTG 2 cut(s) 279, 549
Cac8I GCNNGC 2 cut(s) 256, 538
CfoI GCGC 3 cut(s) 186, 487, 542
Cfr13I GGNCC 3 cut(s) 242, 304, 505
ClaI ATCGAT 1 cut(s) 344
CseI GACGC 2 cut(s) 283, 388
Csp6I GTAC 2 cut(s) 34, 52
CviAII CATG 2 cut(s) 88, 109
CviQI GTAC 2 cut(s) 34, 52
DdeI CTNAG 2 cut(s) 337, 509
DinI GGCGCC 1 cut(s) 185
DpnI GATC 4 cut(s) 8, 218, 407, 468
DpnII GATC 4 cut(s) 6, 216, 405, 466
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
Eco130I CCWWGG 1 cut(s) 108
Eco47I GGWCC 2 cut(s) 304, 505
Eco57I CTGAAG 1 cut(s) 178
Eco81I CCTNAGG 1 cut(s) 509
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 2 cut(s) 119, 324
EcoT14I CCWWGG 1 cut(s) 108
EgeI GGCGCC 1 cut(s) 185
EheI GGCGCC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 108
FaeI CATG 2 cut(s) 91, 112
FaiI YATR 4 cut(s) 14, 89, 110, 332
FaqI GGGAC 1 cut(s) 340
FatI CATG 2 cut(s) 87, 108
FauI CCCGC 4 cut(s) 112, 279, 388, 441
Fnu4HI GCNGC 5 cut(s) 199, 230, 446, 483, 560
Fsp4HI GCNGC 5 cut(s) 199, 230, 446, 483, 560
GlaI GCGC 3 cut(s) 185, 486, 541
GluI GCNGC 5 cut(s) 199, 230, 446, 483, 560
GsaI CCCAGC 1 cut(s) 551
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 2 cut(s) 168, 243
HapII CCGG 4 cut(s) 276, 291, 503, 519
HgaI GACGC 2 cut(s) 283, 388
HhaI GCGC 3 cut(s) 186, 487, 542
Hin1I GRCGYC 4 cut(s) 174, 184, 294, 399
Hin1II CATG 2 cut(s) 91, 112
Hin6I GCGC 3 cut(s) 184, 485, 540
HinP1I GCGC 3 cut(s) 184, 485, 540
HinfI GANTC 4 cut(s) 78, 116, 341, 346
HpaII CCGG 4 cut(s) 276, 291, 503, 519
HphI GGTGA 3 cut(s) 85, 127, 589
Hpy166II GTNNAC 1 cut(s) 19
Hpy188I TCNGA 3 cut(s) 208, 340, 599
Hpy188III TCNNGA 3 cut(s) 403, 409, 503
Hpy8I GTNNAC 1 cut(s) 19
Hpy99I CGWCG 5 cut(s) 59, 167, 179, 398, 404
HpyAV CCTTC 2 cut(s) 394, 547
HpyCH4III ACNGT 3 cut(s) 33, 133, 283
HpyCH4IV ACGT 4 cut(s) 57, 62, 174, 389
HpyCH4V TGCA 3 cut(s) 229, 237, 562
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 380, 565
HpyF3I CTNAG 2 cut(s) 337, 509
HpySE526I ACGT 4 cut(s) 57, 62, 174, 389
Hsp92I GRCGYC 4 cut(s) 174, 184, 294, 399
Hsp92II CATG 2 cut(s) 91, 112
HspAI GCGC 3 cut(s) 184, 485, 540
KasI GGCGCC 1 cut(s) 183
Kpn2I TCCGGA 1 cut(s) 502
Kzo9I GATC 4 cut(s) 6, 216, 405, 466
LmnI GCTCC 2 cut(s) 263, 556
Lsp1109I GCAGC 3 cut(s) 241, 432, 546
LweI GCATC 1 cut(s) 168
MaeII ACGT 4 cut(s) 57, 62, 174, 389
MaeIII GTNAC 3 cut(s) 58, 133, 577
MalI GATC 4 cut(s) 8, 218, 407, 468
MboI GATC 4 cut(s) 6, 216, 405, 466
MboII GAAGA 3 cut(s) 313, 461, 583
MfeI CAATTG 1 cut(s) 310
MluCI AATT 1 cut(s) 310
Mly113I GGCGCC 1 cut(s) 184
MmeI TCCRAC 1 cut(s) 387
MnlI CCTC 6 cut(s) 149, 272, 326, 380, 504, 567
MroI TCCGGA 1 cut(s) 502
MroXI GAANNNNTTC 1 cut(s) 345
MspI CCGG 4 cut(s) 276, 291, 503, 519
MspR9I CCNGG 5 cut(s) 121, 276, 291, 326, 520
MunI CAATTG 1 cut(s) 310
MvaI CCWGG 2 cut(s) 121, 326
MvnI CGCG 2 cut(s) 482, 487
MwoI GCNNNNNNNGC 3 cut(s) 165, 380, 565
NarI GGCGCC 1 cut(s) 184
NciI CCSGG 3 cut(s) 276, 291, 520
NcoI CCATGG 1 cut(s) 108
NdeII GATC 4 cut(s) 6, 216, 405, 466
NlaIII CATG 2 cut(s) 91, 112
NlaIV GGNNCC 4 cut(s) 185, 244, 259, 306
NmuCI GTSAC 3 cut(s) 58, 133, 577
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 171
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 4 cut(s) 78, 116, 341, 346
Pfl23II CGTACG 1 cut(s) 33
PkrI GCNGC 5 cut(s) 200, 231, 447, 484, 561
Ple19I CGATCG 1 cut(s) 219
PluTI GGCGCC 1 cut(s) 187
Psp6I CCWGG 2 cut(s) 119, 324
PspFI CCCAGC 1 cut(s) 547
PspGI CCWGG 2 cut(s) 119, 324
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 4 cut(s) 185, 244, 259, 306
PspPI GGNCC 3 cut(s) 242, 304, 505
PstI CTGCAG 2 cut(s) 231, 564
PvuI CGATCG 1 cut(s) 219
RsaI GTAC 2 cut(s) 35, 53
RsaNI GTAC 2 cut(s) 34, 52
SatI GCNGC 5 cut(s) 199, 230, 446, 483, 560
Sau3AI GATC 4 cut(s) 6, 216, 405, 466
Sau96I GGNCC 3 cut(s) 242, 304, 505
ScrFI CCNGG 5 cut(s) 121, 276, 291, 326, 520
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 2 cut(s) 227, 560
SfoI GGCGCC 1 cut(s) 185
SinI GGWCC 2 cut(s) 304, 505
Sse9I AATT 1 cut(s) 310
SsiI CCGC 9 cut(s) 105, 157, 199, 272, 381, 448, 461, 482, 529
SspDI GGCGCC 1 cut(s) 183
StyD4I CCNGG 5 cut(s) 119, 274, 289, 324, 518
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 3 cut(s) 33, 133, 283
TaiI ACGT 4 cut(s) 60, 65, 177, 392
TaqI TCGA 4 cut(s) 177, 344, 408, 465
TasI AATT 1 cut(s) 310
TauI GCSGC 2 cut(s) 201, 485
TfiI GAWTC 4 cut(s) 78, 116, 341, 346
TscAI CASTG 2 cut(s) 286, 549
TseFI GTSAC 3 cut(s) 58, 133, 577
TseI GCWGC 3 cut(s) 229, 445, 559
Tsp45I GTSAC 3 cut(s) 58, 133, 577
TspRI CASTG 2 cut(s) 286, 549
VpaK11BI GGWCC 2 cut(s) 304, 505
XmnI GAANNNNTTC 1 cut(s) 345
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.