FvH4_3g40530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004161
Physical Location & Seq
Reverse (-)
34186994 .. 34189371
2378 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g40530.t1

Sequence Viewer

Length: 1263 bp
ATGGAGGCCATTGAAGCCTTCACGGAATCGACCCAACAGTTTTTCGACAACCTCATCCACCGCCGCGCAAACCGGAATCCGATTGAAATACTGAAGCGATTGCAAAGAGAGACGTTTTCAGATTTGATGAGGCTGAGAGAAAGGCAAGACAAGGTTGAGAGGATGGTGGCCTTATTTGGGTCTTCTAAAGGAAGCCCATTTCAAGGAGGCAATACCCGTTTGAAGGGTGAGGTTGATTTCTTGGGGGGTTTGTTATGGAAGCATTGTAATGATGATGGGGGTGGTCAAGATTGTGATGGTCTTAGTAGAGCTGGGATTAGAAGTGGGGTTGATTTTAGGTTGAGTTTTGAAACTGCGGTGAGGGAGAGTGATGGTCTTGTGTGTGAGTTTGTGGCCGGGCAGAAGCACAGGAGTGGTGTTTGTGATGTTTCAGAATGTGGTGTTTCTCTAGCTAAGGTGTGTTATACGGCATATGTGTGCAATTGGTTGTCTTGCATTGCGATTCCAGTGGGATCTCGGTGCAGGGACGTCGCGGTTCTTACTAGTCCTTCCCATCAGGGAAAGGGCCTAACCGATCGATCATCATTCGGACCACCTCTGCTAAATGAGCATAATGGCAGTGCAATTGGCATAATGGTGAAAAATGCGAATGTGGTTGTTGCTTCATTGGGTCAGTCTATTTCAGGACTTGGAATGCAAGTAGGTTCAGGTAGAGTTGAGCACTGCTTGAGCACTTTTGGGCAAATTATCTGTCAACTTCCCGGAAACACCAAACTCTCGCTTCTTGGTCTTCACCGAATGCCCAAATCATCAAGTGAACGTGTCAATCACAGTGCTCCTTGCGTCCCATCAGGCAGTTCGCTACATCATACATCCAGTCCGACAATGGCTGATGCATCTGCTCCACCAGTAAGAACAAACTCAAAAGAAAACGTGTCAGCAATTGGATCCGTAGCTCTGATGTTGGAATCTGAACTTGACGAGACCACAAAGATTGGGGGTTGGATTGAGATGAAGAAATCGAACCCCAGGCATTTAGAATGGGCTGTGTCTGTGTCGGATGACTCTGAAGATTCAGTTGGATGGGGAATAAGTCTAAATGGGATGGTTGGAGGTCCAAATAGCTGTGACCAATTTCAGATCGAATCATACTTGAAATTTAACTTAAGTGAGAGATTCAGTGTAAAGCCTGGTGTTGTACAAATAATGGATGGCAATGCCAAAATGACTGCCTTTATGCTCAAGTCTAACTGGTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

421

Amino Acids

45.46

Weight (kDa)

6.83

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 531
AccII CGCG 2 cut(s) 66, 533
AciI CCGC 4 cut(s) 61, 64, 356, 533
AclWI GGATC 3 cut(s) 520, 942, 955
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 1 cut(s) 1157
AcuI CTGAAG 2 cut(s) 113, 1089
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 1 cut(s) 1200
AfiI CCNNNNNNNGG 3 cut(s) 177, 203, 223
AflII CTTAAG 1 cut(s) 1165
AflIII ACRYGT 2 cut(s) 820, 933
AgsI TTSAA 6 cut(s) 14, 86, 203, 223, 350, 1156
AhlI ACTAGT 1 cut(s) 542
AjnI CCWGG 2 cut(s) 1028, 1189
AjuI GAANNNNNNNTTGG 2 cut(s) 1062, 1094
AleI CACNNNNGTG 1 cut(s) 411
AluBI AGCT 4 cut(s) 311, 452, 956, 1125
AluI AGCT 4 cut(s) 311, 452, 956, 1125
Alw21I GWGCWC 3 cut(s) 723, 734, 838
Alw26I GTCTC 2 cut(s) 104, 977
AlwI GGATC 3 cut(s) 520, 942, 955
AoxI GGCC 4 cut(s) 6, 168, 393, 565
ApoI RAATTY 1 cut(s) 1157
ArsI GACNNNNNNTTYG 2 cut(s) 736, 768
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 3 cut(s) 565, 590, 1115
AsuC2I CCSGG 2 cut(s) 397, 762
AsuHPI GGTGA 4 cut(s) 239, 370, 649, 785
AvaII GGWCC 2 cut(s) 590, 1115
BamHI GGATCC 1 cut(s) 947
BbsI GAAGAC 2 cut(s) 174, 782
Bbv12I GWGCWC 3 cut(s) 723, 734, 838
BccI CCATC 9 cut(s) 157, 269, 290, 365, 561, 856, 1077, 1099, 1205
BceAI ACGGC 1 cut(s) 483
BcgI CGANNNNNNTGC 2 cut(s) 511, 545
BciT130I CCWGG 2 cut(s) 1030, 1191
BcnI CCSGG 2 cut(s) 397, 762
BcoDI GTCTC 2 cut(s) 104, 977
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 2 cut(s) 449, 543
BfrI CTTAAG 1 cut(s) 1165
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
Bme1390I CCNGG 4 cut(s) 397, 762, 1030, 1191
Bme18I GGWCC 2 cut(s) 590, 1115
BmgT120I GGNCC 3 cut(s) 565, 590, 1115
BmiI GGNNCC 1 cut(s) 949
BmrFI CCNGG 4 cut(s) 397, 762, 1030, 1191
BmsI GCATC 2 cut(s) 883, 905
BpiI GAAGAC 2 cut(s) 174, 782
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 2 cut(s) 748, 1226
BpuMI CCSGG 2 cut(s) 397, 762
Bsa29I ATCGAT 1 cut(s) 577
BsaHI GRCGYC 1 cut(s) 528
BsaI GGTCTC 1 cut(s) 977
BsaJI CCNNGG 1 cut(s) 1028
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 203, 223
Bse1I ACTGG 4 cut(s) 506, 876, 908, 1256
Bse3DI GCAATG 2 cut(s) 495, 1222
BseBI CCWGG 2 cut(s) 1030, 1191
BseCI ATCGAT 1 cut(s) 577
BseDI CCNNGG 1 cut(s) 1028
BseGI GGATG 7 cut(s) 54, 168, 872, 1066, 1088, 1110, 1216
BseLI CCNNNNNNNGG 3 cut(s) 177, 203, 223
BseMI GCAATG 2 cut(s) 495, 1222
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 4 cut(s) 506, 876, 908, 1256
BseYI CCCAGC 1 cut(s) 311
BsgI GTGCAG 1 cut(s) 541
Bsh1236I CGCG 2 cut(s) 66, 533
Bsh1285I CGRYCG 1 cut(s) 577
BshFI GGCC 4 cut(s) 8, 170, 395, 567
BshVI ATCGAT 1 cut(s) 577
BsiEI CGRYCG 1 cut(s) 577
BsiHKAI GWGCWC 3 cut(s) 723, 734, 838
BsiSI CCGG 3 cut(s) 73, 396, 762
BslFI GGGAC 2 cut(s) 539, 830
BslI CCNNNNNNNGG 3 cut(s) 177, 203, 223
BsmAI GTCTC 2 cut(s) 104, 977
BsmBI CGTCTC 1 cut(s) 104
BsmFI GGGAC 2 cut(s) 539, 830
BsmI GAATGC 2 cut(s) 699, 804
BsnI GGCC 4 cut(s) 8, 170, 395, 567
Bso31I GGTCTC 1 cut(s) 977
Bsp1286I GDGCHC 3 cut(s) 723, 734, 838
Bsp1407I TGTACA 1 cut(s) 1198
Bsp143I GATC 5 cut(s) 512, 574, 578, 947, 1140
BspACI CCGC 4 cut(s) 61, 64, 356, 533
BspANI GGCC 4 cut(s) 8, 170, 395, 567
BspCNI CTCAG 1 cut(s) 126
BspDI ATCGAT 1 cut(s) 577
BspFNI CGCG 2 cut(s) 66, 533
BspLI GGNNCC 1 cut(s) 949
BspPI GGATC 3 cut(s) 520, 942, 955
BspTI CTTAAG 1 cut(s) 1165
BspTNI GGTCTC 1 cut(s) 977
BsrDI GCAATG 2 cut(s) 495, 1222
BsrGI TGTACA 1 cut(s) 1198
BsrI ACTGG 4 cut(s) 506, 876, 908, 1256
BssECI CCNNGG 1 cut(s) 1028
BssMI GATC 5 cut(s) 512, 574, 578, 947, 1140
BssNI GRCGYC 1 cut(s) 528
Bst2UI CCWGG 2 cut(s) 1030, 1191
Bst4CI ACNGT 2 cut(s) 39, 833
BstACI GRCGYC 1 cut(s) 528
BstAFI CTTAAG 1 cut(s) 1165
BstAUI TGTACA 1 cut(s) 1198
BstDEI CTNAG 3 cut(s) 134, 302, 453
BstF5I GGATG 7 cut(s) 54, 168, 872, 1066, 1088, 1110, 1216
BstFNI CGCG 2 cut(s) 66, 533
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 5 cut(s) 515, 577, 581, 950, 1143
BstMAI GTCTC 2 cut(s) 104, 977
BstMBI GATC 5 cut(s) 512, 574, 578, 947, 1140
BstMCI CGRYCG 1 cut(s) 577
BstMWI GCNNNNNNNGC 2 cut(s) 14, 607
BstNI CCWGG 2 cut(s) 1030, 1191
BstSCI CCNGG 4 cut(s) 395, 760, 1028, 1189
BstUI CGCG 2 cut(s) 66, 533
BstV2I GAAGAC 2 cut(s) 174, 782
BstX2I RGATCY 2 cut(s) 512, 947
BstYI RGATCY 2 cut(s) 512, 947
Bsu15I ATCGAT 1 cut(s) 577
BsuRI GGCC 4 cut(s) 8, 170, 395, 567
BsuTUI ATCGAT 1 cut(s) 577
BtsCI GGATG 7 cut(s) 54, 168, 872, 1066, 1088, 1110, 1216
BtsI GCAGTG 2 cut(s) 625, 721
BtsIMutI CAGTG 5 cut(s) 513, 625, 721, 838, 1186
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 3 cut(s) 565, 590, 1115
ClaI ATCGAT 1 cut(s) 577
CseI GACGC 1 cut(s) 832
Csp6I GTAC 1 cut(s) 1199
CspCI CAANNNNNGTGG 2 cut(s) 976, 1011
CviQI GTAC 1 cut(s) 1199
DdeI CTNAG 3 cut(s) 134, 302, 453
DpnI GATC 5 cut(s) 514, 576, 580, 949, 1142
DpnII GATC 5 cut(s) 512, 574, 578, 947, 1140
EaeI YGGCCR 1 cut(s) 393
Eco31I GGTCTC 1 cut(s) 977
Eco47I GGWCC 2 cut(s) 590, 1115
Eco57I CTGAAG 2 cut(s) 113, 1089
EcoO109I RGGNCCY 1 cut(s) 565
EcoRII CCWGG 2 cut(s) 1028, 1189
EcoT22I ATGCAT 1 cut(s) 898
Esp3I CGTCTC 1 cut(s) 104
FaiI YATR 9 cut(s) 256, 465, 472, 474, 612, 632, 870, 1150, 1238
FaqI GGGAC 2 cut(s) 539, 830
FauNDI CATATG 1 cut(s) 472
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 7 cut(s) 41, 175, 859, 1073, 1095, 1117, 1223
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 2 cut(s) 449, 543
GlaI GCGC 1 cut(s) 67
GluI GCNGC 1 cut(s) 64
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 4 cut(s) 8, 170, 395, 567
HapII CCGG 3 cut(s) 73, 396, 762
HgaI GACGC 1 cut(s) 832
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 528
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 755
HindII GTYRAC 1 cut(s) 755
HinfI GANTC 8 cut(s) 26, 76, 502, 968, 1064, 1073, 1145, 1176
HpaII CCGG 3 cut(s) 73, 396, 762
HphI GGTGA 4 cut(s) 239, 370, 649, 785
Hpy166II GTNNAC 2 cut(s) 755, 818
Hpy188III TCNNGA 2 cut(s) 287, 684
Hpy8I GTNNAC 2 cut(s) 755, 818
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 3 cut(s) 28, 217, 558
HpyCH4III ACNGT 2 cut(s) 39, 833
HpyCH4IV ACGT 4 cut(s) 113, 528, 820, 933
HpyCH4V TGCA 7 cut(s) 103, 480, 495, 522, 623, 697, 896
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 607
HpyF3I CTNAG 3 cut(s) 134, 302, 453
HpySE526I ACGT 4 cut(s) 113, 528, 820, 933
Hsp92I GRCGYC 1 cut(s) 528
HspAI GCGC 1 cut(s) 66
Kzo9I GATC 5 cut(s) 512, 574, 578, 947, 1140
LmnI GCTCC 2 cut(s) 841, 907
LweI GCATC 2 cut(s) 883, 905
MaeI CTAG 2 cut(s) 449, 543
MaeII ACGT 4 cut(s) 113, 528, 820, 933
MaeIII GTNAC 1 cut(s) 1127
MalI GATC 5 cut(s) 514, 576, 580, 949, 1142
MboI GATC 5 cut(s) 512, 574, 578, 947, 1140
MboII GAAGA 4 cut(s) 174, 782, 1027, 1082
MfeI CAATTG 3 cut(s) 481, 624, 942
MflI RGATCY 2 cut(s) 512, 947
MhlI GDGCHC 3 cut(s) 723, 734, 838
MluCI AATT 6 cut(s) 481, 624, 744, 942, 1133, 1157
MlyI GAGTC 1 cut(s) 1058
MmeI TCCRAC 6 cut(s) 905, 945, 983, 1038, 1060, 1090
MnlI CCTC 8 cut(s) 62, 123, 153, 200, 223, 354, 606, 1106
Mph1103I ATGCAT 1 cut(s) 898
MseI TTAA 2 cut(s) 1161, 1166
MslI CAYNNNNRTG 4 cut(s) 267, 411, 475, 635
MspCI CTTAAG 1 cut(s) 1165
MspI CCGG 3 cut(s) 73, 396, 762
MspR9I CCNGG 4 cut(s) 397, 762, 1030, 1191
MunI CAATTG 3 cut(s) 481, 624, 942
Mva1269I GAATGC 2 cut(s) 699, 804
MvaI CCWGG 2 cut(s) 1030, 1191
MvnI CGCG 2 cut(s) 66, 533
MwoI GCNNNNNNNGC 2 cut(s) 14, 607
NciI CCSGG 2 cut(s) 397, 762
NdeI CATATG 1 cut(s) 472
NdeII GATC 5 cut(s) 512, 574, 578, 947, 1140
NlaIV GGNNCC 1 cut(s) 949
NmuCI GTSAC 1 cut(s) 1127
NsiI ATGCAT 1 cut(s) 898
OliI CACNNNNGTG 1 cut(s) 411
PctI GAATGC 2 cut(s) 699, 804
PfeI GAWTC 7 cut(s) 26, 76, 502, 968, 1073, 1145, 1176
PfoI TCCNGGA 1 cut(s) 760
PkrI GCNGC 1 cut(s) 65
Ple19I CGATCG 1 cut(s) 577
PleI GAGTC 1 cut(s) 1058
PpsI GAGTC 1 cut(s) 1058
Psp6I CCWGG 2 cut(s) 1028, 1189
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 1028, 1189
PspN4I GGNNCC 1 cut(s) 949
PspPI GGNCC 3 cut(s) 565, 590, 1115
PsuI RGATCY 2 cut(s) 512, 947
PvuI CGATCG 1 cut(s) 577
RsaI GTAC 1 cut(s) 1200
RsaNI GTAC 1 cut(s) 1199
RseI CAYNNNNRTG 4 cut(s) 267, 411, 475, 635
SaqAI TTAA 2 cut(s) 1161, 1166
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 5 cut(s) 512, 574, 578, 947, 1140
Sau96I GGNCC 3 cut(s) 565, 590, 1115
SchI GAGTC 1 cut(s) 1058
ScrFI CCNGG 4 cut(s) 397, 762, 1030, 1191
SduI GDGCHC 3 cut(s) 723, 734, 838
SfaNI GCATC 2 cut(s) 883, 905
SinI GGWCC 2 cut(s) 590, 1115
SmiMI CAYNNNNRTG 4 cut(s) 267, 411, 475, 635
SmlI CTYRAG 3 cut(s) 727, 1165, 1241
SmoI CTYRAG 3 cut(s) 727, 1165, 1241
SpeI ACTAGT 1 cut(s) 542
Sse9I AATT 6 cut(s) 481, 624, 744, 942, 1133, 1157
SsiI CCGC 4 cut(s) 61, 64, 356, 533
SspMI CTAG 2 cut(s) 449, 543
StyD4I CCNGG 4 cut(s) 395, 760, 1028, 1189
TaaI ACNGT 2 cut(s) 39, 833
TaiI ACGT 4 cut(s) 116, 531, 823, 936
TaqI TCGA 5 cut(s) 29, 45, 577, 1022, 1143
TasI AATT 6 cut(s) 481, 624, 744, 942, 1133, 1157
TatI WGTACW 1 cut(s) 1198
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 7 cut(s) 26, 76, 502, 968, 1073, 1145, 1176
Tru1I TTAA 2 cut(s) 1161, 1166
Tru9I TTAA 2 cut(s) 1161, 1166
TscAI CASTG 5 cut(s) 513, 625, 728, 838, 1186
TseFI GTSAC 1 cut(s) 1127
Tsp45I GTSAC 1 cut(s) 1127
TspDTI ATGAA 2 cut(s) 654, 1028
TspGWI ACGGA 2 cut(s) 38, 940
TspRI CASTG 5 cut(s) 513, 625, 728, 838, 1186
Vha464I CTTAAG 1 cut(s) 1165
VpaK11BI GGWCC 2 cut(s) 590, 1115
XapI RAATTY 1 cut(s) 1157
XcmI CCANNNNNNNNNTGG 1 cut(s) 883
XspI CTAG 2 cut(s) 449, 543
ZraI GACGTC 1 cut(s) 529
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.