Rorug05G0428600

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003692
Physical Location & Seq
Reverse (-)
59604973 .. 59606733
1761 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0428600.1

Sequence Viewer

Length: 1761 bp
ATGGAAACAACAATTGGGAGAAAGAGGAAGGGGAGGACCCATACATTAGAAGCCGAAGAAGAAAATCGTATGCTAAAAAAGAAGATGCAAACAACACGGGGGAGAAAGAGGAAGGAGAGAACACATACATTAGAAGAAGAAGAAAATTGCAGGCAAACAAAAAAAGAGAAAAGCAAAATAGACGTGAGTTTCTCCAAGAGGCGTAAAGGGTTGTTTGGTAAGGCTGCTCGTTTGTGCAAGGATTTCAATGACACACGAATTGCGGTTCTCGTGGTTTCGAAAGCAAGTAGACCTTATGCTTTTGGACACTCTTCGGTGGATGAAGTTCTCAATGATCTCTACCACATTCAACCGACAACTACTGAATCTGTCGAGGGGCTCAATCTGATGAGGCACCAACACGAGCAAAAAATGTTGATGGTTGTGAAGGAAGAGATTGAGAACGAATTGAGAGCCTGTCAAACTGTTGAGGGACTTGCATCGTTGAGAGAAAAATATTTGAAGCTTGATGAAGATATCAAAAAGAGGTTGAGAAGTTGTTCCTCTGAAGAAATCAGTTTTGAGGGTGGTGTGATTGATCAGAAGTACAATATCGACATCATTAATGTTGGTGATGAGGAATGTTCTTTAGGGTCAAGGCATGAAAGTAACTATAATAATATTTCAATGGCTGGTGGTCTAAGATTAGATGGAGACACTGGTGGTGAGGGGTATTGTTCTGGGTTAGGGCATAGGGGTTCAACTACTGGATTTAGGGGTAATTCTTTGCTGGCTTCAACTGAAAGTGATTATGGTATTCATAATGATGGTTTAACAATAAATGGAGACATTGATGGTGATGGATGTTCTTTAAGGTTATGGCATGATGTTGGTGATAATTCTTTGCTAGCTTCAACTAACTATTCTGTTAATGATGGTGGGTTAGGGTTAGGGCGTGAAGTTAGTGGTAATTCTTTGAGGGGTTTAACTACTGGCATTAATGGTAATTCTTTGTGTTCAACTACAAGTAATTATGGTGATGATGGGGGTTTAAGATTAAATGGAGATATTAATCTAGACGGTTGTTCTTTAGGGTTATGGCATGAAGTAAGTGATAATTCTTTGAGGGATTCAACTACTGACATTAGGGGTAATGTTTTGTTGGCTTCAACTACAAGTAATTATGGTGTTGATGGGGATTTAAGATTAAATGGAGACATCGATGCCGAGGGATGTTCTTTAGGGTTATGGTATGAAGTCAGTGACAATCCTTTGAGGGATTCATCTACTGACATTAGTGGTAATGTTTTGTTGGCTTCAACTACAAGTAATAATGGTGTTGATGGGGATTTAAGATTAAATGGAGACATGGATGCCGAGGGATGTTCTTTAGGGTTCTGGTATGAAGATAGTGATAATCCTTTGGGGGATTCAACTACTGACACTATTGGTAATGTTTTGTTGGCTTCAACTACAAGTAATGATGGTGTTGATGGTGGTTTAAGATTGAATGGAGGCACTGATGCTGATGAGGGATTCTCTTTAGGGTTAGGACATGAAGTTTATGGTAATTCTTTGGGGAATTTTAAAATTTCTGGTGACTTTGATGATTCACCTCCAGCTTGGGTTTGTTCTTCTTCCAATGGTGCCTCGGAGACGGTGGAGTCATCATCAGATTTTGTGCAATCCAATACCAACAAGGGATATTACAGCTACTTGATGAAAGGAGACATAGACAGTGGTCCTTACAGTTACCAGGACTCATGTAACTTTTCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

586

Amino Acids

63.56

Weight (kDa)

4.74

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 57 - 101 2.2e-07 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1642
AccB1I GGYRCC 2 cut(s) 393, 1625
AccI GTMKAC 1 cut(s) 289
AciI CCGC 1 cut(s) 263
AcsI RAATTY 2 cut(s) 1561, 1569
AcuI CTGAAG 1 cut(s) 567
AfaI GTAC 1 cut(s) 587
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 1 cut(s) 1734
AjuI GAANNNNNNNTTGG 1 cut(s) 29
AluBI AGCT 4 cut(s) 505, 890, 1601, 1692
AluI AGCT 4 cut(s) 505, 890, 1601, 1692
Alw26I GTCTC 6 cut(s) 687, 819, 1188, 1338, 1628, 1701
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 2 cut(s) 1561, 1569
AseI ATTAAT 3 cut(s) 603, 978, 1050
Asp700I GAANNNNTTC 1 cut(s) 538
AspS9I GGNCC 2 cut(s) 36, 1721
AsuHPI GGTGA 7 cut(s) 623, 716, 848, 884, 1028, 1584, 1589
AsuII TTCGAA 1 cut(s) 278
AsuNHI GCTAGC 1 cut(s) 886
AvaII GGWCC 2 cut(s) 36, 1721
BanI GGYRCC 2 cut(s) 393, 1625
BanII GRGCYC 1 cut(s) 381
BauI CACGAG 2 cut(s) 269, 401
BbvI GCAGC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 1736
BclI TGATCA 1 cut(s) 577
BcoDI GTCTC 6 cut(s) 687, 819, 1188, 1338, 1628, 1701
BfaI CTAG 2 cut(s) 887, 1055
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 1 cut(s) 1736
Bme18I GGWCC 2 cut(s) 36, 1721
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 36, 1721
BmiI GGNNCC 3 cut(s) 38, 395, 1627
BmrFI CCNGG 1 cut(s) 1736
BmsI GCATC 5 cut(s) 75, 488, 1192, 1342, 1492
BmtI GCTAGC 1 cut(s) 890
BoxI GACNNNNGTC 1 cut(s) 1719
BplI GAGNNNNNCTC 2 cut(s) 1502, 1534
BpmI CTGGAG 1 cut(s) 1581
Bpu14I TTCGAA 1 cut(s) 278
Bsa29I ATCGAT 1 cut(s) 1200
BsaBI GATNNNNATC 1 cut(s) 1050
BsaJI CCNNGG 3 cut(s) 1206, 1356, 1629
Bse1I ACTGG 3 cut(s) 703, 751, 976
Bse8I GATNNNNATC 1 cut(s) 1050
BseBI CCWGG 1 cut(s) 1736
BseCI ATCGAT 1 cut(s) 1200
BseDI CCNNGG 3 cut(s) 1206, 1356, 1629
BseGI GGATG 5 cut(s) 325, 848, 1217, 1357, 1367
BseJI GATNNNNATC 1 cut(s) 1050
BseNI ACTGG 3 cut(s) 703, 751, 976
BseXI GCAGC 1 cut(s) 211
BshNI GGYRCC 2 cut(s) 393, 1625
BshVI ATCGAT 1 cut(s) 1200
BslFI GGGAC 1 cut(s) 486
BsmAI GTCTC 6 cut(s) 687, 819, 1188, 1338, 1628, 1701
BsmBI CGTCTC 1 cut(s) 1628
BsmFI GGGAC 1 cut(s) 486
Bsp119I TTCGAA 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 2 cut(s) 334, 577
BspACI CCGC 1 cut(s) 263
BspDI ATCGAT 1 cut(s) 1200
BspLI GGNNCC 3 cut(s) 38, 395, 1627
BspOI GCTAGC 1 cut(s) 890
BspT104I TTCGAA 1 cut(s) 278
BspT107I GGYRCC 2 cut(s) 393, 1625
BsrI ACTGG 3 cut(s) 703, 751, 976
BssECI CCNNGG 3 cut(s) 1206, 1356, 1629
BssMI GATC 2 cut(s) 334, 577
BssSI CACGAG 2 cut(s) 269, 401
Bst2BI CACGAG 2 cut(s) 269, 401
Bst2UI CCWGG 1 cut(s) 1736
Bst4CI ACNGT 5 cut(s) 466, 1061, 1639, 1718, 1730
Bst6I CTCTTC 2 cut(s) 316, 426
BstBI TTCGAA 1 cut(s) 278
BstC8I GCNNGC 3 cut(s) 152, 771, 888
BstDEI CTNAG 1 cut(s) 680
BstF5I GGATG 5 cut(s) 325, 848, 1217, 1357, 1367
BstKTI GATC 2 cut(s) 337, 580
BstMAI GTCTC 6 cut(s) 687, 819, 1188, 1338, 1628, 1701
BstMBI GATC 2 cut(s) 334, 577
BstNI CCWGG 1 cut(s) 1736
BstPAI GACNNNNGTC 1 cut(s) 1719
BstSCI CCNGG 1 cut(s) 1734
BstV1I GCAGC 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 1200
BsuTUI ATCGAT 1 cut(s) 1200
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 5 cut(s) 325, 848, 1217, 1357, 1367
BtsIMutI CAGTG 4 cut(s) 696, 1246, 1497, 1723
Cac8I GCNNGC 3 cut(s) 152, 771, 888
Cfr13I GGNCC 2 cut(s) 36, 1721
ClaI ATCGAT 1 cut(s) 1200
Csp6I GTAC 1 cut(s) 586
CviAII CATG 6 cut(s) 641, 863, 1082, 1348, 1535, 1743
CviQI GTAC 1 cut(s) 586
DdeI CTNAG 1 cut(s) 680
DpnI GATC 2 cut(s) 336, 579
DpnII GATC 2 cut(s) 334, 577
DraI TTTAAA 1 cut(s) 1567
DrdI GACNNNNNNGTC 1 cut(s) 1642
DseDI GACNNNNNNGTC 1 cut(s) 1642
Eam1104I CTCTTC 2 cut(s) 316, 426
EarI CTCTTC 2 cut(s) 316, 426
Eco24I GRGCYC 1 cut(s) 381
Eco32I GATATC 1 cut(s) 517
Eco47I GGWCC 2 cut(s) 36, 1721
Eco57I CTGAAG 1 cut(s) 567
EcoO109I RGGNCCY 1 cut(s) 36
EcoRII CCWGG 1 cut(s) 1734
EcoRV GATATC 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 381
Esp3I CGTCTC 1 cut(s) 1628
FaeI CATG 6 cut(s) 644, 866, 1085, 1351, 1538, 1746
FalI AAGNNNNNCTT 2 cut(s) 277, 309
FaqI GGGAC 1 cut(s) 486
FatI CATG 6 cut(s) 640, 862, 1081, 1347, 1534, 1742
FbaI TGATCA 1 cut(s) 577
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 5 cut(s) 332, 855, 1224, 1364, 1374
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 2 cut(s) 887, 1055
GluI GCNGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 1581
Hin1II CATG 6 cut(s) 644, 866, 1085, 1351, 1538, 1746
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 8 cut(s) 365, 1109, 1259, 1409, 1515, 1589, 1643, 1739
HphI GGTGA 7 cut(s) 623, 716, 848, 884, 1028, 1584, 1589
Hpy166II GTNNAC 1 cut(s) 290
Hpy188I TCNGA 5 cut(s) 387, 547, 582, 1633, 1654
Hpy188III TCNNGA 1 cut(s) 1055
Hpy8I GTNNAC 1 cut(s) 290
HpyAV CCTTC 3 cut(s) 22, 106, 421
HpyCH4III ACNGT 5 cut(s) 466, 1061, 1639, 1718, 1730
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 5 cut(s) 88, 150, 237, 479, 1663
HpyF3I CTNAG 1 cut(s) 680
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 6 cut(s) 644, 866, 1085, 1351, 1538, 1746
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 2 cut(s) 334, 577
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 5 cut(s) 75, 488, 1192, 1342, 1492
MaeI CTAG 2 cut(s) 887, 1055
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 5 cut(s) 647, 1241, 1577, 1730, 1745
MalI GATC 2 cut(s) 336, 579
MboI GATC 2 cut(s) 334, 577
MfeI CAATTG 1 cut(s) 12
MhlI GDGCHC 1 cut(s) 381
MlyI GAGTC 2 cut(s) 1652, 1733
MroXI GAANNNNTTC 1 cut(s) 538
MslI CAYNNNNRTG 1 cut(s) 804
MspR9I CCNGG 1 cut(s) 1736
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 1736
NdeII GATC 2 cut(s) 334, 577
NheI GCTAGC 1 cut(s) 886
NlaIII CATG 6 cut(s) 644, 866, 1085, 1351, 1538, 1746
NlaIV GGNNCC 3 cut(s) 38, 395, 1627
NmeAIII GCCGAG 2 cut(s) 1231, 1381
NmuCI GTSAC 2 cut(s) 1241, 1577
NspV TTCGAA 1 cut(s) 278
PdmI GAANNNNTTC 1 cut(s) 538
PfeI GAWTC 6 cut(s) 365, 1109, 1259, 1409, 1515, 1589
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 2 cut(s) 1651, 1733
PpsI GAGTC 2 cut(s) 1651, 1733
PpuMI RGGWCCY 1 cut(s) 36
PshAI GACNNNNGTC 1 cut(s) 1719
PshBI ATTAAT 3 cut(s) 603, 978, 1050
Psp5II RGGWCCY 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 1734
PspGI CCWGG 1 cut(s) 1734
PspN4I GGNNCC 3 cut(s) 38, 395, 1627
PspPI GGNCC 2 cut(s) 36, 1721
PspPPI RGGWCCY 1 cut(s) 36
RsaI GTAC 1 cut(s) 587
RsaNI GTAC 1 cut(s) 586
RseI CAYNNNNRTG 1 cut(s) 804
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 2 cut(s) 334, 577
Sau96I GGNCC 2 cut(s) 36, 1721
SchI GAGTC 2 cut(s) 1652, 1733
ScrFI CCNGG 1 cut(s) 1736
SduI GDGCHC 1 cut(s) 381
SetI ASST 9 cut(s) 186, 295, 507, 530, 857, 892, 1597, 1603, 1694
SfaNI GCATC 5 cut(s) 75, 488, 1192, 1342, 1492
SfuI TTCGAA 1 cut(s) 278
SinI GGWCC 2 cut(s) 36, 1721
SmiMI CAYNNNNRTG 1 cut(s) 804
SsiI CCGC 1 cut(s) 263
SspI AATATT 2 cut(s) 497, 661
SspMI CTAG 2 cut(s) 887, 1055
StyD4I CCNGG 1 cut(s) 1734
TaaI ACNGT 5 cut(s) 466, 1061, 1639, 1718, 1730
TaiI ACGT 1 cut(s) 186
TaqI TCGA 4 cut(s) 278, 372, 594, 1200
TatI WGTACW 1 cut(s) 585
TfiI GAWTC 6 cut(s) 365, 1109, 1259, 1409, 1515, 1589
TscAI CASTG 4 cut(s) 703, 1246, 1504, 1723
TseFI GTSAC 2 cut(s) 1241, 1577
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 2 cut(s) 1241, 1577
TspRI CASTG 4 cut(s) 703, 1246, 1504, 1723
VpaK11BI GGWCC 2 cut(s) 36, 1721
VspI ATTAAT 3 cut(s) 603, 978, 1050
XapI RAATTY 2 cut(s) 1561, 1569
XbaI TCTAGA 1 cut(s) 1054
XmiI GTMKAC 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 538
XspI CTAG 2 cut(s) 887, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.