Rorug05G0428500

Belongs to the helicase family. RecQ subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003566
Physical Location & Seq
Reverse (-)
59601218 .. 59604192
2975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0428500.1

Sequence Viewer

Length: 453 bp
ATGAAAACCCAATTAGCAGCTTCTCTAATTTTCATGCCCCTTCTTCTTTTCCTTCTCCCAAGTTTAACAATTCATACCAAAGCTGAGGTTATAACCCTAACCGCTGACACCTTCTCCGACAAGGTGAAGGAGAAGGACACTGCATGGTTTGTCAAGTTCTGTGTACCATGGTGTAAACATTGTAAGAACTTGGGGTCATTGTGGGAGGATTTTGGGAAGGCAGTGGAAAGCGAAGATGAAATAGAGGTTGGGGAAGTGGATTGCAGTACGAGTAAACCAGTGTGCTCGAAAGTTGATATTCATTCTTATCCTACATTTAAGCTATTCTTTGATGGAGAAGAAGTCGCCAAATATCAAGGGCCGAGGGATGTTGAATCACTTAAGAATTTTGTCTTAGACCAGGCTGAAGAAGCAGCAAGAAAGGCACATCTTGGCAGTGATAAAGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000018 GO:0000217 GO:0000287 GO:0000400 GO:0000403 GO:0000405 GO:0000723 GO:0000724 GO:0000725 GO:0000731 GO:0000781 GO:0001302 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003682 GO:0003684 GO:0003824 GO:0004003 GO:0004386 GO:0004518 GO:0004527 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005657 GO:0005694 GO:0005730 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006284 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006979 GO:0006996 GO:0007154 GO:0007275 GO:0007568 GO:0007569 GO:0008026 GO:0008094 GO:0008104 GO:0008150 GO:0008152 GO:0008408 GO:0009058 GO:0009059 GO:0009267 GO:0009314 GO:0009378 GO:0009411 GO:0009416 GO:0009605 GO:0009628 GO:0009893 GO:0009987 GO:0009991 GO:0010212 GO:0010225 GO:0010259 GO:0010332 GO:0010604 GO:0010941 GO:0015630 GO:0016043 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030145 GO:0031297 GO:0031323 GO:0031325 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032200 GO:0032356 GO:0032357 GO:0032392 GO:0032501 GO:0032502 GO:0032508 GO:0033036 GO:0033365 GO:0033554 GO:0034504 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0040008 GO:0040009 GO:0042592 GO:0042594 GO:0042623 GO:0042802 GO:0042803 GO:0042981 GO:0042995 GO:0043005 GO:0043067 GO:0043085 GO:0043138 GO:0043140 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044093 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044464 GO:0044806 GO:0044877 GO:0045005 GO:0045911 GO:0045935 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051171 GO:0051173 GO:0051179 GO:0051276 GO:0051336 GO:0051345 GO:0051641 GO:0051716 GO:0051880 GO:0060249 GO:0060255 GO:0060542 GO:0061749 GO:0061820 GO:0061821 GO:0062037 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070035 GO:0070336 GO:0070337 GO:0070727 GO:0071103 GO:0071214 GO:0071478 GO:0071479 GO:0071480 GO:0071496 GO:0071704 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090657 GO:0097159 GO:0097458 GO:0098530 GO:0098687 GO:0104004 GO:0120025 GO:0140097 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902570 GO:1905773
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HRDC HTH_40 Helicase_C RQC RecQ_Zn_bind

Protein Analysis

150

Amino Acids

16.89

Weight (kDa)

5.1

Isoelectric Point (pI)

23.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 30 - 131 4.9e-24 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 92
AciI CCGC 1 cut(s) 102
AcsI RAATTY 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 426
AfaI GTAC 2 cut(s) 165, 268
AflII CTTAAG 1 cut(s) 380
AgsI TTSAA 1 cut(s) 374
AjnI CCWGG 1 cut(s) 399
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 3 cut(s) 20, 83, 322
AluI AGCT 3 cut(s) 20, 83, 322
Alw21I GWGCWC 1 cut(s) 287
AoxI GGCC 1 cut(s) 359
ApeKI GCWGC 2 cut(s) 17, 413
ApoI RAATTY 1 cut(s) 385
AspS9I GGNCC 1 cut(s) 359
AsuHPI GGTGA 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 287
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 2 cut(s) 29, 425
BccI CCATC 1 cut(s) 326
BciT130I CCWGG 1 cut(s) 401
BfrI CTTAAG 1 cut(s) 380
BisI GCNGC 2 cut(s) 18, 414
BlsI GCNGC 2 cut(s) 19, 415
Bme1390I CCNGG 1 cut(s) 401
BmgT120I GGNCC 1 cut(s) 359
BmrFI CCNGG 1 cut(s) 401
Bpu10I CCTNAGC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 167, 362
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 327, 357
Bse1I ACTGG 1 cut(s) 278
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 2 cut(s) 167, 362
BseGI GGATG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 278
BseXI GCAGC 2 cut(s) 29, 425
BshFI GGCC 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 287
BsnI GGCC 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 287
Bsp19I CCATGG 1 cut(s) 167
BspACI CCGC 1 cut(s) 102
BspANI GGCC 1 cut(s) 361
BspCNI CTCAG 1 cut(s) 76
BspTI CTTAAG 1 cut(s) 380
BsrI ACTGG 1 cut(s) 278
BssECI CCNNGG 2 cut(s) 167, 362
BssT1I CCWWGG 1 cut(s) 167
Bst2UI CCWGG 1 cut(s) 401
BstAFI CTTAAG 1 cut(s) 380
BstDEI CTNAG 2 cut(s) 84, 394
BstDSI CCRYGG 1 cut(s) 167
BstF5I GGATG 1 cut(s) 373
BstMWI GCNNNNNNNGC 2 cut(s) 410, 422
BstNI CCWGG 1 cut(s) 401
BstSCI CCNGG 1 cut(s) 399
BstV1I GCAGC 2 cut(s) 29, 425
BsuRI GGCC 1 cut(s) 361
BtgI CCRYGG 1 cut(s) 167
BtsCI GGATG 1 cut(s) 373
BtsI GCAGTG 3 cut(s) 138, 228, 442
BtsIMutI CAGTG 4 cut(s) 138, 228, 285, 442
Cfr13I GGNCC 1 cut(s) 359
Csp6I GTAC 2 cut(s) 164, 267
CviAII CATG 3 cut(s) 34, 144, 168
CviJI RGCY 5 cut(s) 20, 83, 322, 361, 404
CviKI_1 RGCY 5 cut(s) 20, 83, 322, 361, 404
CviQI GTAC 2 cut(s) 164, 267
DdeI CTNAG 2 cut(s) 84, 394
Eco130I CCWWGG 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 399
EcoT14I CCWWGG 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 3 cut(s) 37, 147, 171
FaiI YATR 5 cut(s) 35, 75, 92, 145, 169
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FatI CATG 3 cut(s) 33, 143, 167
Fnu4HI GCNGC 2 cut(s) 18, 414
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 2 cut(s) 18, 414
GluI GCNGC 2 cut(s) 18, 414
HaeIII GGCC 1 cut(s) 361
Hin1II CATG 3 cut(s) 37, 147, 171
HinfI GANTC 1 cut(s) 374
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 3 cut(s) 164, 176, 275
Hpy188I TCNGA 1 cut(s) 118
Hpy8I GTNNAC 3 cut(s) 164, 176, 275
HpyAV CCTTC 6 cut(s) 50, 62, 121, 121, 127, 211
HpyCH4V TGCA 2 cut(s) 143, 264
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 422
HpyF3I CTNAG 2 cut(s) 84, 394
Hsp92II CATG 3 cut(s) 37, 147, 171
LpnPI CCDG 3 cut(s) 291, 386, 413
Lsp1109I GCAGC 2 cut(s) 29, 425
MboII GAAGA 4 cut(s) 35, 245, 350, 419
MhlI GDGCHC 1 cut(s) 287
MluCI AATT 4 cut(s) 11, 27, 69, 385
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 4 cut(s) 79, 199, 238, 357
MseI TTAA 3 cut(s) 65, 318, 381
MspA1I CMGCKG 1 cut(s) 104
MspCI CTTAAG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 401
MvaI CCWGG 1 cut(s) 401
MwoI GCNNNNNNNGC 2 cut(s) 410, 422
NcoI CCATGG 1 cut(s) 167
NlaIII CATG 3 cut(s) 37, 147, 171
NmeAIII GCCGAG 1 cut(s) 387
PfeI GAWTC 1 cut(s) 374
PkrI GCNGC 2 cut(s) 19, 415
PsiI TTATAA 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspPI GGNCC 1 cut(s) 359
RsaI GTAC 2 cut(s) 165, 268
RsaNI GTAC 2 cut(s) 164, 267
SaqAI TTAA 3 cut(s) 65, 318, 381
SatI GCNGC 2 cut(s) 18, 414
Sau96I GGNCC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 401
SduI GDGCHC 1 cut(s) 287
SetI ASST 7 cut(s) 22, 85, 90, 113, 126, 249, 324
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 4 cut(s) 11, 27, 69, 385
SsiI CCGC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 399
StyI CCWWGG 1 cut(s) 167
TaqI TCGA 1 cut(s) 287
TasI AATT 4 cut(s) 11, 27, 69, 385
TfiI GAWTC 1 cut(s) 374
Tru1I TTAA 3 cut(s) 65, 318, 381
Tru9I TTAA 3 cut(s) 65, 318, 381
TscAI CASTG 4 cut(s) 145, 228, 285, 442
TseI GCWGC 2 cut(s) 17, 413
TspDTI ATGAA 5 cut(s) 17, 22, 62, 252, 290
TspRI CASTG 4 cut(s) 145, 228, 285, 442
Vha464I CTTAAG 1 cut(s) 380
XapI RAATTY 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.