FvH4_3g41250

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003692
Physical Location & Seq
Forward (+)
34692569 .. 34702172
9604 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41250.t2

Sequence Viewer

Length: 2751 bp
ATGCAGTCTACGCAGGCCATTCTCAAGCAATATTTTGGGTTCTCTTCATTTCGGCCATACCAGGAGGAAGTGATTGAGAAAATCATAGCTGGAAGGGACTCTTTGATTGTCATGGCCACTGGAAGTGGCAAGTCTTTGTGTTATCAAGTGCCTCCTTTGGTTGTTGGGAAGACTGGTGTGGTTGTCAGCCCTCTTATATCCTTAATGCAAGATCAGGTGATGTCTTTGAAACAAAGAGGGATCAGAGCTGAATATATGGGTAGTAGTCAAACTGATAGCACTGTCCAGAGCCGAGCCGAGAGTGGTCAGTTTGATATCTTGTACATGACCCCTGAAAAGGCATGTGTGATTCCTGTCAGCTTCTGGTCAAAATTACTAAGGGCTGGGATCTGCTTGTTTGCTGTTGATGAAGCACACTGCATATCAGAATGGGGCCATGATTTCAGGGTAGAATACAAGCAATTGGACAAATTACGTGGCATTCTTGTTGGTGTGCCGTTTATTGGCTTAACCGCAACTGCTACTGAAAAGGTTCGAATGGACATTGTTAATTCCTTGAAGTTGGAAAACCCATATGTCAAAATAGGCTCATTTGATCGTGGAAATCTGTTCTATGGTGTCAAGTTATTCAATCGGACTCAATCATTTGTTCATGACCTGGTTCAAGAAGTTTCCAAATTTGTACGCACAGATGGTTCAACTATAATTTACTGCACGACAATTAAAGACGTTGATCAGGTATTCAATTCCCTGAAGGAGGTGGGCATTAAGGCTGGAATCTACCATGGTCAAATGGATAATAAAGCTCGTGCAGAATCCCATAGATTATTCATAAGAGATGAACTTGATGTCATGGTTGCTACTATTGCTTTTGGCATGGGTATTGATAAGCCAAACATAAGACAAGTAATCCACTATGGCTGCCCAAAGAGTTTGGAATCTTATTACCAGGAAAGTGGACGATGTGGTAGAGATGGTATGGCTTCTGTCTGCTGGCTCTATTACACAAGAAGCGACTTCGCTAAAGCTGACTTTTATGCTGGAGAGTGTCAAACAGAAAGTCAACGAAGAGCTGTTGTAGAGTCATTGATGGCAGCACAACAATATTGCTTATCAACAACTTGCAGAAGGAAGTTCTTGCTTGGTCACTTTGGGGAAATCTTTCCATCTGATAGATGTGGTAATTGTGATAATTGTATTTCCTCAAAGGAGAGGGACATGTCTAGAGAAGCATTTCTTCTAATGGCTTGCATTCAATCATGTCGGGGTAAATGGGGTCTGAATATGCCTGTAGATATTCTTCGTGGCTCTAGGGCCAAAAAGATTCTTGATGCCCAATATGACAAGCTTCCGCTGCATGGACTTGGGAAAGACTATTCAGCAAATTGGTGGAAAGCACTCGCTTACCAATTAATCTCTTCTGGTTATTTGATGGAGATGGTATCTGATATATACAGAACTGTAAGCGTCAGTCGGAAAGGGGAGCAATTTCTAAGTTCTGCTGGACCTGATCATCAACCGCCTCTGGTGTTGCCAGTGACCAGTGAAATGGTAGATGATGAGGACAATAAAAGTACATCAGGTGAAGTCGGAGAAATTAAAGGTTTGTCTACTTTGGAATGCGAAGGATTTTCAGAGGCTGAGAAGCAACTGTATCATTCACTTCTTGAAGAGAGAAGGAAGCTTGCAAGAAGCCTAGGAACTGCACCATATGCCATTTGTGGTGATCAAGCCATCAAAAAAATTGCTTTAACAAGACCATCAACCAGAGCAAGGTTAGCTAACATCGATGGCGTTAACCAGCACCTTGTAGTGGCACACGGTAATCATTTTCTCCAGATCATTCGGCATCTATCACAAGGACTAAATCTTTCGTTGGATGGAGAGGCACCTGTAGAAACTGCTGTTACGAAAAAAGTATATCCAGTACCTAACCACCATCAGAGAAAGTTAACACCGGCAAAGTTTGAAGCTTGGAGAATGTGGCATGAAGATGGCCTCTCAATTCAGAAAATTGCTAATTTCCCTGGTAGAGCTGCTCCTATTAAAGAGCAGACTGTTCAAGAATATCTGATGGAAGCTGCTCAAGAAGGATTTGCAATTGATTGGGTCAGATTACGCAATGAGGTTGGACTAACACAAAAAATTATGTCAGATATTCAGTGTGCCATTTCAAAGGTTGGCTCTAGAGAAAAGTTAAAGCCTATCAAAGACGAATTACCAGAAGATGTAAGTTATGCACACATCAAGACTTTCTTGACAATGGAAAAGTGTGGGATCTCGCTGGATGGGACTCCACCTAATTACCACAATGCAGGAAAAGCCGATCAACTTGTCAACAAGGAAACTGAAATGTCACCGAGTTCTATTCATACATCCCCCATGGAAGAACCTCATGAAGTGAAAACCTCAGGCCGAGATTCAGATGCTTACAGTTTGGATAAAAATACAGAAGCAGCTTCCCTTCCCTTCAATGGAGAACAAGGTTTAAAGCTACCTGAAGTACATTTTGAGGACTTGCTCTCAAAGAAACGTCAAAAGCTTGGTAGTCCCAAGGAGGAAAGTAAAACTACACTGAAGGCAACCGAGAAATCCATATTGGACTGGCTTAATACCAAAAAAGGAGTTACTCTCTCCGAGATTTTGGAGCACTTCAATGGATCTGAAAAAGAATCTGTCATTGAACTCCTCACTAGCCTTGAAAGTGAATTTGAGATATACAGAAAGAACAATATGTATTTGGTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

917

Amino Acids

102.75

Weight (kDa)

6.78

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 16 - 173 7.9e-06 Type III restriction enzyme, res subunit
DEAD PF00270 18 - 179 1.9e-24 DEAD/DEAH box helicase
Helicase_C PF00271 227 - 326 1.3e-21 Helicase conserved C-terminal domain
RecQ_Zn_bind PF16124 338 - 399 6.9e-14 RecQ zinc-binding
RQC PF09382 403 - 499 4.2e-24 RQC domain
HRDC PF00570 549 - 615 1.1e-13 HRDC domain
HTH_40 PF14493 657 - 752 3.7e-18 Helix-turn-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1888
AccI GTMKAC 2 cut(s) 8, 1610
AciI CCGC 3 cut(s) 513, 1352, 1520
AclWI GGATC 4 cut(s) 248, 395, 2285, 2668
AcoI YGGCCR 2 cut(s) 53, 114
AcsI RAATTY 2 cut(s) 677, 2708
AcuI CTGAAG 3 cut(s) 773, 2520, 2597
AfaI GTAC 5 cut(s) 323, 684, 1576, 1929, 2505
AfiI CCNNNNNNNGG 7 cut(s) 337, 503, 757, 1358, 1773, 1813, 2474
AflIII ACRYGT 1 cut(s) 1218
AjnI CCWGG 4 cut(s) 60, 657, 948, 2026
AjuI GAANNNNNNNTTGG 2 cut(s) 2582, 2614
Alw21I GWGCWC 1 cut(s) 2652
AlwI GGATC 4 cut(s) 248, 395, 2285, 2668
AlwNI CAGNNNCTG 2 cut(s) 363, 1640
AoxI GGCC 7 cut(s) 15, 53, 114, 433, 1314, 1996, 2413
ApeKI GCWGC 6 cut(s) 921, 1094, 1354, 2036, 2081, 2456
ApoI RAATTY 2 cut(s) 677, 2708
ArsI GACNNNNNNTTYG 2 cut(s) 1045, 1077
AseI ATTAAT 1 cut(s) 1412
Asp700I GAANNNNTTC 3 cut(s) 531, 1161, 1233
AspA2I CCTAGG 1 cut(s) 1696
AspS9I GGNCC 3 cut(s) 433, 1314, 1505
AsuHPI GGTGA 4 cut(s) 229, 1595, 1736, 2349
AsuII TTCGAA 1 cut(s) 535
AvaII GGWCC 1 cut(s) 1505
AvrII CCTAGG 1 cut(s) 1696
AxyI CCTNAGG 1 cut(s) 2410
BalI TGGCCA 1 cut(s) 116
BanI GGYRCC 1 cut(s) 1888
BarI GAAGNNNNNNTAC 2 cut(s) 1284, 1316
BauI CACGAG 1 cut(s) 807
BbsI GAAGAC 1 cut(s) 176
Bbv12I GWGCWC 1 cut(s) 2652
BbvI GCAGC 6 cut(s) 908, 1106, 1341, 2023, 2068, 2468
BceAI ACGGC 1 cut(s) 481
BciT130I CCWGG 4 cut(s) 62, 659, 950, 2028
BclI TGATCA 3 cut(s) 733, 1510, 1726
BfaI CTAG 5 cut(s) 1224, 1311, 1697, 2187, 2694
BfmI CTRYAG 2 cut(s) 1290, 1893
BisI GCNGC 6 cut(s) 922, 1095, 1355, 2037, 2082, 2457
BlnI CCTAGG 1 cut(s) 1696
BlsI GCNGC 6 cut(s) 923, 1096, 1356, 2038, 2083, 2458
Bme1390I CCNGG 4 cut(s) 62, 659, 950, 2028
Bme18I GGWCC 1 cut(s) 1505
BmgT120I GGNCC 3 cut(s) 433, 1314, 1505
BmiI GGNNCC 2 cut(s) 434, 1890
BmrFI CCNGG 4 cut(s) 62, 659, 950, 2028
BmsI GCATC 3 cut(s) 1321, 1858, 2416
BpiI GAAGAC 1 cut(s) 176
BplI GAGNNNNNCTC 2 cut(s) 2616, 2648
BpmI CTGGAG 2 cut(s) 1062, 1820
Bpu14I TTCGAA 1 cut(s) 535
BpuEI CTTGAG 2 cut(s) 8, 2070
Bsa29I ATCGAT 1 cut(s) 1788
BsaAI YACGTR 1 cut(s) 476
BsaJI CCNNGG 5 cut(s) 784, 1696, 2026, 2382, 2553
Bsc4I CCNNNNNNNGG 7 cut(s) 337, 503, 757, 1358, 1773, 1813, 2474
Bse118I RCCGGY 1 cut(s) 1957
Bse1I ACTGG 6 cut(s) 124, 178, 1535, 1542, 1925, 2609
Bse21I CCTNAGG 1 cut(s) 2410
Bse3DI GCAATG 1 cut(s) 2128
BseBI CCWGG 4 cut(s) 62, 659, 950, 2028
BseCI ATCGAT 1 cut(s) 1788
BseDI CCNNGG 5 cut(s) 784, 1696, 2026, 2382, 2553
BseGI GGATG 3 cut(s) 1885, 2293, 2375
BseLI CCNNNNNNNGG 7 cut(s) 337, 503, 757, 1358, 1773, 1813, 2474
BseMI GCAATG 1 cut(s) 2128
BseMII CTCAG 2 cut(s) 1632, 2424
BseNI ACTGG 6 cut(s) 124, 178, 1535, 1542, 1925, 2609
BseRI GAGGAG 1 cut(s) 2678
BseXI GCAGC 6 cut(s) 908, 1106, 1341, 2023, 2068, 2468
BseYI CCCAGC 1 cut(s) 383
BsgI GTGCAG 3 cut(s) 697, 831, 1689
BshFI GGCC 7 cut(s) 17, 55, 116, 435, 1316, 1998, 2415
BshNI GGYRCC 1 cut(s) 1888
BshVI ATCGAT 1 cut(s) 1788
BsiHKAI GWGCWC 1 cut(s) 2652
BsiSI CCGG 1 cut(s) 1958
BslFI GGGAC 4 cut(s) 110, 1229, 2305, 2535
BslI CCNNNNNNNGG 7 cut(s) 337, 503, 757, 1358, 1773, 1813, 2474
BsmFI GGGAC 4 cut(s) 110, 1229, 2305, 2535
BsmI GAATGC 3 cut(s) 480, 1251, 1625
BsnI GGCC 7 cut(s) 17, 55, 116, 435, 1316, 1998, 2415
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 1 cut(s) 2652
Bsp1407I TGTACA 1 cut(s) 321
Bsp19I CCATGG 2 cut(s) 784, 2382
BspACI CCGC 3 cut(s) 513, 1352, 1520
BspANI GGCC 7 cut(s) 17, 55, 116, 435, 1316, 1998, 2415
BspCNI CTCAG 2 cut(s) 1633, 2423
BspDI ATCGAT 1 cut(s) 1788
BspHI TCATGA 2 cut(s) 652, 2395
BspLI GGNNCC 2 cut(s) 434, 1890
BspPI GGATC 4 cut(s) 248, 395, 2285, 2668
BspQI GCTCTTC 1 cut(s) 1063
BspT104I TTCGAA 1 cut(s) 535
BspT107I GGYRCC 1 cut(s) 1888
BsrDI GCAATG 1 cut(s) 2128
BsrFI RCCGGY 1 cut(s) 1957
BsrGI TGTACA 1 cut(s) 321
BsrI ACTGG 6 cut(s) 124, 178, 1535, 1542, 1925, 2609
BssAI RCCGGY 1 cut(s) 1957
BssECI CCNNGG 5 cut(s) 784, 1696, 2026, 2382, 2553
BssSI CACGAG 1 cut(s) 807
BssT1I CCWWGG 4 cut(s) 784, 1696, 2382, 2553
Bst2BI CACGAG 1 cut(s) 807
Bst2UI CCWGG 4 cut(s) 62, 659, 950, 2028
Bst4CI ACNGT 6 cut(s) 283, 1462, 1653, 1823, 2059, 2435
Bst6I CTCTTC 4 cut(s) 49, 1063, 1423, 1665
BstAPI GCANNNNNTGC 1 cut(s) 1712
BstAUI TGTACA 1 cut(s) 321
BstBAI YACGTR 1 cut(s) 476
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 4 cut(s) 15, 995, 1249, 1686
BstDEI CTNAG 4 cut(s) 377, 1493, 1641, 2410
BstDSI CCRYGG 2 cut(s) 784, 2382
BstENI CCTNNNNNAGG 1 cut(s) 755
BstF5I GGATG 3 cut(s) 1885, 2293, 2375
BstMWI GCNNNNNNNGC 6 cut(s) 10, 866, 1354, 1712, 1778, 2321
BstNI CCWGG 4 cut(s) 62, 659, 950, 2028
BstNSI RCATGY 2 cut(s) 345, 1222
BstSCI CCNGG 4 cut(s) 60, 657, 948, 2026
BstSFI CTRYAG 2 cut(s) 1290, 1893
BstV1I GCAGC 6 cut(s) 908, 1106, 1341, 2023, 2068, 2468
BstV2I GAAGAC 1 cut(s) 176
BstX2I RGATCY 3 cut(s) 387, 2277, 2660
BstXI CCANNNNNNTGG 2 cut(s) 956, 1549
BstYI RGATCY 3 cut(s) 387, 2277, 2660
Bsu15I ATCGAT 1 cut(s) 1788
Bsu36I CCTNAGG 1 cut(s) 2410
BsuRI GGCC 7 cut(s) 17, 55, 116, 435, 1316, 1998, 2415
BsuTUI ATCGAT 1 cut(s) 1788
BtgI CCRYGG 2 cut(s) 784, 2382
BtsCI GGATG 3 cut(s) 1885, 2293, 2375
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 7 cut(s) 117, 279, 415, 1542, 1549, 2168, 2573
Cac8I GCNNGC 4 cut(s) 15, 995, 1249, 1686
CaiI CAGNNNCTG 2 cut(s) 363, 1640
CciI TCATGA 2 cut(s) 652, 2395
Cfr10I RCCGGY 1 cut(s) 1957
Cfr13I GGNCC 3 cut(s) 433, 1314, 1505
ClaI ATCGAT 1 cut(s) 1788
CseI GACGC 1 cut(s) 1456
CsiI ACCWGGT 1 cut(s) 657
Csp6I GTAC 5 cut(s) 322, 683, 1575, 1928, 2504
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviQI GTAC 5 cut(s) 322, 683, 1575, 1928, 2504
DdeI CTNAG 4 cut(s) 377, 1493, 1641, 2410
DraI TTTAAA 1 cut(s) 2490
EaeI YGGCCR 2 cut(s) 53, 114
Eam1104I CTCTTC 4 cut(s) 49, 1063, 1423, 1665
EarI CTCTTC 4 cut(s) 49, 1063, 1423, 1665
Eco130I CCWWGG 4 cut(s) 784, 1696, 2382, 2553
Eco32I GATATC 1 cut(s) 316
Eco47I GGWCC 1 cut(s) 1505
Eco57I CTGAAG 3 cut(s) 773, 2520, 2597
Eco81I CCTNAGG 1 cut(s) 2410
EcoNI CCTNNNNNAGG 1 cut(s) 755
EcoRII CCWGG 4 cut(s) 60, 657, 948, 2026
EcoRV GATATC 1 cut(s) 316
EcoT14I CCWWGG 4 cut(s) 784, 1696, 2382, 2553
ErhI CCWWGG 4 cut(s) 784, 1696, 2382, 2553
FalI AAGNNNNNCTT 6 cut(s) 85, 117, 1221, 1253, 2240, 2272
FaqI GGGAC 4 cut(s) 110, 1229, 2305, 2535
FauNDI CATATG 2 cut(s) 574, 1711
FbaI TGATCA 3 cut(s) 733, 1510, 1726
FblI GTMKAC 2 cut(s) 8, 1610
Fnu4HI GCNGC 6 cut(s) 922, 1095, 1355, 2037, 2082, 2457
FokI GGATG 3 cut(s) 1892, 2300, 2362
Fsp4HI GCNGC 6 cut(s) 922, 1095, 1355, 2037, 2082, 2457
FspBI CTAG 5 cut(s) 1224, 1311, 1697, 2187, 2694
GluI GCNGC 6 cut(s) 922, 1095, 1355, 2037, 2082, 2457
GsaI CCCAGC 1 cut(s) 387
GsuI CTGGAG 2 cut(s) 1062, 1820
HaeIII GGCC 7 cut(s) 17, 55, 116, 435, 1316, 1998, 2415
HapII CCGG 1 cut(s) 1958
HgaI GACGC 1 cut(s) 1456
HincII GTYRAC 4 cut(s) 1064, 1798, 1953, 2338
HindII GTYRAC 4 cut(s) 1064, 1798, 1953, 2338
HindIII AAGCTT 4 cut(s) 1346, 1682, 1971, 2540
HpaI GTTAAC 2 cut(s) 1798, 1953
HpaII CCGG 1 cut(s) 1958
HphI GGTGA 4 cut(s) 229, 1595, 1736, 2349
Hpy166II GTNNAC 7 cut(s) 9, 959, 1064, 1611, 1798, 1953, 2338
Hpy8I GTNNAC 7 cut(s) 9, 959, 1064, 1611, 1798, 1953, 2338
HpyAV CCTTC 9 cut(s) 87, 748, 1122, 1619, 1671, 2084, 2474, 2479, 2572
HpyCH4III ACNGT 6 cut(s) 283, 1462, 1653, 1823, 2059, 2435
HpyCH4IV ACGT 3 cut(s) 475, 729, 2533
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 866, 1354, 1712, 1778, 2321
HpyF3I CTNAG 4 cut(s) 377, 1493, 1641, 2410
HpySE526I ACGT 3 cut(s) 475, 729, 2533
Ksp22I TGATCA 3 cut(s) 733, 1510, 1726
KspAI GTTAAC 2 cut(s) 1798, 1953
LguI GCTCTTC 1 cut(s) 1063
LmnI GCTCC 3 cut(s) 1483, 2044, 2647
Lsp1109I GCAGC 6 cut(s) 908, 1106, 1341, 2023, 2068, 2468
LweI GCATC 3 cut(s) 1321, 1858, 2416
MabI ACCWGGT 1 cut(s) 657
MaeI CTAG 5 cut(s) 1224, 1311, 1697, 2187, 2694
MaeII ACGT 3 cut(s) 475, 729, 2533
MaeIII GTNAC 5 cut(s) 1145, 1537, 1906, 2355, 2626
MfeI CAATTG 2 cut(s) 461, 2100
MflI RGATCY 3 cut(s) 387, 2277, 2660
MhlI GDGCHC 1 cut(s) 2652
MlsI TGGCCA 1 cut(s) 116
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 4 cut(s) 92, 631, 1091, 2287
MmeI TCCRAC 5 cut(s) 543, 1454, 1570, 1857, 2110
Mox20I TGGCCA 1 cut(s) 116
MroXI GAANNNNTTC 3 cut(s) 531, 1161, 1233
MscI TGGCCA 1 cut(s) 116
MslI CAYNNNNRTG 2 cut(s) 1992, 2655
Msp20I TGGCCA 1 cut(s) 116
MspA1I CMGCKG 1 cut(s) 1354
MspI CCGG 1 cut(s) 1958
MspR9I CCNGG 4 cut(s) 62, 659, 950, 2028
MunI CAATTG 2 cut(s) 461, 2100
Mva1269I GAATGC 3 cut(s) 480, 1251, 1625
MvaI CCWGG 4 cut(s) 62, 659, 950, 2028
MwoI GCNNNNNNNGC 6 cut(s) 10, 866, 1354, 1712, 1778, 2321
NcoI CCATGG 2 cut(s) 784, 2382
NdeI CATATG 2 cut(s) 574, 1711
NlaIV GGNNCC 2 cut(s) 434, 1890
NmeAIII GCCGAG 3 cut(s) 317, 322, 2441
NmuCI GTSAC 3 cut(s) 1145, 1537, 2355
NspI RCATGY 2 cut(s) 345, 1222
NspV TTCGAA 1 cut(s) 535
PagI TCATGA 2 cut(s) 652, 2395
PciI ACATGT 1 cut(s) 1218
PciSI GCTCTTC 1 cut(s) 1063
PctI GAATGC 3 cut(s) 480, 1251, 1625
PdmI GAANNNNTTC 3 cut(s) 531, 1161, 1233
PfeI GAWTC 7 cut(s) 349, 777, 815, 938, 1324, 2420, 2672
PkrI GCNGC 6 cut(s) 923, 1096, 1356, 2038, 2083, 2458
PleI GAGTC 4 cut(s) 92, 631, 1090, 2287
PpsI GAGTC 4 cut(s) 92, 631, 1090, 2287
Ppu21I YACGTR 1 cut(s) 476
PscI ACATGT 1 cut(s) 1218
PshBI ATTAAT 1 cut(s) 1412
Psp6I CCWGG 4 cut(s) 60, 657, 948, 2026
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 4 cut(s) 60, 657, 948, 2026
PspN4I GGNNCC 2 cut(s) 434, 1890
PspPI GGNCC 3 cut(s) 433, 1314, 1505
PstNI CAGNNNCTG 2 cut(s) 363, 1640
PsuI RGATCY 3 cut(s) 387, 2277, 2660
RsaI GTAC 5 cut(s) 323, 684, 1576, 1929, 2505
RsaNI GTAC 5 cut(s) 322, 683, 1575, 1928, 2504
RseI CAYNNNNRTG 2 cut(s) 1992, 2655
SapI GCTCTTC 1 cut(s) 1063
SatI GCNGC 6 cut(s) 922, 1095, 1355, 2037, 2082, 2457
Sau96I GGNCC 3 cut(s) 433, 1314, 1505
SchI GAGTC 4 cut(s) 92, 631, 1091, 2287
ScrFI CCNGG 4 cut(s) 62, 659, 950, 2028
SduI GDGCHC 1 cut(s) 2652
SexAI ACCWGGT 1 cut(s) 657
SfaNI GCATC 3 cut(s) 1321, 1858, 2416
SfcI CTRYAG 2 cut(s) 1290, 1893
SfuI TTCGAA 1 cut(s) 535
SinI GGWCC 1 cut(s) 1505
SmiMI CAYNNNNRTG 2 cut(s) 1992, 2655
SmlI CTYRAG 2 cut(s) 23, 2085
SmoI CTYRAG 2 cut(s) 23, 2085
SsiI CCGC 3 cut(s) 513, 1352, 1520
SspI AATATT 2 cut(s) 32, 1106
SspMI CTAG 5 cut(s) 1224, 1311, 1697, 2187, 2694
StyD4I CCNGG 4 cut(s) 60, 657, 948, 2026
StyI CCWWGG 4 cut(s) 784, 1696, 2382, 2553
TaaI ACNGT 6 cut(s) 283, 1462, 1653, 1823, 2059, 2435
TaiI ACGT 3 cut(s) 478, 732, 2536
TaqI TCGA 2 cut(s) 535, 1788
TatI WGTACW 3 cut(s) 321, 1574, 2503
TfiI GAWTC 7 cut(s) 349, 777, 815, 938, 1324, 2420, 2672
TscAI CASTG 7 cut(s) 124, 286, 422, 1542, 1549, 2168, 2580
TseFI GTSAC 3 cut(s) 1145, 1537, 2355
TseI GCWGC 6 cut(s) 921, 1094, 1354, 2036, 2081, 2456
Tsp45I GTSAC 3 cut(s) 1145, 1537, 2355
TspDTI ATGAA 8 cut(s) 36, 423, 641, 820, 855, 2004, 2360, 2412
TspRI CASTG 7 cut(s) 124, 286, 422, 1542, 1549, 2168, 2580
VpaK11BI GGWCC 1 cut(s) 1505
VspI ATTAAT 1 cut(s) 1412
XagI CCTNNNNNAGG 1 cut(s) 755
XapI RAATTY 2 cut(s) 677, 2708
XbaI TCTAGA 2 cut(s) 1223, 2186
XceI RCATGY 2 cut(s) 345, 1222
XmaJI CCTAGG 1 cut(s) 1696
XmiI GTMKAC 2 cut(s) 8, 1610
XmnI GAANNNNTTC 3 cut(s) 531, 1161, 1233
XspI CTAG 5 cut(s) 1224, 1311, 1697, 2187, 2694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.